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Citations for Thermo Fisher :
2501 - 2550 of 10000+ citations for 5 Nitro 1H benzo de isoquinoline 1 3 2H dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... at 37 °C with 5 % CO2 with 1 % penicillin/streptomycin (PS, 5000 U.mL-1 and 5000 μg.mL-1respectively; Life Technologies) and supplemented with 1% non-essential amino acids (Life Technologies).
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bioRxiv - Cell Biology 2021Quote: ... the stromal layer was washed with PBS and was subject to mild trypsinisation with 1:5 dilution of 0.25% Trypsin 1 mM EDTA (Thermofisher) to remove attached residual leukaemic cells before completely detaching stromal cells with 0.25% Trypsin-EDTA.
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bioRxiv - Systems Biology 2021Quote: ... Dried lipid fractions (~5 mg) were dissolved (isopropanol/dichloromethane 1:1, 300 μL) for accurate mass LC-MS (ThermoFisher Q exactive with Dionex Ultimate 3000 sample handler and Waters acquity UPLC BEH C18 with 1·7 μm particle size LC column ...
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bioRxiv - Genetics 2021Quote: ... PCR reactions contained 1 μl of 1:10 diluted lysate and 5 μl premixed AmpliTaq-Gold (0.3U, Life Technologies) with 0.26 μM of target-specific- and an additional fluorescent forward primer ...
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bioRxiv - Biochemistry 2020Quote: SHSY5Y cells were obtained from the Duke University Cell Culture Facility and were maintained at 37 °C with 5% CO2 in DMEM:F12 (1:1; Gibco) supplemented with 10% FBS (Sigma Aldrich ...
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bioRxiv - Pathology 2022Quote: ... resulting from UA were determined via a membrane-permeant JC-1 dye (5, 50, 6, 60 -tetrachloro-1, 10,3,30 -tetraethylbenzimidazol-carbocyanine iodide, Molecular Probes). JC-1 dye accumulates in mitochondria in a potential-dependent manner ...
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bioRxiv - Physiology 2021Quote: ... Frozen tissues were mechanically homogenized with 1 stainless steel bead (5 mm) in 1 ml Trizol (Thermo Fisher Scientific) by shaking for 50 s at 30 Hz (TissueLyser ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 min) and resuspended in 1 ml PBS containing 8 µl UltraPure BSA (50mg ml−1; AM2616, ThermoFisher Scientific) and filtered over Flowmi 40µm cell strainers (VWR ...
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bioRxiv - Genomics 2021Quote: ... nuclei were incubated for 1 hour in a solution containing 5% FBS and 1:500 primary antibody (H3K9me3; Invitrogen rabbit polyclonal 49-1008 or H3K9Ac ...
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bioRxiv - Cell Biology 2020Quote: ... All cells were maintained in 1:1 Ham’s F12 Dulbecco’s modified Eagle’s medium (FDV) containing 5% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 5ml/500ml 100X penicillin-streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were incubated with secondary antibodies conjugated to HRP at 1:20,000 in 5% milk/1x TBST for 1 hour (goat anti-mouse-HRP, 32430; ThermoFisher Scientific and goat anti-rabbit-HRP ...
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bioRxiv - Immunology 2021Quote: ... were diluted in two-fold dilution series from 1:5 to 1:5120 in Eagle’s Minimum Essential Medium (Gibco) with 5% FCS (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... primary antibody diluted in 5% NGS in PBS (β-catenin 1:200, ProteinTech, rhodamine-phalloidin 1:200, Molecular Probes) for 36 hours at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies were used at a ratio of 1:500 in 5% GS/1% BSA/0.2% PBST (anti-rabbit-Alexa-Fluor-555, Invitrogen A27039 ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 5% FBS and antibiotics (penicillin 100 U ml−1, streptomycin 100 U ml−1 (pen/strep) (Gibco), 10mM glutamax (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... 5′-labelled Aar RNA (4 nM) was incubated for 1 h at 37°C with 1× structure buffer (Ambion), 1 µg yeast RNA and putative mRNA interaction partners at the indicated concentrations (0 nM ...
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bioRxiv - Systems Biology 2024Quote: ... 1 μL of samples and 5 μL of standards were diluted into 95 μL of 1× SYBR green (Invitrogen) in TE buffer and mixed by pipette ...
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bioRxiv - Systems Biology 2024Quote: ... 1 μL of samples and 5 μL of standards were diluted into 95 μL of 1× SYBR green (Invitrogen) in TE buffer and mixed by pipette ...
