Labshake search
Citations for Thermo Fisher :
2201 - 2250 of 10000+ citations for 7 Chloro 3 nitro 3 4 dihydro 1H quinolin 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pharmacology and Toxicology 2019Quote: The inhibition in cell viability exerted by different treatments for one or three cycles of 48h was evaluated by using the soluble tetrazolium salt 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay (Life Technologies, Eugene, USA). In living cells ...
-
bioRxiv - Biochemistry 2020Quote: The gel was run for 2-3 cm and stained with colloidal coomassie dye G-250 (Gel Code Blue Stain Reagent, Thermo Scientific) after which each lane was cut out ...
-
bioRxiv - Cell Biology 2020Quote: ... Peptides were first loaded onto a nano trap column (Acclaim PepMap100 C18, 3 μm, 100Å, 75 μm i.d. × 2 cm, Thermo Fisher Scientific), and then separated on a reversed-phase EASY-Spray analytical column (PepMap RSLC C18 ...
-
bioRxiv - Plant Biology 2021Quote: ... qRT-PCR was performed with 2 × RealStar Green Power Mixture (gene star) in a QuantStudio 3 Real-Time PCR System (Applied Biosystems). Three biological replicates with three technical replicates were run for all genes ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Cells were subcultured at a 1:5 to 1:10 ratio every 2–3 d using Trypsin-EDTA (Gibco #25300-054). The HEK293FT cell line tested negative for mycoplasma with the MycoAlert Mycoplasma Detection Kit (Lonza #LT07-318).
-
bioRxiv - Plant Biology 2020Quote: ... 15N-labeled seedlings were then dried for 2-3 days at 65°C and further analyzed by Thermo Finnigan Delta plus XP IRMS (ThermoFisher Scientific). For primary root length determination ...
-
bioRxiv - Developmental Biology 2020Quote: ... Probe 2 (mutation; 5’ FAM – CTC CCG CCT GAT T 3’) and Platinum Quantitative PCR SuperMix-UDG w/ROX (Life Technologies). The products were amplified and analysed on an ABI StepOne PCR machine.
-
bioRxiv - Microbiology 2020Quote: ... Samples were injected and concentrated on a trap column (PepMap100 C18, 3 µm, 100 Å, 75 µm i.d. x 2 cm, Thermo Scientific) equilibrated with 0.05% TFA ...
-
bioRxiv - Physiology 2020Quote: ... Tissues were washed 2-3 times with PBS buffer to and were finally mounted with ProLong Diamond antifade mounting media (ThermoFisher Scientific) on a superfrost plus microscope slide (Fisherbrand ...
-
bioRxiv - Biochemistry 2021Quote: ... 2 μL of each sample were loaded onto a RP-C18 column (15 mm × 75 μm × 3 μm, Thermo Fisher Scientific). 2% acetonitrile with 0.1 % formic acid (A ...
-
bioRxiv - Immunology 2020Quote: ... D-PBS (D-PBS without Ca++ and Mg++) supplemented with 2% FBS and 3 mM cell culture grade EDTA (Life Technologies; Thermofisher) prior to monocyte isolation ...
-
bioRxiv - Immunology 2021Quote: ... Peptides were loaded onto an Acclaim PepMap 100 (75µm x 2 cm) C18 (3 µm, 100 Å) pre-column and separated on an EASY-Spray column (Thermo Scientific; ID 75µm x 50 cm ...
-
bioRxiv - Cancer Biology 2022Quote: ... cell density was adjusted to 106 cells/mL (and was never allowed to go over 3×106 cells/mL) and puromycin selection (2 μg/mL, Gibco, A1113803) was started ...
-
bioRxiv - Biochemistry 2022Quote: ... Between 2 to 3 million cells were used per electroporation and were resuspended in 90 μl of buffer R (Thermo Fisher). Proteins were spun down at 16700 g for 10 minutes and then diluted with buffer R in a 1:1 ratio ...
-
bioRxiv - Genetics 2022Quote: ... primary rat astrocytes were thawed into T75 flasks and fed once every 2-3 days with glial media {(MEM (Thermofisher 11095080) supplemented with 5% FBS (Gemini 900-108) ...
-
bioRxiv - Microbiology 2022Quote: Proteins (8 μM final) were incubated with 0.16 mM of (2'-(or-3')-O-(trinitrophenyl) adenosine triphosphate (TNP-ATP) (Thermo Fisher Scientific), in 50 μl of Binding Buffer (10 mM HEPES-KOH (pH 8) ...
