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Citations for Thermo Fisher :
51 - 100 of 10000+ citations for High mobility group protein B1 HMGB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: Human embryonic kidney cells (HEK293) were cultured in Dulbecco’s modified Eagle’s media (Gibco) containing 10 % v/v fetal bovine serum (heat-inactivated ...
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bioRxiv - Biochemistry 2020Quote: ... Human 28S and 18S rRNAs were extracted from HEK293 cells with TRIzol (Invitrogen). Intact rRNAs were isolated by pipetting from a native agarose gel after running the rRNA into wells in the center of the gel ...
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bioRxiv - Physiology 2022Quote: ... HEK293 cells were transfected with human TRPA1 expressing plasmid using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Human embryonic kidney (HEK293, ATCC #CRL-1573) cells were cultured in DMEM (Gibco), supplemented with 10% (vol/vol ...
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bioRxiv - Microbiology 2024Quote: ... the enriched peptides were analyzed using an Orbitrap Exploris 480 mass spectrometer equipped with a high-field asymmetric waveform ion mobility (FAIMS) Pro spectrometer and an EASY-nLC 1200 system (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2022Quote: MCF7 (female, CVCL_0031) and HEK293 cells were cultured in high-glucose DMEM medium (Gibco) supplemented with 10% fetal bovine serum (HyClone) ...
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bioRxiv - Molecular Biology 2023Quote: ... The Flp-In HEK293 cells were cultured in high glucose pyruvate medium (Gibco, #10569010), supplemented with 10% FBS (Gibco ...
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bioRxiv - Biophysics 2019Quote: ... The size of the TIM22 complex was determined based on the mobility pattern of a native protein molecular weight standard (cat number: LC0725, Invitrogen).
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bioRxiv - Cell Biology 2023Quote: ... the DNA-protein interaction was detected with a LightShiftTM Chemiluminescent electrophoretic mobility shift assay (EMSA) kit (Thermo Fisher Scientific, USA). The primers used for EMSA are listed in Table S2.
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bioRxiv - Cancer Biology 2021Quote: ... Cyclin B1 (Fisher Scientific, BDB554177), Phospho-Ser28-Histone3 (BioLegend ...
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bioRxiv - Microbiology 2020Quote: ... siRNA targeting SR-B1 (Ambion) and negative control scramble RNA (Ambion ...
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bioRxiv - Microbiology 2021Quote: HEK293/TLR4 and HEK293/null cells (both Invitrogen) were maintained in 4.5 g/L glucose DMEM (Gibco ...
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bioRxiv - Systems Biology 2020Quote: ... HRP conjugated rabbit anti-HMGB1 (Thermo Fisher Scientific; PA5-22722), HRP conjugated rabbit anti-TDP43 (Abcam ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 human embryonic kidney cells were cultured in Dulbecco’s modified Eagle medium (DMEM, Invitrogen), containing 10% FBS ...
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bioRxiv - Neuroscience 2020Quote: Human embryonic kidney293 (HEK293) cells were maintained in Dulbecco’s Modified Eagle’s Medium (Life Technologies) supplemented with 10% fetal bovine serum (Atlanta biologicals ...
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bioRxiv - Cell Biology 2021Quote: Human HEK293 suspension culture cells (Expi293F) were cultured in Expi293F expression media (Fisher Scientific) and maintained at 37°C in 8% CO2 in 125 mL spinner flasks on an orbital shaker rotating at 125 rpm ...
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bioRxiv - Biochemistry 2022Quote: The human embryonic kidney HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (Gibco) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution at 37 °C under 5% CO2 ...
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bioRxiv - Immunology 2024Quote: ... the lysate supernatant containing His-tagged proteins was affinity-purified with His-Tag magnetic beads (Invitrogen) and used for pull-down assays ...
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bioRxiv - Molecular Biology 2019Quote: ... All proteins were expressed in Hi-Five cells (Invitrogen) from Trichoplusia ni using Baculovirus and the cultures were harvested 60 hours after infection ...
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bioRxiv - Neuroscience 2020Quote: HEK293 cells were plated in 6-well plates and maintained in high glucose DMEM (Invitrogen) growth media supplemented with 1% pen/strep (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... HeLa cells and HEK293 were grown in high glucose DMEM (ThermoFisher, 11995-065; Biochrom, F0435) containing 2 mM L-glutamine (ThermoFisher ...
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bioRxiv - Systems Biology 2023Quote: HEK293 cells were grown and maintained in high-glucose (4.5 g/l) DMEM (Thermo Fisher) for BRET assays ...
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bioRxiv - Immunology 2022Quote: ... 1 mln HEK293 Flp-In T-REx cells were grown on DMEM High glucose (Gibco) supplemented with 10% fetal bovine serum on 6-well plates ...
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bioRxiv - Microbiology 2022Quote: ... Protein abundance samples were fractionated with Field Assymetric Ion Mobility Spectrometry (FAIMS) fractionation with a FAIMS Pro device (Thermo Fisher Scientific). Each pooled sample was analyzed four times with four FAIMS compensation voltages (CV ...
