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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for High mobility group protein B1 HMGB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: HEK293 FT (ATCC CRL-3216) and VERO (ATCC CCL-81) cell lines were cultured in DMEM high glucose media (Life Technologies) containing 10% heat-inactivated fetal bovine serum (Life Technologies) ...
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bioRxiv - Genetics 2023Quote: ... and human embryonic kidney 293 cells (HEK293) were purchased from ATCC and cultured in Dulbecco’s Modified Eagle Medium (DMEM) high glucose (Fisher Scientific, #MT10013CV) with 10% FBS at 37 °C and 5% CO2 ...
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bioRxiv - Physiology 2020Quote: ... and MOTS-c in each group was normalized to the total tissue protein concentration as determined by the Bradford protein assay (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2020Quote: ... HEK293 stably expressing the tetracycline (tet)-repressor (Flp-In T-REx HEK293, Invitrogen, Carlsbad, CA, USA) were cultured as above with the addition of 50 μg/mL zeocin and 5 μg/mL blasticidin (Invitrogen ...
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bioRxiv - Developmental Biology 2019Quote: ... BMP2 recombinant human protein (Thermo Fisher Scientific, #PHC7145) was applied for 1h on cells ...
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bioRxiv - Neuroscience 2020Quote: ... Recombinant full-length human G2019S-LRRK2 protein (Invitrogen) was used as a protein standard ...
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bioRxiv - Bioengineering 2022Quote: ... Human BMP-4 Recombinant Protein (Gibco, Catalog # PHC9534). For cell culture of iPSCs on niches ...
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bioRxiv - Cancer Biology 2022Quote: ... IL-8 Monocyte Recombinant Human Protein (ThermoFisher, PHC0884), and Recombinant Human CXCL1/GROα Protein (R&D Systems ...
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bioRxiv - Cell Biology 2022Quote: ... Vitronectin (recombinant human protein) was from Fisher Scientific.
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bioRxiv - Cell Biology 2024Quote: ... 1.61 nM EGF Recombinant Human Protein (Invitrogen, PHG0313), 1.1 μM hydrocortisone (Sigma-Aldrich ...
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bioRxiv - Biophysics 2019Quote: ... The protein was expressed in HEK293S GnTI− cells (ATCC CRL-3022) grown in Expi293 Expression Media (Life Technologies) to densities 2.5 × 106 cells ml−1 ...
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bioRxiv - Molecular Biology 2022Quote: HEK293-6E cells (used for protein expression) were cultured in expression medium (FreeStyle F17 (Gibco, Cat#A13835-01)) supplemented with 1% FBS (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... FreeStyle HEK293 cells (Thermo fisher scientific) were transfected with pcDNA3.1 vector encoding for HSulf-2 cDNA flanked by TEV cleavable SNAP (20.5 kDa ...
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bioRxiv - Cell Biology 2021Quote: Low-passage HEK293 cells (Thermo Fisher) were co-transfected with 10 μg lentiviral vector (pCDH-SYN1-sypHy ...
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A nepenthesin insert allosterically controls catalysis in the malaria parasite protease plasmepsin VbioRxiv - Microbiology 2022Quote: ... we transfected HEK293-F cells (ThermoFisher) using a modified version of the protocol in (41) ...
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bioRxiv - Biochemistry 2021Quote: HEK293 Flp-In T-Rex (Invitrogen), HeLa (Austin et al. ...
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bioRxiv - Biophysics 2021Quote: Suspension-adapted HEK293 Freestyle cells (Invitrogen) in serum free media (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: HEK293 TREx cells (Invitrogen Canada Inc.) were cultured in high-glucose Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Microbiology 2023Quote: ... the Expi-HEK293 cells (Thermo Scientific) were transfected with the mammalian expression plasmid pHLSec containing the recodonized SUB1 ...
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bioRxiv - Microbiology 2023Quote: ... and HEK293-S cells (ThermoFisher Scientific) were cultured in FreeStyle293 expression medium (Life Technologies) ...
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bioRxiv - Immunology 2023Quote: ... FreeStyle™ HEK293-F cells (Gibco) were co-transfected with two plasmids ...
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bioRxiv - Biochemistry 2020Quote: ... 6x-His-tagged constructs of human and murine SNED1 cloned into pCDNA5/FRT (Thermo Fisher, Waltham, MA) between the FseI and AscI sites were used to transiently transfect 293T cells to validate the anti-SNED1 antibody generated in this study (Supplementary Figure S1) ...
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bioRxiv - Neuroscience 2022Quote: The proteomics was performed by the serum after removing common high-abundance protein through using a Thermo Fisher’s serum High Abundance protein removal reag ent (High Select™ Top14 Abundant Protein Depletion Mini Spin Columns, A36370, Thermo fisher, US). The mass spectrometer Thermo Scientific Q Exactive was perfor med for Label-free quantification detection and data was analyzed by Proteome Disco verer 2.2 ...
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bioRxiv - Neuroscience 2020Quote: ... 5 μg of control rabbit IgG or HNRNPA2/B1 (Invitrogen, PA5-34939 antibodies diluted with total of 200 μl lysis buffer ...
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bioRxiv - Systems Biology 2020Quote: ... The sequentially used primary antibodies include HRP conjugated rabbit anti-HMGB1 (Thermo Fisher Scientific; PA5-22722), HRP conjugated rabbit anti-HDAC2 (Abcam ...
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bioRxiv - Immunology 2023Quote: ... Expression constructs for M35-V5/His and M27-V5/His (both in pcDNA3.1-V5/His, Invitrogen) have been described previously (Munks et al ...
