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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for High mobility group protein B1 HMGB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... Each protein was first purified by metal affinity chromatography using 2ml of His purTM cobalt column (Thermo Scientific). The tag was removed by cleavage with TEV protease followed by purification on a second cobalt affinity chromatography ...
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bioRxiv - Cell Biology 2022Quote: His-CLASP1 protein was expressed in baculovirus-infected Sf9 insect cells using the Bac-to-Bac system (Invitrogen). After the first amplification ...
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bioRxiv - Cell Biology 2023Quote: ... entry clones containing individual TSWV proteins were recombined with a pIB/V5-His destination vector (Thermo Fisher Scientific) containing either GFP or mRFP in both amino and carboxy terminal fusions when possible ...
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bioRxiv - Cell Biology 2024Quote: LZTR1WT and LZTR1L580P were expressed as C-terminal His-tagged proteins in Expi-293F cells (Thermo Fisher Scientific). pcDNA3.1-LZTR1-Myc-6xHis plasmid was a gift from Jens Kroll (Heidelberg University and German Cancer Research Center (DKFZ-ZMBH Alliance)).56 The LZTR1L580Pvariant was introduced into the plasmid by site-directed mutagenesis as previously described.11 Cells were transfected using ExpiFectamine 293 Reagent (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: The DNA sequences of the human CE GTase and each sequence variant were synthesised and subcloned into the PGEX6p1-C-His plasmid vector by Thermo Fisher GeneArt ...
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bioRxiv - Neuroscience 2021Quote: His-tagged human dynamin 1 was expressed using the Bac-to-Bac baculovirus expression system (Thermo Fisher Scientific, Waltham, MA, USA) and purified as described previously (66) ...
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bioRxiv - Immunology 2021Quote: ... were cloned into pCEP4 mammalian expression vector containing N-terminal human Ig kappa leader sequence and C-terminal Avi-tag and His-tag (Invitrogen, USA). Expi293-Freestyle cells cultured at 37°C and 8% CO2 in growth medium containing Expi293 Expression Medium at 3×106/mL in 50 mL media were transfected overnight at 37 °C with 50μg of plasmid in 160μL of ExpiFectamine plus 6mL of OptiMEM-I (all from Invitrogen) ...
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bioRxiv - Physiology 2021Quote: ... The human full-length BACE1 or BACE2 cDNAs were expressed from the pcDNA3.1/myc-His expression vector (Invitrogen, Waltham, MA, USA).
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bioRxiv - Molecular Biology 2021Quote: ... with a Spectra™ Multicolor High Range Protein Ladder (ThermoFisher; Cat #: 26625). Samples were transferred to an Immobilon-P PVDF Membrane (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... Biotinylated proteins were purified on High Capacity Streptavidin Agarose beads (Thermo Scientific) in RIPA buffer and proteins were identified by mass spectrometry.
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bioRxiv - Immunology 2020Quote: ... and incubated with Protein G high-capacity agarose beads (Thermo Fisher Scientific) for IgG purification ...
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bioRxiv - Immunology 2021Quote: Maxisorp high protein binding 96 well ELISA plate (Nunc, Thermo Fisher Scientific.) was coated with 2μg/mL goat anti-human Fc antibody (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: Maxisorp high protein binding 96 well ELISA plate (Nunc, Thermo Fisher Scientific.) was coated with 2μg/mL goat anti-human Fc antibody (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... HEK293S GnTl- cells were cultured in Freestyle 293 (GIBCO) supplemented with 2% FBS and 1% Antibiotic-Antimycotic ...
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bioRxiv - Evolutionary Biology 2021Quote: ... HEK293 cells were cultured in DMEM medium (Gibco, USA) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK293 cells were cultured in DMEM medium (Gibco/BRL) supplemented with 10% (v/v ...
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bioRxiv - Genetics 2019Quote: HEK293 cells were maintained in DMEM plus Glutamax (Gibco) supplemented with 10% heat-inactivated FBS in a humidified 37°C ...
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bioRxiv - Immunology 2021Quote: ... Lentiviral particles were produced in HEK293-FT cells (Invitrogen). Then ...
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bioRxiv - Genomics 2021Quote: ... We cultured HEK293 cells in DMEM medium (Gibco, #11965092) with 10% fetal bovine serum and 1% penicillin– streptomycin ...
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bioRxiv - Microbiology 2020Quote: ... transfected with 293fectin into FreeStyle HEK293-F cells (Invitrogen), and purified from culture supernatants by sequential HisTrap Ni2+-NTA and Superdex 200 columns (GE Healthcare) ...
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bioRxiv - Molecular Biology 2020Quote: HeLa cells (ATCC CCL-2) and HEK293-FT (ThermoFisher) were cultured at 37 °C in 5% CO2 in medium composed of high glucose Dulbecco’s modified eagles medium (DMEM ...
