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Citations for Thermo Fisher :
1 - 50 of 5122 citations for Cytomegalovirus Purified Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Antigens were purified on C-tag column (Thermo Fisher) and quality-controlled by SDS-PAGE and for their level of endotoxin ...
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bioRxiv - Neuroscience 2023Quote: Purified antigen was immobilized on a 96 well immunosorbent plate (Nunc). All the following steps were done by gentle shaking on an orbital shaker ...
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bioRxiv - Pathology 2022Quote: ... under the control of a cytomegalovirus promoter (ViraPower Adenovirus Expression System, Invitrogen). The AAV-Tau was verified by sequencing and the production of Tau by western blot ...
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bioRxiv - Microbiology 2022Quote: ... Antigen-antibody complexes were selectively purified using Pierce Protein A Agarose (ThermoFisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2022Quote: All antigens were His-tagged and purified using HisPur™ Ni-NTA resin (ThermoFisher). Cell supernatants were diluted with 1/3rd volume wash buffer [20 mM imidazole ...
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bioRxiv - Immunology 2022Quote: Antigen proteins with hexahistidine tags were purified with HisPurTM Ni-NTA resin (Thermo Fisher). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... and affinity purified using the peptide antigens immobilised on a Sulfolink affinity column (ThermoFisher). Results shown were predominantly obtained using affinity-purified “303” and “304” anti-TbSmee1 antibodies ...
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bioRxiv - Molecular Biology 2023Quote: ... The amplicons were assembled with a 5′ cytomegalovirus (CMV) promoter amplified from pcDNA3.1 (Invitrogen) by Gibson assembly (New England Biolabs) ...
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bioRxiv - Microbiology 2022Quote: Purified S antigens (1µg/ml) were coated onto 96-well NUNC Maxisorp plates (Thermo Scientific) at room temperature (RT ...
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bioRxiv - Immunology 2020Quote: ... Purified antigens were biotinylated using an EZ-Link Sulfo-NHS-LCBiotinylation kit (Thermo Fisher Scientific) using a 1:1.3 M ratio of biotin to Ag ...
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bioRxiv - Cell Biology 2021Quote: ... and anti-TRIP4 antibodies were raised in rabbits against affinity purified N-terminally hexahistidine-tagged full-length proteins and purified with the respective antigens coupled to SulfoLink resin (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2024Quote: ... the purified antigens were then biotinylated with the EZ-Link Sulfo-NHS-Biotin (Thermo Fisher Scientific) using a 50:1 biotin to protein molar ratio for calculations.
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bioRxiv - Biochemistry 2022Quote: ... and FL Spike trimers from hCoVs polypeptide antigens) were purified using HisPur™ Ni-NTA resin (ThermoFisher). Cell supernatants were diluted with 1/3rd volume wash buffer (20 mM imidazole ...
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bioRxiv - Cell Biology 2020Quote: ... The antiserum was affinity purified using the antigen coupled to Dyna M-280 Streptavidin beads (Life Technologies).
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bioRxiv - Synthetic Biology 2023Quote: All RBD antigens were His-tagged and purified using HisPur Ni-NTA resin (Thermo Fisher Scientific, 88222). Cell supernatants were diluted with 1/3 volume of wash buffer (20 mM imidazole ...
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bioRxiv - Genomics 2020Quote: Expression cassettes consisting of the doxycycline-inducible cytomegalovirus (CMV) promoter (derived from the Invitrogen T-REx system), the intron-containing EF1α 5 UTR ...
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bioRxiv - Neuroscience 2023Quote: ... Antigen retrieval was performed with Low pH IHC Antigen Retrieval Solution (Invitrogen) in a pressure cooker set to 100°C for 10 minutes ...
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bioRxiv - Microbiology 2022Quote: Purified F antigens were biotinylated using an EZ-link Sulfo-NHS -LC-Biotinylation kit (Thermo Fisher, cat#A39257) with a 1:1.3 molar ratio of biotin to F ...
