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Citations for Thermo Fisher :
2251 - 2300 of 10000+ citations for Glutathione S Transferase GST Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The MS/MS spectra were searched against GST-AIMP1 sequence database using Proteome Discoverer (version 1.3, Thermo Fisher Scientific) with SEQUEST search engine ...
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bioRxiv - Immunology 2022Quote: ... ELISA plates (96-well plates, Immunol4HBX, Thermo Fisher) were coated with a 50/50 mixture of His-Spy-RBD and RBD-Spy-His (2 μg/mL ...
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bioRxiv - Microbiology 2019Quote: ... SAB agar plates (5-cm plates, Fisher Scientific) inoculated with 200 μL of a M ...
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bioRxiv - Bioengineering 2020Quote: 96-well plates (Nunc MediSorp plates; Thermo Scientific) were coated overnight at 4 °C with 50 ng/well of purified antigen (recombinant RSVF or designed immunogens ...
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bioRxiv - Bioengineering 2020Quote: 96-well plates (Nunc MediSorp plates; Thermo Scientific) were coated overnight at 4 °C with 50 ng/well of purified antigen (recombinant RSVF or designed immunogens ...
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bioRxiv - Immunology 2020Quote: ... Maxisorb 96-well plates (Nunc-immuno plates, Dutscher) were coated overnight at room temperature (RT ...
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bioRxiv - Immunology 2022Quote: ... Nunc Immuno Plate MaxiSorp plates (Thermo Fisher Scientific) were coated either with 50 μg/ml NP4-BSA or 50 μg/ml NP26-BSA and incubated overnight at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... Custom PCR plates (TaqMan 96 well-plate, ThermoFisher) were used to assess mRNA expression on a QuantStudio 12K Flex Real-Time PCR System (ThermoFisher ...
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bioRxiv - Biophysics 2020Quote: ... mouse cell line was obtained from ATCC and cultured in Dulbecco’s modified Eagle medium (DMEM) (Wisent) supplemented with 10% fetal bovine serum (FBS) (Wisent) and 1% Penicillin-Streptomycin antibiotic (P/S) (Thermo Fisher). The cells were incubated at 37 °C in 5% CO2 environment and ...
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bioRxiv - Biochemistry 2021Quote: ... blotted for 1–2 s and plunge frozen into a liquid ethane/propane mixture with a Vitrobot Mark IV (Thermo Fisher) at 15 °C and 100% humidity ...
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bioRxiv - Genomics 2022Quote: ... Each guide was then annealed with equal amounts of tracrRNA by heating at 95 °C for 5 min and then cooled down from 95 to 25 °C using a gradient of −1 °C/12 s in a Veriti 96-well gradient thermal cycler (ThermoFisher, CA) to obtain a concentration of 250 ng/μl gRNA-tracrRNA complex ...
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bioRxiv - Immunology 2022Quote: ... L981F amino acid changed compared to WA-1) or S variant genes (Wang et al., 2021a) using Lipofectamine 3000 transfection reagent (ThermoFisher, CA). Forty-eight hours after transfection ...
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bioRxiv - Genomics 2019Quote: ... followed by 40 cycles at 95°C for 15 s and 60°C for 1 min in a QuantStudio 12K Flex instrument (Applied Biosystems).
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bioRxiv - Microbiology 2019Quote: ... were cultured in Dulbecco’s modified Eagle medium/nutrient mixture F12 (PAN-Biotech, Aidenbach, Germany) supplemented with 10% FBS and 1% penicillin-streptomycin (Thermo Fisher) or in MEM supplemented with 10% FBS and 0.5% neomycin.
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bioRxiv - Physiology 2021Quote: ... The grids were blotted for 1-1.5 s with an ashless Whatman® Grade 540 filter paper in a Vitrobot Mark IV (ThermoFisher Scientific) and rapidly plunged into liquid ethane ...
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bioRxiv - Cell Biology 2021Quote: ... HEMs were cultured in Medium 254 supplemented with 1% human melanocyte growth supplement (S-016-5, Cascade Biologics/Thermo Fisher Scientific) and 100 units/mL penicillin/streptomycin ...
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bioRxiv - Neuroscience 2022Quote: ... and cells were treated for 20-60 s with Proteinase K (1 μg/ml) in TE buffer (Invitrogen – Thermo Fisher Scientific). Single nNOS (+ ...