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bioRxiv - Synthetic Biology 2021Quote: HEK293 cells were cultured in DMEM (Gibco; Life Technologies) with 10% FBS (Gibco ...
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bioRxiv - Synthetic Biology 2021Quote: HEK293 cells were cultured in DMEM (Gibco; Life Technologies) with 10% FBS (Gibco ...
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bioRxiv - Molecular Biology 2019Quote: HEK293 Flp-In™ T-Rex™ cells (Invitrogen) or HEK293T cells (ATCC ...
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bioRxiv - Genomics 2021Quote: HEK293 cells (ATCC) were cultured in DMEM (ThermoFisher Scientific) with 10% FBS (Atlanta Biologicals) ...
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bioRxiv - Cell Biology 2021Quote: ... Polyclonal HEK293 Flp-In T-REx cell lines (Invitrogen) were generated as described previously (Wyler et al. ...
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bioRxiv - Molecular Biology 2020Quote: HEK293 cells were dissociated with TrypLE (Thermo Fisher Scientific), washed ...
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bioRxiv - Biophysics 2020Quote: HEK293 cells were transfected using Lipofectamine 3000 (Thermo Fisher). SERCA2a constructs were expressed using the mammalian expression vector pcDNA3.1 with G418 resistance ...
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bioRxiv - Cell Biology 2020Quote: ... Flp-In T-Rex HEK293 (R78007, Thermo Fisher Scientific) and HaCaT (obtained from Joan Massague’s lab at Memorial Sloan Kettering Cancer Centre ...
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bioRxiv - Biophysics 2021Quote: ... HEK293S GnTI- cells cultured in Freestyle 293 medium (GIBCO) supplemented with 2% FBS at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... for HEK293 FT cells and Lipofectamine 2000 (Thermo Fisher) for fibroblast cell lines ...
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bioRxiv - Molecular Biology 2020Quote: HeLa S3 and HEK293 T-REx (Thermo Fisher Scientific) cells were maintained in DMEM supplemented with 10% FBS in a humidified incubator at 5% CO2 and 37°C ...
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bioRxiv - Genomics 2021Quote: HEK293 LTV cells were cultured in DMEM medium (Gibco), supplemented with 10% FBS at 37°C ...
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ADAR2 deaminase activity promotes Th17 effector function and protects against intestine inflammationbioRxiv - Immunology 2022Quote: The HEK293 cells were maintained in DMEM medium (Gibco) supplemented with 10% FBS and 50 U penicillin-streptomycin ...
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bioRxiv - Cancer Biology 2022Quote: ... HEK293 cells were transiently transfected using Lipofectamine 3000 (Invitrogen), following the manufacturer’s protocol.
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bioRxiv - Genomics 2022Quote: ... We cultured HEK293 cells in DMEM medium (Gibco, #11965092) with 10% fetal bovine serum and 1% penicillin–streptomycin ...
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bioRxiv - Biophysics 2022Quote: ... HEK293 and Jurkat cells were maintained in RPMI (Gibco) media containing 10% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 cells were cultured in Advanced DMEM (Life Technologies) supplemented with 10% FBS ...
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bioRxiv - Genomics 2023Quote: HEK293 cells were transfected with Lipofectamine 2000 (Invitrogen, 11668019) at a ratio of 2.8 ul lipofectamine per ug of DNA ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells were maintained in DMEM medium (Gibco, USA) supplemented with 1% penicillin-streptomycin solution (Gibco ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Flp-In T-REx HEK293 cells (Thermo Fisher Scientific) were co-transfected with this expression vector and pOG44 Flp-recombinase expression vector in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 and A549 cells were maintained in DMEM (Invitrogen) supplemented with 1% penicillin/streptomycin (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293 cells were resuspended in buffer R (Thermo Fisher), along with the RNP complex and ssODN template ...
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bioRxiv - Biochemistry 2023Quote: The HEK293 cell line was obtained from Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... HEK293S GnTl- cells were cultured in Freestyle 293 (GIBCO) supplemented with 2% (v/v ...
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bioRxiv - Bioengineering 2023Quote: ... and HEK293 or RPMI 1640 (ATCC modification) (ThermoFisher, A1049101) (for Jurkat cells complete with 5% pen/strep and 10% FBS ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293 cells were grown in RPMI 1640 medium (Gibco) supplemented with 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... HEK293 cells were transfected using lipofectamine 2000 (Thermofisher, USA) following the manufacturer protocol ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293 cells were propagated in MEM with GlutaMAXTM (Gibco) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293-FT (RRID: CVCL_6911) was purchased from Thermo Fisher Scientific (#R70007) ...