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Citations for Thermo Fisher :
2151 - 2200 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... To each sample 0.7 µL of reverse transcription mix (8:2:1:1 of Superscript IV 5X Buffer, 100 mM DTT, RNase Inhibitor, Superscript IV; ThermoFisher Scientific) was added ...
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bioRxiv - Microbiology 2021Quote: ... mixed at an 1:1 ratio in a test tube and added to wells of an 8-well slide (Thermo Fisher) coated with poly-lysine (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... then lysed in Triton lysis buffer (1% Triton, 150 mM NaCl, 50 mM Tris pH 8, 1× Halt protease-inhibitor cocktail (Thermo Scientific)) ...
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bioRxiv - Biophysics 2023Quote: ... and U-251 cells were seeded onto PLL-PEG-RGD-coated 8-well chambers in the respective medium with penicillin (1 unit/mL) and streptomycin (1 µg/mL; Gibco, Life Technologies) at densities between 0.5 x 104 to 2.5 x 104 cells/well ...
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bioRxiv - Cell Biology 2023Quote: ... coated #1.5 18 mm glass coverslips in DMEM (4.5 g/L-1 D-G-glucose) supplemented with 10% fetal bovine solution (Gibco™), 1% antibiotic solution (penicillin Streptomycin ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were incubated with fluorescently labelled secondary antibodies (1:1,000 G-anti-Rb-AlexaFluor-488, G-anti-M-AlexaFluor-555, ThermoFisher Scientific) and DAPI in PBS+ for 1.5 hr at room temperature before mounting with Aqua-Poly/Mount (Polysciences ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were anesthetized with urethane (3 g/kg body weight; Fisher Scientific) and transcardially perfused for one minute with gravity-fed ice-cold oxygenated (95% O2 ...
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bioRxiv - Microbiology 2022Quote: ... and rescued with GCP medium (15 g/L proteose peptone 3 (ThermoFisher), 1 g/L soluble starch (ThermoFisher) ...
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bioRxiv - Molecular Biology 2024Quote: ... the chromatin was incubated for 3 hours with Protein G Dynabeads (ThermoFisher; 10004D ...
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bioRxiv - Developmental Biology 2021Quote: ... The amplicon of the-2.3etv2 promoter was synthesised from zebrafish genomic DNA with a forward primer 5’- TATAGGGCGAATTGggtaccTTCAGTAAGCAGACTCCTTCAATCA -3’ and a reverse primer 5’- AGCTGGAGCTCCAccgcggTTCGGCATACTGCTGTTGGAC -3’ by Phusion High-Fidelity DNA Polymerase (Thermo Scientific) as an insert for In-Fusion Cloning (Takara Bio ...
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bioRxiv - Microbiology 2022Quote: ... The region of recombination (VP1 to 2C) was amplified using primers PV3-F (5′-GCAAACATCTTCCAACCCGTCC-3′) and PV1-R (5′-TTGCTCTTGAACTGTATGTAGTTG-3′) and Taq polymerase (Life Technologies) with an initial denaturing at 94°C for 3 min ...
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bioRxiv - Microbiology 2022Quote: ... 16S rRNA gene was amplified using primers 8F (5’-AGAGTTTGATCCTGGCTCAG-3’) and 1492R (5’-GGTTACCTTGTTACGACTT-3’) by colony PCR (Vaishnava et al., 2011) using DreamTaq Master Mix (ThermoFisher Scientific) and 0.2μM primers ...
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Targeted rescue of synaptic plasticity improves cognitive decline after severe systemic inflammationbioRxiv - Neuroscience 2021Quote: ... using the oligonucleotides WPRE-F 5’-TGCTTCCCGTATGGCTTTCAT-3’ and WPRE-R 5’-CAGCAAACACAGTGCACACC-3’ as primers and SYBR Select Master Mix (ThermoFisher Scientific). The measurements were performed with a CFX384 instrument (Biorad ...
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bioRxiv - Cancer Biology 2020Quote: ... using the following primers c=myb-F 5’-CCAAGTCAGGAAAACGCCACCTCG-3’ and c-myb-R 5’-GCTGTTGTTTAGCGGAGTTGGGCT-3’ and cloned into the dual promoter vector pCRII-TOPO (Life Technologies). The pCS2:runx1 probe was a gift from Leonard Zon ...
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bioRxiv - Genomics 2020Quote: ... using forward primer (5’-TGATTATCGACATCCCGTCA-3’) and reverse primer (5’-GTCTGGAATCTCATAGGTAG-3’) and run on an ABI 7500 thermocycler (Applied Biosystems). Primer specificity and capture temperature were determined by melt curve analysis ...
