Labshake search
Citations for Thermo Fisher :
201 - 250 of 8899 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2020Quote: ... Each sgRNA expression cassette was then shuffled into the respective binary vector by LR clonase (Invitrogen). All primers used here are listed in Table S3.
-
bioRxiv - Molecular Biology 2020Quote: ... the sgRNA cassette was PCR amplified from genomic DNA using Amplitaq Gold DNA Polymerase (Invitrogen, 4311820) and primers (F2 ...
-
bioRxiv - Immunology 2022Quote: ... placed in cassettes and fixed in 10% formalin for H&E staining or Carnoy solution (ThermoFisher) for fluorescent in situ hybridization (FISH ...
-
bioRxiv - Genetics 2022Quote: ... 1 mM DTT) using a Slide-A-Lyzer G2 Dialysis Cassette (10 kDa cutoff, ThermoFisher Scientific), and quantified with the BCA Protein Assay Kit (Pierce ...
-
bioRxiv - Immunology 2021Quote: ... Eluted proteins were dialyzed against PBS in Slide A_Lyzer Dialysis Cassettes (Thermo Fisher Scientific, Waltham, USA) and stored in aliquots in 50% glycerol at −20°C ...
-
bioRxiv - Biochemistry 2019Quote: ... 1.5 mM NaN3 using a slide-A-lyzer dialysis cassette (12-14 kDa MWCO, Thermo Scientific) at 4 °C for 15 h ...
-
bioRxiv - Cell Biology 2021Quote: ... The eluates were exchanged to SUMO protease buffer using Slide-A-Lyzer cassettes (Thermo Fisher Scientific) at 4°C overnight ...
-
bioRxiv - Microbiology 2021Quote: ... The resulting cassette was subcloned into the modified pCAGGS vector using the Gateway cloning system (Invitrogen) following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... and subsequently into tissue cassettes and embedded in paraffin using an automatic tissue processor (Thermo Scientific Excelsior AS ...
-
bioRxiv - Cell Biology 2021Quote: ... 1 mM DTT) and dialysed overnight on Dial-A-Lyzer Mini 3,500 MWCO cassettes (Thermo Scientific) at 4 °C into LoSalt buffer (30 mM HEPES pH 8 ...
-
bioRxiv - Cell Biology 2021Quote: ... 1 mM DTT) and dialysed overnight on Dial-A-Lyzer Mini 3,500 MWCO cassettes (Thermo Scientific) at 4 °C into LoSalt buffer (30 mM HEPES pH 8 ...
-
bioRxiv - Biochemistry 2022Quote: ... Inverter cassettes based on the SrpR and PhlF repressors were obtained as synthetic genes (GeneArt, ThermoFisher) and were likewise introduced into pCrepusculo ...
-
bioRxiv - Cell Biology 2022Quote: ... 2 mL of SMA was injected into a 20 kD dialysis cassette (Thermo Fisher Scientific; 66003) and placed in 4 L of SMA buffer with gentle mixing for 5 hrs at room temperature ...
-
bioRxiv - Molecular Biology 2022Quote: ... Formulations were dialyzed against PBS (pH 7.2) in Slide-A-Lyzer dialysis cassettes (Thermo Fisher Scientific) for at least 24 h ...
-
bioRxiv - Synthetic Biology 2022Quote: ... The dCas9-SSSavi_BirA cassette was subsequently inserted into PB_CAH (piggyBac_pCAGG_Amp_gateway_Hygro) using LR Clonase II (Thermo Fisher, #11791020) to create the plasmid PB_dCas9-SSSavi_BirA.
-
bioRxiv - Neuroscience 2022Quote: ... and 150 mM NaCl using a 7000 MWCO Slide-A-Lyzer Dialysis Cassette (Thermo Fisher Scientific). His-tagged proteins were eluted with the buffer containing 50 mM Tris–HCl ...
-
bioRxiv - Biophysics 2022Quote: ... the respective solutions were dialysed in Slide-A-Lyzer dialysis cassettes (2 kDa MWCO, Thermo Scientific) over night at 4 °C in 300 mM glycine ...
