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Citations for Thermo Fisher :
2451 - 2500 of 8294 citations for Rat ERICH6 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... hTERT-RPE1 cells were transfected with donor plasmid and AIO plasmid with the appropriate gRNAs using a Neon transfection system (Thermofisher Scientific). For 5 × 106 cells ...
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Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying essential circRNAsbioRxiv - Molecular Biology 2020Quote: ... Clones were scraped off the LB plates and plasmid DNA was extracted using PureLink™ HiPure Plasmid Maxiprep Kit (ThermoFisher, K210007). The libraries were submitted for next generation sequencing to confirm the coverage and diversity of gRNA and shRNA libraries ...
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bioRxiv - Developmental Biology 2020Quote: ... The next day PcDNA 3.1/ Twist1-BirA*-HA plasmid or PcDNA 3.1/ GFP-BirA*-HA plasmid was transfected into cells using Lipofectamine® 3000 (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... RPE-1 wildtype cells were transfected with the CRISPR/Cas9 plasmid containing the PCNA gRNA and the donor plasmid containing the K164R mutation using the Neon Transfection System (Invitrogen MPK5000) following standard protocols ...
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bioRxiv - Immunology 2021Quote: ... were transformed with the modified transfer vectors and before plasmid DNA extraction using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 1.5 μg of reporter plasmid and 15 ng of Cre-plasmid was mixed with 3 μL Lipofectamine LTX reagent (Invitrogen, #15338100), 1.5 μL PLUS reagent and 100 μL Opti-MEM following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2019Quote: ... This plasmid consists of six inserts and the plasmid cloning vector CloneJet pJET1.2/blunt (2974 bp; Thermo Fisher Scientific, Massachusetts, USA). For the inserts ...
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bioRxiv - Microbiology 2019Quote: Genomic DNA was extracted using the GeneJET Genomic DNA Purification Kit while plasmid DNA was extracted using the GeneJET Plasmid Miniprep Kit (Thermo Scientific). PCR assays were performed using Phusion High-Fidelity PCR Master Mix (Thermo Scientific) ...
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bioRxiv - Genomics 2019Quote: ... were used for sequencing the full length of cloned PCR product after plasmid purification using the GeneJET Plasmid Miniprep Kit (Thermo Scientific).
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bioRxiv - Bioengineering 2021Quote: ... nanobody sequences were PCR amplified with primers containing terminal regions of homology for cloning into a pRset plasmid (Addgene plasmid 3991)(Invitrogen, V35120) in frame and linked to mCherry ...
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bioRxiv - Neuroscience 2021Quote: ... Transient overexpression of VDR in SH-SY5Y cells was performed by transfecting a VDR expression plasmid (pcDNA3-VDR) or a mock control plasmid (pcDNA3) with Lipofectamine 2000 (Invitrogen, USA). For mammalian two-hybrid assays to assess the interaction between VDR and RXR ...
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bioRxiv - Systems Biology 2020Quote: ... We added 800–1000 ng of plasmid (SF2(gDNA) or SF2(cDNA) or SynT) using the Lipofectamine LTX plasmid transfection reagent (ThermoFisher Scientific) and changed the culture media to Opti-MEM™ Reduced Serum Medium (ThermoFisher Scientific) ...
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bioRxiv - Systems Biology 2020Quote: ... We added 800–1000 ng of plasmid (SF2(gDNA) or SF2(cDNA) or SynT) using the Lipofectamine LTX plasmid transfection reagent (ThermoFisher Scientific) and changed the culture media to Opti-MEM™ Reduced Serum Medium (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2021Quote: ... 1 million cells in 6-well plates were transfected with 2 μg of plasmid pT2_FF together with 2 μg of plasmid pCMV(CAT)T7-SB100x and 2 μg of plasmid pcDNA3.1-mCherry using Lipofectamine 2000 (Thermo Fisher, #11668027). After 24 h ...
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bioRxiv - Molecular Biology 2020Quote: ... K562#17 cells were transfected with 32 μg of barcoded IPR-PB-BC plasmid library and 6 μg of PB transposase plasmid using Lipofectamine 2000 (ThermoFisher #11668019). Mock-transfected (without PB transposase ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids were extracted with NucleoSpin Plasmid Transfection-grade (Machery-Nagel) and concentration were measured on Nanodrop ND-1000 (Thermo Fisher Scientific). All plasmids were sequenced by Sanger method (Giga-Genomics).
