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Citations for Thermo Fisher :
2751 - 2800 of 10000+ citations for 5 Nitro 1H benzo de isoquinoline 1 3 2H dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... 3 mM MgCl2 (Ambion/Invitrogen), 0.1% Nonidet-P40 substitute (Research Products International) ...
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bioRxiv - Genetics 2023Quote: ... 3 mM MgCl2 (Ambion/Invitrogen), 0.1% Nonidet-P40 substitute (Research Products International) ...
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bioRxiv - Cancer Biology 2022Quote: ... and Qubit 3 fluorometer (Invitrogen). Sequencing was performed by the Genomic Technologies Core Facility at the University of Manchester ...
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bioRxiv - Genetics 2023Quote: ... 3 mM MgCl2 (Ambion/Invitrogen), 0.1% Nonidet-P40 substitute (Research Products International) ...
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bioRxiv - Bioengineering 2024Quote: ... Step 3: Cavicid (Fisher Scientific), Step 4 ...
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bioRxiv - Cell Biology 2024Quote: ... Flt-3 (A2F10, Thermofisher Scientific), CD48 (HM48-1 ...
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bioRxiv - Microbiology 2024Quote: ... 3 μl Lipofectamine RNAiMAX (Invitrogen) was mixed with 100 μl Opti-MEM and incubated at room temperature for 1 min ...
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bioRxiv - Molecular Biology 2024Quote: ... 3% bovine serum albumin (ThermoFisher) and 5% normal goat serum (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... 3 µg/ml puromycin (Gibco) was added 48 hours post-transduction ...
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bioRxiv - Neuroscience 2024Quote: ... pHrodo (Invitrogen, P36600, 3 μM) dissolved in DMSO was added to the synaptosomes and incubated for 45 minutes at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... DiIC12(3) (Thermo Fisher Scientific), was added to a final concentration of 5 μg/mL in GPMV buffer and incubated for 15 minutes at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... 3 µm column (Thermo Scientific) and a 6210 electrochemical cell (ESA ...
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bioRxiv - Microbiology 2024Quote: ... 3 µg/ml Puromycin (Gibco) was added 48 hours after transduction before knockdown analysed >8 days post transduction via qPCR and western blot if appropriate32.
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bioRxiv - Cancer Biology 2024Quote: ... and QuantStudio 3 (Thermo Fisher). All primers used here are listed in Supplementary Table 3 ...
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bioRxiv - Cell Biology 2024Quote: ... with Quantstudio 3 (Applied biosystems). microRNA levels were normalized by as the combination of endogenous control as internal standard ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and they were sequenced at the “Service de Systématique Moléculaire” (UMS CNRS 2700, MNHN, Paris, France) using the Ion Torrent Personal Genome Machine (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Molecular Biology 2021Quote: ... Mass spectrometry was performed with an Applied Biosystems Voyager DE-Pro matrix-assisted laser desorption/ionization mass spectrometer from Life Technologies (Carlsbad, CA) in the Biophysics Instrumentation Facility at University of Wisconsin-Madison.
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bioRxiv - Microbiology 2021Quote: ... Liquid cultures were grown in brain heart infusion media (BHI) supplemented with 10% defibrillated and de-complemented fetal bovine serum (Invitrogen, Carlsbad, CA, USA) with constant shaking (180 rpm) ...
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bioRxiv - Neuroscience 2020Quote: ... containing the amygdala were cut in ice-cold slicing ACSF with a sapphire blade (Delaware Diamond Knives, Wilmington, DE, USA) on a vibrating microtome (Microm HM650V, Thermo Scientific, Dreieich, Germany). Slices were collected in a custom-built interface chamber with recording ACSF containing (in mM) ...
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bioRxiv - Physiology 2020Quote: ... The RNA samples were checked for purity (A260/A280, A260/A230) and concentration using a NanoDrop One Spectrophotometer (ThermoFisher Scientific, Wilmington, DE, USA). The integrity of the RNA was assessed by electrophoresis on a 1% agarose gel ...
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bioRxiv - Cell Biology 2020Quote: ... RNA was resuspended in molecular-grade H2O and quantified by measuring the optical density (230, 260, and 280 nm) with a Nanodrop 1000 Spectrophotometer (ThermoFisher Scientific, Wilmington, DE). Nascent (EU-labeled ...
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bioRxiv - Pathology 2022Quote: ... and non-esterified fatty acids (NEFA) were assessed by commercial assay kits (Jiancheng, Nanjing, China) and monitored with a NanoDrop 2000 Spectrophotometer (Thermo Scientific, Wilmington, DE).
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bioRxiv - Plant Biology 2021Quote: ... DNA quality was checked by agarose gel electrophoresis and quantified with a NanoDrop spectrophotometer ND-1000 version (Thermo Fisher Scientific, Wilmington, DE, USA). For GBS library construction ...
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bioRxiv - Microbiology 2020Quote: ... DNA samples were eluted in 50-100 µl DES water and DNA concentrations were determined using the Qubit™ dsDNA HS Assay Kit (Invitrogen, life technologies) on Qubit®2.0 (Life Technologies).
