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Citations for Thermo Fisher :
2551 - 2600 of 10000+ citations for hsa mir 23b Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... using the SuperScript RT-PCR kit (Invitrogen). They were cloned into the XhoI-AvrII site of the pEOE-attP-eGFP vector using In-Fusion cloning ...
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bioRxiv - Immunology 2023Quote: ... RT-qPCR was performed using PCR master mix (Powerup™ SYBR™ Green Master Mix Catalog: A25741) and real-time PCR system (Applied biosystems™ 7500 Fast Real-Time PCR system). CT values were determined after normalizing to housekeeping gene GAPDH.
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bioRxiv - Genetics 2023Quote: ... qPCR was performed with a RT-PCR detection system (Applied Biosystems, California, USA) using a SYBR Premix Ex Taq Kit (Q711 ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative real-time PCR analysis was performed using a DyNAmo ColorFlash SYBR Green qPCR kit (Thermo Scientific) in a Step One Plus real-time PCR system (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... and a quantitative Real-Time PCR was performed according to the manufacturer’s instructions (MMLV Kit, Life Technologies and Smart SYBRGreen kit ...
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bioRxiv - Microbiology 2024Quote: ... a real time PCR quantification step was performed using Ion Library TaqMan™ Quantitation Kit (Thermo Fisher). Next steps included an emulsion PCR of the pools and loading on 520 chips performed using the automated Ion Chef instrument (Thermo Fisher) ...
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bioRxiv - Microbiology 2024Quote: ... a real time PCR quantification step was performed using Ion Library TaqMan™ Quantitation Kit (Thermo Fisher). Next steps included an emulsion PCR of the pools and loading on 530 chips performed using the automated Ion Chef instrument (Thermo Fisher) ...
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bioRxiv - Microbiology 2024Quote: ... Real-time SYBR-based PCR (qPCR) was carried out with Power SYBR Green Master Mix kit (ThermoFisher) using 12.5 µL Mix Reagent ...
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bioRxiv - Genomics 2022Quote: ... was used for RSV subtype detection with TaqPath 1-Step Multiplex Master Mix (No ROX) on Applied Biosystems 7500 Real-Time PCR System (ABI, Life Technologies, Waltham, MA) as instructed ...
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bioRxiv - Molecular Biology 2023Quote: The mRNA content of AMPKγ3 and TATA-box binding protein (TBP) was determined by real-time PCR using the QuantStudio 7 Flex detection system (Applied Biosystems, CA, USA) as previously described (33) ...
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bioRxiv - Immunology 2024Quote: ... Protein unfolding reactions and fluorescent signal detection were performed in Applied Biosystems 7900 HT Fast Real-Time PCR machine (Applied Biosystems, Thermo Fisher Scientific). For data analyses ...
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bioRxiv - Physiology 2024Quote: ... on cDNA template with a Bio Rad CFX384-well Real-time PCR Detection System and PowerUp SYBR green Master Mix (ThermoFisher Scientific, catalog #A25742). A list of primer sequences for measuring gene expression are provided in table S1 ...
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bioRxiv - Cell Biology 2020Quote: ... A one-step RT-qPCR reaction will be used to amplify the cDNA using the QuantStudio Real-Time PCR System (ThermoFisher Scientific, Waltham, USA). Each sample will be processed in triplicate with beta actin used as a control gene ...
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bioRxiv - Microbiology 2020Quote: The TaqMan real-time RT-PCR assays were performed by using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific, MA, USA). Each 20 μl reaction mix contained 5 μl of 4×Fast Virus 1-Step Master Mix ...
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bioRxiv - Plant Biology 2020Quote: ... RT-qPCR of three biological replications with no-template control (NTC) involved StepOnePlus Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) and FastStart SYBR Green (Roche) ...
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bioRxiv - Molecular Biology 2019Quote: The RT-qPCR analysis was carried out using SDS 2.3 software in 7900HT Fast Real-Time PCR System (Applied Biosystems, Foster City, California). For the detection of mRNA expression ...
