Labshake search
Citations for Thermo Fisher :
2351 - 2400 of 10000+ citations for 7 Chloro 3 nitro 3 4 dihydro 1H quinolin 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... Each sample was loaded onto an Acclaim PepMap trap column (75 µm × 2 cm, 3 µm, C18, 100 Å, Thermo Scientific) and then separated on a nanoEase BEH C18 column (150 µm × 100 mm ...
-
bioRxiv - Neuroscience 2024Quote: ... either for 2-3 cm or ran completely and stained with colloidal coomassie dye G-250 (Gel Code Blue Stain Reagent, Thermo Scientific). The lane was cut into bands ...
-
bioRxiv - Molecular Biology 2024Quote: Protein concentrations from fractions 2 and 3 of fed human milk and digesta were determined using a Micro BCATM kit (Pierce, Thermo Fisher), following manufacturer instructions.
-
bioRxiv - Biochemistry 2023Quote: ... cells were passaged every 2–3 days by washing twice with phosphate-buffered saline (PBS) and dissociation with 0.25% Trypsin (Life Technologies, 25200056).
-
bioRxiv - Cancer Biology 2024Quote: ... Recipient cells were incubated with either PKH67 dye-labelled or fluorescent-labelled sEVs for 2-3 days in media supplemented with 5% exosome-depleted FBS (Gibco, USA). Cells were imaged with time-lapse microscopy or washed with PBS ...
-
bioRxiv - Cancer Biology 2024Quote: For acute versus chronic proliferation measurements total cell number for acute and chronic was counted at day 0 and every 2-3 days until day 10 using Countess automated cell counter (Thermo Fisher). For Incucyte® proliferation assay plastic flat bottom 96 well plates were coated with 5 μg/mL human fibronectin in PBS for 1h in 37°C ...
-
bioRxiv - Microbiology 2023Quote: ... Samples were injected and concentrated on a trap column (PepMap100 C18, 3 μm, 100 Å, 75 μm i.d.×2 cm, Thermo Scientific) equilibrated with 0.05% trifluoroacetic acid in water ...
-
bioRxiv - Molecular Biology 2023Quote: ... Peptides were injected onto an Acclaim PepMap100 C18 trap column (75 μm i.d., 2 cm long, 3 μm, 100 Å, Thermo Scientific) and further separated on an Acclaim PepMap100 C18 analytical column (75 μm i.d. ...
-
bioRxiv - Molecular Biology 2022Quote: ... C18, 3 μm, 100A), then separated using analytical column Acclaim PepMap RSLC (75um x25cm, nanoViper, C18, 2 μm, 100A) (Thermo Fisher). The flow rate was 300 nL/min ...
-
bioRxiv - Microbiology 2023Quote: ... and applied to plasma-cleaned CF-300 2/1 grids before being blotted for 3 seconds in a Vitrobot Mark IV (Thermo Fisher) and plunge frozen into liquid ethane ...
-
bioRxiv - Cell Biology 2023Quote: ... Beads were gently washed 3 times with 1 ml of KPBS using a DynaMag-2 magnet (Thermo Fisher Scientific cat# 12321D). Beads were resuspended in 1 ml of KPBS and transferred to a new tube ...
-
bioRxiv - Plant Biology 2023Quote: Qdot-labeled Cel7A was prepared by mixing 3 nM biotinylated Cel7A with 2 nM Qdot 655 (Thermo Scientific; catalog number: Q10123MP) in 50 mM sodium acetate ...
-
bioRxiv - Molecular Biology 2023Quote: ... Peptide mixtures were injected in 0.1% TFA into a C18 Acclaim PepMap100 trap-column (75 μm ID × 2 cm, 3 μm, 100Å, Thermo Fisher Scientific) for 3 minutes at 5 μL/min with 2% ACN ...
-
bioRxiv - Neuroscience 2023Quote: ... the cell pellet was then resuspended in enzyme assay buffer again and sonicated at 3-second intervals for 2 minutes at 50% amplitude (Cat# FB120220, Fisher Scientific). The cell lysate was centrifuged at 1000 x g at 4°C for 10 minutes ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were typically passaged every 2-3 days by treating the cultures with 0.05% Trypsin-EDTA (Thermo Fisher Scientific, cat# 15400054) for 8-10 min at 37°C ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Peptides were loaded first on a Trap column (Acclaim PepMap 100 3 µm C18 column of 2 cm length and 75 µm inner diameter, ThermoFisher Scientific) and then injected on an analytical column (EasySpray 15 cm column 2 µm 100 Å C18 column with 75 µm inner diameter ...
