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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for Adenovirus Type 5 Hexon Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Type A2 biological safety cabinet (Thermo Fisher Scientific, Waltham, MA, USA). The fish ventral area was disinfected three times by rubbing with 70% ethanol before incision ...
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bioRxiv - Cancer Biology 2024Quote: ... prostates were harvested and subsequently digested with collagenase type II (Gibco) for 2 hrs at 37 °C ...
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bioRxiv - Immunology 2021Quote: Splenocytes were processed as described in the previous section and stimulated with RBD peptides for 5 hours at 37°C with protein transport inhibitor (Invitrogen) and anti-mouse CD107a-FITC antibody (BioLegend) ...
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bioRxiv - Biochemistry 2020Quote: Protein samples were heated at 95°C for 5 minutes before loading and separated onto SDS-PAGE cassette (Thermo Fisher Scientific Bolt™ 4-12% Bis-Tris Plus Gels ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked in TBST with 5% milk protein and probed with the following antibodies: 1:2000 anti-Ty1 (Invitrogen), then 1:1500 goat anti-mouse IgG-HRP (Dako) ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were boiled at 95°C for 5 min before running on a NuPAGE 4-12% Bis-Tris protein gradient mini gel (Invitrogen) at 200 V for 5 min in MOPS running buffer (Invitrogen ...
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bioRxiv - Biochemistry 2019Quote: ... 2 μg of each antibody was diluted in 30 μl PBS-T and then incubated with 5 μl protein A-coated magnetic beads (30 mg/ml, Dynabeads, 10002D, Invitrogen) for 30 min ...
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bioRxiv - Genetics 2020Quote: ... and 2 μg of plasmid encoding tethered NHA-protein using 5 μl of Lipofectamine 2000 and OPTI-MEM media (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... The sections were then exposed to gold conjugated protein A of 5 nm in diameter (Zymed, now part of the Invitrogen). Sections from uninfected cassava leaves were used as additional controls in the immunolabelling experiments ...
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bioRxiv - Biochemistry 2020Quote: ... 5 DNA and protein concentrations were measured using a NanoDrop ND-2000 UV-visible spectrophotometer (Thermo Fisher Scientific, Nottingham, UK).
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bioRxiv - Neuroscience 2020Quote: We quantified vWA5A and ADAMTS19 protein levels by lysing 5 x 105 cells using Pierce IP Lysis Buffer (Thermo Fisher) following the recommended protocol and measured human vWA5A and ADAMTS19 with high-sensitivity ELISA kits (both from MyBioSource ...
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bioRxiv - Microbiology 2021Quote: ... The samples were centrifuged for 5 min at 10,000 xg and the protein content of the supernatant was determined using a BCAassay kit (Thermo Scientific). The c-di-AMP concentrations are presented as ng c-di-AMP/mg C ...
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bioRxiv - Plant Biology 2021Quote: ... using 5 μgr Diagenode Ab C15410004 H3K9ac antibody and a mix of 25/25 μl of protein-A/G dynabeads (Invitrogen). 5-10 ng of DNA per sample were used for library preparation with Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... samples were boiled for 5 minutes in NuPage LDS sample buffer and run on NuPage Bis-Tris 4-12% protein gels (Invitrogen), and proteins were transferred to PVDF membranes (Immobilon-FL ...
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bioRxiv - Microbiology 2022Quote: ... An equivalent of 0.25 midgut and 5 salivary glands were resolved in a NuPAGE™ 10% Bis-Tris Protein Gel (Invitrogen) under reducing conditions and transferred to a PVDF membrane Invitrogen™ Power Blotter Select Transfer Stacks ...
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bioRxiv - Biochemistry 2022Quote: ... 550 pmol of protein was then added to a tube containing 1.1 nmol of 5’Biotin(GGTTAG)2 DNA (Thermo Fisher) in a volume of 100 µL dilution buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... Protein samples were heated at 95°C for 5 minutes before loading and separated onto SDS-PAGE cassette (Thermo Fisher Scientific Bolt™ 4-12% Bis-Tris Plus Gels ...