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bioRxiv - Cell Biology 2020Quote: ... A total of 10ng cDNA for each sample was used and all RT-qPCR reactions were performed on a ViaA 7 Real-Time PCR System (Life Technologies, Carlsbad, CA) using Brilliant II SYBR Green QPCR Master Mix (Agilent ...
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bioRxiv - Neuroscience 2024Quote: ... RT-qPCR was performed in translucent 96-well plates using the Applied Biosystems 7500-Real-Time PCR System (Applied Biosystems, Waltham, MA, USA). For each well ...
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bioRxiv - Molecular Biology 2024Quote: ... to perform a one-step RT-qPCR in a volume of 20 μl using a QuantStudio3 real-time PCR instrument from ThermoFisher (Waltham, MA, USA). The primers used are provided in the Key Resource Table ...
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bioRxiv - Developmental Biology 2024Quote: ... and analyzed by RT-qPCR using Quant Studio 12K Flex Real Time PCR system version 1.1.2 (Applied Biosystems by Life Technologies, Thermo Fisher Scientific) and validated (efficiency > 1.8 ...
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bioRxiv - Biochemistry 2023Quote: ... The expression of target genes was analyzed via Quantitative real-time (RT) PCR with a QuantaStudio 5 qPCR machine (Applied Biosystems, Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2021Quote: Reverse Transcriptase PCR and Quantitative Real-Time PCR were prepared using the Power SYBR Green Cells-to-Ct Kit (Thermo Scientific) protocol ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA was generated using q-Script cDNA Supermix kit (Quanta Biosciences) and quantitative PCR was performed in triplicates on 7500 Fast Real-Time PCR system (Applied Biosystems) with Perfecta SYBR Green Supermix (Quanta Biosciences) ...
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bioRxiv - Cell Biology 2020Quote: ... total RNA was extracted using Trizol with analysis by real time PCR according to manufacturer’s instructions (One-step VERSO SYBR green PCR kit, Thermo Fisher Scientific). Total RNA was extracted from mouse lung tissue using Trizol with analysis of 25 ng of RNA reverse transcribed and complementary DNA was amplified by real time-PCR using a One-step VERSO SYBR green PCR kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Quantitative R T-PCR was performed according to the protocol supplied by the SYBR Green PCR kit using the 7500 Real-Time PCR System (Applied Biosystems). Cycling conditions were 94°C for 30 s ...
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bioRxiv - Microbiology 2022Quote: ... The PCR was performed using the BioRad CFX96 Real-Time PCR cycler using a DyNAmo Flash SYBR green qRT-PCR kit (Catalog no. F415XL; Thermo Scientific).
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bioRxiv - Molecular Biology 2024Quote: qRT-PCR was performed using the Power SYBR Green 1-step kit and the 7500 Fast Real-Time PCR System (Applied Biosystems). Relative expression was calculated using the comparative Ct method with Actin B (ACTB ...
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bioRxiv - Cancer Biology 2023Quote: ... The cDNA was used as a template for quantitative PCR using KAPA SYBR FAST qPCR Kits (KAPA) and 7300 Real-Time PCR System (Applied Biosystems). Expression levels of target genes were normalized using an mRNA level of the β2-microglobulin gene (B2M ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Real-time PCR was performed using a real-time PCR kit provided with SYBR green fluorophore (Powerup SYBER Green Master Mix, Applied Biosystems) with 600 mM of each primer ...
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bioRxiv - Genomics 2019Quote: ... SH-SY5Y cells were transfected with either the hsa-miR-101-3p mimic (MC11414; Thermo Fisher Scientific, Waltham, MA, USA) or scrambled control (mirVana™ miRNA Mimic ...
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bioRxiv - Molecular Biology 2022Quote: mirVana™ miRNA Mimics and Inhibitors were used for hsa-miR-181a-5p overexpression/inhibition (Ambion™ - 4464066 and 4464084). HeLa cells were transfected with corresponding mirVana™ miRNA in 24 well plates at a density of 20.000 cells per well ...
