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Citations for Thermo Fisher :
2301 - 2350 of 10000+ citations for 5 Nitro 1H benzo de isoquinoline 1 3 2H dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: The FM™ 1-43 (N-(3-Triethylammoniumpropyl)-4-(4-(Dibutylamino) Styryl) Pyridinium Dibromide) (Invitrogen) membrane probe was used to identify actively firing neurons and to investigate mechanisms of activity-dependent vesicle cycling ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed with diluted trypsin solution (1:3, in 1X DPBS [Gibco, 14040-091]), and allowed to grow further or processed for experiments.
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bioRxiv - Immunology 2024Quote: ... were produced in HEK293F by transfecting with PEI (3:1 ratio) or Expi-293F (ThermoFisher) cells following the manufacturer’s recommended transfection protocol ...
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bioRxiv - Neuroscience 2024Quote: ... and TO-PRO-3 was used to label cell nuclei (1 µM, Thermo Fisher Scientific). Images were acquired with a confocal microscope (Leica SP5 ...
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bioRxiv - Developmental Biology 2024Quote: ... cerebella were washed 3 x 1 h in 50% OCT (Fisher Scientific, 23-730-571) before embedding in 100% OCT ...
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bioRxiv - Immunology 2020Quote: ... purified B cells were incubated at 6 × 106 cells ml-1 in 1 mL of PBS with 1 uL of 5 mM Cell Trace Violet (CTV) dye (Thermo Scientific). The CTV-stained B cells were washed and activated as detailed above and subjected to flow cytometry analyses 4 days after activation.
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bioRxiv - Microbiology 2023Quote: ... Transfection was performed with 5 µg of pLKO.1 puro pri-mir-1-1 vector using Lipofectamine 3000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... and shRNA plasmids or pCDH plasmids by PEI-40000 with the ratio of 5: 1: 5 in opti-MEM (Gibco, Thermo Fisher Scientific). Virions were collected after 48 h after transfection ...
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bioRxiv - Cell Biology 2020Quote: ... organoids (after 1 week of culture) were incubated (37°C, 5% CO2, 1 h) with 10 µM EdU (5-ethynyl-2’-deoxyuridine, Thermo Fisher Scientific), a nucleoside analog of thymidine incorporated into DNA during active DNA synthesis ...
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bioRxiv - Molecular Biology 2022Quote: ... and incubating at 37°C in a 5% CO2 humidified incubator in DFGM supplemented with 5 ng mL−1 TGF-β1 (Thermo Fisher Scientific) and 100 μg mL−1 ascorbic acid ...
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bioRxiv - Microbiology 2024Quote: ... each culture was diluted 1:1000 in BHI + 5% yeast and 10μl were streaked on a BHI + 5% yeast agar (Thermo Fisher Scientific, CM1136) plate ...
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bioRxiv - Immunology 2024Quote: Analysis of cytokines and chemokines from sera and lungs homogenates collected 5 dpi and 5 dpc were conducted using the ProcartaPlex TM Mouse Cytokine & Chemokine Convenience Panel 1 26-plex (Thermo Fisher Scientific) following the manufacturer’s specifications ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were again washed 3x with 1 mL DPBS for 5 min each followed by nuclear labeling for 5 min with 1 mL 300 nM DAPI dissolved in DPBS (Thermo Fisher Scientific) and a 5-min DPBS wash ...
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bioRxiv - Molecular Biology 2021Quote: ... The quality of the RNA was determined using a Nanodrop spectrophotometer with A260/230 ≥1.6 and A260/A280 set at ≥1.8 (Thermo Scientific, Wilmington, DE). The cDNA was generated from mRNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems by Life Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA samples were used for global gene expression profiling on human Clariom S Assay microarrays (Thermo Fisher Scientific, Wilmington, DE, USA), which include 24351 genes ...
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bioRxiv - Cancer Biology 2020Quote: Following confirmation of the quality and quantity of extracted total RNA samples with a Nanodrop 2000 (Thermo Scientific, Wilmington, DE, USA), cDNA was synthesized using an iScript cDNA Synthesis Kit (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... then analyzed using HPLC/MS/MS (Shimadzu SCL10Avp-Wilmington, DE, USA; API 3000 Applied Biosystems/MDS SCIEX, Foster City, CA, USA) as previously described (Leishman et al. ...
