Labshake search
Citations for Thermo Fisher :
401 - 450 of 5122 citations for Cytomegalovirus Purified Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... The solution was adjusted to 5 µg ml-1 in a total of 100 µl per well diluted antigen and were coated into Pierce™ Nickel Coated Plates (Thermo Fisher Scientific, #15142) at 4°C overnight ...
-
bioRxiv - Immunology 2023Quote: ... from singly sorted antigen-specific memory B cells were RT-PCR amplified using SuperScript III and AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Waltham, MA) under conditions previously described46.
-
bioRxiv - Molecular Biology 2023Quote: ... The desired PCR products were purified from agarose purified and cloned into the pENTR/D-TOPO vector (ThermoFisher Scientific, www.thermofisher.com) and transformed into One Shot TOP10 Chemically Competent Escherichia coli (ThermoFisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: Recombinant trimeric ectodomain of SARS-CoV-2 S protein with two proline mutations (rtS-ecto2P) as a vaccine antigen was produced using a Drosophila expression system (Thermo Fisher Scientific, Grand Island, NY). The protein sequence was modified to remove the furin cleavage site (RRAR to GSAG) ...
-
bioRxiv - Immunology 2020Quote: ... then stained with surface and/or intracellular flow cytometry antibodies specific to antigens of interest using eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set (Thermo Fisher Scientific, Waltham, MA) according to manufacturer protocol ...
-
bioRxiv - Immunology 2024Quote: ... cells were stained following the ThermoFisher “BestProtocols: Staining Intracellular Antigens for Flow Cytometry – Protocol A” using the eBioscienceTM Permeabilization Buffer (ThermoFisher Scientific cat#: 00-8333-56) and eBioscienceTM IC Fixation Buffer (ThermoFisher Scientific cat# ...
-
bioRxiv - Molecular Biology 2020Quote: Total RNA was purified with TRIzol reagent (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... Purified DNA was quantified using Qubit-3 (Invitrogen) and 10-100 ng of total DNA was used for library preparation using the KAPA Hyper Prep kit (KAPABiosystems ...
-
bioRxiv - Genetics 2021Quote: ... RNA was purified using Trizol reagent (15596026, Thermofisher), it was reverse transcribed and dOdc was amplified by qPCR ...
-
bioRxiv - Genetics 2021Quote: ... plasmids were purified using the Purelink kit (Thermofisher). Unless noted otherwise ...
-
bioRxiv - Developmental Biology 2021Quote: ... and purified using the MEGAclear kit (Life Technologies). The targeting donor was designed to replace exon 2 in the wild-type allele with two flanking loxP sequences and two homologous arms ...
-
bioRxiv - Neuroscience 2021Quote: Total RNA was purified by applying Trizol (Invitrogen) to cells according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... and purified using GeneJET Extraction Kit (Thermo Scientific). The purified linearized vector and digested foxA gene were ligated (1:3 molar ratio ...
-
bioRxiv - Genomics 2020Quote: ... Purified labelled RNA was quantified with Qubit (Invitrogen) and 5’-OH ends were repaired with PNK prior to library preparation with the NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible).
-
bioRxiv - Biochemistry 2020Quote: ... Purified DNA was quantified via NanoDrop (Thermo Scientific) and 8 pmol of purified DNA was then used for in vitro transcription with T7 RNA polymerase (New England Biolabs Inc.) ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... RNA was purified using Trizol (Invitrogen, CA, USA). Strand-specific sequencing libraries were prepared using Illumina TrueSeq Stranded mRNA Sample Prep kit and sequenced on an Illumina HiSeq3000 machine (150 bp paired end).
-
bioRxiv - Cell Biology 2019Quote: ... RNA was purified using TRIzol (Cat.#15596026,Invitrogen) and RNeasy mini kit (Cat#74004 ...
-
bioRxiv - Neuroscience 2019Quote: Purified (human) fibrinogen with Alexa 488 (ThermoFisher: F13191) was prepared to a final concentration of 2 mg⋅mL−1 ...
-
bioRxiv - Biochemistry 2019Quote: ... purified and finally measured using Qubit instrument (Invitrogen). Then ...
-
bioRxiv - Molecular Biology 2019Quote: ... Purified RNA was treated with DNaseI (ThermoFisher Scientific), heat inactivated ...
-
bioRxiv - Immunology 2019Quote: ... was purified on nickel-nitrilotriacetic acid resin (Invitrogen) according to the manufactureŕs protocol ...
-
bioRxiv - Plant Biology 2019Quote: ... and purified using DNase1 (TURBO DNase, Ambion, USA). The integrity of RNA (RIN>8.5 ...