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected with 1,250 ng of dCas13a m6A editor plasmid and 1,250 ng of crRNA plasmid using 5 μl of Lipofectamine 3000 (Thermo Fisher Scientific) in Opti-MEM I Reduced Serum Media (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected with 1,250 ng of dCas13a m6A editor plasmid and 1,250 ng of crRNA plasmid using 5 μl of Lipofectamine 3000 (Thermo Fisher Scientific) in Opti-MEM I Reduced Serum Media (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... The cDNAs were cloned into pDONR221 plasmid and introduced into the PB-Hyg-Dest plasmid using Gateway technology (Thermo Fisher Scientific). The PB-Hyg-Dest-mCherry-PAR-6B or the PB-Hyg-Dest-LifeAct-Ruby plasmid was co-transfected with the piggyBac plasmid using Lipofectamine 3000 to generate Hygromycin B resistant stable cell lines ...
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bioRxiv - Neuroscience 2022Quote: ... Plasmid DNA from the overnight cultures was harvested via extraction with the PureLinkTM HiPure Plasmid Filter Midiprep kit (Thermo Fisher Scientific), and insert sequence was confirmed by Sanger sequencing at the on-site DNA Sequencing Facility (Dept ...
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bioRxiv - Biochemistry 2022Quote: ... The constructs were transformed into competent DH5α (E. coli) cells and plasmids were extracted using GeneJET plasmid miniprep kit (Thermo Scientific) to verify sequence integrity by sequencing (Eurofins Genomics) ...
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bioRxiv - Neuroscience 2022Quote: ... we cloned sequences encoding each BoNT light chain (from plasmids shared by Matt Kennedy, University of Colorado, USA) into the gateway vector donor plasmid (ThermoFisher, # K240020). We then transferred these sequences into the final pUASt destination vector from Carnegie Drosophila Gateway Vector Collection using the Gateway reaction kit (Thermofisher ...
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bioRxiv - Cancer Biology 2022Quote: ... target plasmid was transfected together with psPAX2 and pMD2.G packaging plasmids into 293T cells using lipofectamine 2000 transfection reagent (Invitrogen, 11668027). Media was changed 24h later and lentivirus supernatant was collected after an additional 24 h ...
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bioRxiv - Molecular Biology 2021Quote: ... BHK-21 cells were transfected 24 h prior to pseudotype inoculation with either empty plasmid or ACE2 expression plasmid (0.1 μg/well) using Lipofectamine LTX (Thermo Fisher Scientific). In order to study the antiviral activity of Camostat mesylate ...
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bioRxiv - Cell Biology 2019Quote: Stable cell lines were generated by co-transfecting the plasmid of interest together with a plasmid carrying the resistance gene for hygromycin with Cellfectin II (Life Technologies), and the selection process was started 2 d after transfection by supplementing the media with 250 μg/ml Hygromycin B.
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bioRxiv - Developmental Biology 2021Quote: ... a transposase expressing plasmid and a TOL2 plasmid expressing mCherry (as a transfection control) following the Lipofectamine 2000 protocol (Life Technologies). 48 hours post transfection ...
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bioRxiv - Cancer Biology 2019Quote: Lentiviral vector system includes the pLEX-MCS plasmid and the PMD2G and psPAX2 packaging plasmids (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Genetics 2020Quote: ... HEK293T cells were seeded on 48-well plates and transfected with SlugABEmax/SlugBE4max plasmid (300 ng) and gRNA plasmid (200 ng) using 1 ul of Lipofectamine 2000 (Life Technologies) in Opti-MEM(Gibco ...
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bioRxiv - Genetics 2020Quote: ... cells were seeded on 48-well plates and transfected with Cas9 plasmid (300 ng) and gRNA plasmid (200 ng) using 1 ul of Lipofectamine 2000 (Life Technologies) in Opti-MEM according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: The inducible XIST constructs within the HT1080 2-3-0.5a cells were modified by transiently transfecting two unique gRNA plasmids (pSPgRNA) and a Cas9 plasmid providing transient puromycin resistance (pSpCas9(BB)-2A-Puro) using Lipofectamine 3000 (ThermoFisher #L3000008). The day before the transfection the HT1080 cells were split into 24 well plates ...
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bioRxiv - Immunology 2020Quote: ... The 3T3 cells were transfected with Fsp I linearized CD40L plasmid (a kind gift from G. Freeman21,22) and Pvu I linearized pcDNA3-Neomycin plasmid) using Lipofectamine 2000 Reagent (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were co-transfected with sleeping beauty transposon plasmid pSBi-Hyg-BFP (hTMEM120A cDNA) and sleeping beauty transposase plasmid pCMV (CAT) T7-SB100 with Lipofectamine2000 (Life Technologies). Three days after transfection ...
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bioRxiv - Genetics 2022Quote: ... Plasmids and packaging plasmids psPAX2 and pMD.2G were co-transfected into cells using Lipofectamine™ 3000 Transfection Reagent (Invitrogen, L3000015). The medium was changed after 12 hour incubation ...