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bioRxiv - Neuroscience 2022Quote: ... and cells were treated for 20-60 s with Proteinase K (1 μg/ml) in TE buffer (Invitrogen – Thermo Fisher Scientific). Single nNOS (+ ...
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bioRxiv - Biochemistry 2022Quote: ... which were subsequently vitrified at 4°C and 100% humidity for 4.5 s with a blotting force of -1 on a Vitrobot IV (Thermo Fisher Scientific) with standard Vitrobot filter paper (ø55/20 mm ...
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bioRxiv - Molecular Biology 2021Quote: RNA was isolated from day 1 adult worms following three rounds of freeze-thaw in liquid nitrogen using Invitrogen’s Trizol extraction method and 1 μg of RNA was reverse transcribed to cDNA using SuperScript™ II Reverse Transcriptase (Invitrogen). Gene expression levels were measured using 1 μg of cDNA and SYBR™ Green PCR Mastermix (Applied Biosystems ...
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bioRxiv - Systems Biology 2022Quote: ... and the pellet washed with 1 ml cold acetone and sonicated for 5 s at 15% power using a Sonic Dismembrator Model 500 sonicator (Fisher Scientific), fitted with a microtip ...
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bioRxiv - Genetics 2022Quote: HEK293T cells and ARPE-19 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% FBS (BI, Cat.04-001-1ACS) and 1% Penicillin / Streptomycin (Gibco, Cat 15140122) at 37 °C with 5% CO2.
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bioRxiv - Immunology 2019Quote: ... and 45 cycles at 95 °C for 15 s and 60 °C for 1 min for hybridization and extension with the use of ABI 7500 equipment (Applied Biosystems). A cut-off value of Ct (Cycle Threshold ...
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bioRxiv - Microbiology 2019Quote: ... were grown in TYI-S-33 medium (Keister, 1983) supplemented with 10% adult bovine serum with 1% Penicillin/Streptomycin 1000 U (Gibco ™) and 0.05% bovine bile (ThermoFisher ™ ...
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bioRxiv - Molecular Biology 2020Quote: ... which were then blotted for approximately 8 s and immediately plunged in 1:2 ethane:propane (Carbagas) at liquid nitrogen temperature using a Vitrobot (Thermo Fisher Scientific). The Vitrobot chamber was kept at 4 °C and 100% humidity during the whole procedure.
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bioRxiv - Immunology 2020Quote: ... All media for endothelial and epithelial cells were supplemented with their appropriate BulletKits, 10% FBS (Biosera, UK) and 1% P/S (Gibco, UK). Treatment protocols (except for those using MucilAir™ cells ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10 % foetal bovine serum (v/v; Seralab, London, UK) and 1 % penicillin/streptomycin (p/s; Gibco, Thermo Fischer, UK). BSC-1 (African green monkey cell line ...
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bioRxiv - Microbiology 2020Quote: ... 40 cycles of 15 s at 95 ° C and 1 min at 60 ° C in Step-One-Plus Real-Time PCR System (Applied Biosystems). The standard curve was generated by diluting the DNA of the bacterium Escherichia coli ATCC 25922 in series of 102 – 10−8 (20 – 2 × 109 ng of DNA) ...
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bioRxiv - Microbiology 2020Quote: ... 40 cycles of 15 s at 95 °C and 1 min at 60 °C in Step-One-Plus Real-Time PCR System (Applied Biosystems). The proportion of bacteria in the microbiome was calculated based on the Ct (cycle threshold ...
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bioRxiv - Bioengineering 2021Quote: ... supplemented with fetal bovine serum (FBS, 10%) (Hyclone, South Logan, UT, USA), penicillin-streptomycin (P/S, 1%) (Gibco, Waltham, MA, USA) and SD208 (3μM ...
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bioRxiv - Neuroscience 2020Quote: ... Biocytin-filled neurons were labeled with a streptavidin marker conjugated to Alexa Fluor (AF) 488 (Invitrogen, S-32354; 1:500 dilution). Additionally ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 10 μl of stock alizarin red solution (1 g Alizarin Red S from Acros Organics, 400460100 in 100 ml 0.5% KOH) were added per 1 ml of 1% KOH ...
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bioRxiv - Immunology 2020Quote: ... SARS-COV-2 Spike-specific F(ab’)2 was isolated by affinity chromatography using recombinant antigen (1 mg SARS-CoV-2 S-2P or RBD) coupled to 0.05 mg dry NHS-activated agarose resin (Thermo Fisher Scientific) as follows ...
