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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for Adenovirus Type 5 Hexon Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... the final dye to protein ratio was typically 5-6 dye molecules per protein molecule as measured with a Nanodrop spectrophotometer (Thermo Fisher Scientific). Cells in suspension culture dishes were labeled with 0.5 nM fluorescent IgE for typically 12-20 hours before imaging.
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bioRxiv - Systems Biology 2019Quote: ... Protein pellets were dissolved in 200 uL 5% SDS solution and protein concentrations were determined using Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2022Quote: ... 4 or 5 ug of appropriate antibodies or IgG control proteins were bound to protein G Dynabeads™ as per manufacturer instructions (Life Technologies). Equal portions of cleared lysates were added to antibody or IgG bound Dynabeads™ and incubated for 3 hours or overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifuging at 12.000 rpm for 5 min at 4°C and protein concentration determined using the Pierce™ BCA Protein Assay (Thermo Scientific 23225). Samples were prepared for SDS-Page in 1× NuPAGE™ LDS Sample Buffer containing 50mM DTT ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were clarified by centrifugation at 17,000 xg for 5 min and total protein was quantified in the supernatant using the BCA protein assay (Thermo Scientific, Rockford, IL). SDS loading buffer (final concentrations of 100 mM Tris pH 6.8 ...
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bioRxiv - Neuroscience 2020Quote: Nuclear fractionation from hippocampus of 30-week old wild-type and R6/1 mice was performed using a Subcellular Protein Fractionation Kit for Tissues (Thermo Fisher Scientific #87790) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... DQ™-collagen type I (Life Technologies/Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2020Quote: ... 2 mg/mL collagenase type II (Gibco) in DMEM for 60 minutes at 37oC with inversion of the tubes to mix every 5 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... supplemented with 0.5% AlbuMAX type II (Gibco), 50 μM hypoxanthine and 2 mM L-glutamine ...
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bioRxiv - Biochemistry 2020Quote: ... 42.000 U Collagenase type 2 (Life technologies) and 23.000 U DNase (Life technologies) ...
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bioRxiv - Physiology 2021Quote: ... 0.025% (w/v) collagenase type IV (Gibco) and 5 mM CaCl2 was added to Leffert perfusion buffer containing 10 mM HEPES ...
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bioRxiv - Cell Biology 2020Quote: ... and type IV collagenase (Gibco,17104-019). Glands were shaken at 37°C for 35 min ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with 0.5% Albumax type II (Gibco), 50 μM hypoxanthine ...
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bioRxiv - Genomics 2019Quote: ... 1000 U/mL collagenase type II (Invitrogen) and 10 mg/mL DNaseI (Roche) ...
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bioRxiv - Cancer Biology 2019Quote: ... and 100U/ml Collagenase Type I (Invitrogen); this was incubated for 45min in rotation at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... A Thermocouple (type K from Fisher Scientific) was used to record temperature to 1 decimal place.
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bioRxiv - Molecular Biology 2019Quote: ... then with collagenase type I (Thermo Fisher) (0.65% ...
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bioRxiv - Cancer Biology 2021Quote: ... and digested with collagenase type I (Gibco). All samples were stained with Zombie Aqua (BioLegend ...
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bioRxiv - Neuroscience 2022Quote: ... Type G glycerol immersion (ThermoFisher, Cat#15336741) were used ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 0.5% AlbuMax type II (Gibco), 50 μM hypoxanthine and 2 mM L-glutamine ...
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bioRxiv - Cell Biology 2023Quote: ... (Collagen type I, Thermofisher, Cat No - 1048301). Images were acquired 48h later and gel contraction was quantified with Image J.
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bioRxiv - Biophysics 2023Quote: ... digested with collagenase (Gibco, Collagenase type II), and stored at 18°C in a solution containing (in mM ...
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bioRxiv - Cell Biology 2023Quote: ... except that collagenase Type IV (17104019; Gibco) was used instead of collagenase H to minimize unspecific digestion of proteins other than collagen ...
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bioRxiv - Bioengineering 2023Quote: ... type 1 (0.4 mg/mL, GIBCO™) for 1 h at 37 °C ...
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bioRxiv - Bioengineering 2023Quote: ... type 1 (0.4 mg/mL, GIBCO™) for 1 h at 37 °C to degrade the collagen for flow cytometry experiments ...
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bioRxiv - Cell Biology 2023Quote: Type 1 rat-tail collagen (Life Technologies) was used at a stock concentration of 3 or 10 mg mL-1 ...
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bioRxiv - Immunology 2024Quote: ... 1 mg/ml collagenase type IV (Gibco), and 0.2 mg/ml DNase (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 0.5% Albumax type II (Gibco), 50 μM hypoxanthine ...