-
bioRxiv - Microbiology 2022Quote: ... The grids were blotted with force 2 for 3 seconds and plunged into liquid ethane using a Vitrobot (FEI Thermo Fisher). Cryo-EM data were collected with a Titan Krios microscope (FEI ...
-
bioRxiv - Molecular Biology 2023Quote: ... Peptide mixtures were injected in 0.1% TFA on a C18 Acclaim PepMap100 trap-column (75 μm ID x 2 cm, 3 μm, 100Å, Thermo Fisher Scientific) for 3 min at 6 μL/min with 2% ACN ...
-
bioRxiv - Molecular Biology 2022Quote: ... The grids were washed by dipping 2 times in PBS (37°C) before 3 μL of 1 μm Dynabeads (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2022Quote: OE sections from mice injected with retrograde tracer were stained with Alexa Fluor 555- conjugated streptavidin to detect biotin-labeled dextran (1:500 in phosphate-buffered saline containing 3 % normal donkey serum and 0.3 % Triton-X100 for 2 h at room temperature, S32355, Thermo Fisher Scientific), or GAP43 (see above ...
-
bioRxiv - Microbiology 2023Quote: ... Samples were injected and concentrated on a trap column (PepMap100 C18, 3 μm, 100 Å, 75 μm i.d.×2 cm, Thermo Scientific) equilibrated with 0.05% trifluoroacetic acid in water ...
-
bioRxiv - Molecular Biology 2023Quote: ... Peptides were injected onto an Acclaim PepMap100 C18 trap column (75 μm i.d., 2 cm long, 3 μm, 100 Å, Thermo Scientific) and further separated on an Acclaim PepMap100 C18 analytical column (75 μm i.d. ...
-
bioRxiv - Molecular Biology 2022Quote: ... C18, 3 μm, 100A), then separated using analytical column Acclaim PepMap RSLC (75um x25cm, nanoViper, C18, 2 μm, 100A) (Thermo Fisher). The flow rate was 300 nL/min ...
-
bioRxiv - Microbiology 2023Quote: ... and applied to plasma-cleaned CF-300 2/1 grids before being blotted for 3 seconds in a Vitrobot Mark IV (Thermo Fisher) and plunge frozen into liquid ethane ...
-
bioRxiv - Cell Biology 2023Quote: ... Beads were gently washed 3 times with 1 ml of KPBS using a DynaMag-2 magnet (Thermo Fisher Scientific cat# 12321D). Beads were resuspended in 1 ml of KPBS and transferred to a new tube ...
-
bioRxiv - Plant Biology 2023Quote: Qdot-labeled Cel7A was prepared by mixing 3 nM biotinylated Cel7A with 2 nM Qdot 655 (Thermo Scientific; catalog number: Q10123MP) in 50 mM sodium acetate ...
-
bioRxiv - Molecular Biology 2023Quote: ... Peptide mixtures were injected in 0.1% TFA into a C18 Acclaim PepMap100 trap-column (75 μm ID × 2 cm, 3 μm, 100Å, Thermo Fisher Scientific) for 3 minutes at 5 μL/min with 2% ACN ...
-
Development of a genetically-encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2023Quote: ... were transfected with wild-type human NOPR or NOPLight (0.8 µg DNA) and β-arrestin- 2-mCherry (1 µg DNA) using 3 µL Lipofectamine 2000 (Thermo Fisher). After 24 h ...
-
bioRxiv - Neuroscience 2023Quote: ... the cell pellet was then resuspended in enzyme assay buffer again and sonicated at 3-second intervals for 2 minutes at 50% amplitude (Cat# FB120220, Fisher Scientific). The cell lysate was centrifuged at 1000 x g at 4°C for 10 minutes ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were typically passaged every 2-3 days by treating the cultures with 0.05% Trypsin-EDTA (Thermo Fisher Scientific, cat# 15400054) for 8-10 min at 37°C ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Peptides were loaded first on a Trap column (Acclaim PepMap 100 3 µm C18 column of 2 cm length and 75 µm inner diameter, ThermoFisher Scientific) and then injected on an analytical column (EasySpray 15 cm column 2 µm 100 Å C18 column with 75 µm inner diameter ...
-
bioRxiv - Biochemistry 2023Quote: ... High-resolution micrographs for single-particle reconstructions (Figure 2 and 3) were collected on a Titan Krios G4 (Thermo Fisher Scientific), which was operated at 300 kV acceleration voltage ...
-
bioRxiv - Cell Biology 2022Quote: ... HCEnCs were rinsed in DPBS and passaged at a ratio of 1:2 or 1:3 with TrypLE (Thermo Fisher Scientific) for 10-15 min at 37°C in 5% CO2 ...
