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Citations for Thermo Fisher :
201 - 250 of 5122 citations for Cytomegalovirus Purified Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Antibody/antigen mixture was incubated with Dynabeads M-280 sheep anti-rabbit or mouse IgG (Thermo Fisher Scientific) for 6h at 4°C in the following day ...
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bioRxiv - Neuroscience 2023Quote: ... For antigen retrieval cells were incubated for ten minutes with 1 ug/ml proteinase K (Thermo Fisher Scientific) at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... 200 μL of the virus dilution containing 1µg-3µg p24 antigen was transferred to a poly-D-lysine coated glass coverslip (#1 chambered coverglass, Nunc™ Lab-Tek™ ...
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bioRxiv - Immunology 2023Quote: ... and coronavirus antigens were expressed in Expi293F cells by transient transfection in FreeStyle F17 expression media (Thermo Fisher) supplemented to a final concentration of 0.1% Pluronic Acid F-68 and 20% 4 mM L-glutamine using Expifectamine transfection reagent (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: ... the antigens were captured for 15 mins at 4 °C with 50 µl Streptavidin Magnetic beads (Thermo Fisher) which were previously incubated with superblock (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... Target antigens were finally detected using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, Waltham, MA). Images were scanned and analyzed using ImageJ software ...
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bioRxiv - Microbiology 2021Quote: ... gel purified (ThermoFisher Scientific) and Gibson assembled into pSRK (44 ...
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bioRxiv - Neuroscience 2023Quote: ... purified (MEGAclear Kit, Ambion), ethanol precipitated ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 antigens were coupled to magnetic Luminex beads (Luminex Corp) by carbodiimide-NHS ester-coupling (Thermo Fisher). Coupled beads were incubated for 2 hours at 37°C with serum samples (1:10 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... The slides were placed in 250 ml freshly made antigen retrieval solution (0.1 M sodium citrate buffer pH 6.0, Fisher Scientific) and pressure cooked (A ...
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bioRxiv - Immunology 2022Quote: ... The antigen binding fragment (Fab) portion of LY-CoV1404 was generated by proteolytic digestion using immobilized papain (ThermoFisher Scientific), followed by removal of un-cleaved protein using standard chromatography techniques ...
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bioRxiv - Systems Biology 2019Quote: Recombinant protein antigens were first labeled with biotin using EZ-Link™ NHS-LC-Biotin reagent (Thermo Fisher, #21366) and then hybridized with the PETAL array ...
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bioRxiv - Neuroscience 2021Quote: ... Immunoreactivity to the antigen was visualized using Alexa Fluor 488-or Alexa Fluor 594-conjugated secondary antibody (Molecular Probes). Nuclear staining was performed with Hoechst 33258 (Polysciences) ...
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bioRxiv - Microbiology 2020Quote: ... the cells were washed in PBS and incubated for 1-h at RT with 1 mL anti-Early Endosomal Antigen 1 (EEA1) monoclonal antibody (F. 43.1; Invitrogen) diluted 1:200 in blocking buffer ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit or sheep antigens were raised in donkey and conjugated to Alexa 488 or Alexa 594 (Molecular Probes, ThermoFisher, RRIDs ...
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bioRxiv - Immunology 2020Quote: ... Anti-mouse monoclonal antibodies targeting cell surface antigens included anti-GL7 (FITC, PE, eFluor660; clone GL 7; Thermo Fisher), anti-B220 (PE-Cy7 ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 antigens were coupled to magnetic Luminex beads (Luminex Corp) by carbodiimide-NHS ester-coupling (Thermo Fisher). Coupled beads were incubated for 2 hours at 37°C with serum samples (1:10 dilution ...
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bioRxiv - Immunology 2022Quote: Vaccine 5: The above mentioned hCMP-mRBD antigen when expressed from Stable CHO (Thermo Fisher Scientific, Cat no. R75807) cell lines was named as hCMP-mRBD-CHO ...