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bioRxiv - Microbiology 2020Quote: ... aliquots were diluted 1:2 and 1:5 in 2M urea solvent and their total protein concentration was measured using a Pierce Bicinchoninic Acid Protein Assay (Thermo Scientific, Waltham, MA) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... coated with Cellmatrix Type I-C (Nitta Gelatin, Osaka, Japan) at 37 °C with 5% CO2 in FluoroBrite DMEM (A1896701, Thermo Fisher Scientific, Massachusetts, USA) supplemented with 10% FBS (FB-1365 ...
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bioRxiv - Neuroscience 2024Quote: Cultured sensory neurons from wild-type and TRESK-/- mice were loaded with 5 μM fura-2/AM (F1221, Invitrogen, ThermoFisher Scientific, Waltham, MA, USA) for 45-60 min at 37°C in culture medium ...
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bioRxiv - Neuroscience 2021Quote: ... with 5 μg primary antibody and 50 uL Pierce Protein G magnetic beads (Thermo Scientific). The primary antibodies used were rabbit anti-EMX2 (HPA065294 ...
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bioRxiv - Immunology 2020Quote: ... An additional 50 µL of 5× eBioscience Cell Stimulation Cocktail with protein transporter inhibitor (ThermoFisher) was added and cells were treated for an additional 4 hr at 37 °C.
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bioRxiv - Molecular Biology 2021Quote: ... together with 5 μl of washed protein G-bound paramagnetic Dynabeads (Life Technologies cat#10003D). Antibody binding of RBP complexes was allowed to proceed at 4°C for the times indicated ...
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bioRxiv - Cancer Biology 2020Quote: ... 7mM NaOH) followed by an incubation for 5 hours with Dynabeads Protein G (Invitrogen, 10004D). Beads were then washed with Wash Buffer (20mM Tris pH=8 ...
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bioRxiv - Biophysics 2020Quote: ... and 5 mL of B-PER™ complete bacterial protein extraction reagent (Thermo Scientific #89821) with 2 mM MgCl2 ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were eluted from beads by boiling for 5 min in LDS sample buffer (ThermoFisher) and the eluates analyzed by Western blot as described above.
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bioRxiv - Cell Biology 2021Quote: For expression of proteins 5 ml of an overnight culture of BL21 DE3 AI (Invitrogen) E ...
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bioRxiv - Cell Biology 2023Quote: ... Reduction of proteins was achieved with 5 mM Tris(2-carboxyethyl)phosphine hydrochloride (Thermo Fisher) for 30 min and samples were subsequently alkylated in 10 mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... 5 µg of RNA was precleared with 25 µl of Protein G Dynabeads (Invitrogen, 10003D) for 30 min at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... samples were incubated with 5 µg anti-FLAG antibody bound to Protein G Dynabeads (Invitrogen).
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bioRxiv - Cell Biology 2023Quote: ... 5 × 10e4 C2C12 cells were electroporated with 7.5 pmol TrueCut Cas9 protein v2 (ThermoFisher Scientific) complexed with 22.5 pmol sgRNA using the Neon system (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µl of PageRuler Unstained or PageRuler (Plus) Prestained Protein Ladder (Thermo Fisher Scientific, USA) and 20 µl of each cell lysate sample (approx ...
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bioRxiv - Cancer Biology 2023Quote: ... Presence of active BMP signalling pathway was detected by an antibody against the phosphorylated form of Smad1/5/9 protein complex (Cell Signalling Technology, Cat#13802S) or pSmad1/5 (Thermo Fisher Scientific, Cat#700047) as indicated in figures ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 μg nuclear proteins or 7.5 μg cytosolic proteins were boiled in loading buffer with reducing reagents (Invitrogen, NuPAGE™ LDS Sample Buffer (4X), NP0007) ...
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bioRxiv - Cell Biology 2020Quote: ... 3 % SDS and 5 mM EDTA) and protein concentrations were determined with a Pierce BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Neuroscience 2022Quote: ... 5–15 µg samples were loaded per well (depending on protein of interest) next to 5 µl pre-stained protein ladder (PageRuler, Thermo Fisher Scientific, Waltham, USA). Proteins were separated by constant current (200 V ...
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bioRxiv - Neuroscience 2022Quote: ... The antibody-protein complexes were then captured by addition of Protein G magnetic Dynabeads (5 μL beads/μg antibody; Thermo Scientific, cat. no. 13424229) followed by further incubation for 1 h at 4°C with end-over-end rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein pellets were subsequently re-solubilized in 40 uL of 5% SDS (v/v) and assayed for protein using the PierceTM BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA). 130-170ug of protein/sample (in a total volume of 33 uL) ...
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bioRxiv - Microbiology 2020Quote: ... pyogenes NCTC 8198T was cultivated at the laboratories of the National Collection of Type Cultures (NCTC, London, UK) on Columbia blood agar (Columbia Agar Base plus 5% horse blood, Thermo Fisher Scientific, Waltham, MA, USA), at 37°C ...