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bioRxiv - Plant Biology 2021Quote: ... 5 mM MgCl2 and 0.5 mM MnCl2) and quantified by using Pierce™ BCA Protein Assay Kit (23227, Thermo scientific). In the case of protease digestions ...
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bioRxiv - Plant Biology 2019Quote: ... and then rotated for another 5 min before loading the strip into the well of a NuPAGE 4-12% Bis-Tris protein gel (Invitrogen).
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bioRxiv - Microbiology 2019Quote: ... Beads were then washed with ice-cold lysis buffer 3 times for 5 minutes and proteins were recovered by boiling in denaturing loading buffer (Invitrogen).
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bioRxiv - Physiology 2019Quote: ... Sulfhydrated proteins in the dried bands were reduced with 0.1 mL of 65 mM dithiothreitol (DTT) (Fisher Scientific # BP172-5) at room temperature for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Cas9/sgRNA RNP complex was assembled just prior to injection and after 5 min incubation at room temperature 200 pg of Cas9 protein (Invitrogen) and 100 pg of sgRNA were co-injected into one-cell stage Tg(fli1ep:Lifeact-EGFP)zf495;Tg(kdr-l:ras-mCherry)s916 embryo ...
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bioRxiv - Biochemistry 2021Quote: ... Pellets were dried for 20 min at room temperature then resuspended in 5 μL of 1 M Tris and 10 μL of protein loading buffer (2x NuPAGE LDS sample buffer (Invitrogen), 715 mM 2-mercaptoethanol) ...
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bioRxiv - Biochemistry 2021Quote: 24.5 μL of 5 μM solution of every protein was prepared in 96-well plate MicroAmp Optical plate (Applied biosystems), and incubated at room temp ...
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bioRxiv - Genomics 2021Quote: ... 10 µg of antibody (3 µg for H3K27ac) was added to 5 µg of sonicated chromatin along with Dynabeads Protein A magnetic beads (Invitrogen) and incubated at 4 °C overnight ...
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bioRxiv - Neuroscience 2021Quote: ... pH 6.8) for 5 min at 95° C and loaded to NuPAGE 4-12% Bis-Tris Protein polyacrylamide gels (Invitrogen, #NP0323BOX). After electrophoresis ...
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bioRxiv - Immunology 2020Quote: ... then incubated another 5 hr at 37 C with additional Protein Transport Inhibitor Cocktail (eBioscience 00-4980-03, Thermo Fisher). Cells were then fixed and permeabilized with BD Cytofix/Cytoperm Kit (BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... 5 mg of total protein/sample was combined with 330 μL RIPA-equilibrated PierceTM Streptavidin Magnetic Beads (Thermo Fisher Scientific) and incubated on a rotator at 4°C overnight ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 µg of antibody (3 µg for H3K27ac) was added to 5 µg of sonicated chromatin along with Dynabeads Protein A magnetic beads (Invitrogen) and incubated at 4 °C overnight ...
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Allelic diversity uncovers protein domains contributing to the emergence of antimicrobial resistancebioRxiv - Microbiology 2022Quote: ... Samples were diluted 1:5 in sample loading buffer and quantified using the Pierce BCA Protein Assay Kit (Thermo Scientific). Lysates were normalized to 100μg/mL in 1X Bolt™ LDS Sample Buffer (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were centrifuged (13,000 × g for 5 min at 4°C) and supernatants are precleared with 10 μl protein G beads (Invitrogen; 1004D) at 4°C for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... 20 three months old transgenic TgN3R182C150 mice were immunized initially either with 25 µg of aggregated NOTCH3 EGF1-5 protein (n=11, vaccinated) plus adjuvant (Imject Alum Adjuvant, ThermoFisher) or PBS (n=9 ...
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bioRxiv - Microbiology 2023Quote: ... were coated with 100 µL of recombinant SARS-CoV-2 S protein (Wuhan-1 strain and BA.5) at a concentration of 1 µg/mL in PBS (Gibco) at 4°C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... and supernatants were incubated (2 h, 4°C) with 5 µg antibodies conjugated to 25 µL protein G dynabeads (Invitrogen). Samples were immobilised ...
