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Citations for Thermo Fisher :
2201 - 2250 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: Western blot analysis was performed using an anti-His antibody (Invitrogen, LOT 1902132) to confirm the identity of splicing and cleavage products ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS, Thermo Fisher Scientific) and 1% Pen-Strep (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... plates were coated with 1 μg/mL 6x-His tag polyclonal antibody (Invitrogen) in 1x PBS for 6 hours at ambient temperature and blocked overnight with 0.5% NCS/5% Goat Serum/5% Milk/0.2% PBS-T ...
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bioRxiv - Immunology 2021Quote: ... or left unstimulated in 10% HI-FBS RPMI-1640 (ThermoFisher Scientific cat. 11875085) containing Brefeldin A (eBioscience cat ...
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bioRxiv - Microbiology 2021Quote: ... gingivalis were stained with 15 mg/l hexidium iodide (HI; Thermo Fisher Scientific) and 4 mg/l 5-(and-6)-carboxyfluorescein and succinimidyl ester (FITC ...
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bioRxiv - Cell Biology 2021Quote: ... before being incubated with the primary antibody (6x-His Tag Monoclonal Antibody, Invitrogen) overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... and His-tagged mCherry (strain He/80/FR) were synthesized by Thermo Fisher Scientific (Waltham ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tagged protein was purified using HisPur Cobalt Resin (Thermo Fisher Scientific) in 20 mM Hepes (pH 7.3) ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (hi-FBS, Gibco, 16140-071), 100U/mL Penicillin/100ug/mL Streptomycin (pen/strep ...
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bioRxiv - Plant Biology 2022Quote: ... the secondary antibody used was anti-6x-His tag monoclonal (Invitrogen, # MA1-21315) with 1:100 dilutions ...
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bioRxiv - Plant Biology 2022Quote: ... The supernatant was then purified using Ni-NTA His affinity agarose (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... 6x-His Epitope Tag Monoclonal Antibody (#MA1-21315, Thermofisher Scientific; dilution 1:500) was used for His-tag staining of the TRPA1 channel in fixed cells for 1 h at RT ...
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bioRxiv - Neuroscience 2022Quote: ... each experiment sample was separated on a Hi-pH column (Thermo Fisher Scientific) according to the vendor’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... recombinant proteins were detected using primary anti-His antibody (ThermoFisher, Cat. # PA1-983B) and an anti-RNAP antibody (BioLegend ...
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bioRxiv - Synthetic Biology 2024Quote: ... then diluted at 1:100 in Hi-Def Azure Media (Fisher Scientific # 3H5000) supplemented with 1% Glucose and various concentrations of peptide in 100 µl in a 96well plate ...
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bioRxiv - Plant Biology 2024Quote: ... synthesized GUN1-FL protein was purified using His-Tag Dynabeads™ (Invitrogen; 10103D) and confirmed by Western blotting using His-tag antibody (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... Bio-His-FIKK13 was incubated with different quantities of magnetic streptavidin beads (Invitrogen) for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... at a 1:5,000 dilution and mouse anti-His monoclonal antibody (Thermo Fisher Scientific or Abcam ab216773 ...
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bioRxiv - Cell Biology 2024Quote: ... excluding the HI-FBS and supplementing with 0.1% BSA (Life Technologies 15260-037). CHIM was used for the experiments in Figures 4 ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 μl of Dynabeads™ His-Tag Isolation and Pulldown beads (ThermoFisher 10104D) for each CFPS reaction were equilibrated twice in equal volumes of wash buffer before addition to the purified reactions to remove the Ulp-protease and incubated with spinning at 4 °C for at least 30 minutes ...
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bioRxiv - Synthetic Biology 2023Quote: ... Membranes were then incubated with an anti-His antibody with HRP conjugate (Invitrogen) in 1% BSA at 4°C overnight with gentle rocking ...
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bioRxiv - Cancer Biology 2023Quote: ... neutralized by adding RPMI-1640 with 10% heat inactivated FBS (HI FBS, Gibco), and spun down at 200 x g for 10 minutes to pellet the cells ...
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bioRxiv - Plant Biology 2023Quote: ... Immunoblotting was performed using anti-His antibody (Thermo Fisher, Cat. No. MA1-21315) at a dilution of 1:10,000 ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 10% (v/v) heat-inactivated foetal calf serum (hi-FCS) (Gibco) and 1% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... Western blots were carried out probed with anti-His mAb (1:1000, Invitrogen) followed by anti-mice IgG-HRP (1:20000 ...
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bioRxiv - Cancer Biology 2022Quote: ... medium containing 10% (v/v) heat inactivated fetal calf serum (HI-FCS; Gibco), 100 U/mL Penicillin (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... The His-tagged protein was purified using HisPur Cobalt Resin (Thermo Fisher Scientific) in 20 mM Hepes (pH 7.3) ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10 % v/v heat-inactivated fetal calf serum (HI-FCS, Gibco), 1 mM non-essential amino acids (NEA ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 1% (v/v) HI-FBS and N-2 supplement (Gibco™). ATM knockout (KO ...
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bioRxiv - Genetics 2023Quote: ... Libraries were mixed with Novex 5X Hi-Density TBE Sample Loading Dye (Thermofisher), then loaded into a 6% TBE acrylamide gel with the Quick-Load pBR322 DNA-MspI Digest ladder and run for 65 minutes at 120 V ...
