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Citations for Thermo Fisher :
2351 - 2400 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: Neurons were transfected at DIV 1-3 using Lipofectamine 2000 (Invitrogen). For one Ø18-mm coverslip seeded with 100,000 neurons ...
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bioRxiv - Immunology 2021Quote: ... and goat anti-mouse Cyanine 3 (Life technologies, A10521, 1:1000). Images for BrdU staining were taken using a Zeiss Confocal (LSM710 META) ...
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bioRxiv - Microbiology 2020Quote: ... 3 μl of DNA ladder (1 Kb Plus DNA Ladder; Invitrogen), 6 μl of positive control and 10μl of template DNA were ran on 2.5% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μM FluoZin-3 tetrapotassium salt (Thermo Fisher Scientific, Waltham, MA) was then added to each reaction ...
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bioRxiv - Cell Biology 2022Quote: ... Incubation with TO-PRO-3 (1:100, ThermoFisher Scientific Ref. T3605) was performed along with secondary antibody incubation to stain nucleic acid ...
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bioRxiv - Immunology 2022Quote: ... 3 µl of Dynabeads MyOne Carboxylic Acid (1 μm; ThermoFisher Scientific) were added at a concentration of 0.5 mg/ml to each of the samples ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA was labeled with ToPro-3 (1:5000; Invitrogen, Cat #T3605) in 0.3% PBST for 30 min at RT ...
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bioRxiv - Immunology 2024Quote: ... were coated with 3 µg ml-1 of streptavidin (Thermo Fisher) diluted in carbonate-bicarbonate buffer (E107 ...
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bioRxiv - Genomics 2022Quote: ... at a 1:3 ratio in Opti-MEM medium (Gibco, 11524456), incubated for 15 minutes at room temperature and added dropwise to the cells ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-14-3-3γ 1:2000 (Thermo fisher PA5-29690) and mouse anti-GAPDH (CSB-MA000195 ...
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bioRxiv - Physiology 2023Quote: ... counterstained with 1:30,000 TO-PRO-3 Iodide (Life Technologies, T3605), and then mounted in SlowFade Diamond mounting medium (Life Technologies ...
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bioRxiv - Immunology 2023Quote: ... Cy-3 conjugated anti-rabbit secondary antibody (Invitrogen, 1:300 dilution) was added and incubated for 0.5 hr ...
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bioRxiv - Neuroscience 2024Quote: ... larvae were immersed in 3 µM FM 1-43FX (ThermoFisher, F25255) in E3 for 30 s ...
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bioRxiv - Bioengineering 2021Quote: ... The 5(6)-Carboxyfluorescein N-hydroxysuccinimide was purchased from Thermo Fisher Scientific (ON ...
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bioRxiv - Developmental Biology 2022Quote: ... 5 and 6 dpf with TRIzol reagent (Thermo Fisher Scientific, 15596018) and incubated at 37° C for 30 minutes with RQ1 RNase-Free DNase (Promega ...
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bioRxiv - Cell Biology 2023Quote: Tubulin was labelled with (5(6)-TAMRA Succinimidyl Ester (Invitrogen, C1171) for fluorescence microscopy assays according to published methods (Consolati et al ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5 ng/uL human IL-6 recombinant protein (ThermoFisher PHC0066).
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bioRxiv - Microbiology 2024Quote: ... 6-5 cells were maintained in Dulbecco’s modified Eagle’s medium (Gibco) supplemented with 10% FetalPlex (Gemini Bio-Products) ...
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bioRxiv - Genomics 2020Quote: ... The chromatin was then cleared by centrifugation (12,000 g for 10 minutes at 4°C) and 80 μL of protein G DynaBeads (ThermoFisher, washed 3 times in cold ChIP buffer right before use ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatants were isolated by centrifugation at 12,000 × g at 4°C for 30 min and then mixed with 30 μl of Protein G Dynabeads (Invitrogen) that had been preincubated with antibodies ...