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bioRxiv - Molecular Biology 2021Quote: ... The antiserum was affinity-purified using the peptide antigen coupled to UltraLink™ Iodoacetyl gel (Thermo Fisher Scientific).
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Neutralizing activity of broadly neutralizing anti-HIV-1 antibodies against primary African isolatesbioRxiv - Immunology 2020Quote: ... The cytomegalovirus (CMV) promoter was amplified by PCR from the pcDNA 3.1D/V5-His-TOPO plasmid (Life Technologies) with forward primer 5’-AGTAATCAATTACGGGGTCATTAGTTCAT-3’ and reverse primer 5’ CATAGGAGATGCCTAAGCCGGTGGAGCTCTGCTTATATAGACCTC-3’ ...
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bioRxiv - Cancer Biology 2023Quote: ... antigen retrieval was performed using IHC Antigen Retrieval Solution (ThermoFisher, 00-4955-58) in a steamer for 40Dminutes ...
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bioRxiv - Immunology 2020Quote: ... Antigen-specific IgG was purified from pooled serum with UF6b-conjugated Dynabeads™ M-270 Epoxy (Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... and LacI antibodies were affinity-purified from serum using the appropriate antigen coupled to AminoLink Coupling Resin (Thermo Scientific) according to manufacturer’s protocol.
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bioRxiv - Cell Biology 2020Quote: ... Tor1A and Tor1B coding regions were amplified from HeLa cDNA and cloned into the vector pcDNA5.1-GFP FRT/TO (cytomegalovirus [CMV] promoter; Invitrogen) for generation of stable ...
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bioRxiv - Immunology 2022Quote: ... native control peptides and control antigens using a custom-designed antigen microarray (ThermoFisher Scientific, Immunodiagnostics)9 ...
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bioRxiv - Physiology 2022Quote: ... Targeted antigen retrieval was performed by boiling sections in 1x IHC antigen retrieval solution (Invitrogen) for 15-20 mins ...
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bioRxiv - Immunology 2020Quote: Serum antibodies were purified from 25mL of remaining serum of final bleeds using antigen conjugated to NHS-activated agarose resin (ThermoFisher). Columns were washed three times with 1X PBS buffer ...
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bioRxiv - Immunology 2023Quote: ... or control antigen PE (Thermo Fisher) were mixed with adjuvants per manufacturer’s protocols and added to cells immediately after plating ...
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bioRxiv - Immunology 2023Quote: ... Monomeric antigens were biotinylated (ThermoFisher; A39257) at an optimized ratio which resulted in <1 biotin per molecule ...
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bioRxiv - Molecular Biology 2021Quote: ... Antibodies were purified by antigen affinity chromatography using Thermo Scientific AminoLink Plus Coupling Resin according to the manufacturer’s protocol (Thermo Fisher Scientific). The generated antiserum was shown to recognize exclusively the germ cells in testes and early embryos and was used in Western-blot analysis (dilution 1:1000).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and a lab-made cytomegalovirus promoter-driven enhanced green fluorescent protein (GFP) reporter construct using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA). On day three ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: The coding region of each opsin transcript was amplified from eye cDNA and subcloned in a modified pFRT-TO expression vector cassette derived from pcDNA5 and containing the human cytomegalovirus (CMV) immediate early promoter (Invitrogen, USA). The expression plasmid was modified to include a C-terminal tag by the monoclonal antibody FLAG epitope sequence (DYKDDDDK) ...
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bioRxiv - Physiology 2020Quote: ... and a non-targeting pre-miRNA under bicistronic control of the cytomegalovirus (CMV) promoter in the pcDNA6.2GW/EmGFP-miR plasmid (Invitrogen, Carlsbad, CA). The Fbxl22 RNAi plasmid encodes EmGFP and an artificial pre-miRNA targeting the full length gene of mouse Fbxl22 under bicistronic control of the CMV promoter ...