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bioRxiv - Neuroscience 2020Quote: Genomic DNA in the vicinity of the rs7143400 was amplified by PCR using the following primers 5’-GGTTGGGTGTGAATAGGAAT-3’ and 5’-TGCATGCCTGATTTATTTGG-3’ before digestion with Tsp45I enzyme (Thermo Scientific). Finally ...
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bioRxiv - Microbiology 2021Quote: ... Resulting colonies were screened for plasmid presence by PCR of a portion of the pESC-URA-lpt1 plasmid (Forward primer: 5’-TTGGAAACAGCTCCAAATCC-3’, Reverse primer: 5’ CCCAAAACCTTCTCAAGCAA-3’; ordered from ThermoFisher Oligos) and preserved as glycerol stocks.
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bioRxiv - Developmental Biology 2022Quote: ... were retrotranscribed from either 5’-CATGCTGCTGGTGGGTGTGCT-3’ or 5’-CCATAAAGCACCGGTGAGCAGAA-3’ endoglin specific reverse oligonucleotides (500 nM) using RevertAid H minus reverse Transcriptase (Thermo Scientific) 10 U/μl in 1X RevertAid H minus Buffer supplemented with 2 U/μl RNAse OUT (Thermo Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... using the ZIKV-F2 (5’-CAGCTGGCATCATGAAGAATC-3’) and ZIKV-R1 (5’-CACTTGTCCCATC TTCTTCTCC-3’) primers for African strain detection (ThermoFisher SCIENTIFIC) or the ZIKV-F1 (5’-CAGCTGGCATCATGAAGAACC-3’ ...
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bioRxiv - Molecular Biology 2022Quote: ... sgRNA sequences cutting near genomic loci of MLL3 Y4792 (5’-ACTATGGTCATCGAGTACAT-3’) and MLL4 Y5477 (5’-ACGATGGTCATCGAGTACAT-3’) were synthesized by Thermo Fisher’s custom in vitro transcription service ...
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bioRxiv - Cancer Biology 2019Quote: ... 97-mer oligonucleotides were purchased from Invitrogen and PCR amplified using the primers miRE-Xho-fw (5’ TGAACTCGAGAAGGTATATTGCTGTTGACAGTGAGCG-3’) and miRE-EcoOligo-rev (5’-TCTCGAATTCTAGCCCCTTGAAGTCCGAGGCAGTAGGC-3’) according to Phusion High-Fidelity kit (Thermo Scientific). The PCR product (139 bp ...
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bioRxiv - Plant Biology 2021Quote: The IKU2 promoter was amplified using the primers Prom-IKU2-B4 (5’-ggggacaactttgtatagaaaagttgGGTCTCTCTTGATAACGATTTG-3’) and Prom-IKU2-B1R (5’-ggggactgcttttttgtacaaacttgTGTTCTCTACGTCGGAAGG- 3’) and cloned into pDONR-P4-P1R (Life Technologies). A triple LR Gateway reaction (Life Technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... PCR was done on1 uL of cDNA using 10 µM of forward and reverse primers (Fw 5’-AAGATTCTCCTGAGCTGGGTC −3’ and Rv 5’-AGTCACTTTAGGTGGCCTTGG −3’, Life technologies) and 1 U Taq DNA polymerase (10342 ...
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bioRxiv - Immunology 2020Quote: ... or equimolar mix of two human APOBEC3A siRNAs (Silencer 45715 and 45810, respectively, with sense sequences 5’-GACCUACCUGUGCUACGAATT-3’ and 5’-GCAGUAUGCUCCCGAUCAATT-3’, Life Technologies) using Lipofectamine RNAiMAX (Life Technologies) ...
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bioRxiv - Neuroscience 2021Quote: ... or with a pool of two different USP30 siRNAs (D1: 5’-CAAAUUACCTGCCGCACAA-3’; D3, 5’-ACAGGAUGCUCACGAAUUA-3’, Dharmacon; siUSP30) by using Lipofectamine RNAiMAX (Thermo Scientific) following the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2021Quote: ... and human Oct 4 (For: 5’-CTTGCTGCAGAAGTGGGTGGAGGAA-3’/Rev: 5’-CTGCAGTGTGGGTTTCGGGCA-3’) PCRs were conducted using an ABI 7300 Real Time PCR System (Applied Biosystems). PCR cycling conditions were 95° C for 10 min. ...