-
bioRxiv - Biochemistry 2023Quote: ... High-resolution micrographs for single-particle reconstructions (Figure 2 and 3) were collected on a Titan Krios G4 (Thermo Fisher Scientific), which was operated at 300 kV acceleration voltage ...
-
bioRxiv - Cell Biology 2022Quote: ... HCEnCs were rinsed in DPBS and passaged at a ratio of 1:2 or 1:3 with TrypLE (Thermo Fisher Scientific) for 10-15 min at 37°C in 5% CO2 ...
-
bioRxiv - Cancer Biology 2023Quote: ... The tumors were placed into a chilled conical tube containing 2-3 mL of TrypLE Express Enzyme (Gibco; cat. no. 1260413) and transported on ice to a biosafety hood ...
-
Pan-tissue mitochondrial phenotyping reveals lower OXPHOS expression and function across tumor typesbioRxiv - Biochemistry 2023Quote: ... of 3 to 30% acetonitrile using a 2□μm EASY-Spray PepMap RSLC C18 75□μm × 250□mm column (Thermo Fisher) with a column temperature of 55□°C ...
-
bioRxiv - Microbiology 2023Quote: ... The products were enzymatically cleaned (3 ul of PCR reaction, 2 ul water, 1 ul of ExoSap-IT (Applied Biosystems 78200)) for 15 minutes at 37°C followed by 15 minutes at 80°C ...
-
bioRxiv - Cell Biology 2023Quote: ... slides were rehydrated with 25mM HEPES (Sytox Green) or dPBS (Hoechst, TOTO-3) for 2 minutes followed by incubation in 1µM Sytox Green (Invitrogen, Waltham, MA) in 25mM HEPES ...
-
Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: ... were transfected with wild-type human NOPR or NOPLight (0.8 μg DNA) and β-arrestin-2-mCherry (1 μg DNA) using 3 μL Lipofectamine 2000 (Thermo Fisher). After 24 h ...
-
bioRxiv - Microbiology 2024Quote: ... 4 µl of peptide mixtures (1µg) were loaded onto an Acclaim PepMap precolumn (75 µm x 2 cm, 3 µm, 100 Å; Thermo Scientific) equilibrated in solvent A and separated at a constant flow rate of 250 nl/min on a PepMap RSLC C18 Easy-Spray column (75 µm x 50 cm ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were grown in cell culture dishes (Falcon) and passaged every 2-3 days via trypsinization with 0.0S% Trypsin-EDTA (2S300-0S4, Thermo Fisher Scientific) at 80-90% confluency ...
-
bioRxiv - Neuroscience 2024Quote: ... cochleae were plated in six-well plates (2-3 explants/well) containing 1 ml media (growth medium DMEM (12430-054,Gibco Life Technologies) combined with 1% FBS (16000-044 ...
-
bioRxiv - Biochemistry 2024Quote: ... which was equipped with a 75 μm x 2 cm trap column packed with C18 3 μm bulk resins (Acclaim PepMap 100, Thermo Scientific) and a 75 μm x 15 cm analytical column with C18 2 μm resins (Acclaim PepMap RSLC ...
-
bioRxiv - Neuroscience 2024Quote: ... in 0.05 M TBS and then incubated in the dark for 2 h at room temperature with secondary antibodies: donkey anti-mouse Alexa Fluor 488 (3 µl/ml; Life Technologies) and donkey anti-rabbit Alexa Fluor 594 (5 µl/ml ...
-
bioRxiv - Biochemistry 2024Quote: ... 0.5 mM ATP and 1% fraction V BSA) and loaded with 3 μM Mag-Fura-2 AM (Thermo Fisher Scientific, #M1292) at room temperature for 30 min ...
-
bioRxiv - Biochemistry 2024Quote: ... onto a nanotrap column (75 μm i.d. x 2 cm precolumn, packed with Acclaim PepMap100 C18, 3 μm, 100 Å; Thermo Fisher) using an EASY-nLC 1200 system (Thermo Fisher) ...
-
bioRxiv - Genetics 2024Quote: 100,000 HEK293 cells in a 6-well plate were transfected with Antisense oligonucleotide harbouring 2’-O Me phosphorothioate modifications at each nucleotide position (Integrated technologies) using 3 ul of Lipofectamine 2000 (11668027, ThermoFisher Scientific) in one ml OPTIMEM I Reduced Serum Medium with no phenol red (11058021 ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Libraries were aliquoted in 10 mL (OD600 ∼ 0.2) and re-grown for 2-3 h to extract plasmids using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) for sequencing and for subcloning into the expression strain ...