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bioRxiv - Neuroscience 2023Quote: ... the proteins were separated by a short electrophoresis migration (5 min) at 200 V on with MES/SDS 1X (Invitrogen) as running buffer ...
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bioRxiv - Molecular Biology 2023Quote: Thermal shift assays were carried out using 4 μM PhPINK1 (residues 115–575) and 5× SYPRO Orange Protein Gel Stain (Invitrogen) in 25 mM Tris (pH 8.5) ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were digested by incubating gel pieces with 25 mM Triethylammonium bicarbonate (TEAB) containing 5 μg/mL Trypsin (ThermoFisher #90058), at 30°C ...
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bioRxiv - Plant Biology 2023Quote: ... and then rotated for another 5 min before loading the strip into the well of a NuPAGE 4-12% Bis-Tris protein gel (Invitrogen).
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bioRxiv - Biochemistry 2023Quote: ... Mitochondrial extracts were centrifuged at 18’000 rcf for 5 min and protein concentrations were determined by BCA assay (Thermo Fisher). 180 μg of mitochondrial proteins were subjected to a click reaction using a commercial kit (Click-iT Cell Reaction Buffer Kit ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins in the gel fragments were reduced using 5 mM Bond-Breaker TCEP solution (Thermo Scientific, Product No. 77720), alkylated using 100 mM iodacetamide (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: The sonicated samples (approximately 5 µg chromatin/sample) were cleared for 2 hours using non-coupled magnetic beads (Protein G Dynabeads, Invitrogen), then further mixed with 5 µL antibody (anti-H3K27me3 ...
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bioRxiv - Biophysics 2023Quote: ... denatured by heating for 5 min at 95°C and subjected to electrophoresis using precast NuPAGE Novex 4–12% Bis-Tris Proteins Gels (Invitrogen) in NuPAGE MES SDS Running Buffer (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were suspended in 710 µl of RIPA buffer with 7.5 µl PIC and incubated with gentle mixing overnight at 4 °C with 20 µl of DynabeadsTM Protein G (Invitrogen) coated with antibody against the analysed protein (listed in Supplementary Table S2) ...
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bioRxiv - Molecular Biology 2023Quote: Recombinant Rpb4/Rpb7-6His protein (5 μg) was incubated in a 50 μL slurry of HisPur Cobalt Resin (Thermo Scientific) in binding buffer (20 mM Tris [pH 7.5] and 50 mM NaCl ...
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bioRxiv - Molecular Biology 2023Quote: Protein (2 µg total lysate, 5 µg soluble lysate) was loaded onto 4-12% Bis-Tris NuPAGE minigels (Invitrogen, NP0322BOX). 10 µL of precision plus protein kaleidoscope prestained standard (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... 0.5 mls of Sm/RNPs (0.75 mg/mL) were labeled using the Alexa Fluor 647 Protein Labeling Kit (Invitrogen A20173) following the manufacturer’s instructions without removal of unbound AF647.
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bioRxiv - Cancer Biology 2024Quote: ... Between 10 to 30 µg of protein were prepared in Laemmli buffer boiled for 5 min and proteins size separated by SDS-PAGE using NuPAGE 10% Bis-Tris 1.5 mm gels (Life Technologies). Proteins were then transferred onto nitrocellulose membranes (Life Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... A total of 5 μg of GFP antibody that was pre-mixed in a 50 uL volume of Dynabeads protein A (Invitrogen) was added to each sonicated chromatin sample with 1% Triton X-100 and incubated overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... Cas9-gRNA RNPs were prepared by incubating either 0.5 µl of each gRNA with 1.5 µl TrueCut Cas9 Protein v2 (5 µg/µl, Thermo Fisher Scientific) or 1 µl of each gRNA with 1.5 µl recombinant Cas9-NLS (5 µg/µl ...
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bioRxiv - Immunology 2024Quote: ... C57Bl/6J one-cell zygotes were electroporated (Nepa21, Sonidel) using the 5 mm electrode (CUY505P5) with 333 ng/µl Cas9 (TrueCut spCas9 protein V2, Invitrogen) and 75 ng/µl guide RNA (Synthego) ...