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bioRxiv - Genetics 2022Quote: ... A high molecular weight protein ladder (Invitrogen, catalog# LC5699) was used in each gel to track 460 kDa proteins ...
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bioRxiv - Biophysics 2019Quote: Human embryonic kidney cells 293 (HEK293-6E, NRC, Canada) were cultured in FreeStyle F17 expression medium (Thermo Fisher Scientific, Waltham, MA) supplemented with 2 mM L-glutamine (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... N-terminal FLAG-tagged human MCC (P23508-1) constructs (pCMV2B vector) were transfected into HEK293 cells and selected with G418 (Gibco #10131035). G418-resistant cells were grown ...
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bioRxiv - Biophysics 2021Quote: Human wild type ABCG2 or ABCG2 R184A containing an N-terminal Flag-tag was expressed in HEK293-EBNA (Thermo Fisher Scientific) cells as previously described19 ...
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bioRxiv - Molecular Biology 2021Quote: ... the coding sequence encoding human TSPO (hTSPO) was amplified from cDNA previously generated by reverse transcription from total HEK293 mRNA isolated using Trizol (Life Technologies) and cloned into either a pENTR/SD/TOPO vector (Invitrogen ...
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bioRxiv - Genetics 2024Quote: Flag-tagged wild type and mutated human RBBP5 plasmid were transfected to HEK293 cells by lipofectamine 3000 according to manufacturer’s protocol (Invitrogen, CA, USA). Cells were lysed by RIPA buffer and 20μg of whole cell lysate were used to assess protein expression in Western blot ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK293 and MS2-tagged HEK293:24xMS2-NEAT1 cells were cultured in Dulbecco’s modified eagle’s medium (DMEM, Gibco) with 10% fetal bovine serum (FBS ...
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bioRxiv - Genetics 2023Quote: ... 30 μg of protein per group were separated by gel electrophoresis (NuPage 4-12% Bis-Tris, Invitrogen) and transferred onto a nitrocellulose membrane (Amersham Protran 0.2 μm) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... HEK293 cells were a gift from Dr Harvey McMahon and were cultured in high-glucose DMEM supplemented with GlutaMAX (Thermo Fisher, 31966047) and 10% FBS (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: Plasmids encoding for recombinant His-tagged proteins were transformed into Escherichia coli BL21(DE3) (Invitrogen). Bacterial cultures were grown at 37°C in LB media to OD600 of 0.6-0.8 and induced with 0.5 mM IPTG at 18 °C for 16h (overnight) ...
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bioRxiv - Cell Biology 2019Quote: ... The His-mCherry-fusion proteins were purified using a Ni-NTA column (Thermo Fisher Scientific). The eluted proteins were dialyzed in 1 L dialyzed buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... His-tagged proteins were visualised using a primary anti-6xhis antibody (ArtNr: 11533923, Fisher Scientific), a HRP-coupled secondary antibody (ArtNr ...
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bioRxiv - Neuroscience 2023Quote: ... The His-tagged dAsapPZA protein was expressed and purified following the manufacturer’s guidelines (Invitrogen, USA).
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bioRxiv - Immunology 2021Quote: ... Group 5 (North American, Affymetrix), and Group 6 (non-Dutch/non-French European ...
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bioRxiv - Genomics 2021Quote: Electrophoretic Mobility Shift Assay (EMSA) was carried out using LightShift™ Chemiluminescent EMSA Kit (20148, ThermoFisher Scientific). Untreated and cytokine treated MIN6 nuclear extracts (NEs ...
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bioRxiv - Molecular Biology 2023Quote: ... SW13 human adrenocortical carcinoma cells were cultured in high glucose DMEM (Invitrogen) supplemented with 10% fetal calf serum and antibiotics ...
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bioRxiv - Bioengineering 2021Quote: The HHH peptide (Cys-biotin-PEG2-Gly-His-His-His-Gly(COOH)) was purchased from Thermo Fisher. It was 84% pure as determined by mass spectrometry ...
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bioRxiv - Microbiology 2020Quote: The full length CPSF6 protein was expressed in a suspension-adapted HEK293 cell line (Expi 293F, Thermo Fisher Scientific) by transfection of expression plasmid using ExpiFectamine 293 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... cell or nuclear lysates (400 ug) prepared from HEK293 or HDFs to Dynabeads Protein A (10001D, Thermo Fisher Scientific) that were prebound with 2–3μg of indicated antibody and incubated overnight at 4°C ...
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bioRxiv - Bioengineering 2023Quote: Blood plasma extracted from each group (n=5) was analyzed using the Inflammation 20-Plex Human ProcartaPlex™ Panel (Invitrogen). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: The cDNA sequence of human Sph1 was subcloned from the pcDNA3.1/V5-His-TOPO expression vector (Invitrogen, USA) 62 into the Venus-BiFC plasmids previously described 37 ...
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bioRxiv - Neuroscience 2022Quote: Human embryonic kidney (HEK293T) and human neuroblastoma (SH-SY5Y) cells from ATCC were cultured in high glucose DMEM (Invitrogen) and DMEM-F12 (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was extracted and quantified from WwoxWT/WT and WwoxP47T/P47T n=6 mice/group (3 males and 3 females) and cDNA was synthesized using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) following manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the labeled peptides of each group were mixed in equal amounts and fractionated by a high pH reversed-phase peptide fractionation kit (Thermo Scientific). The column was first balanced by acetonitrile and 0.1% trifluoroacetic acid (TFA) ...