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bioRxiv - Cancer Biology 2020Quote: ... The beads with antigens captured were loaded onto a SDS-PAGE gel (4-20% gradient polyacrylamide) (Thermo Fisher Scientific) in duplicates ...
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bioRxiv - Neuroscience 2019Quote: ... The antigen peptide with N-terminus Cysteine (NH2-CGGGGAAPGPPRHG-COOH) was coupled with keyhole limpet hemocyanin (Thermo fisher 77600). The antigen was injected into rabbit and antisera were collected after primary and several booster injections ...
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bioRxiv - Cancer Biology 2021Quote: ... The antigen-antibody complexes on the membrane were detected using labeled secondary antibodies and enhanced chemiluminescence reagents (Thermo Scientific). The antibodies and their dilutions were as follows ...
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bioRxiv - Immunology 2020Quote: ... total IgG against these antigens were detected using HRP-conjugated respective anti-mouse antibody (Sigma-Aldrich, eBioscience and Invitrogen) and the reaction was detected using TMB substrate (BD biosciences) ...
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bioRxiv - Bioengineering 2022Quote: ... 4 μL of capture Ab (~4 μg/ml) or SARS-CoV-2 recombinant antigen (~0.2 mg/ml) in 1x PBS (Gibco) was loaded in each channel ...
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bioRxiv - Developmental Biology 2022Quote: ... were used to detect antigens and DNA was stained with DAPI in the mounting medium (ThermoFisher Scientific, Cat# P36941).
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bioRxiv - Physiology 2022Quote: ... Non-specific antigens were blocked for 60 min using a blocking buffer (Sea Block Blocking Buffer; ThermoFisher Scientific, USA). Then ...
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bioRxiv - Microbiology 2022Quote: All antigens and FcγRs were equilibrated in 1X PBS using Zeba-Spin desalting and size exclusion chromatography columns (ThermoFisher) prior to bead coupling ...
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bioRxiv - Cancer Biology 2023Quote: ... The enzymatic antigen retrieval was performed by incubating the sections for 2 min with TrypLE™ Express Enzyme (ThermoFisher) at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... The antigen-antibody complex was detected by SuperSignal™ West Dura Extended Duration Chemiluminescent Substrate (Cat#34075, Thermo Fisher).
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bioRxiv - Immunology 2021Quote: ... for 2-18 hours at 4°C and stained with cocktails of fluorescently-labeled antibodies against intracellular antigens diluted in Permeabilization Buffer (Invitrogen) for 20 minutes at room temperature followed by 20 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... anti-mouse surface antigen 1 (SAG1) mab DG52 1:500 46 and then with goat anti-rat-Alexa546 1:300 (Invitrogen), goat anti-rat-Alexa375 1:300 (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: All antigens were biotinylated using an NHS-Sulfo-LC-LC kit according to the manufacturer’s instruction (Thermo Fisher, MA, USA) if required by the assay and excessive biotin was removed by size exclusion chromatography using Zeba-Spin desalting columns (7kDa cutoff ...
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bioRxiv - Immunology 2021Quote: ... cells were fixed and permeabilized using the Foxp3/Transcription Factor Staining Buffer Set overnight and stained with antibodies against intracellular antigens (1 µg/ml) in Permeabilization buffer (ThermoFisher). For biotinylated antibodies ...
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bioRxiv - Immunology 2021Quote: ... Induced yeast libraries were stained for antigen binding (RBD-Fc APC tetramers) and scFv expression (chicken anti-c-myc IgY; Invitrogen). Following two washes in PBSF (1x PBS ...
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bioRxiv - Bioengineering 2019Quote: Paraffin-embedded aorta sections mounted slides were subjected to heat-induced antigen epitope retrieval with citrate buffer (Thermo Scientific, MA). The slides were incubated overnight at 4°C with primary antibodies for anti -MMP-9 (Rabbit anti-Mouse ...
