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Citations for Thermo Fisher :
2401 - 2450 of 4608 citations for 6 HYDROXY CHRYSENE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... and blotted for 4 to 6 seconds at 4°C and 100% humidity using the Vitrobot system (ThermoFisher), before plunging immediately into liquid ethane for vitrification ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa cells were cultured to ∼60% confluency in 6-well plates and transfected with Lipofectamine 3000 (Invitrogen L3000) following directions provided by the manufacturer ...
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bioRxiv - Cell Biology 2022Quote: ... samples were incubated for 30 min at 37°C with the secondary antibody diluted in PBS/3% BSA/0.05% Tween 20 (see antibodies section) and 4’,6-diamidino-2-phenylindole (DAPI 0.125 mg/ml, Invitrogen) in PBS/3% BSA/0.05% Tween 20 ...
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bioRxiv - Neuroscience 2022Quote: ... the cerebral cortexes from 2-3 P3-P5 C57BL/6 mice were collected in ice cold HBSS (Invitrogen), the tissue was washed three times with HBSS and digested with 0.04% trypsin (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... 5 × 105 landing pad cells expressing hACE2 with mNG21-10 were seeded in 6-well plates (Fisher Scientific). The cells were then then incubated at 37 °C with 5% CO2 for 15 mins to allow seeding ...
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bioRxiv - Neuroscience 2022Quote: ... Tax ID 10090) protein database (2017-6-7) using Proteome Discoverer (PD) 2.2 (Version 2.2.0.388; Thermo Fisher Scientific). Label-free quantification was also performed with PD 2.2 using precursor ions quantifier nodes ...
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bioRxiv - Cell Biology 2022Quote: ... All experiments were performed between passages 4 to 6 where cells were dissociated using TrypLE express (Life technologies), a gentle animal origin-free recombinant enzyme and seeded at a cell density of 5000 cells/cm2.
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bioRxiv - Cell Biology 2022Quote: ... Bone chips were plated in 12-well and 6-well plates in MEM-A medium (GIBCO, 12561-056) supplemented with 10% FBS (Cytiva ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were separated on 6% 0.5X TBE polyacrylamide gels and analyzed using LightShift® Chemiluminescent EMSA Kit (ThermoFisher).
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bioRxiv - Cell Biology 2022Quote: HCT116 cells were cultured in a 6-well plate at 300,000 cells per well in 3 ml McCoy’s 5A medium (Gibco) supplemented with 10% heat inactivated FBS and with 90 UI/mL Penicillin ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by growth in the absence of IL-2 for 6 - 9 h in RPMI 1640 (Gibco, # 21875034) medium supplemented with 10% FBS (Gibco ...
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bioRxiv - Molecular Biology 2021Quote: ... HL-1 cells in 6-well plates were transfected with 15 µL Lipofectamine 2000 reagent (Life Technologies 11668019) complexes with nucleic acid ...
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bioRxiv - Neuroscience 2021Quote: ... App gene expression level was assessed using Taqman probe (FAM-Mm01344172_m1) on a QuantStudio 6 Flex (Applied Biosystems) and normalized to Gapdh.
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bioRxiv - Neuroscience 2020Quote: ... according to the protocol below on a LightCycler 480 Instrument or the QuantStudio 6 Flex instrument (Applied Biosystems) in a 96-well format ...
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bioRxiv - Microbiology 2020Quote: ... 1976) and maintained in 6% O2 and 5% CO2 and grown in sterile filtered RPMI 1640 media (Gibco) supplemented with 2 M HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... Coverslips were mounted on slides with proLong gold antifade reagent with 4’,6-diamidino-2-phenylindole (Invitrogen, USA), which was used to probe for the nucleus.
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bioRxiv - Neuroscience 2020Quote: ... treated coverslips and incubated at 37°C for 2-6 hours in Dulbecco’s modified Eagle’s medium (F-12/DMEM; Invitrogen) supplemented with 1% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2021Quote: ... Supernatant was carefully aspirated and cells were resuspended in fresh media and plated in 6-cm plates (Gibco). Additional cell debris was aspirated off and fresh media added daily until cells reached confluency ...
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bioRxiv - Bioengineering 2021Quote: ... and subjected to heat- induced antigen retrieval using sodium citrate buffer(10 mM, pH 6, ThermoFisher, Waltham, MA) at >80°C ...
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bioRxiv - Neuroscience 2020Quote: Neocortex from C57Bl/6 pups (postnatal day 1-3) was dissected in Hibernate-A medium (#A1247501, Gibco, USA), digested with papain (#LS003124 ...
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bioRxiv - Microbiology 2021Quote: HEK293T cells seeded in 6 well plates were transfected with the indicated plasmid combinations using Lipofectamine reagent (INVITROGEN). At 24 hours post transfection ...
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bioRxiv - Microbiology 2022Quote: ... the cells were rinsed in PBS and the nucleus stained with 4′,6-diamidino-2-phenylindole (DAPI, ThermoFisher). Coverslips were then mounted and imaged with the Zeiss Laser Scanning 710 confocal microscope images were processed using Zeiss Zen 2011 software.
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bioRxiv - Molecular Biology 2019Quote: ... Total RNA was extracted from 6 pairs of OSCC and normal buccal pouch samples using TRIzol (Invitrogen, USA) according to the protocol provided by the manufacturer ...
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bioRxiv - Neuroscience 2019Quote: ... they were transfected with plasmid expression constructs in 6-well tissue culture plates (Thermo Fisher Scientific, Burlington, ON) using Lipofectamine 3000 transfection reagent (Life Technologies ...
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bioRxiv - Cancer Biology 2019Quote: ... 240 μl of the lysate was digested with 6 μl of RNase I (AM2294, 100 U/μl Invitrogen) at room temperature with rotation ...