-
bioRxiv - Cancer Biology 2023Quote: ... The tumors were placed into a chilled conical tube containing 2-3 mL of TrypLE Express Enzyme (Gibco; cat. no. 1260413) and transported on ice to a biosafety hood ...
-
Pan-tissue mitochondrial phenotyping reveals lower OXPHOS expression and function across tumor typesbioRxiv - Biochemistry 2023Quote: ... of 3 to 30% acetonitrile using a 2□μm EASY-Spray PepMap RSLC C18 75□μm × 250□mm column (Thermo Fisher) with a column temperature of 55□°C ...
-
bioRxiv - Microbiology 2023Quote: ... The products were enzymatically cleaned (3 ul of PCR reaction, 2 ul water, 1 ul of ExoSap-IT (Applied Biosystems 78200)) for 15 minutes at 37°C followed by 15 minutes at 80°C ...
-
bioRxiv - Cell Biology 2023Quote: ... slides were rehydrated with 25mM HEPES (Sytox Green) or dPBS (Hoechst, TOTO-3) for 2 minutes followed by incubation in 1µM Sytox Green (Invitrogen, Waltham, MA) in 25mM HEPES ...
-
bioRxiv - Biochemistry 2023Quote: ... cells were passaged every 2–3 days by washing twice with phosphate-buffered saline (PBS) and dissociation with 0.25% Trypsin (Life Technologies, 25200056).
-
bioRxiv - Neuroscience 2024Quote: ... Control 1 (C1) and 2 (C2) were derived by StemBANCC from an unaffected volunteer and control 3 (C3) was purchased from Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2024Quote: ... Fractionated samples and bead-enriched samples were individually loaded onto a C18 trap column (3 μm, 75μm × 2 cm, Thermo Fisher Scientific) connected in-line to a C18 analytical column (2 μm ...
-
bioRxiv - Immunology 2024Quote: ... We electroporated 3 μg of ODNs or gDNA into 2 × 106 HEK293 and 293A cells by Neon Transfection System (Thermo Fisher) based on the manufacturer’s introductions.
-
bioRxiv - Neuroscience 2024Quote: ... Each sample was loaded onto an Acclaim PepMap trap column (75 µm × 2 cm, 3 µm, C18, 100 Å, Thermo Scientific) and then separated on a nanoEase BEH C18 column (150 µm × 100 mm ...
-
bioRxiv - Cancer Biology 2024Quote: ... Recipient cells were incubated with either PKH67 dye-labelled or fluorescent-labelled sEVs for 2-3 days in media supplemented with 5% exosome-depleted FBS (Gibco, USA). Cells were imaged with time-lapse microscopy or washed with PBS ...
-
bioRxiv - Cancer Biology 2024Quote: For acute versus chronic proliferation measurements total cell number for acute and chronic was counted at day 0 and every 2-3 days until day 10 using Countess automated cell counter (Thermo Fisher). For Incucyte® proliferation assay plastic flat bottom 96 well plates were coated with 5 μg/mL human fibronectin in PBS for 1h in 37°C ...
-
bioRxiv - Molecular Biology 2024Quote: Protein concentrations from fractions 2 and 3 of fed human milk and digesta were determined using a Micro BCATM kit (Pierce, Thermo Fisher), following manufacturer instructions.
-
bioRxiv - Neuroscience 2024Quote: ... either for 2-3 cm or ran completely and stained with colloidal coomassie dye G-250 (Gel Code Blue Stain Reagent, Thermo Scientific). The lane was cut into bands ...
-
bioRxiv - Developmental Biology 2019Quote: ... One-third to one embryo equivalents were loaded at per lane on 4%-12% or 8% Bolt® Bis-Tris (Invitrogen). GFP Rabbit IgG Polyclonal Antibody (Molecular Probes ...
-
bioRxiv - Biochemistry 2021Quote: ... 6,8-Difluoro-4-Methylumbelliferyl Phosphate (DiFMUP, #D22065) and 6,8-difluoro-7-hydroxy-4-methylcoumarin (DiFMU, #D6566) were purchased from Life Technologies.
-
bioRxiv - Cell Biology 2022Quote: ... Blocking buffer was replaced after one hour with 7 µg/mL mouse anti-occludin antibody (cat# 33-1500, Invitrogen) in PBS with 5% goat serum ...
-
bioRxiv - Cell Biology 2021Quote: ... and/or CellTracker™ Blue CMAC (100 μM, 7-amino-4-chloromethylcoumarin, ThermoFisher Scientific). Stained cultures were re-normalized to equal cell numbers (0.5 OD600/mL) ...