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bioRxiv - Neuroscience 2024Quote: ... and the supernatants were collected and diluted at 1:5 or 1:10 with ice-cold Dulbecco’s PBS (Gibco). The supernatants were acidified to pH 2.6 and then neutralized to pH 7.6 ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclei were resuspended in 5% (w/v) BSA/PBST containing 1 µg µL−1 DAPI (Thermo Fisher Scientific, 62248). DAPI/NeuN-positive neuronal nuclei were sorted using a SONY SH800 flow cytometer.
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TSG101 Associates with PARP1 and is Essential for PARylation and DNA Damage-induced NF-κB ActivationbioRxiv - Molecular Biology 2021Quote: ... Incubation with primary antibodies (PARP1, Thermo Fisher, Cat#MA 3-950, TSG101, Thermo Fisher, Cat#MA 1-23296, both diluted 1:200) was done overnight at 4°C.
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bioRxiv - Biochemistry 2020Quote: ... The wells were washed with 1% BSA/LBB and incubated for 1 hour with L9393 (1:5,000 dilution) in 3% BSA/LBB followed by incubation with Horseradish Peroxidase (HRP)-conjugated anti-rabbit IgG (Invitrogen, 1:5,000 dilution) in 3% BSA/LBB for 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... On the second day samples were washed (0.3% PBSTx, 3×1 hr) and subsequently incubated with the secondary antibody (Alexa-labelled GAM555 plus, Thermo Scientific, 1:1,000) overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Testes were homogenized manually followed by dropping 1 μl of suspension into 30 μl of hypotonic solution (1/3 of 1× PBS) and then dropped onto SuperFrost® slides (Menzel Gläser; Thermo Fisher Scientific). The samples were fixed in 400 μl of 2% PFA for 4 min ...
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bioRxiv - Neuroscience 2020Quote: ... goat anti-β-ACTIN (AB0145-200, SICGEN, 1:1000 or 3 ug/mL) and rabbit anti-GFP (A-6455, Invitrogen, 1:1000). The secondary antibodies used were Sheep IgG (H&L ...
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bioRxiv - Genomics 2021Quote: ... and then blocked for 1 hour with 1 ml of blocking buffer (wash buffer containing 3% fatty acid free BSA (Thermo Fisher, 126609)) ...
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bioRxiv - Cell Biology 2020Quote: The following antibodies were used: anti-cleaved-caspase 3 (1:200; CST, #9664s) and secondary Alexa Fluor 647 anti-rabbit (1:500; Invitrogen, #A-20991). Reagents including EN6 (Selleck ...
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bioRxiv - Genomics 2022Quote: ... HEK293FT cells were replated at a 1:3 dilution one day post-transfection into media supplemented with 1 μg/mL final concentration puromycin (Thermo Fisher Scientific). HEPG2 cells (American Type Culture Collection (ATCC – HB8065 ...
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bioRxiv - Developmental Biology 2022Quote: ... Human naive PSCs were passaged as single cells every 4 days at split ratio 1:3 or 1:4 following dissociation with TrypLE (Gibco 12563-029) for 10 minutes (min ...
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bioRxiv - Physiology 2021Quote: ... The wells were washed with 1% BSA/LBB and incubated for one hour with L9393 (1:5,000 dilution) in 3% BSA/LBB followed by incubation with HRP-conjugated anti-rabbit IgG (Invitrogen, 1:5,000 dilution) in 3% BSA/LBB for 30 minutes ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... with 3 µg plasmid DNA and 1 × 106 cells in 100 µl Opti-MEM (1×) Reduced Serum Medium (Gibco® ThermoFisher Scientific). The electroporated cells were cultured in a non-selective complete medium in a 100 mm × 20 mm cell culture dish (Falcon ...
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bioRxiv - Evolutionary Biology 2021Quote: For sucrose preference assays, experimental substrates were 1% agarose and contained 1% ethanol and increasing concentrations (200mM, 400mM or 600mM) of sucrose (ThermoFisher #S5-3); control substrates were 1% agarose and contained 1% ethanol.
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bioRxiv - Bioengineering 2021Quote: ... HEK293FT cells were replated at a 1:3 dilution one day post-transfection into media supplemented with 1 µg/mL final concentration puromycin (Thermo Fisher Scientific). HEPG2 cells (American Type Culture Collection (ATCC – HB8065 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed in 0.1% Triton X-100 PBS for 3 × 10 min before incubation with either donkey anti-rabbit Alexa fluor 488 (Invitrogen, 1:1000) or donkey anti-mouse Alexa fluor 555 (Invitrogen ...