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bioRxiv - Cancer Biology 2023Quote: ... both shβ7 cells and H295R cells were transfected with control miRNA mimics or hsa-miR-139-5p mimics (Thermofisher, #MC11749) at a final concentration of 10nM ...
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bioRxiv - Neuroscience 2024Quote: ... Primers used were: rno-miR-155-5p (RT:002571, ThermoFisher Scientific), and rno-miR-674-3p (RT:001956 ...
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bioRxiv - Cancer Biology 2023Quote: Quantitative reverse transcription-PCR (qRT-PCR) analysis was done on a QuantStudio Real-Time PCR instrument (ThermoFisher) using PowerUp™ SYBR® Green Master Mix (ThermoFisher) ...
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bioRxiv - Physiology 2024Quote: ... TaqMan-based real time quantitative polymerase chain reactions (qRT-PCR) were then performed using a QuantStudio 7 Pro Real-Time PCR System (ThermoFisher). The housekeeping gene Vinculin was used as an internal control for cDNA quantification and normalization of gene amplified products.
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bioRxiv - Microbiology 2023Quote: ... RNA samples were analyzed by qRT-PCR with Thunderbird Probe One-step qRT-PCR Kit (Toyobo) and QuantStudio 7 Flex Real-time PCR system (Applied Biosystems; Thermo Fisher Scientific). Target RNA levels were normalized to hamster Actb and calculated by the relative standard curve method ...
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bioRxiv - Immunology 2021Quote: ... Real-time qPCR was performed using the ABI PRISM 7000 Sequence Detection System (Applied Biosystems), and SYBR Premix Ex Taq II (Takara ...
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bioRxiv - Immunology 2021Quote: ... real-time polymerase chain reaction was performed with the 7900HT Sequence Detection System (Applied Biosystems) using the KAPA PROBE FAST Universal qPCR kit (Peqlab) ...
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bioRxiv - Neuroscience 2021Quote: ... Quantitative PCR was conducted on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) using 12 ng cDNA per reaction ...
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bioRxiv - Cell Biology 2020Quote: ... standard qRT-PCR was carried out with a StepOnePlus Real-Time PCR system (Applied Biosystems) using TaqMan Fast Universal PCR Master Mix (Applied Biosystems) ...
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bioRxiv - Cell Biology 2020Quote: ... qRT-PCR was performed on Step One Plus Real-Time PCR System (Applied Biosystems, USA) with SYBR Premix Ex Taq II Kit (Clontech Laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... The PCR reaction was performed on a ViiA 7 Real-Time PCR System (Applied Biosystems). Samples were run in triplicate and analyzed using comparative CT (2-ΔΔCT ...
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bioRxiv - Developmental Biology 2021Quote: ... Real-time PCR was performed using Power SYBR Green PCR Master Mix (Applied Biosystems 4367659). Thermal conditions were 2 min at 50 °C ...
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bioRxiv - Immunology 2021Quote: ... cDNA was used for subsequent PCR amplification using the viia7 Real-time PCR system (Thermofisher) and the Fast SYBR Green Master Mix (Thermofisher) ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were analyzed on an ABI 7500 Fast real-time PCR system (Applied Biosystems).
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bioRxiv - Evolutionary Biology 2021Quote: ... Quantitative PCR (qPCR) was performed in a StepOnePlus Real-Time PCR system (Thermo Fisher Scientific). Reaction conditions were optimised using a gradient PCR ...
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bioRxiv - Plant Biology 2022Quote: ... qRT-PCR was performed using a StepOne Real-time PCR Instrument (Applied Biosystems, CA, USA) with KAPA SYBR FAST PCR Master Mix (Kapa Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: ... qRT-PCR was carried out on QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific) with iTaq SYBR Green Supermix (Bio-Rad Laboratories).
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bioRxiv - Molecular Biology 2021Quote: ... qRT-PCR was performed on a QuantStudio Real-Time PCR Light Cycler (Thermo Fisher Scientific) in technical triplicates ...
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bioRxiv - Genomics 2020Quote: ... The PCR was performed in a QuantStudio 5 Real-Time PCR System (Thermo Fisher Scientific) using the following parameters ...