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bioRxiv - Microbiology 2020Quote: ... His tagged proteins were isolated from the supernatant using Dynabeads™ His-Tag Isolation and Pulldown (Invitrogen) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The His-tagged proteins were blotted using anti-6x His tag HRP-conjugated monoclonal antibody (Invitrogen, USA) and detected using Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... and RBD-his tag protein-bound antibody was detected with goat-anti-human IgG1 (H+L) with horseradish peroxidase (HRP) conjugated (Invitrogen,1:1000) The plates were washed and developed using TMB substrate solution (Biolegend ...
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bioRxiv - Cell Biology 2022Quote: Human HEK293 cells were cultured at 37 °C and 5% CO2 in DMEM (ThermoFisher Scientific), supplemented with 10% fetal bovine serum (ThermoFisher Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... The vectors were then transfected into human embryonic kidney cells (HEK293) with Fectin-system (Invitrogen). Protein from culture supernatant was isolated as previously described [46 ...
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bioRxiv - Molecular Biology 2023Quote: Human embryonic kidney fibroblasts (HEK293, RRID:CVCL_0045) were grown in Dulbecco’s Modified Eagle Medium (DMEM, ThermoFisher) supplemented with 10% fetal calf serum (FBS ...
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bioRxiv - Genomics 2020Quote: Control and HMGB1-knockdown were harvested in Trizol LS (Life Technologies) and total RNA was isolated and DNase I-treated using the DirectZol RNA miniprep kit (Zymo Research) ...
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bioRxiv - Microbiology 2020Quote: ... All human MARCH cDNAs were cloned into pCDNA3.1/myc-His A (Invitrogen) and were then subcloned into pBJ5 ...
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bioRxiv - Microbiology 2023Quote: ... human MARCH4 and mouse MARCH2 (mMARCH2) into pcDNA3.1/myc-His A (Invitrogen) and pBJ5 vector have been previously described (10) ...
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bioRxiv - Cell Biology 2021Quote: ... or CDK1/cyclin B1 (Thermo Fisher) in Phosphorylation Buffer (50 mM MOPS pH 7.4 ...
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bioRxiv - Developmental Biology 2023Quote: COS-7 and HEK293 cells were grown in on plastic dishes in high-glucose DMEM (Gibco) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Immunology 2022Quote: ... we first placed ITPRIPL1 protein with 6x-His antibody (Invitrogen) first and slowly rotated at room temperature for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... protein expression from plasmid (derivates of pBAD-His/B (Invitrogen) in all cases except expression of VirF from pCL5 [71] ...
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bioRxiv - Cancer Biology 2020Quote: ... His-tagged GOT2 protein was immunoprecipitated using the His-tag isolation and pull-down Dynabeads system (Thermo Fisher) using the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... The His SNX32ΔC protein bound to the lipids was detected by anti-His antibody [1:10,000 (Thermo Scientific)]
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bioRxiv - Cell Biology 2020Quote: ... HEK293 T-Rex (referred to as HEK293, cat.no. R71007, Invitrogen) and NIH3T3 cells were grown in DMEM Glutamax® (Thermo Fisher Scientific ...
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Modified N-linked glycosylation status predicts trafficking defective human Piezo1 channel mutationsbioRxiv - Biophysics 2020Quote: ... HEK293S GnT1-/- or HEK293 cells (ThermoFisher Scientific, Cat. No. R78007) using Lipofectamine 3000 transfection reagent (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: HEK293 cells (Invitrogen) were grown in DMEM (1g/L glucose ...
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bioRxiv - Synthetic Biology 2022Quote: HEK293 cells (ThermoFisher; further authenticated by assessing cell morphology and growth rate ...
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bioRxiv - Biochemistry 2023Quote: ... Ten3-B0/B1 monomeric (referred to as Ten3 from now on) proteins were diluted in HBSS (ThermoFisher) and used at a concentration of 10μg/mL in final stripe assays ...
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bioRxiv - Zoology 2021Quote: EMSA was performed using the Electrophoretic Mobility Shift Assays kit (Invitrogen E33075) according to manufacturer’s protocols ...
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bioRxiv - Biochemistry 2020Quote: Human embryonic kidney cells 293 (HEK293) cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco) supplemented with 10% fetal bovine serum (FBS ...
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An alternatively spliced TREM2 isoform lacking the ligand binding domain is expressed in human brainbioRxiv - Molecular Biology 2022Quote: HMC3 human microglia or HEK293 cells were transfected with Lipofectamine 3000 with Plus reagent (Invitrogen L3000001) per manufacturer instructions with 0.8 μL of Lipofectamine 3000 ...
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bioRxiv - Neuroscience 2021Quote: ... The plasmids were transfected into HEK293 human embryonic kidney cells using Lipofectamine2000 (Invitrogen, Carlsbad, CA, USA). The recombinant proteins were purified with anti-FLAG agarose affinity gel (A2220 ...
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bioRxiv - Biochemistry 2021Quote: Human HEK293S GnTI- cells were cultured as suspension at 37°C in FreeStyle293 expression medium (Invitrogen) supplemented with 2% (vol/vol ...
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bioRxiv - Neuroscience 2022Quote: Human embryonic kidney 293 cells (HEK293, ECACC) were cultured in Dulbecco’s Modified Eagle’s Medium (Thermo Fisher) with 10% fetal bovine serum (Hyclone) ...