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bioRxiv - Pathology 2022Quote: ... and cell monolayers were overlaid with 3 mL containing a 1:1 mixture of 1.2% oxoid agar and DMEM (Gibco, Carlsbad, CA, USA) with 10% (vol/vol ...
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bioRxiv - Neuroscience 2023Quote: ... (3) positive staining for IBA1 (rabbit primary, Wako, 019-1974, 1:2000, donkey anti-rabbit 647 Life Technologies, A-31573, 1:1000) via 2D immunofluorescence imaging and (4 ...
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bioRxiv - Neuroscience 2024Quote: ... followed by culturing with the neural induction medium supplemented with 20 ng/ml human FGF-2).61 The passage was performed twice per week (1:2 or 1:3) using Accutase (Cat #A1110501, Thermo Fisher Scientific) by vigorously breaking pellets to remove neuronal cells ...
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bioRxiv - Pathology 2023Quote: ... and cell monolayers were overlaid with 3 mL containing a 1:1 mixture of 1.2% oxoid agar and DMEM (Gibco, Carlsbad, CA, USA) with 10% (vol/vol ...
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bioRxiv - Developmental Biology 2023Quote: ... Human naive PSCs were passaged as single cells every 4 days at split ratio 1:3 or 1:4 following dissociation with TrypLE (Gibco 12563-029) for 10 minutes at room temperature (RT) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were pelleted at 4000xg and pellets were washed with water (3 × 10 mL) and resuspended in 1 mL of water and 1 mL of TraceMetal Grade 70% HNO3 (Thermo Fisher Scientific). Acidified cell pellets were boiled at 90 °C for 1 hr and pelleted at 4000xg ...
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bioRxiv - Bioengineering 2024Quote: ... NK cells and target cells were mixed at an effector to target (E/T) ratio of 4:1 (SK-BR-3 cells) or 2:1 (K562 cells) and Sytox Green (Thermo Fisher Scientific) was added at 100 nM ...
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bioRxiv - Bioengineering 2024Quote: ... in 1X TBS adjusted to pH 7.6 for 1 hour at room temperature and rinsed 3 times for 1 hour in 0.1% Tween-20 (Thermo Fisher, Waltham, MA) in 1X TBS adjusted to pH 7.6 (TBS-T) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were passaged every 2-3 days with 1:4-1:6 ratio by incubation cells with 0.5 mM EDTA (Fisher Scientific MT-46034CI) for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... The monolayers were then overlaid with a 1:1 mixture of 2.5% Avicel® RC-591 microcrystalline cellulose and carboxymethylcellulose sodium (DuPont Nutrition & Biosciences, Wilmington, DE) and 2X Modified Eagle Medium (Temin’s modification, Gibco-Thermo Fisher Scientific) supplemented with 2X antibiotic - antimycotic ...
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bioRxiv - Molecular Biology 2021Quote: ... 10% acetic acid and 0.5% Coomassie G-250) and de-stained (10% methanol; 10% acetic acid) or silver stained using the Silver Quest kit (Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2022Quote: ... masses were verified via matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (Applied Biosystems - Voyager-DE™ PRO) (Figure S1 ...
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bioRxiv - Molecular Biology 2024Quote: ... The purity and quantity of the isolated total RNA were measured using a Nanodrop™ 2000 spectrophotometer (Thermo Fisher Scientific, Wilmington, DE).
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bioRxiv - Cancer Biology 2024Quote: ... Density of harvested fractions was measured based on iodixanol absorbance at 250 nm using a NanoDrop spectrophotometer (Thermo Fisher Scientific, Wilmington, DE). Vesicle harvests were stored at − 80°C.
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bioRxiv - Microbiology 2023Quote: Cells were grown in 16×25 mm glass tubes with 3 ml of Hv-Cab (de Silva et al., 2021) at 42°C under constant agitation with the inducers D-xylose (Thermo Scientific Chemicals), L-arabinose (Thermo Scientific Chemicals) ...
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bioRxiv - Immunology 2023Quote: ... Nucleic acids concentration and absorbance ratio at 260/280 nm were assessed with the Nanodrop One spectrophotometer (Thermo Scientific, Wilmington, DE, USA) at an optical density of 260 nm (OD260).