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Citations for Thermo Fisher :
51 - 100 of 10000+ citations for Adenovirus Type 5 Hexon Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... alongside 5 μl PageRuler Prestained Protein Ladder (Thermo Scientific), and electrophoresed at 100-200 V for one to two hours in a Mini-PROTEAN Tetra Vertical Electrophoresis Cell (BIORAD) ...
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bioRxiv - Microbiology 2019Quote: ... Protein was incubated with 5 × SYPRO orange (Thermo Scientific) for 10 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... using the BenchMark protein ladder (Invitrogen, 5 μL/lane) as a standard ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were reduced with 5 mM TCEP (Thermo Scientific) and subsequently alkylated with 10 mM iodoacetamide (Sigma) ...
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bioRxiv - Cancer Biology 2022Quote: 1 µl Cas9 protein (5 µg/µl, Thermo Fisher) and 100 pM of synthetic guide RNA (Tspan8 guide sequence 5’ – 3’ ...
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bioRxiv - Molecular Biology 2022Quote: ... 5% protein-free hybridoma medium (PFHM-II, Thermo Fisher), 2 mM L-glutamine (Thermo Fisher) ...
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bioRxiv - Plant Biology 2023Quote: ... using 5 µl PageRuler™ Prestained Protein Ladder (ThermoFisher) as marker ...
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bioRxiv - Cancer Biology 2021Quote: ... p53-associated protein were pulled down by 5 μl DynabeadsTM protein G beads (#10004D, Invitrogen) at 4 °C for 1-1.5 h ...
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bioRxiv - Genomics 2020Quote: ... 5 µg antibody was coupled to 50 µl Protein A or Protein G Dynabeads (Invitrogen). The following antibodies were used ...
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bioRxiv - Genomics 2022Quote: ... 5 μg of antibody bound to 50 μl protein A or protein G Dynabeads (Invitrogen) was incubated with soluble chromatin overnight at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... rProprotein convertase subtilisin/kexin type 5 (PCSK5) was expressed in the ExpiCHO expression system produced by Gibco™ ...
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bioRxiv - Neuroscience 2022Quote: ... Type IV (ThermoFisher) for 1 hr and dissociation into smaller pieces through manual tritulation before seeding onto feeder layer of MEFs.
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bioRxiv - Physiology 2020Quote: ... The hairpin template oligonucleotides were synthesized by Integrated DNA Technologies and were subsequently cloned into the adenovirus vector of the pAD/Block-it system (Invitrogen) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2020Quote: ... Promoter and insert were inserted into the E1 region of adenovirus (Ad5ΔE1/E3) backbone plasmid pAd/PL-DEST through Gateway recombination following the manufacturer’s instructions (Gateway System; Invitrogen, Karlsruhe, Germany). Adeno virus stocks were titrated using the cytopathic effect in HepG2 cells as previously described58.
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bioRxiv - Cell Biology 2023Quote: Generation of replication-defective recombinant adenovirus expressing N-SREBPs under CMV promoter was performed using ViraPower Adenoviral Expression System (#K4930-00, Invitrogen) as described earlier (54) ...
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bioRxiv - Biophysics 2023Quote: ... and untagged Pro283 NP were used to produce adenovirus according to the manufacturer’s instructions (Life Technologies ViraPower Adenoviral Expression System). NP-encoding sequence was cloned from plasmid HDM_FLAG_Aichi68_NP_IRES_mCherry.
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bioRxiv - Biochemistry 2023Quote: ... wild-type α-actin and 15 μM Pi-sensor (MDCC-labeled phosphate binding protein, Thermo Fisher Scientific) was prepared ...
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bioRxiv - Bioengineering 2023Quote: ... / type-I collagen (Gibco™ collagen type I, rat tail, ThermoFisher) matrix with a 1:1:2 volume ratio ...
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bioRxiv - Genomics 2020Quote: ... 5 μl of protein G Dynabeads (Invitrogen, catalog no. 10003D) slurry and 5 μl of anti-rabbit Dynabeads (Invitrogen ...
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bioRxiv - Genomics 2022Quote: 5 μl of Dynabeads Protein A (10002D, Thermo Fisher Scientific) were used in each MOWChIP assay ...
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bioRxiv - Genetics 2023Quote: ... containing 5% Protein Free Hybridoma Medium (PFHM II; ThermoFisher Scientific). On day 0 ...
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bioRxiv - Immunology 2024Quote: ... alongside 5 µl of protein ladder (26619, ThermoFisher Scientific, UK). Gel electrophoresis was performed at 120 V for 1 hour 30 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... YSM tissues from day 5 embryos were treated with collagenase (collagenase type 4, 17104019, ThermoFisher, Waltham, MA, USA) to partially digest the extracellular matrix and facilitate cell isolation [17] ...
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bioRxiv - Developmental Biology 2022Quote: ... The sliced tissue was then transferred to a glass tube along with a magnetic stir bar and 1.5 ml digestion buffer in Dulbecco’s modified Eagle’s medium containing collagenase type II (250 U/ml) (17101015; Gibco), collagenase type IV (300 U/ml ...