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bioRxiv - Immunology 2022Quote: ... Reverse transcription of mRNA into complementary (c)DNA was performed using the Veriti 96-well Thermal Cycler (Thermo Fisher Scientific, Wilmington, DE, USA). Dynabeads MyOne SILANE (10x Genomics ...
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bioRxiv - Neuroscience 2020Quote: ... Newark DE USA) with flanking attB1/B2 sites for subsequent Invitrogen BP Gateway recombinational cloning into pDONR221 entry vector (Thermofisher, Waltham MA USA). To produce mammalian expression constructs ...
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bioRxiv - Cell Biology 2020Quote: DNA was isolated from 10 mg of frozen native and from freshly decellularized bronchi using DNeasy blood and tissue Kit® (Invitrogen, Hilden, DE) as recommended by the manufacturer ...
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A tale of two transcriptomic responses in agricultural pests via host defenses and viral replicationbioRxiv - Genomics 2020Quote: ... and integrity of the total RNA was sequentially assessed using the A260/A280 values reported with a Nanodrop spectrophotometer (Thermo Scientific, Wilmington, DE), agarose gel electrophoresis ...
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bioRxiv - Cancer Biology 2021Quote: ... The peptide mass was characterized by matrix assisted laser desorption/ionization (MALDI) on a Voyager DE Pro (Applied Biosystems Inc., Foster City, CA). The pure Def1 conjugate was air oxidized slowly with continuous stirring for 36-48 hrs and monitored the formation of S-S folding using MS/MS ...
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bioRxiv - Cell Biology 2023Quote: ... The bacterial expression plasmid pET100D-TOPO-DES was generated by TOPO cloning according to the manufacturer’s instructions (Thermo Fisher Scientific, Waltham, MA, USA) and was previously described [27] (Supplements ...
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bioRxiv - Genomics 2023Quote: ... DNA purity was determined using a NanoDrop 1000 spectrophotometer (NanoDrop Technologies Inc., Wilmington, DE, USA) and quantified using the Qubit 2.0 Fluorometric Quantitation (Invitrogen, Life technologies, Sydney, N.S.W., Australia). The sex-reversal status was determined for B ...
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bioRxiv - Genomics 2023Quote: ... The disulfide-linked probes were de-stained by cleaving the disulfide bridge between the probe and its fluorophore with 50 mM TCEP (Thermo Fisher Scientific #363830100) in 2X saline-sodium citrate (SSC ...
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bioRxiv - Cell Biology 2023Quote: ... RNA quantity and quality were assessed by the ratio of absorbance at 260/280 nm using NanoDrop 2000 spectrophotometer (Thermofisher Scientific, DE, UK), and TapeStation (Agilent Technologies ...
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bioRxiv - Genomics 2024Quote: ... were extracted from leaves using improved CTAB method31 and evaluated using NanoDrop One spectrophotometer (NanoDrop Technologies, Wilmington, DE) and Qubit 3.0 Fluorometer (Life Technologies, Carlsbad, CA, USA). For the genome survey ...
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bioRxiv - Physiology 2023Quote: Peptides were injected onto 30 cm C18 columns with 1.8 μm beads (Sepax, Newark, DE, USA) on an Easy nLC-1200 HPLC (Thermo Fisher, Carlsbad, CA, USA) connected to a Fusion Lumos Tribrid mass spectrometer (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... Purity of extracted DNA samples was determined by absorbance (260/280 and 260/230 ratios) using a Nanodrop-1000 (ThermoFisher, Nandrop Wilmington, DE). The DNA extraction triplicates were PCR (using EUK1A and 516R primers ...
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bioRxiv - Cell Biology 2023Quote: ... Concentrations of proteins and peptides were calculated from the UV absorption at 280 nm using a Nanodrop spectrophotometer (Thermo Fisher Scientific, DE; USA).
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bioRxiv - Systems Biology 2022Quote: Slides are baked to de-paraffinize at 45°C for 20 minutes and are rehydrated in three washes of histological grade xylene (Fisher Scientific #X3S-4) followed by two washes each of ethanol (100% ...
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bioRxiv - Biophysics 2022Quote: ... Their monomeric molar masses are known from sequence and were verified by mass-spectrometry on an AB Voyager-DE PRO MALDI-TOF instrument (Applied Biosystems now ThermoFisher). Stoichiometries are assumed to be comprised of known molar masses of SKp and CaM ...
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bioRxiv - Bioengineering 2021Quote: ... 5 × 10−5 M 2-mercaptoethanol (Gibco, 31350-010), 1X Minimal Essential Medium (MEM ...
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bioRxiv - Bioengineering 2021Quote: ... 5 × 10-5 M 2-mercaptoethanol (Gibco, 31350-010), 1X Minimal Essential Medium (MEM ...
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bioRxiv - Bioengineering 2023Quote: ... stained with 5 ng/mL dichlorotriazinylaminofluorescein (5-DTAF, Invitrogen), and mounted in custom grips at a 10 mm gauge length ...
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bioRxiv - Cell Biology 2024Quote: ... 5% CO2 and 5% O2 in RPMI 1640 (Gibco) with 15% FBS (Gibco ...