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bioRxiv - Biochemistry 2022Quote: ... expression clone cDNA or control cDNA (1 µg) was mixed with packaging plasmids MD2G (1 µg) and PSPAX2 (1 ug) in 600 µL per plate OPTIMEM (Gibco) and Lipofectamine™ 2000 transfection reagent (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: ... Antibody-chromatin complexes were isolated using a 1:1 mixture of magnetic Protein A- and Protein G-dynabeads (ThermoFisher Scientific) and washed three times with a solution of 50 mM HEPES-KOH ...
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bioRxiv - Immunology 2020Quote: ... lung tissue samples were homogenized in an equal volume (1 g/mL) of cold 1X PBS containing 1% penicillin/streptomycin (GIBCO). Tissue homogenates were centrifuged at 3000 rpm for 15 min at 4°C ...
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The G2-phase enriched lncRNA SNHG26 is necessary for proper cell cycle progression and proliferationbioRxiv - Molecular Biology 2021Quote: ... Soluble chromatin supernatant equivalent to 6 μg per IP was pre-cleared using 20 μl of a 1:1 mix of protein A and protein G Dynabeads (Invitrogen). Overnight IP was performed using 1 μg of anti-H3K4me3 (Diagenode ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-GAPDH, 1:5000 (CAB932Hu22, USCN) and horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat anti-mouse, 1:5000, G-21040, Invitrogen; Goat anti-rabbit ...
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bioRxiv - Immunology 2024Quote: ... the phage:antibody complexes were immunoprecipitated using 40 μL of a 1:1 mixture of protein A and protein G magnetic Dynabeads (Invitrogen) (4°C for 4 hours ...
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bioRxiv - Biochemistry 2022Quote: ... Anti-mouse HRP-conjugated (G-21040, 1:10000) and anti-rabbit HRP-conjugated (65-6120, 1:10000) secondary antibodies from Invitrogen.
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bioRxiv - Molecular Biology 2023Quote: ... chromatin-antibodies complexes were recovered by incubation with 30 μl of Dynabeads® Protein A/G 1:1 mix (ThermoFisher) for 2 h at 4°C under rotation ...
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bioRxiv - Cell Biology 2023Quote: ... 1% of input chromatin was saved and 70 μg of chromatin pre-clearing was done by mixing with pre-blocked Sepharose beads (1:1 vol. mix of Protein A and protein G beads; Invitrogen) for 1h at 4°C ...
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bioRxiv - Genomics 2023Quote: ... Sonicated samples were pre-cleared to remove background signal through incubation with a 1:1 mix of Protein A/G Dynabeads (InVitrogen). Antibody was added at a concentration of 1 µg/µL per sample and immunoprecipitated at 4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Coding sequences were amplified using total RNA from 7 days post-fertilization (dpf) zebrafish using the SuperScript IV One-step RT-PCR kit (ThermoFisher) using the primers listed in Table S1 ...
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bioRxiv - Microbiology 2023Quote: ... Quantitative Real-Time PCR was performed using KAPA SYBR FAST One-Step kit (KAPA) on a QuantStudio 7 Flex (ThermoFisher) with the following cycling conditions ...
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bioRxiv - Bioengineering 2024Quote: ... They were then transferred to 48-well plates (VTC-48, AS ONE Corp., Osaka, Japan) and cultured for 7 days using OncoPro medium (A5701201, Gibco) supplemented with 1% P/S ...
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bioRxiv - Plant Biology 2024Quote: ... and one 7 mm surgical cotton ball was placed in each well of the 96 well plates (Thermo Fisher scientific). Black plates were used to minimize light reflection and penetration ...
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bioRxiv - Neuroscience 2022Quote: ... Tax ID 10090) protein database (2017-6-7) using Proteome Discoverer (PD) 2.2 (Version 2.2.0.388; Thermo Fisher Scientific). Label-free quantification was also performed with PD 2.2 using precursor ions quantifier nodes ...
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bioRxiv - Genomics 2021Quote: ... qPCR was performed on Viia 6/7 Real time PCR system using SYBR Green master mix (Life Technologies). The primers used are listed in Supplementary table 1 ...
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bioRxiv - Cell Biology 2021Quote: ... These experiments were carried out with the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems). Expression of circRNAs was calculated relative to the housekeeping genes 18S or ACTB ...
