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Citations for Thermo Fisher :
2351 - 2400 of 10000+ citations for Glutathione S Transferase GST Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and 100 µg/mL Streptomycin (P/S) (Gibco, Life Technologies). Cells were maintained at 37°C ...
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bioRxiv - Immunology 2023Quote: ... blasticidin S-HCl and trifluoroacetic acid were purchased from ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: Expi-CHO-S Cells (Cat# A29127; ThermoFisher Scientific, Waltham MA) were cultured in ExpiCHO Expression Medium (Cat# A2910001 ...
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bioRxiv - Microbiology 2023Quote: ... and 100 mg/ml penicillin and streptomycin (P/S; Gibco) (complete DMEM) ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated in calcium-free Caco2 medium (S-MEM; Gibco), supplemented with glutamine ...
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bioRxiv - Synthetic Biology 2024Quote: CHO-S cell lines (Thermo Fisher Scientific, Waltham, MA USA) were cultivated in disposable 125-mL polycarbonate shake flasks with a working volume of 30 mL ...
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bioRxiv - Cell Biology 2024Quote: ... and 100 µg/ml streptomycin (P/S, Thermo Fisher Scientific). Long bone osteoblasts were isolated from femora and tibiae of 8-week-old mice ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed with S-MEM (Thermo Fisher Scientific, #11380037) with 2% heat-inactivated fetal bovine serum (HI-FBS ...
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bioRxiv - Microbiology 2019Quote: ... PCR amplicons were cleaned and normalized using SequalPrep Plate Normalization Kit (Invitrogen, Carlsbad, CA, USA) and combined into four pools ...
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bioRxiv - Microbiology 2022Quote: ... The amplicons were normalized using a SequalPrep™ Normalization Plate Kit (Thermo Fisher Scientific Inc.) and multiplexed.
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bioRxiv - Microbiology 2019Quote: ... Plates were scraped and transformant plasmids isolated using the GeneJET plasmid purification kit (Thermo scientific).
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bioRxiv - Microbiology 2022Quote: ... Sample normalization of the indexed amplicons was performed with the SequalPrep normalization plate kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... and successfully amplified libraries were normalized using SequalPrep™ Normalization Plate Kit (Thermo Fisher, USA). Normalized amplicons were then pooled and concentrated 20:1 using the 50K Dalton Millipore filters (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were cleaned and normalized using the SequalPrep Normalization Plate Kit (Invitrogen, Massachusetts, USA) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... ELISA plates (96-well plates, Immunol4HBX, Thermo Fisher) were coated with a 50/50 mixture of His-Spy-RBD and RBD-Spy-His (2 μg/mL ...
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bioRxiv - Microbiology 2019Quote: ... SAB agar plates (5-cm plates, Fisher Scientific) inoculated with 200 μL of a M ...
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bioRxiv - Bioengineering 2020Quote: 96-well plates (Nunc MediSorp plates; Thermo Scientific) were coated overnight at 4 °C with 50 ng/well of purified antigen (recombinant RSVF or designed immunogens ...
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bioRxiv - Bioengineering 2020Quote: 96-well plates (Nunc MediSorp plates; Thermo Scientific) were coated overnight at 4 °C with 50 ng/well of purified antigen (recombinant RSVF or designed immunogens ...
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bioRxiv - Immunology 2020Quote: ... Maxisorb 96-well plates (Nunc-immuno plates, Dutscher) were coated overnight at room temperature (RT ...
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bioRxiv - Immunology 2022Quote: ... Nunc Immuno Plate MaxiSorp plates (Thermo Fisher Scientific) were coated either with 50 μg/ml NP4-BSA or 50 μg/ml NP26-BSA and incubated overnight at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... Custom PCR plates (TaqMan 96 well-plate, ThermoFisher) were used to assess mRNA expression on a QuantStudio 12K Flex Real-Time PCR System (ThermoFisher ...
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Micro topographical instruction of bacterial attachment, biofilm formation and in vivo host responsebioRxiv - Microbiology 2022Quote: ... The viability of attached cells was evaluated by fluorescent staining with the LIVE/DEAD® BacLight™ bacterial viability kit (Molecular Probes, Life Technologies).