-
bioRxiv - Plant Biology 2023Quote: ... Purified protein was exchanged into PBS (pH 7.4) using Slide-A-Lyzer G2 dialysis cassettes (ThermoFisher) and stored at 4°C until use ...
-
bioRxiv - Biophysics 2023Quote: ... and 1 mM DTT) in a 10kDa cut off Slide-A-Lyzer Cassette (Thermo Scientific #66380) and applied to a Hi Trap SP HP column (GE Healthcare #17-1151-01 ...
-
bioRxiv - Cell Biology 2023Quote: ... apart from blots against NIPBL which were separated using 3-8% Tris-Acetate cassettes (Invitrogen, EA0375BOX) and 1 x Tris acetate running buffer (Invitrogen ...
-
bioRxiv - Plant Biology 2023Quote: ... the whole sgRNA cassette was transferred into pFH1 (pUB-CAS9) via Gibson cloning (Thermo Fisher Scientific). Following Rhizobacter transformation and floral dipping ...
-
bioRxiv - Molecular Biology 2023Quote: ... The homology repair template cloned in pUC19 consisted of a synthesised P2A-GFP cassette (Life Technologies) flanked by the GBP1 homology arms that match coordinates ...
-
bioRxiv - Microbiology 2023Quote: ... dialyzed against 1X MOPS using a 10 kD molecular weight cut-off cassette (Thermo Fisher Scientific), and sterilized using a liquid autoclave cycle (15-minute sterilization time ...
-
bioRxiv - Microbiology 2024Quote: ... Formulations were dialyzed against PBS (pH 7.2) in the dialysis cassettes (Thermo Scientific, Rockford, IL, USA) for at least 18 h ...
-
bioRxiv - Cell Biology 2024Quote: ... and 90% of samples were dialyzed using 3.5 MWCO Slide-A-Lyzer cassettes (Thermo Scientific; 66330) in RIPA buffer with gentle stirring overnight at 4°C ...
-
bioRxiv - Immunology 2023Quote: ... Formulations were dialyzed against PBS (pH 7.2) in Slide-A-Lyzer dialysis cassettes (Thermo Fisher Scientific) for at least 24 h ...
-
bioRxiv - Genomics 2020Quote: ... and gel imager (E-Gel Safe Imager connected to E-Gel iBase, Invitrogen), according to the recommendations provided by the manufacturer ...
-
bioRxiv - Genetics 2021Quote: ... E-Gel™ SizeSelect™ agarose gels (Invitrogen) were used to select DNA fragments for 250–300 bp ...
-
bioRxiv - Microbiology 2021Quote: ... gels using SYBR Safe DNA gel stain (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Synthetic Biology 2024Quote: ... E-Gel™ EX Agarose Gels (Thermo Scientific) were also utilized for sample analysis ...
-
bioRxiv - Cell Biology 2020Quote: ... Beads were pelleted and the supernatant was loaded into dialysis cassettes (3.5K, Slide-A-Lyzer, Thermo Scientific) that were then suspended in lysis buffer and dialyzed overnight to remove glutathione.
-
bioRxiv - Molecular Biology 2021Quote: ... The protein was dialyzed in a Slide-a-Lyzer cassette with a 3 kDa cutoff (Thermo Scientific) in step-wise gradient of NaCl and imidazole before final dialysis for 16-18h in 50 mM Tris-HCl ...
-
bioRxiv - Molecular Biology 2021Quote: ... A SV40 polyA-PGK promoter-BlasticidinR cDNA-bGH polyA cassette was then synthesized by GeneArt (Thermo Fisher), PCR amplified ...
-
bioRxiv - Genomics 2020Quote: Expression cassettes consisting of the doxycycline-inducible cytomegalovirus (CMV) promoter (derived from the Invitrogen T-REx system), the intron-containing EF1α 5 UTR ...
-
bioRxiv - Synthetic Biology 2019Quote: ... desalted by dialysis (Slyde-A-Lyzer™ dialysis cassettes, MWCO 20 kDa, Thermo Fisher Scientific, Waltham, USA) against desalting buffer (8 mM Na-phosphate buffer pH 7 ...