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bioRxiv - Plant Biology 2024Quote: ... Data was analysed with QuantStudio 6 and 7 Pro Real-Time PCR Systems Software (Thermo Fisher Scientific, America). For RT-qPCR ...
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bioRxiv - Cell Biology 2024Quote: ... Z-stack images were taking on day 6-7 after seeding using EVOS™ 7000 Imaging System (Invitrogen). and used for quantification of organoids.
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bioRxiv - Immunology 2023Quote: ... iGB cells were collected after 6-7 days on iGC culture and were stained with Fura-2 (Invitrogen) at 1μM / ml ...
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bioRxiv - Bioengineering 2023Quote: ... HUVECs were cultured for 6-7 days prior to detachment with 0.25% trypsin-EDTA (Life Technologies, 25200-056) for use in experiments ...
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bioRxiv - Bioengineering 2023Quote: ... HUVECs were cultured for 6-7 days prior to detachment with 0.25% trypsin-EDTA (Life Technologies, 25200-056) for use in experiments.
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bioRxiv - Immunology 2024Quote: ... B-7-6 was produced in our own laboratory and was directly conjugated to DyLight (Pierce/Thermo Scientific) fluorochromes ...
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bioRxiv - Cancer Biology 2022Quote: ... To count the apoptotic events cells were treated with a fluorescent dye detecting Casp3/7 activation (CellEvent™ Caspase-3/7 Green Detection Reagent, Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Cancer Biology 2021Quote: ... clone IMAGE ID 4977050 was obtained from Source Bioscience and PCR cloned using oligos (Tet2fwd: 5’-GGGGACAAGTTTGTACAAAAAAGCAGGCTTAatgccaaatggcagtacagt-3’ and Tet2rev: 5’-GGGGACCACTTTGTACAAGAAAGCTGGGTTtcatacaaatgtgttgtaag-3’) into pDonor221 (Invitrogen Gateway, ThermoFisher) and sequenced ...
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bioRxiv - Developmental Biology 2021Quote: ... and the reverse primer containing the Sp6 promoter (5’ ATTTAGGTGACACTATAGCCTCTTCAGTTCCTTCTTCCATC-3’).The aqp1a.1 probe was generated from whole extracted cDNA at 30hpf and amplified with the primers (5’-GTCATGAACGAGCTGAAGAGC-3’) and (5’-GGGTCACTTTGAGGACATCTC-3’),incorporated into the pCR-BluntII-TOPO vector (Invitrogen, 45-0245) (containing both the SP6 and T7 promoters) ...
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bioRxiv - Genomics 2020Quote: ... The 16S rRNA gene was amplified from the extracted genomic DNA using 16S universal primers 27F (5’-AGA GTTTGATCMTGGCTCAG-3’) and 1492R (5’-GGTTACCTTGTTACGACTTC-3’) and sequenced using an automated ABI3730XL capillary DNA sequencer (Applied Biosystems, USA) for taxonomic identification at Cosmo Genetech (Seoul ...
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bioRxiv - Microbiology 2020Quote: ... 229E-RP reverse primer (5’-CCAACACGGTTGTGACAGTGA-3’) and the TaqMan minor groove binder (MGB) probe 229E-TP (FAM-5’-TCCTGAGGTCAATGCA-3’-NFQ-MGB; Thermo Fisher Scientific), derived from the HCoV 229E membrane protein gene sequence as described previously (Vijgen et al. ...
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bioRxiv - Microbiology 2021Quote: The mcr-1 gene was amplified from the clinical isolate ESBL20150072 (European Nucleotide Archive, accession number; SAMEA104060671) using primers: 5’-TTTTGGATCCCGGTTTTCGGGCTTTTTA-3’ and 5’-ATATGTCGACTCAGCGGATGAATGCGGT-3’ and Phusion polymerase (Thermo Fisher Scientific). PCR product was digested with BamHI and SalI and ligated into pACYC184 digested with the same enzymes (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... PCSK9 mRNA expression was assessed by real-time PCR using PCSK9 primer sets 5’-TGTCTTTGCCCAGAGCATC-3’ and 5’-GTCACTCTGTATGCTGGTGTC-3 (Integrated DNA Technologies) and SYBR Select Master Mix (ThermoFisher, ABI 4472908).