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bioRxiv - Neuroscience 2019Quote: Neurons were grown on 6 cm dishes and treated with or without 10 μM chloroquine (Fisher Scientific, ICN19391980) in maintenance medium for 16 h ...
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bioRxiv - Cell Biology 2020Quote: ... at 37°C and 5% CO2 with 6 mL HDF medium consisting of 1x Advanced DMEM (Gibco™), 1x Glutamax™ (Gibco™) ...
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bioRxiv - Genomics 2020Quote: ... the cells were split 1:6 with Accutase (StemPro® Accutase® Cell Dissociation Reagent, Gibco™ A1110501), onto matrigel substrate in the presence of 10 μM of rock inhibitor (Y-27632 dihydrochloride ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Samples of the visceral yolk sac (n=6/group) were immersed in RNA Later solution (Invitrogen, MA, USA) and frozen at −20°C until further processing ...
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bioRxiv - Bioengineering 2020Quote: ... Stained sections were mounted in Slow Fade GOLD with 4’,6-diamidino-2-phenylindole (DAPI) (Molecular Probes, OR) and observed using a Nikon T300 Inverted Fluorescent microscope (Nikon Corp. ...
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bioRxiv - Systems Biology 2021Quote: ... splenocytes were obtained from naïve C57BL/6 mice and stained with CellTrace Pacific Blue Cell Proliferation Kit (ThermoFisher). Stained splenocytes (2.5 × 105 ...
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bioRxiv - Molecular Biology 2020Quote: ... and size selected for 130-200 nt fragments on a pre-casted 6% polyacrylamide Novex TBE gel (Invitrogen). Gel extraction was done using Gel breaker tubes (IST Engineering Inc ...
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bioRxiv - Neuroscience 2021Quote: ... Brain slices were incubated in 4’,6-diamidino-2-phenylindole (DAPI, Acros Organics–Thermo Fisher Scientific, Geel, Belgium) and mounted on microscope slides in mounting medium (Vectashield ...
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bioRxiv - Cancer Biology 2020Quote: ... mitochondria were purified from 4-6 × 107 cells using mitochondria isolation kit for cultured cells (Thermo Fisher Scientific) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... qRT-PCR reactions were performed in triplicate using QuantStudio 6 Flex Real-Time fast PCR System (Applied Biosystems) and 2-ΔΔCt values were used to calculate the relative expression level of the target genes compared to controls ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... PDMS coverslips were manually added to each well of 96/6 well plates (ThermoFisher Scientific, Waltham, MA USA) and each plate was subsequently sterilized ...
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bioRxiv - Developmental Biology 2019Quote: ... 6 mM β-mercaptoethanol and 0.5% NP-40) supplemented with 75 units/ml RNAse inhibitor (Thermo Fisher Scientific) and 1X mammalian protease inhibitor cocktail (Roche ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and 6 μg of pcDNA-SARS-CoV-2-S(ΔC19) in 1 ml of Opti-MEM medium (ThermoFisher). Then ...
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bioRxiv - Microbiology 2019Quote: ... The slides were mounted using ProLong diamond antifade mountant with DAPI (4′,6′-diamidino-2-phenylindole) (Invitrogen P36962).
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bioRxiv - Synthetic Biology 2019Quote: ... 6 mM L-glutamine (2 mM from Gibco 31600-091 and 4 mM from additional Gibco 25030-081), penicillin (100 U/µL) ...
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bioRxiv - Systems Biology 2020Quote: ... diluted to 100mM HEPES and 4M GnHCl and pH adjusted pH6.5-7.5) prior to 6-plex TMT labelling as per manufacturer’s instructions (Sixplex TMT, Cat# 90061, ThermoFisher Scientific). Equal ratios of TMT-labelled samples were pooled and methanol/chloroform precipitated ...
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bioRxiv - Bioengineering 2021Quote: The 6 most concentrated fractions were concentrated and desalted with 6mL 3MWCO protein concentrator columns (Pierce, Thermo Fisher) at 4C 4000xg 4 hrs and stored at 4C ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were transfected with 6 μg plasmid DNA per 106 cells using Neon transfection system (Thermo Fisher Scientific) using parameters 1400 V ...
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bioRxiv - Immunology 2020Quote: ... Cells were then stained with 1 μg/mL 4′,6-diamidino-2-phenylindole (DAPI; Molecular Probes/Thermo Fisher) and analysed on a FACSVERSE (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... Cells were then stained with 1 μg/mL 4′,6-diamidino-2-phenylindole (DAPI; Molecular Probes/Thermo Fisher) and analysed on a FACSVERSE (BD Biosciences) ...
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bioRxiv - Developmental Biology 2019Quote: ... day 4- and day 6-control and mutant ESC-derived neurospheres were collected and treated with Trizol (Invitrogen). RNAs were quantified by a Nanodrop instrument ...
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bioRxiv - Molecular Biology 2020Quote: mESCs were dissociated with Accutase™ and seeded in Nunclon Sphera 6-well plates (Thermo Fisher Scientific, # 174932) at a cell density of 11,000 cells / well in Serum + LIF medium ...
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bioRxiv - Cell Biology 2019Quote: ... RFL-6 cell were purchased from the American Type Culture Collection (ATCC) Ham’s F-12K medium (Thermo Fisher) supplemented with 20% FBS ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were passaged 1:2-1:6 every 2-5 days by being rinsed once with DPBS (Gibco) and dissociated using 0.5 mM EDTA (75 μl/cm2 ...
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bioRxiv - Biochemistry 2019Quote: ... or mismatch plasmids (1.5 μg per well in 6-well plate) using Lipofectamine 3000 Reagent (Thermo Fisher Scientific) following the manufacturer’s protocol.