-
bioRxiv - Biochemistry 2020Quote: ... directly to the eluate and the solution dialyzed (Slide-A-Lyzer dialysis cassettes, Thermo Scientific, 3500 MWCO) overnight at room temperature (rt ...
-
bioRxiv - Cell Biology 2021Quote: ... Protein-containing fractions were dialyzed using a Slide-A-Lyzer Dialysis Cassette 3,500 MWCO (Thermo Scientific, 66110) to remove the imidazole ...
-
bioRxiv - Plant Biology 2021Quote: ... pH 5.2 (ZelluTrans, Carl Roth, or Slide-A-Lyzer Dialysis Cassette, Thermo Fisher Scientific, each 3.5 kDa) before loading onto a cation exchange HiTrapSP FF column (GE Healthcare ...
-
DNA uptake by cell wall-deficient bacteria reveals a putative ancient macromolecule uptake mechanismbioRxiv - Microbiology 2022Quote: ... the solution was directly loaded in a 10k MWCO dialysis cassette (Slide-A-Lyzer™, Thermo Scientific) and dialyzed against 1x Phosphate Buffered Saline (PBS ...
-
bioRxiv - Microbiology 2022Quote: ... and was dialyzed three times against PBS in a 3.5kDa Slide-A-Lyzer dialysis cassette (Thermo Scientific). Antibody specificity was determined by Western Blot using the parental wild-type and corresponding deletion mutants.
-
bioRxiv - Microbiology 2021Quote: ... The enzyme solution was loaded into a hydrated 10,000 MWCO Slide-a-lyzer dialysis cassette (Thermo Scientific), and the enzyme solutions were dialyzed in a 50 mM sodium phosphate buffer at 4°C ...
-
bioRxiv - Microbiology 2021Quote: ... The aquatic fraction was dialyzed for 48 h with Slide-A-Lyzer dialysis cassettes by Thermo Fisher, with a 3,500 molecular weight cut-off ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The supernatant was removed and loaded into a 10 kDa molecular weight cutoff dialysis cassette (Thermo Fisher). Lysate was dialyzed in 1 liter of S30B buffer (14 mM magnesium glutamate ...
-
bioRxiv - Biochemistry 2021Quote: ... CBC-HapX-DNA preparations were dialyzed (Slide-A-Lyzer Dialysis Cassette G2, MWCO 10 kDa; Thermo Fisher) against SEC Buffer (20 mM Tris/HCl ...
-
Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: ... pFastBac1-DEST was generated by amplifying the destination cassette (ccdB and chloramphenicol resistance genes) from pDESTTM15 (ThermoFisher) with flanking attR1/R2 sites (Table S7 ...
-
bioRxiv - Biochemistry 2022Quote: ... The samples were buffer exchanged to HNE using Slide-A-Lyzer dialysis cassettes (MWCO 10 kDa; ThermoFisher) at +4 °C for 1h with gentle agitation and the HNE was replaced with fresh HNE ...
-
bioRxiv - Genetics 2022Quote: ... the universal intermediate vector pFlexibleDT-ReCOIN was constructed through recombination of ReCOIN cassette into NotI (Thermo Fisher) and HindIII (Thermo Fisher)-digested pFlexibleDT using ClonExpress MultiS One Step Cloning Kit (Vazyme Biotech) ...
-
bioRxiv - Plant Biology 2022Quote: ... The resultant gRNA expression cassettes were transferred into pMpGE017 by using LR clonase II (Thermo Fisher Scientific) to generate pMpGE017_MpTIR1_5′gRNA1/2_3′gRNA3/4 ...
-
bioRxiv - Biophysics 2023Quote: ... and 0.02% NP40 substitute) in a 10 kDa cut off Slide-A-Lyzer Cassette (Thermo Scientific #66380), and concentrated in an Amicon Ultra-4 Ultracell 30kDa centrifugal filter (Merck-Millipore #UFC803024) ...
-
bioRxiv - Immunology 2023Quote: ... and dialyzed by PBS using the Slide-A-Lyzer G2 Dialysis Cassettes (10K MWCO) (Thermo Fisher Scientific). Concentration of sCTLA-4 was estimated by ELISA (R&D Systems ...