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bioRxiv - Microbiology 2021Quote: ... The quantity and quality of the obtained DNA was checked by ND1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA) and Qubit 2.0 fluorometer (ThermoFisher Scientific, USA). DNA samples were stored at -20°C until further use.
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bioRxiv - Cancer Biology 2022Quote: ... The peptides were eluted from the pre-column onto an Easy-spray (25 cm x 75 μm; Thermo Fisher Scientific, DE) column into the Orbitrap Exploris 480 mass spectrometer ...
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bioRxiv - Biochemistry 2020Quote: ... Injected peptide samples were on-line de-salted/concentrated using an Acclaim PepMap C18 trap column (75 μm × 2 cm, Thermo Scientific) for 2 min at 5uL/min using 2% MeCN + 0.2% FA ...
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bioRxiv - Microbiology 2021Quote: ... The quality of DNA and total RNA was assessed by NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific, Inc., Wilmington, DE, USA) and the ratio of absorbance at 260 nm and 280 nm was around 1.8-2.0 ...
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bioRxiv - Microbiology 2021Quote: ... DNA concentrations in all samples and negative controls were quantified using a NanoDrop ND-1000 (Thermo Scientific, Inc., Wilmington, DE, USA). The DNA extracts were stored at −80°C until sequenced.
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bioRxiv - Evolutionary Biology 2021Quote: ... The quality and quantity of DNA for all samples were evaluated using a NanoDrop 1000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA). Mate-paired and paired-end libraries (200 bp and 2 x 100 bp ...
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bioRxiv - Microbiology 2020Quote: ... The monolayers were then overlaid with a 1:1 mixture of 2.5% Avicel® RC-591 microcrystalline cellulose and carboxymethylcellulose sodium (DuPont Nutrition & Biosciences, Wilmington, DE) and 2X Modified Eagle Medium (Temin’s modification, Gibco-Thermo Fisher Scientific) supplemented with 2X antibiotic - antimycotic ...
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bioRxiv - Immunology 2021Quote: ... The 10 larvae from each collection were placed into a 1.5 mL microcentrifuge tube (Thermo Fisher Scientific Inc., Wilmington, DE, USA) and immediately flash frozen in an isopropanol/dry ice mixture ...
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bioRxiv - Genomics 2021Quote: ... DNA purity was checked using a NanoDrop reader (ND-2000, NanoDrop Technologies, Wilmington, DE, USA) and precision-quantified using Qubit HS reagents (Life Technologies). The DNA template concentration was adjusted to 5ng prior for use in touchdown PCR reactions ...
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bioRxiv - Molecular Biology 2023Quote: Separation of peptides produced by AspN digestion was performed on a ICS3500 nano-UHPLC system (Thermo Fisher Scientific/Dionex, Germering, DE)) employing a 75 μm x 150 mm Easy spray column packed with C-18 reverse phase (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... cells were resuspended in PBS and the fluorescence was determined with a Qubit 4 fluorometer (Thermo Fisher Scientific, Wilmington, DE, USA) with excitation at 470 nm and emission at 510 – 580 nm ...
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bioRxiv - Plant Biology 2024Quote: ... All DNA samples were quality checked and the concentration was quantified by NanoDrop 2000 spectrophotometers (Thermo Fisher Scientific, Wilmington, DE, USA). Bacterial 16S rRNA gene fragments (the hypervariable region V3−V4 ...
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bioRxiv - Molecular Biology 2024Quote: ... The concentration and quality of extracted RNA from the lung tissues were measured using NanoDrop® One (Thermo Scientific, Wilmington, DE) and reverse-transcribed into cDNA using Verso cDNA Synthesis Kit (ThermoFisher) ...
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bioRxiv - Biophysics 2022Quote: ... Their monomeric molar masses are known from sequence and were verified by mass-spectrometry on an AB Voyager-DE PRO MALDI-TOF instrument (Applied Biosystems now ThermoFisher). Stoichiometries are assumed to be comprised of known molar masses of SKp and CaM ...
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bioRxiv - Cell Biology 2023Quote: ... trypsin de-activation was achieved using the hiPSC-CM maintenance media supplemented with 10% FBS (Fetal Bovine Serum, Thermo Fisher Scientific), and cells were pelleted by centrifugation at 200 g for 5 minutes ...
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bioRxiv - Genomics 2024Quote: ... After the de-staining in-gel trypsin digestion was performed and samples were loaded into Mass spectrometry using Q Exactive (Thermo Scientific) interfaced with nanoflow LC system (Easy nLC 1200 ...