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bioRxiv - Pathology 2021Quote: ... His-tag recombinant SARS-CoV-2 Spike Protein S1/S2 (S-ECD) (1 ng/ml; ThermoFisher Scientific; aa11-1208; RP-87680) or Flag-tag recombinant SARS-CoV-2 Spike RBD (1 ng/ml ...
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bioRxiv - Microbiology 2020Quote: ... worms were resuspended in 200 μL of 0.1% Triton-X 100 in S Basal and vortexed for 1 min using zirconium beads (Thermofisher Scientific, NC0442292). The resulting lysate was serially diluted 5-fold and plated onto YPD agar plate to quantify the number of colony forming units (CFU ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were incubated for 20 min at 37°C in PBS containing 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-hexadecanoic acid (BODIPY FL C16; 1 μM; Thermo Fisher Scientific). To determine neutral lipid content ...
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bioRxiv - Molecular Biology 2020Quote: ... # 236-EG), 20 ng/ml recombinant human FGF basic protein (R&D, # 4114-TC),1% penicillin/streptomycin (P/S, Invitrogen, SV30010), 1% sodium pyruvate (Gibco ...
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bioRxiv - Immunology 2020Quote: ... was applied to the grids and blotted (Whatman #1 paper) for 2 to 8 s using Vitrobot Mark IV (Thermo Fisher) with a blot force of 5 ...
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bioRxiv - Bioengineering 2021Quote: ... density gradient and the isolated cells were plated at 1.8×10 cells/cm in DMEM-LG containing 10 % FBS and 1 % penicillin/streptomycin (P/S, Thermo Fisher Scientific) in an incubator at 37 °C and 5 % CO2 ...
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bioRxiv - Biophysics 2020Quote: ... All cell lines were cultured in Dulbecco’s modified Eagle medium (DMEM) (Wisent) supplemented with 10% fetal bovine serum (FBS) (Wisent) and 1% Penicillin-Streptomycin antibiotic (P/S) (Thermo Fisher). Cells were seeded on the patterns for 1hour at 37°C in 5% CO2 environment ...
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bioRxiv - Immunology 2022Quote: ... were transfected with Sanger sequencing-verified SARS-CoV-1 or variant-specific SARS-CoV-2 S expression plasmid with Lipofectamine LTX (Life Technologies) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by 40 cycles of 95°C for 15 s and 60°C for 1 min (Applied Biosystems QuantStudio 7 Flex). Primers used for qPCR reactions are listed in Supplementary Table S5 ...
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bioRxiv - Neuroscience 2022Quote: ... supplemented with 10% (v/v) fetal bovine serum (FBS; SAFC Biosciences) and 1% (v/v) Penicillin/Streptomycin (P/S; Gibco, Life Technologies). Primary fibroblasts were maintained in F-DMEM culture medium comprising DMEM ...
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bioRxiv - Neuroscience 2022Quote: ... Stimulations of individual neuromasts consisted in trials of 3 consecutive 1 s-long pressure-application of either control – aCSF alone with 10 µM Alexa 594 (Invitrogen, A10438) - or drug – aCSF with 10 µM Alexa 594 completed with 1 mM serotonin (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... 40 cycles of 15 s at 95°C, and 60°C for 1 min; ABI PRISM 7000 Sequence Detection System; Applied Biosystems). The relative abundance of parasite rRNA ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by 40 cycles of 15 s at 95⍰°C and 1 min at 60⍰°C using SYBER Green PCR Master Mix (Life Technologies) in the 7500HT system (Applied Biosystems) ...
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bioRxiv - Immunology 2022Quote: ... were transfected with Sanger sequencing-verified SARS-CoV-1 or variant-specific SARS-CoV-2 S expression plasmid with Lipofectamine LTX (Life Technologies) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... Frogs are euthanized by immersion for 10 min in 1 L water + 5 g Tricaine-S (Pentair via Fisher Scientific #NC0342409) + 10 g sodium bicarbonate (Fisher Scientific # MK-7396-500) ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by 40 cycles of 95°C for 15 s and 60°C for 1 min (Applied Biosystems QuantStudio 6 Flex). For testing of U7 snRNA level in U7 KD cells ...
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bioRxiv - Microbiology 2023Quote: ... cells were transfected with 1.5 ug of WT or mutant SARS-CoV-1 S plasmid using polyethylenimine (PEI) (Thermo Fisher Scientific). Cells were transfected for 24 hours ...