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bioRxiv - Biochemistry 2020Quote: ... TFA and 1 μL of this dilution and 1μL of the undiluted peptides from bands 3 and 4 were injected onto the trapping column (Thermo Scientific, PepMap100, C18, 300 μm × 5 mm), using a partial loop injection ...
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bioRxiv - Cell Biology 2021Quote: Human LAMP2A was synthesized into pUC57 Plasmid with the sequence: (5’-CAAGTTTGTACAAAAAAGCAGGCTCTCGAGCAatgGTGTGCTTCCGCCTCTTCCCGGTTCCGGGCTCAGGGCTCGTTCTGGTCTGCCTAGTCCTGGGAGCTGTGCGGTCTTATGCATTGGAACTTAATTTGACAGATTCAGAAAATGCCACTTGCCTTTATGCAAAATGGCAGATGAATTTCACAGTACGCTATGAAACTACAAATAAAACTTATAAAACTGTAACCATTTCAGACCATGGCACTGTGACATATAATGGAAGCATTTGTGGGGATGATCAGAATGGTCCCAAAATAGCAGTGCAGTTCGGACCTGGCTTTTCCTGGATTGCGAATTTTACCAAGGCAGCATCTACTTATTCAATTGACAGCGTCTCATTTTCCTACAACACTGGTGATAACACAACATTTCCTGATGCTGAAGATAAAGGAATTCTTACTGTTGATGAACTTTTGGCCATCAGAATTCCATTGAATGACCTTTTTAGATGCAATAGTTTATCAACTTTGGAAAAGAATGATGTTGTCCAACACTACTGGGATGTTCTTGTACAAGCTTTTGTCCAAAATGGCACAGTGAGCACAAATGAGTTCCTGTGTGATAAAGACAAAACTTCAACAGTGGCACCCACCATACACACCACTGTGCCATCTCCTACTACAACACCTACTCCAAAGGAAAAACCAGAAGCTGGAACCTATTCAGTTAATAATGGCAATGATACTTGTCTGCTGGCTACCATGGGGCTGCAGCTGAACATCACTCAGGATAAGGTTGCTTCAGTTATTAACATCAACCCCAATACAACTCACTCCACAGGCAGCTGCCGTTCTCACACTGCTCTACTTAGACTCAATAGCAGCACCATTAAGTATCTAGACTTTGTCTTTGCTGTGAAAAATGAAAACCGATTTTATCTGAAGGAAGTGAACATCAGCATGTATTTGGTTAATGGCTCCGTTTTCAGCATTGCAAATAACAATCTCAGCTACTGGGATGCCCCCCTGGGAAGTTCTTATATGTGCAACAAAGAGCAGACTGTTTCAGTGTCTGGAGCATTTCAGATAAATACCTTTGATCTAAGGGTTCAGCCTTTCAATGTGACACAAGGAAAGTATTCTACAGCTCAAGACTGCAGTGCAGATGACGACAACTTCCTTGTGCCCATAGCGGTGGGAGCTGCCTTGGCAGGAGTACTTATTCTAGTGTTGCTGGCTTATTTTATTGGTCTCAAGCACCATCATGCTGGATATGAGCAATTTTAGGGTACCACCCAGCTTTCTTGTACAAAGTGGACGCGT-3’) Human LAMP2A was subcloned into pLenti6 (Thermo Scientific), a Gateway adapted vector using BP/LR Clonase II (Thermo Scientific) ...
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bioRxiv - Plant Biology 2021Quote: ... The supernatant was discarded and the pellet was resuspended in 5 mL liquid KNOP supplemented with 2% (w/v) sucrose (#S/8600/60, Fisher) and 100 μM 3’,5’-dimethoxy-4’-hydroxyacetophenone (acetosyringone) (#115540050, Acros Organics, dissolved in dimethyl sulfoxide (DMSO) (#D8418 ...
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bioRxiv - Systems Biology 2023Quote: ... and washed again in PBS (3 x 5 min at RT) before being mounted in Prolong Gold (Invitrogen by Thermo Fisher Scientific, Grand Island, NY, USA) and allowed to cure for 48 hours at RT before imaging ...
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bioRxiv - Synthetic Biology 2024Quote: ... cultures were plated on LB agar plates containing 60 μg/mL 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside (X-gal; Thermo Fisher Scientific catalogue no. R0402). Antibiotics in media for bacterial growth were used at the following working concentrations ...
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bioRxiv - Cell Biology 2024Quote: ... slides were washed in PBS 3×5 minutes and then stained with the secondary antibody Goat anti-rabbit AlexaFluor555 (Invitrogen #A21429, working concentration 2 µg/µl) or Goat anti-rat Alexa488 (Thermofisher #A-11006 ...
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bioRxiv - Developmental Biology 2024Quote: ... while the rest were re-plated onto three matrigel-coated 10 cm dishes (3 replicates / 3 million cells per plate) in N2/B27 media containing 3 µg/mL puromycin (Fisher Scientific; Cat. no. 501532829) to select for the pMK1334 sgRNA vector ...