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Citations for Thermo Fisher :
2601 - 2650 of 10000+ citations for Glutathione S Transferase GST Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... 1h at RT and subsequently incubated overnight at 4°C in primary antibody solution using Rabbit anti-Green-Fluorescent-Protein (GFP; 1:1000 ThermoFisher Scientific, A6455). The following day sections were rinsed three times in PBTriton 0.5% and incubated overnight at 4°C in secondary antibody solution using Goat anti-Rabbit-Oregon Green 488 (1:500 Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were co-transfected at ∼80% confluence with wild-type (4 μg) or mutant SCN1A construct (4 μg) and enhanced green fluorescent protein (eGFP (1 μg) using Lipofectamine 3000 Reagent (Thermo Fisher Scientific) and incubated under the culture conditions above for 24 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed four times in PBS and incubated for 45 min with fluorescent-labeled secondary antibody in blocking solution (1:500, Alexa Fluor 568 Donkey anti-Rabbit IgG, (#A10042, Thermo Fisher Scientific)) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed three times in PBS and incubated for 0.5 h with fluorescent-labeled secondary antibody in blocking solution (1:500, Alexa Fluor 568 Goat anti-Rabbit IgG, (#A11036, Thermo Fisher Scientific)) ...
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bioRxiv - Immunology 2023Quote: ... Cells were then stained with fluorescent secondary antibodies (goat anti-rabbit Alexa Fluor 488, and/or goat anti-mouse Alexa Fluor 488; Invitrogen, 1:1,000), and finally washed with PBS three times for 5 min each ...
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bioRxiv - Cancer Biology 2023Quote: ... Red fluorescent ethidium homodimer (EthD-1) was added at the beginning of the tri-culture to detect dead cells (Invitrogen, Waltham, MA). For the quantification of dead/live cells ...
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bioRxiv - Molecular Biology 2023Quote: ... brain slices were washed 3X in 1X TBS and then incubated for 2 hours at RT with a fluorescent-tagged Alexa Fluor 568 anti-mouse secondary antibody (1:500; Life Technologies A11004). Brain sections were then washed 3X in 1X TBS ...
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bioRxiv - Neuroscience 2023Quote: ... They were then incubated at 4 °C overnight with primary antibody rabbit anti-Myosin VI (1:400 Proteus Bioscience) and mouse anti-green fluorescent protein (Invitrogen, cat. A11120). The tissues were incubated with 1:800 diluted secondary antibodies for 2 hrs at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... washed 3 times with PBT for 10 min at room temperature and incubated with the corresponding secondary fluorescent antibodies (1:100; Thermo Fisher Scientific) in blocking solution for 2 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: BHK-21 and DF-1 cells were plated at 1×105 cells/well in 24-well plates (Nunc). The following day ...
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bioRxiv - Neuroscience 2024Quote: ... and split 1:10-1:20 onto hESC-matrigel coated plates with Y-27632 (10 uM) (Thermo Fisher) in mTeSR1 media.
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bioRxiv - Neuroscience 2023Quote: ... plates in sensory neuronal induction media on day 0 (1:1 mix of Neurobasal (ThermoFisher cat. no. 21103049) and DMEM/F12 medium (ThermoFisher cat ...
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bioRxiv - Cancer Biology 2024Quote: ... Hydrogel-embedded PCLS were gently transferred to culture media in 24-well plates and maintained at 37°C with 5.0% CO2 in DMEM:F12 (1:1; Gibco) media with 0.1% fetal bovine serum (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell cycle analysis was performed by flow cytometry and in the final hour of drug treatment 10 μM EdU was added to the media containing drug to accurately identify cells in S-phase using a Click-iT™ EdU flow cytometry assay kit (Thermofisher, Waltham, MA, USA). Cells were harvested and fixed by 4% paraformaldehyde (15 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... the strips were washed 3 times in PBS-T before incubation in primary antibody (GST Tag Mouse anti-Tag, Clone: 8-326, Invitrogen) for 1 h at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... were PCR-amplified and cloned into the pDEST15 vector encoding an N-terminus GST tag using the Gateway Cloning Technology (Invitrogen)27 ...
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bioRxiv - Cell Biology 2021Quote: Human ProtoArray® Microarray (v5.0) containing 9483 unique human proteins expressed as GST-fusion in insect cells were purchased from Invitrogen. Arrays were blocked in 4°C cold blocking buffer (50 mM HEPES pH 7.4 ...