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Micro topographical instruction of bacterial attachment, biofilm formation and in vivo host responsebioRxiv - Microbiology 2022Quote: ... The viability of attached cells was evaluated by fluorescent staining with the LIVE/DEAD® BacLight™ bacterial viability kit (Molecular Probes, Life Technologies).
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bioRxiv - Synthetic Biology 2020Quote: Ten percent of the final RNA amount was used for chemical labelling with Cy3 fluorescent dye using Arcturus Turbo Labeling Cy3 Kit (ThermoFisher, Applied Biosystems, Cat No. KIT0609). The chemical labelling procedure and subsequent removal of free Cy3 dye was performed following manufacturer’s protocol with 15 μg of RNA per reaction ...
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bioRxiv - Developmental Biology 2023Quote: ... EdU-labelled cells were fluorescently marked by click-it chemistry with the specific addition of a fluorescent azide on EdU molecules (Click-iT™ EdU Cell Proliferation Kit, 488 or 555 dye, ThermoFisher, #C10337 and #C10638), following (Demilly et al. ...
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bioRxiv - Cell Biology 2024Quote: ... all libraries were quantified using the highly sensitive fluorescent dye-based Qubit® ds DNA HS Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) and sequencing was performed using the illumina NovaSeq 6000 system (Illumina ...
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bioRxiv - Biophysics 2020Quote: ... mouse cell line was obtained from ATCC and cultured in Dulbecco’s modified Eagle medium (DMEM) (Wisent) supplemented with 10% fetal bovine serum (FBS) (Wisent) and 1% Penicillin-Streptomycin antibiotic (P/S) (Thermo Fisher). The cells were incubated at 37 °C in 5% CO2 environment and ...
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bioRxiv - Biochemistry 2021Quote: ... blotted for 1–2 s and plunge frozen into a liquid ethane/propane mixture with a Vitrobot Mark IV (Thermo Fisher) at 15 °C and 100% humidity ...
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bioRxiv - Genomics 2022Quote: ... Each guide was then annealed with equal amounts of tracrRNA by heating at 95 °C for 5 min and then cooled down from 95 to 25 °C using a gradient of −1 °C/12 s in a Veriti 96-well gradient thermal cycler (ThermoFisher, CA) to obtain a concentration of 250 ng/μl gRNA-tracrRNA complex ...
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bioRxiv - Immunology 2022Quote: ... L981F amino acid changed compared to WA-1) or S variant genes (Wang et al., 2021a) using Lipofectamine 3000 transfection reagent (ThermoFisher, CA). Forty-eight hours after transfection ...
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bioRxiv - Genomics 2019Quote: ... followed by 40 cycles at 95°C for 15 s and 60°C for 1 min in a QuantStudio 12K Flex instrument (Applied Biosystems).
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bioRxiv - Microbiology 2019Quote: ... were cultured in Dulbecco’s modified Eagle medium/nutrient mixture F12 (PAN-Biotech, Aidenbach, Germany) supplemented with 10% FBS and 1% penicillin-streptomycin (Thermo Fisher) or in MEM supplemented with 10% FBS and 0.5% neomycin.
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bioRxiv - Physiology 2021Quote: ... The grids were blotted for 1-1.5 s with an ashless Whatman® Grade 540 filter paper in a Vitrobot Mark IV (ThermoFisher Scientific) and rapidly plunged into liquid ethane ...
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bioRxiv - Cell Biology 2021Quote: ... HEMs were cultured in Medium 254 supplemented with 1% human melanocyte growth supplement (S-016-5, Cascade Biologics/Thermo Fisher Scientific) and 100 units/mL penicillin/streptomycin ...
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bioRxiv - Neuroscience 2022Quote: ... and cells were treated for 20-60 s with Proteinase K (1 μg/ml) in TE buffer (Invitrogen – Thermo Fisher Scientific). Single nNOS (+ ...