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bioRxiv - Microbiology 2021Quote: RNA was isolated from HepG2.2.15 cells infected with adenovirus (pAd-GRP78 or pAd-GFP) using TRIzol reagent (Invitrogen, The United States) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 S gene containing plasmid p20017 and adenovirus backbone plasmid pAADV-C01 (Genemedi, China) were co-transfected into HEK293 based adapted viral production cell (ThermoFisher, USA). Viral production cells were seeded in a 6 well TC treated plate (Nest ...
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bioRxiv - Cell Biology 2022Quote: ... according to the manufacturer’s instructions using ‘turbo-type’ green fluorescent protein (tGFP) mRNA (# 88880 Thermo Fisher Scientific Inc.) as a template for translation ...
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bioRxiv - Pathology 2020Quote: ... Fractions were tested for protein concentration using 5 μL of protein sample mixed with 10 μL Coommassie Protein Assay reagent (Thermo Scientific). Pooled fractions were concentrated to 4 mL using 10 kD molecular weight cut-off spin columns (Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... Fractions were tested for protein concentration using 5 μL of protein sample mixed with 10 μL Coomassie Protein Assay reagent (ThermoFisher Scientific). Pooled fractions were concentrated to 4 mL using 10 kDa molecular weight cut-off spin columns (Millipore ...
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bioRxiv - Cell Biology 2019Quote: ... using 3–5 µg of primary antibody against each protein and protein-G magnetic Dynabeads (10003D, Invitrogen) and incubated overnight at 4°C on rotation ...
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bioRxiv - Molecular Biology 2021Quote: ... The fragmented chromatin was then pre-cleared with 5 μl of Dynabeads Protein A/Protein G (ThermoFisher) and immunoprecipitated overnight at 4 °C with the pre-prepared antibody-beads complex ...
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bioRxiv - Microbiology 2023Quote: ... and at 5 mg/mL total viral protein by Qubit Protein Assay quantification kit (Life Technologies, Q33211).
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bioRxiv - Bioengineering 2023Quote: ... / type-I collagen (Gibco™ collagen type I ...
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bioRxiv - Cell Biology 2023Quote: ... type I (ThermoFisher, #A1048301); Fibronectin ...
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bioRxiv - Cell Biology 2020Quote: ... Production and expression of adenovirus encoding mCherry/mCherry-KIF4A was performed using pAd/CMV/V5-DEST Gateway Vector Kit (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... 5% glycerol) with 1% Halt protein/phosphatase inhibitor (ThermoFisher Scientific; 78446). Samples were spun at 17,100xg for 15 minutes at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... buffer alongside PageRuler Protein Ladder (5 µL/lane; Thermo Scientific, 26616) and run in pre-chilled 1X MOPS Buffer (Thermo ...
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bioRxiv - Biophysics 2022Quote: ... The dialyzed protein containing 5% Acetonitrile (Fisher Scientific, Hampton, NH, USA) and 0.1% TFA was injected to the pre-equilibrated column and eluted with a 10 min gradient to 80% acetonitrile/0.1% TFA ...
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bioRxiv - Biophysics 2022Quote: ... The protein solution was supplemented with 5 mM ATP (Thermo Fisher) as well as 5 mM Phosphoenolpyruvic acid (Alfa Aesar ...
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bioRxiv - Immunology 2021Quote: Pierce Protein A/G Ultralink Resin (5 µL; Thermo Fisher Scientific) and 1 µL sample serum in 100 µL Buffer A (50 mM Tris ...
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bioRxiv - Immunology 2022Quote: ... including 5 mg protein and 5000× SYPRO Orange (Invitrogen, Carlsbad, USA) as fluorescence probe ...
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bioRxiv - Cell Biology 2023Quote: ... 4-5 μl Page Ruler Pre-stained Protein Ladder (Thermo Scientific), and 10 μl of 4X Dye (last three grooves ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5 ng/uL human IL-6 recombinant protein (ThermoFisher PHC0066).
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bioRxiv - Cancer Biology 2021Quote: ... and SK-N-SH cell lines were purchased from the American Type Culture Collection (www.atcc.org) and grown in a humidified chamber with 5% CO2 in RPMI 1640 medium (Gibco, #11875119) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Neuroscience 2024Quote: Cultured sensory neurons from wild-type and TRESK-/- mice were loaded with 5 μM fura-2/AM (F1221, Invitrogen, ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... in 6 well plates were transiently transfected with 2μg of either wild-type or mutant SARS-CoV-2 S protein plasmid with Lipofectamine 3000 (Invitrogen) at a ratio of 1:2:2 DNA ...
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bioRxiv - Developmental Biology 2022Quote: ... we isolated total protein from 45 dpf adad1Y67X mutant and wild-type testes in RIPA buffer (Thermo Fisher Scientific). After denaturation through heat and 100 mM DTT ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins in the clear supernatant (protein extract) were cross-linked with 5 mM DSP (Thermo Fisher Scientific Pierce) for 2 h with agitation ...
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bioRxiv - Bioengineering 2023Quote: Protein samples (5 µg protein) were mixed with either 4 x LDS sample buffer (Thermo-Fisher Life Technologies) or 4x LDS sample buffer + 50 mM dithiothreitol for SDS-PAGE analysis under non-reducing or reducing conditions ...
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bioRxiv - Immunology 2021Quote: Thymi from 4-week-old wild type mice were harvested and 5 million thymocytes per condition were cultured in IMDM medium (Gibco) with 2% FBS (HyClone Thermo Fischer Scientific ...