-
bioRxiv - Developmental Biology 2019Quote: ... One-third to one embryo equivalents were loaded at per lane on 4%-12% or 8% Bolt® Bis-Tris (Invitrogen). GFP Rabbit IgG Polyclonal Antibody (Molecular Probes ...
-
bioRxiv - Biochemistry 2021Quote: ... 6,8-Difluoro-4-Methylumbelliferyl Phosphate (DiFMUP, #D22065) and 6,8-difluoro-7-hydroxy-4-methylcoumarin (DiFMU, #D6566) were purchased from Life Technologies.
-
bioRxiv - Cell Biology 2022Quote: ... Blocking buffer was replaced after one hour with 7 µg/mL mouse anti-occludin antibody (cat# 33-1500, Invitrogen) in PBS with 5% goat serum ...
-
bioRxiv - Neuroscience 2022Quote: ... We incubated overnight with the anti-GFP primary antibody at 4 degrees (1:1000, A-6455 Invitrogen, 0.1M PBS 0.3% tx100, 3% NGS). After abundant PBS washes ...
-
bioRxiv - Cell Biology 2021Quote: ... Rev: 5’-TCATTGAGACACCATTTGTC-3’ were cloned into pCR™4-TOPO® TA vector using the TOPO-TA cloning kit (Thermo Fisher 450030) and sequence verified.
-
bioRxiv - Genomics 2022Quote: ... Plasmids were mixed in a 3:1 weight ratio of total plasmid DNA:PEI in 4 mL of either OptiMeM™ (Gibco cat. # 31985-062) or serum-free DMEM per flask and then shaken vigorously for 30 sec ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... We extracted total RNA from 3-4 biological replicates per sex and line with a PureLink RNA purification kit (Thermo Fisher Scientific, USA) and validated the samples with the Bioanalyzer RNA 6000 Nano kit (Agilent) ...
-
bioRxiv - Neuroscience 2022Quote: Mammary glands were harvested from lactating GCaMP6f;K5CreERT2 mice diced into 3- to 4-mm3 pieces and loaded with CellTracker Red (1.5 μM, ThermoFisher C34552, ThermoFisher, Waltham, MA) for at least 30 min in DMEM/F12 containing 10% FCS ...
-
bioRxiv - Microbiology 2023Quote: ... The N concentrations were determined by dry combustion of a 3-4 mg sample with a Flash EA1112 elemental analyzer (Thermo Scientific, Rodano, Italy).
-
bioRxiv - Cell Biology 2023Quote: ... and lentiviral vector expressing GFP-UMOD-WT or GFP-UMOD-H177- R185 del at a ratio of 3:1:4 using Lipofectamine 3000 Reagent (ThermoFisher Scientific, Waltham, MA) following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... and two solid-phase extraction columns (150 µm × 3 cm) packed with MAbPac particles (C8 resin, 4 µm diameter, 300-Å pore size; Thermo Fisher Scientific) were used ...
-
bioRxiv - Immunology 2023Quote: ... Cells were washed 3 times with 200 µL PBS for 4 min before secondary antibodies and 1 µg/mL Hoechst (Thermo Fisher Scientific #33342) were added in 35 µL per well and incubated for 1 hour at room temperature ...
-
bioRxiv - Biophysics 2024Quote: ... protein fractions were mixed with SDS-free loading buffer in a 3:1 ratio and loaded on a Novex™ 4–20% Tris-Glycine Gels using NativeMark™ Unstained Protein Standards (Invitrogen™) (range from 20 to 1236 kDa ...
-
bioRxiv - Cancer Biology 2024Quote: ... and the packaging plasmids psPAX2 and pMD2.G at a ratio 4:3:1 in the presence of Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA). The supernatants containing lentiviral particles were collected 48 hours after transfection ...
-
bioRxiv - Microbiology 2024Quote: ... MIC values were measured every 3 to 4 days in 96-well plates in plate readers (Thermo Scientific Multiskan FC with SkanIt software) according to previous description (48) ...
-
bioRxiv - Cell Biology 2021Quote: ... and/or CellTracker™ Blue CMAC (100 μM, 7-amino-4-chloromethylcoumarin, ThermoFisher Scientific). Stained cultures were re-normalized to equal cell numbers (0.5 OD600/mL) ...
-
bioRxiv - Genomics 2021Quote: ... After adding the RT mix (7 µl H2O, 4 µl 5x RT buffer [Invitrogen] ...
-
bioRxiv - Biochemistry 2023Quote: ... 5-7 μg BACMID DNA was incubated with 4 μL Fugene (ThermoFisher, Cat# 10362100) in 200 μL of ESF 921 media for 30 minutes at 230C ...