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bioRxiv - Neuroscience 2022Quote: ... and plasmids were purified 18 h after the inoculation with a 96-well plasmid purification kit (PureLink Pro, Thermo Fisher Scientific) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... Approximately two million colonies were scraped from plates and pooled and plasmid DNA was purified using PureLink® HiPure plasmid filter Midiprep kit (Invitrogen). The resulting plasmid library was transformed into TRy1392 pdr5∆ yeast strain ...
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bioRxiv - Microbiology 2024Quote: ... transfections were performed by introducing 20 μg of targeting plasmid and 60 μg of linearised repair template (purified using Pure-Link™ HiPure Plasmid Midiprep Kit, Invitrogen) into ∽108 Percoll-enriched schizonts by electroporation using an Amaxa 4D Nucleofector X (Lonza) ...
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bioRxiv - Developmental Biology 2024Quote: ... 293 cells were transfected with plasmids encoding His-tagged recombinant proteins as described in the Plasmids section using lipofectamine 2000 (Invitrogen, #11668019) following the manufacturer’s instructions (four 100 mm plates each plasmid) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1.5 μg dual sgRNA plasmids or 1 μg sgRNA plasmids and 1 μg homologous recombination donor using Neon™ NxT Electroporation System (Invitrogen). After 48 hr ...
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bioRxiv - Immunology 2024Quote: ... 18 μg of pMDLg/p.RRE Gag/Pol plasmid and 18 μg of pRSV.Rev plasmid alongside 15 μg of transfer vector plasmid encoding for CAR of interest using Lipofectamine 2000 (Thermo Fisher Scientific) and Opti-MEM (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 µg of each plasmid (gagpol, vsv-g, and transfer plasmid) were combined with 2 ml Opti-MEM I Reduced Serum Medium (Gibco 31985070) and 90 µl MirusBio TransIT-LT1 Transfection Reagent (Mirus MIR2306 ...
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bioRxiv - Biophysics 2024Quote: ... HEK293T cells were co-transfected with 200 ng pCAGEN plasmid for TDP-43 and 1000 ng pCAGEN plasmid for the other protein using Lipofectamine 3000 Transfection Reagent (Invitrogen, L3000001) in 12-well plates ...
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bioRxiv - Immunology 2024Quote: ... pMDLg/p.RRE (18 μg) packaging plasmids and 15 μg of expression plasmids were mixed and transfected into 293T cells using Lipofectamine 3000 (Invitrogen #L3000150) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: The HEK293T packaging cells were transfected with OSA-expressing plasmids with lentiviral packaging plasmids using Lipofectamine Reagent 2000(Thermo Fisher Scientific). After 48 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... the SunTag plasmids and HMGB1-BFP (2 dishes) or Smad3-BFP plasmid (2 dishes) were simultaneously transfected with Lipo3000 transfection reagent (ThermoFisher Scientific). One dish of cells transfected with HMGB1-BFP ...
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bioRxiv - Molecular Biology 2023Quote: Extrachromosomal arrays were introduced by injection of 10 ng/μl plasmid DNA purified using the PureLink™ Quick Plasmid Miniprep Kit (Invitrogen) into the gonad of 1 DOA IR1284 (zcIs14[myo-3p::mtGFP] ...
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bioRxiv - Microbiology 2023Quote: ... The following day each well was co-transfected with 1 µg of replicon-encoding plasmid and 3 µg of C-prM-E-encoding plasmid using Lipofectamine 3000 (Cat# L3000-015; ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... were co-transfected with plasmids encoding GFP-nsP3 fusions and the integrase-encoding plasmid using Lipofectamine™ 3000 according to the manufacturer’s recommendations (ThermoFisher Scientific). Clones of blasticidin-resistant ...
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bioRxiv - Genomics 2023Quote: ... cells at 80–90% confluency were co-transfected with 0.25 µg of single-sgRNA (or 4sgRNA) plasmids and 0.25 µg of dCas9-VPR plasmids using Lipofectamine 3000 (Thermo Fisher Scientific). Three days post-transfection ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 5 ml of the culture was pelleted by centrifugation and plasmid DNA was isolated using a GeneJET Plasmid Miniprep Kit (Thermo Scientific). The plasmid sequence was verified using BigDye 3.1 sequencing chemistry and an ABI3130 Genetic Analyzer ...
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bioRxiv - Molecular Biology 2023Quote: ... was used at a 3:1 ratio with 1 µg total plasmid (500 ng nLuc plasmid + 500 ng pGL4.13) in 100 µL in Opti-MEM (Thermo Fisher # 31985062). For 96-well plates ...