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bioRxiv - Molecular Biology 2024Quote: ... hESCs were differentiated to APS and DE with a two-day protocol using PSC Definitive Endoderm Induction Kit (Thermo Fisher, A3062601) or home-made media 36 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... grown to 70% confluency in a Dulbecco’s Modified Eagle Medium mixture (cytiva HyClone DMEM, Wilmington, DE, USA; 10% Fetal Bovine Serum, Fisher Scientific; 100 U/mL penicillin and streptomycin, Gibco; and 1x GlutaMAX ...
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bioRxiv - Genetics 2022Quote: ... 20 mL of 3 mM of 3-deoxyadenosine (cordycepin; Thermo Fisher Scientific) was added to the buffer to obtain a final concentration of 0.6 mM (150 mg/L) ...
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bioRxiv - Cell Biology 2022Quote: ... seeded on t25 flasks at 37°C 5% CO2 in DMEM/F12 (1:1) (ThermoFisher Scientific, Cramlington, UK) with 10%FBS ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were cultured at 37C and 5% CO2 either in 1:1 mix of DMEM GlutaMAX (Gibco, 31966047) and F-12 media (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... and pYK-JRCSF HIV-1 expression plasmid in a 5:1 mass ratio with Lipofectamine 2000 (Life Technologies) according to the manufacturer’s manual.
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Disruption of the TCA cycle reveals an ATF4-dependent integration of redox and amino acid metabolismbioRxiv - Biochemistry 2021Quote: ... Samples were loaded onto 4-12% Bis-Tris Bolt™ gradient gels and run at 160V constant for 1h in Bolt™ MES 1X running buffer (Thermo Scientific). Dry transfer of the proteins onto a nitrocellulose membrane was done using iBLOT2 (Thermo Scientific ...
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bioRxiv - Physiology 2023Quote: ... at 37°C for 1h and subsequently with donkey anti-rabbit IgG (H+L) Highly Cross-Adsorbed Secondary AntibodyAlexa-647 (ThermoFisher Scientific #A-31573) at 37°C for 1h ...
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bioRxiv - Molecular Biology 2024Quote: ... Proper secondary antibodies were used for 1h at room temperature before visualised the membranes with Pierce™ ECL Western Blotting Substrate (Thermo Scientific, 10005943) or SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Scientific ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... OCT-embedded penis sections were permeabilized in PBS with 0.3% Triton X-100 at room temperature for 10 min following by 1h incubation with blocking buffer containing 10% normal goat serum (Life Technologies, 16210-072) and 0.1% Tween20 in PBS at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the slides were blocked for 1h at RT with 1XPBS + 10% normal goat serum (NGS, 60.0 mg/mL, Thermo Fisher Scientific, Ghent, Belgium) and 1%FBS and washed again twice with 1XPBS for 5’ each ...
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bioRxiv - Neuroscience 2024Quote: ... cells were washed 3x for 5 min with PBS and incubated for 1h at RT with Alexa Fluor® conjugated secondary antibodies (Table S3) and nuclei stain Hoechst 33342 (Invitrogen cat.no. 62249). After 3x washes for 5 min with PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... the sections were washed once with 1x PBS and incubated for 1h with tyramide working solution (Alexa Fluor™ 594 Tyramide SuperBoost™ Kit, goat anti-rabbit IgG, Invitrogen, ThermoFisher scientific) by following the manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... the sections were washed once with 1x PBS and incubated for 1h with tyramide working solution (Alexa Fluor™ 555 Tyramide SuperBoost™ Kit, goat anti-rabbit IgG, Invitrogen, ThermoFisher scientific) by following the manufacturer instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Culture medium was refreshed every 2–3 days and organoids were passaged 1:2–1:6 every 7–21 days using TrypLE Express (Thermo Fisher). For co-culturing ...
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bioRxiv - Molecular Biology 2020Quote: Single- and double-stranded oligonucleotide substrates were diluted in TBST with 0.1% BSA and 3 mM magnesium chloride to 1 μM final concentrations Previously mentioned enzymes and RNase A (EN0531, Thermo Fisher) were used at a 1:200 final dilution and incubated with indicated nucleic acid substrates for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were rinsed 3 times in PBS and incubated for 1 h at room temperature with corresponding secondary antibodies (1:500, Life Technologies). Sections were washed three times with PBS and incubated with DAPI for 5 min (1:5,000 in PBS ...