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bioRxiv - Biochemistry 2023Quote: ... Successful binding was analyzed using anti-His Tag antibody (PCRD, Invitrogen #MA1-135) or anti-Myc Tag (DCRD ...
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bioRxiv - Developmental Biology 2024Quote: Primary antibodies used were HRP-conjugated anti-His (Invitrogen 46-1009; 1:2000) and mouse anti-FLAG (Sigma F1804 ...
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bioRxiv - Molecular Biology 2024Quote: ... MBP-His-TEV-Epe1 was cloned into a pFastBac vector (Thermo Fisher Scientific) and used for Bacmid generation ...
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bioRxiv - Cell Biology 2024Quote: ... His-tagged proteins were subsequently purified using Ni-NTA–agarose beads (Thermo Scientific) and elution buffer (20 mM HEPES ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cDNA plasmids in pcDNA™4/TO Mammalian Expression Vector were transiently transfected into Expi293F™ cells stably expressing human FHF2b and human SCN1B subunit (polyclonal) background using ExpiFectamine™ 293 Transfection Kits (Gibco,Thermo Fisher Scientific CAT #: A14524). Induction was achieved using Tetracycline (Sigma Aldrich) ...
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bioRxiv - Microbiology 2020Quote: Human ileocecal adenocarcinoma HCT-8 cells (ATCC) and THP-1 cells (ATCC) were maintained in Advanced RPMI 1640 (ThermoFisher Scientific) supplemented with 10% fetal bovine serum and 1X penicillin-streptomycin (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: Total or individual CD8+ or CD4+ T cells were purified from PBMC suspensions from our PLWH and HIV-1 negative control study cohorts by negative immunomagnetic selection (purity >90%) using the Untouched Human T cell and CD4 T cell Dynabeads Kits (Invitrogen) and the MojoSort Human CD8 T cell (BioLegend ...
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bioRxiv - Biochemistry 2019Quote: U-2 OS cells (human bone osteosarcoma epithelial cells, ATCC Number: HTB-96 [17]) were grown in D-MEM Glutamax (Gibco) supplemented with final concentrations of 10% fetal calf serum (Invitrogen) ...
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bioRxiv - Cancer Biology 2019Quote: ... and CD19+ B-cells were isolated by negative selection using the Dynabeads® Untouched™ Human B Cells kit (Invitrogen). Mantle cell lines (Z-138 ...
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bioRxiv - Bioengineering 2021Quote: All experiments used a previously described research cell bank of T-REx-293 human embryonic kidney (HEK) cells stably expressing the tetracycline repressor protein (ThermoFisher) (4) ...
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bioRxiv - Cancer Biology 2021Quote: ... Virus was produced in 293T cells and human melanoma cell lines were infected and selected with 1 μg/mL Puromycin (Gibco) for 3 days ...
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bioRxiv - Biochemistry 2021Quote: The knock-out cell line (GTPBP5KO) was generated in the Flp-In T-Rex human embryonic kidney 293 (HEK293T) cell line (Invitrogen) using CRISPR/Cas9 technology targeted on exon 1 of MTG2 gene ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was isolated from human cells (1 x 106 cells) using the TRIZOL reagent according to the manufacturer’s instructions (ThermoFisher Scientific). RNA integrity for each sample was confirmed with by Nano Drop ...
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bioRxiv - Cell Biology 2021Quote: ... RWPE-1 (human normal prostate epithelial cells, CRL-11609, ATCC) cells were cultured in Keratinocyte Serum Free Medium (17005042, Gibco) supplemented with 0.05 mg/ml bovine pituitary extract ...
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bioRxiv - Microbiology 2022Quote: To evaluate expression levels of A24Rwt and A24Rcd, human 293T cells (105 cells, 12-well plates, triplicates) were transiently transfected using Lipofectamine 2000 (LPF2000, Invitrogen) with 1 µg of pCAGGS expression plasmids encoding C-terminal HA-tagged A24Rwt or A24Rcd ...
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bioRxiv - Immunology 2022Quote: Human bronchial epithelial cells (BEAS-2B cells, ATCC® CRL-9609™) were cultured in LHC-9 medium (Life Technologies) and human lung fibroblast (MRC-5 cells ...
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bioRxiv - Genomics 2019Quote: Hand-picked human pancreatic islets were dissociated to single cells using enzyme-free Hanks’-based Cell Dissociation Buffer (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... cells and human mesenchymal stem cells were grown in DMEM culture medium supplemented with 10% fetal bovine serum (FBS) (Gibco), 100 mg/ml streptomycin and 100 units/ml penicillin (Gibco ...
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bioRxiv - Cell Biology 2020Quote: Freshly isolated HTII-280+ and HTII-280− cells derived from CD31−CD45−EpCAM+ cells of human distal lungs were resuspended in Advanced DMEM/F12 (Thermofisher) base medium supplemented with 10 mM Hepes (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... immortalized human lung fibroblast cells and HCT116 colorectal cancer cells were cultured in a 1:1 mixture of DMEM (Gibco) and Ham’s F10 (Invitrogen ...