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bioRxiv - Cell Biology 2022Quote: His- and GST-tagged CFAP418 proteins were expressed in One Shot™ BL21 Star™ (DE3) cells (ThermoFisher, Waltham, MA) and purified using HisPur™ Ni-NTA resin and Pierce™ Glutathione Superflow Agarose ...
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bioRxiv - Molecular Biology 2020Quote: ... ORFs were cloned in pDEST15 (containing the GST tag) and pDEST17 Gateway (containing the His6 tag) vectors (Thermo Fisher Scientific). For Y2H assays ...
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bioRxiv - Biophysics 2024Quote: ... and then incubated with 50-100 μl 2.3 μM (0.1mg/ml) GST-WCA and various concentrations of Neutravidin (ThermoFisher, Cat. #31000) (8.3 μM (0.5mg/ml ...
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bioRxiv - Plant Biology 2024Quote: ... Recombinant protein with N-terminal 9x-His and GST tag was produced by amplifying the coding sequences of NRL5 and NRL5P335L and cloning into the expression vector pT7CFE1-NHIS-GST-CHA (Human In Vitro Protein Expression Vector, ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... fluorescently labeled GST-antibody (final concentration of 25 nM) and Nuclear Receptor Buffer F (A15668, A11131, PV4547, Thermo Fisher Scientific) supplemented with 1 μM compound or solvent only (DMSO ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were transfected with 2.5 μg of GST-tagged RIT1 plasmids or GFP control plasmid using Lipofectamine 3000 Reagent (ThermoFisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Transfections into NRK and COS-7 cells for fixed or live imaging experiments and for GST-RILP Rab7 activity pulldowns were performed using Lipofectamine 2000 (Invitrogen) per manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: GST-tagged full-length SYK and VenusFlag-tagged full-length FCER1G were generated using Gateway LR Clonase (Invitrogen, 11791-100) for mammalian cell expression ...
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bioRxiv - Plant Biology 2024Quote: ... The presence of prey and bait was detected using western blotting with monoclonal anti-GST and Anti-6xHis antibody (Invitrogen).
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bioRxiv - Plant Biology 2024Quote: ... and the proteins associated with the beads were subsequently resolved on SDS-PAGE before being detected by Western blot analysis with anti-GST (Invitrogen) and anti-atDjA1 antibodies.
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bioRxiv - Neuroscience 2024Quote: Mitoprobe JC-1 assay kit (Invitrogen) was used to monitor mitochondrial transmembrane potential using the Mito Probe™ JC-1 Assay Kit protocol ...
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bioRxiv - Microbiology 2019Quote: ... immunoassay plates (96-well plates; Nunc, Rochester, NY, USA) were coated with 100 ng/well of purified antigens at 4°C overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... The plate was applied to a plate magnet (Invitrogen) until the solution cleared and the supernatant was removed without disturbing the beads ...
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bioRxiv - Biochemistry 2019Quote: ... The ELISA plates (Nunc-Immuno Plate; Thermo Scientific, Denmark) were primarily coated with 1 μg TLR2 protein in 100 μl PBS buffer and incubated for 2 h at 37°C ...
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bioRxiv - Neuroscience 2019Quote: ... tissue culture plates (4-well plates; Nunc, Wiesbaden, Germany) were coated with poly-D-lysine (PDL ...
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bioRxiv - Physiology 2019Quote: ... The plate was applied to a plate magnet (Invitrogen) until the solution cleared and the supernatant was removed without disturbing the beads ...
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bioRxiv - Immunology 2022Quote: ... Immunlon 2HB plates 96-well plates (Thermo Scientific 3455) were coated with 50µl of Wuhan-Hu-1 RBD protein (gift of Institute for Protein Design ...
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bioRxiv - Microbiology 2022Quote: ... 96-well microtiter plates (Immulon 2HB plates, Thermo Scientific) were coated with sequentially diluted spRSV/A/Bernett or spRSV/B/BA in PBS (starting at 10 µg/mL or 15 µg/mL of total protein ...
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bioRxiv - Microbiology 2022Quote: ... The plate was applied to a plate magnet (Invitrogen) until the solution cleared and the supernatant was removed without disturbing the beads ...