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bioRxiv - Immunology 2020Quote: ELISA of SARS-CoV-2 antigens was performed by coating antigens on MaxiSorp 96-well plates (ThermoFisher) at 2 µg/mL in 1X PBS [pH 7.4] overnight at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... antigen retrieval techniques were applied human tissue sections using IHC Antigen Retrieval Solution (00-4956-58, Invitrogen). These tissues were stained with Abs against human CD4 pre-labeled with Alexa Fluor 488 using an Ab labeling kit (Molecular Probes ...
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bioRxiv - Microbiology 2019Quote: ... in the presence of 10 μg of purified Gn or NP antigens in 96 well V-bottomed culture plates (Nunc, Roskilde, Denmark). For intracellular detection of IFN-γ ...
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bioRxiv - Immunology 2023Quote: ... These plasmids were co-transfected with MLV (murine leukemia virus)-CMV (cytomegalovirus) luciferase and MLV Gag/Pol plasmids into HEK-293T cells (ATCC CRL-3216) using Lipofectamine 2000 (Thermo Fisher 11668019) transfection reagent following the manufacturer’s recommended protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... brain slices underwent an additional antigen retrieval step in 1X IHC Antigen Retrieval Solution (Invitrogen, 00-4956-58) for 15 minutes at 95°C prior to blocking.
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bioRxiv - Cancer Biology 2023Quote: ... after which heat-induced antigen retrieval was performed in 1x low pH antigen retrieval solution (pH6, Thermo Fisher Scientific). Subsequently ...
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bioRxiv - Neuroscience 2023Quote: ... A mild antigen retrieval step was introduced by immersing the slides into boiled antigen retrieval solution (00-4955-58, Invitrogen) for 10 min ...
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bioRxiv - Physiology 2020Quote: ... Antibodies to the antigen peptide were purified from the final bleed antiserum by affinity-purification using the AminoLink Plus Immobilization Kit (ThermoFisher Scientific, Waltham, MA, USA), with bound antibodies eluted using glycine elution buffer [6.3 ml of 100 mM glycine (VWR Chemicals ...
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bioRxiv - Molecular Biology 2020Quote: ... driven by the human cytomegalovirus (CMV) immediate early enhancer and promoter was assembled using the GeneArt CRISPR Nuclease Vector system (Thermo Fisher Scientific, Waltham, MA), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... antigens were directly coated to Nunc-absorb (ThermoFisher) plates overnight at 4°C or captured using streptavidin ...
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bioRxiv - Immunology 2023Quote: ... Antigen printed pads were blocked with SuperBlock (ThermoFisher) for 1 hour before probed with serum samples (diluted at 1:100 in SuperBlock) ...
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bioRxiv - Physiology 2024Quote: ... heated in antigen retrieval solution (Thermo Fisher Scientific) at 100 °C for 20 min ...
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bioRxiv - Microbiology 2019Quote: ... 4 × 105 mouse splenocytes/well were plated with either with 5 μg/ml YFV-17D NS3 ATLTYRML peptide antigen (57) or with 50 μg/ml of total Vero E6 cellular antigen in RPMI 1640 medium (Gibco, Belgium) supplemented with 10% fetal bovine serum ...
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bioRxiv - Immunology 2023Quote: NY-BR-1 antigen positive cell line CAMA-1 A2B2M and antigen negative SKMEL28 A2B2M were stained with CellTracker™ Deep Red Dye (Invitrogen, C34565) and seeded at 15000 and 10000 cells/well ...
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bioRxiv - Neuroscience 2024Quote: ... slices underwent an additional antigen retrieval step (10 minutes in a pressure cooker in IHC Antigen Retrieval Solution (Invitrogen: 00-4956-58), followed by cooling in ice for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained for surface antigens in PBS (Gibco) or Brilliant Stain Buffer (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... using 20 μg/mouse of antigen in Alum (Invitrogen)7,8 ...