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bioRxiv - Microbiology 2021Quote: ... fixed parasites were incubated with 5µg/mL Blood Group Antigen H (O) Type 1 monoclonal antibody (Invitrogen 13-9810-82), washed three times with 1x PBS and incubated with 1:100 α-mouse secondary antibody conjugated to Alexa 488 (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... with 50 µL per injection site of vaccine formulations containing 5 µg of SARS-CoV-2 antigen (RBD-SD1, S-2P, S-PCNA1, or 6RBD-np) gently mixed 1:1 (v/v) with AddaVax (Invitrogen) adjuvant ...
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bioRxiv - Immunology 2020Quote: ... Phage display selections were carried out by alternating between capture of the antigen on neutravidin coated wells on Maxisorp plates (Nunc) and streptavidin magnetic beads (Invitrogen ...
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bioRxiv - Immunology 2022Quote: The uptake and processing of CSF-derived protein by antigen-presenting cells outside the CNS was evaluated by injecting 3 μl of DQ-OVA (2 mg/ml, Invitrogen) into the CSF of chronically infected C57BL/6 mice by i.c.m ...
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bioRxiv - Immunology 2020Quote: ... surface antigen-labeled cells were fixed and permeabilized with the eBioscience Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific) and incubated with the fluorochrome-labeled antibodies listed in Table S3 ...
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bioRxiv - Microbiology 2021Quote: ... Mice received 20 μg protein antigen (vaccinated) or PBS (mock-vaccinated) mixed 1:1 with Imject Alum adjuvant (Thermofisher Scientific) in a total volume of 100 μl ...
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bioRxiv - Immunology 2020Quote: To address neutrophils activation and phagocytic capacities we used pHrodo-labeled BioParticles and coated with Staphylococcus aureus (S. aureus) or Zymosan antigens (Invitrogen). pH-sensitive pHrodo dyes are useful for visualizing acidification process during phago-lysosome formation (29) ...
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bioRxiv - Immunology 2020Quote: ... Each mouse received 50 μg of antigen in 150 μL of PBS (NP40‑FICOLL) or PBS/Alum (77161, Thermo Fisher) adjuvant (2:1 ratio ...
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bioRxiv - Immunology 2020Quote: ... braziliensis (MHOM/BR/1975/M2903) soluble antigen (2 μg/well) were adsorbed in microtiter plate (Nunc-immuno Plate, Roskilde, Denmark). Then ...
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bioRxiv - Immunology 2021Quote: ... Thymocytes were stained for extracellular antigens before EdU detection with click-iT Plus EdU Alexa Fluor 488 Flow Cytometry Assay Kit (Invitrogen), following the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... slides were placed in metal-free water for 5 minutes on an orbital shaker and then incubated in pH 9 IHC Antigen Retrieval Solution (Invitrogen) at 96°C for 30 min ...
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bioRxiv - Immunology 2021Quote: ... slides were placed in metal-free water for 5 minutes on an orbital shaker after which they were incubated in pH 9 IHC Antigen Retrieval Solution (Invitrogen) at 96°C for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... Primary cells were immortalized by transfection of pRSVAg1 plasmid expressing Simian Vacuolating Virus 40 large T antigen (SV40T) with lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... mice were injected sub-cutaneously with 10 μg of fluorescent antigen in 100 μL mixed 1:1 with Imject Alum Adjuvant (ThermoFisher) in the upper and lower flank to target the brachial ...
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bioRxiv - Immunology 2022Quote: ... for 2-18 hours at 4°C and stained with cocktails of fluorescently labeled antibodies against intracellular antigens diluted in Permeabilization Buffer (Invitrogen) for 30 minutes at 4°C.
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bioRxiv - Developmental Biology 2022Quote: ... Overnight incubations (4°C) of the sections were carried out with mouse monoclonal anti- proliferating cell nuclear antigen (PCNA) (1:200, Invitrogen). Control sections were incubated with a mouse IgG Isotype Control (1:200 ...