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bioRxiv - Biochemistry 2022Quote: ... in 96-well white plates (Armadillo plate, Thermo Scientific) using an integration time of 120ms ...
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bioRxiv - Bioengineering 2021Quote: ... before seeding cells was split in two halves and stained independently using cell membrane fluorescent markers from the Vybrant™ Multicolor Cell-Labeling Kit (V22889, Thermo Fisher Scientific Inc., USA). One half was incubated with 5μM DiO Labeling Solution (Green) ...
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bioRxiv - Synthetic Biology 2020Quote: Ten percent of the final RNA amount was used for chemical labelling with Cy3 fluorescent dye using Arcturus Turbo Labeling Cy3 Kit (ThermoFisher, Applied Biosystems, Cat No. KIT0609). The chemical labelling procedure and subsequent removal of free Cy3 dye was performed following manufacturer’s protocol with 15 μg of RNA per reaction ...
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bioRxiv - Immunology 2022Quote: ... for at least 20 min and then stained with FluoroMyelin™ Green fluorescent myelin stain solution or BrainStain™ Imaging Kit (Molecular Probes, Invitrogen, Eugene, OR) diluted 1:300 in PBS for 20 min ...
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bioRxiv - Microbiology 2019Quote: ... and cultured in Minimum Essential Medium Alpha Modification (MEM-α, HyClone) with 20% fetal bovine serum (FBS, HyClone) and 1% penicillin-streptomycin (P/S, Gibco Life Technologies). Goat adipose-derived mesenchymal stem cells (gASCs ...
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bioRxiv - Neuroscience 2022Quote: ... it was gently pipetted into 10 ml of pre warmed complete RPMI medium (CM) consisting of basal RPMl-1640 supplemented with 10% heat-inactivated FBS and 1% penicillin-streptomycin (P/S; Thermo Fisher Scientific). Following centrifugation (300 g for 5 min at room temperature) ...
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bioRxiv - Molecular Biology 2020Quote: ... and the s-mEV pellet resuspended via tituration for 1 minute in 500μL Ultrapure Endotoxin-free 0.1M PBS (Thermo Fisher Scientific, MA, USA) and used immediately for quantification.
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bioRxiv - Biophysics 2022Quote: ... Grids were blotted for 4 s at 0 force and 1-s wait time before being vitrified in liquid propane using a Mark IV Vitrobot (Thermo Fisher Scientific). The blotting chamber was maintained at 4°C and 100% humidity during freezing.
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bioRxiv - Molecular Biology 2022Quote: ... 25 µM customized Nextera PCR primers 1 and 2 and 0.6% final concentration of 100X SYBR Green I (Invitrogen Cat #S-7563) in a thermocycler with the following parameters ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then stained with 20 µg/mL biotinylated S protein (S1/S2) or 1:1000 Anti-HS (Clone F58-10E4) (Fisher Scientific, NC1183789) in PBS containing 0.5% BSA ...
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bioRxiv - Cell Biology 2022Quote: ... in islet culture media (RPMI media with 11.1 mM D-glucose supplemented with 10% vol/vol fetal bovine serum (FBS) (Thermo: 12483020) and 1% vol/vol Penicillin-Streptomycin (P/S) (GIBCO: 15140-148)) at 37°C with 5% CO2 ...
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bioRxiv - Cancer Biology 2022Quote: ... resuspended at a concentration of 1×104 cells per mL in complete RPMI 1640 cell culture media (10% FBS, 1% P/S) with 50 μM β-mercaptoethanol (Gibco 21985023). 100 μL of this cell suspension was added to each well such that each tumoroid initially started with approximately 1×103 cells ...
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bioRxiv - Biochemistry 2022Quote: ... Then Grids were immediately blotted for 3.0 s with blot force of -1 and plunge-frozen in liquid ethane cooled by liquid nitrogen using Vitrobot (Thermo Fisher Scientific) at 4 °C and with 100% humidity.
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bioRxiv - Systems Biology 2022Quote: A whole kidney from each mouse was collected and homogenized by polytron based homogenizer for 15 s three times in 1 ml of cold 100mM TEAB buffer with 1X HALT protease and phosphatase inhibitor cocktail (Thermo Scientific, # 1861280). Lysates were centrifuged at 700 x G for 5 mins at 4 C ...