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bioRxiv - Neuroscience 2022Quote: ... and cells were treated for 20-60 s with Proteinase K (1 μg/ml) in TE buffer (Invitrogen – Thermo Fisher Scientific). Single nNOS (+ ...
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bioRxiv - Biochemistry 2022Quote: ... which were subsequently vitrified at 4°C and 100% humidity for 4.5 s with a blotting force of -1 on a Vitrobot IV (Thermo Fisher Scientific) with standard Vitrobot filter paper (ø55/20 mm ...
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bioRxiv - Molecular Biology 2021Quote: RNA was isolated from day 1 adult worms following three rounds of freeze-thaw in liquid nitrogen using Invitrogen’s Trizol extraction method and 1 μg of RNA was reverse transcribed to cDNA using SuperScript™ II Reverse Transcriptase (Invitrogen). Gene expression levels were measured using 1 μg of cDNA and SYBR™ Green PCR Mastermix (Applied Biosystems ...
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bioRxiv - Systems Biology 2022Quote: ... and the pellet washed with 1 ml cold acetone and sonicated for 5 s at 15% power using a Sonic Dismembrator Model 500 sonicator (Fisher Scientific), fitted with a microtip ...
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bioRxiv - Genetics 2022Quote: HEK293T cells and ARPE-19 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% FBS (BI, Cat.04-001-1ACS) and 1% Penicillin / Streptomycin (Gibco, Cat 15140122) at 37 °C with 5% CO2.
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bioRxiv - Immunology 2019Quote: ... and 45 cycles at 95 °C for 15 s and 60 °C for 1 min for hybridization and extension with the use of ABI 7500 equipment (Applied Biosystems). A cut-off value of Ct (Cycle Threshold ...
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bioRxiv - Microbiology 2019Quote: ... were grown in TYI-S-33 medium (Keister, 1983) supplemented with 10% adult bovine serum with 1% Penicillin/Streptomycin 1000 U (Gibco ™) and 0.05% bovine bile (ThermoFisher ™ ...
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bioRxiv - Molecular Biology 2020Quote: ... which were then blotted for approximately 8 s and immediately plunged in 1:2 ethane:propane (Carbagas) at liquid nitrogen temperature using a Vitrobot (Thermo Fisher Scientific). The Vitrobot chamber was kept at 4 °C and 100% humidity during the whole procedure.
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bioRxiv - Immunology 2020Quote: ... All media for endothelial and epithelial cells were supplemented with their appropriate BulletKits, 10% FBS (Biosera, UK) and 1% P/S (Gibco, UK). Treatment protocols (except for those using MucilAir™ cells ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10 % foetal bovine serum (v/v; Seralab, London, UK) and 1 % penicillin/streptomycin (p/s; Gibco, Thermo Fischer, UK). BSC-1 (African green monkey cell line ...
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bioRxiv - Microbiology 2020Quote: ... 40 cycles of 15 s at 95 ° C and 1 min at 60 ° C in Step-One-Plus Real-Time PCR System (Applied Biosystems). The standard curve was generated by diluting the DNA of the bacterium Escherichia coli ATCC 25922 in series of 102 – 10−8 (20 – 2 × 109 ng of DNA) ...
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bioRxiv - Microbiology 2020Quote: ... 40 cycles of 15 s at 95 °C and 1 min at 60 °C in Step-One-Plus Real-Time PCR System (Applied Biosystems). The proportion of bacteria in the microbiome was calculated based on the Ct (cycle threshold ...
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bioRxiv - Bioengineering 2021Quote: ... supplemented with fetal bovine serum (FBS, 10%) (Hyclone, South Logan, UT, USA), penicillin-streptomycin (P/S, 1%) (Gibco, Waltham, MA, USA) and SD208 (3μM ...
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bioRxiv - Neuroscience 2020Quote: ... Biocytin-filled neurons were labeled with a streptavidin marker conjugated to Alexa Fluor (AF) 488 (Invitrogen, S-32354; 1:500 dilution). Additionally ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 10 μl of stock alizarin red solution (1 g Alizarin Red S from Acros Organics, 400460100 in 100 ml 0.5% KOH) were added per 1 ml of 1% KOH ...