-
bioRxiv - Genetics 2019Quote: ... Purified RNA was quantified by Nanodrop (Thermo Fisher). cDNA was synthesized with ~200 ng of purified RNA using 1/4 reactions of SuperScript IV VILO Master Mix with EZ DNase (Invitrogen) ...
-
bioRxiv - Bioengineering 2021Quote: ... purified through a Barnstead NANOpure Diamond (Thermo Scientific) or a Milli-Q® IQ 7000 (Millipore-Sigma ...
-
bioRxiv - Bioengineering 2020Quote: ... and the RNA was purified with TRizol (Ambion). crRNA integrity and purity were assessed with 15% acrylamide Urea-PAGE.
-
bioRxiv - Neuroscience 2021Quote: ... and subsequently purified using the MEGAclearTM Kit (Ambion).
-
bioRxiv - Biochemistry 2020Quote: ... Purified DNA was quantified via NanoDrop (Thermo Scientific) and 8 pmol of purified DNA was then used for in vitro transcription with T7 TranscriptAid kits (Thermo Scientific) ...
-
bioRxiv - Cancer Biology 2020Quote: RNA was purified by either TRIzol extraction (Invitrogen) or RNeasy kits (Qiagen ...
-
bioRxiv - Physiology 2021Quote: ... then RNA was purified with PureLink columns (Invitrogen) and reverse transcribed to cDNA as above.
-
bioRxiv - Microbiology 2020Quote: ... Transcribed sgRNAs were purified with MEGAclear kit (Invitrogen). Quality of sgRNAs were assessed with Bioanalyzer ...
-
bioRxiv - Microbiology 2020Quote: Total RNAs were purified using TRIzol reagent (Invitrogen), according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: ... Purified RNA was quantified using a NanoDrop (ThermoFisher) and 1 µg was converted to cDNA using SuperScript™ III First-Strand Synthesis System (Invitrogen ...
-
bioRxiv - Cell Biology 2021Quote: ... Probes were purified using NucAway Spin Columns (Ambion).
-
bioRxiv - Cell Biology 2021Quote: ... and purified using the MEGAclear Kit (AM1908, Ambion) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... The sgRNAs purified using MEGAclear Kit (ThermoFisher Scientific). The target sequence of the sgRNAs are ...
-
bioRxiv - Molecular Biology 2022Quote: ... Transcripts were purified using MEGAclear Kit (ThermoFisher Scientific).
-
bioRxiv - Bioengineering 2022Quote: ... and purified by MEGAclear Kit (Thermo Fisher Scientific) following manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... Reactions were purified using ExoSap-IT (Thermo Fisher) and then 3 μL of each amplicon was diluted with 32 μL of nuclease-free water before adding 10 μL of assembled Cas9-RNP ...
-
bioRxiv - Genomics 2022Quote: ... The products were purified with ExoSAP treatment (Affymetrix) and by column purification (MinElute PCR Purification Kit ...
-
bioRxiv - Microbiology 2022Quote: ... Purified RNA was rigorously DNased using DNAfree (Ambion) according to the manufacturer’s guidelines prior to synthesis of cDNA using SuperScript III (Invitrogen ...
-
bioRxiv - Molecular Biology 2022Quote: ... Purified RNA was quantified by Qubit (Thermo Fisher) and approximately 150 ng of RNA was subjected to rRNA depletion using the Ribo-Zero Gold rRNA Removal Kit (Yeast ...
-
bioRxiv - Genomics 2022Quote: ... and then purified using BioPrime Purification Module (Invitrogen).
-
bioRxiv - Plant Biology 2021Quote: ... Purified total RNAs were quantified by Qubit (Invitrogen) fluorimeter ...
-
bioRxiv - Immunology 2022Quote: ... purified with CaptureSelect CH1-XL resin (Life Technologies), and followed by size exclusion chromatography (SEC ...
-
bioRxiv - Immunology 2019Quote: ... Purified T cells in complete medium [DMEM (Gibco) supplemented with 10% FBS ...
-
bioRxiv - Molecular Biology 2019Quote: ... RNAs were purified using the MEGAclear kit (Ambion). RNA quality was assessed using a NanoDrop (ThermoScientific ...
-
bioRxiv - Molecular Biology 2019Quote: ... Purified RNA was treated with TURBO DNase (ThermoFisher) then cDNA was synthesized using AMV reverse transcriptase (Promega) ...
-
bioRxiv - Microbiology 2020Quote: ... and then purified using Ni-NTA resin (Invitrogen) followed by protein A agarose.
-
bioRxiv - Genomics 2020Quote: RNA was purified with TRIzol reagent (Life Technologies). 1μg total RNA was primed with random hexamers and reverse-transcribed into cDNA using MultiScribe™ Reverse Transcriptase (Life Technologies) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Purified RNA was quantified by Nanodrop (Thermo Fisher). For RT-qPCR ...