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bioRxiv - Cancer Biology 2021Quote: ... The synthesized cDNA was amplified by EBNA 3C (1F and 1R) primers (5’-GAGAAGGGGAGC GTGTGTTGT-3’, 5’-GCTCGTTTTTGACGTCGGC-3’) by using regular PCR and adding Taq DNA polymerase (ThermoFisher, USA). The components of 25 μL reaction mixture contained 10 μL extracted DNA ...
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bioRxiv - Immunology 2021Quote: ... against the IAV nucleoprotein (forward: 5’-CAGCCTAATCAGACCAAATG-3’; reverse: 5’-TACCTGCTTCTCAGTTCAAG-3’) were assayed using SYBR Green Master Mix (Applied Biosystems) to confirm viral presence in the maternal lung.
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bioRxiv - Genetics 2020Quote: ... mouse cDNA was amplified with primers (F: 5-gtttatgggcctcaacctcatg-3, R: 5-caggcttcactccagctttttgg-3) and then enzyme digested with BsiEI (Thermofisher #FD0894). Genotyping result using this method is shown in Fig ...
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bioRxiv - Cell Biology 2024Quote: ... GAPDH-F (5’-GGA GCGAGATCCCTCCAAAAT-3’) and GAPDH-R (5’-GGCTGTTGTCATACTTCTCATGG-3’) using Power SYBR Green PCR Master Mix (Applied Biosystems). All reactions were carried out on the StepOnePlus Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2020Quote: Total RNAs from 7-d-old seedlings grown on 1/2 MS with or without 1 μM ABA were extracted using TRIzol (Invitrogen, Carlsbad, CA, USA) or Universal Plant Total RNA Extraction Kits (Spin-column)-I (BioTeke ...
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bioRxiv - Molecular Biology 2024Quote: ... overnight and then loaded with Fluo-4 (1:1000; Fluo-4 Calcium Imaging Kit; Invitrogen) and CellTracker Red (1:2000 ...
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bioRxiv - Plant Biology 2023Quote: ... CO2 mole fraction inside chamber was controlled by passing all airflow through the CO2 scrubber (indicating soda lime, 4-8 mesh size, CAS: 8006-28-8, Thermo Scientific) while carbon dioxide was supplied by an external cylinder (CAS 124-38-9 ...
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bioRxiv - Microbiology 2023Quote: ... niveus CO7 DNA ranging from 7 to 7 × 10−4 ng/µl was generated and quantified using a Nanodrop-8000 Spectrophotometer (Thermo Scientific, Wilmington DE, USA) (260/280 ratio of 1.89) ...
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bioRxiv - Microbiology 2020Quote: ... and resuspended in 0.1 ml of PBS supplemented with the lipophilic styryl membrane dye N-(3-triethylammoniumprpyl)-4-(p-diethylaminophenyl-hexatrienyl) pyridinium dibromide (FM4-64, Molecular Probes, Invitrogen; 10 μg.ml-1) (Pogliano et al. ...
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bioRxiv - Systems Biology 2023Quote: ... 1 vein graft slide containing 2-4 cross sections was double stained with the general macrophage marker Mac-3 (rat anti-mouse, Fisher Scientific, B550292, 1:600) and either iNOS (rabbit anti-mouse ...
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bioRxiv - Neuroscience 2024Quote: ... and diluted secondary antibodies were applied at 4 °C for approximately 3 hours using Alexa Fluor 488 goat anti-rabbit (1:1000; Invitrogen, Waltham, MA; Catalog #A11008); Alexa Fluor Plus 555 goat anti-rat (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: siRNA probes targeting sfrp2 in human mesenchymal cells and frizzled-5 and -6 in AEC2 cells were obtained from Thermo Fisher (Supplemental Table 4). In brief ...
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bioRxiv - Neuroscience 2021Quote: ... Larvae were anesthetized with diethyl-ether for 5-8 minutes (Acros Organics) before mounted in glycerol on top of a thin patch of agarose ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were maintained (37 °C, 5% CO2) in Essential 8 media (Gibco) on 23 µg/cm2 Matrigel (Corning ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 5 µg/mL FM 1-43 FX (Thermo Fisher Scientific F35355 ...
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bioRxiv - Cell Biology 2022Quote: ... Myofibers were treated with DRAQ-5 (1:1000; Invitrogen) to stain myonuclei and then mounted in Fluoromount G (SouthernBiotech).
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bioRxiv - Cancer Biology 2019Quote: ... 5 μL of Streptavidin C-1 beads (Thermo Fisher) were washed with Tween Wash Buffer (5 mM Tris-HCl pH 7.5 ...
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Caspase-8 Modulates Angiogenesis By Regulating A Cell Death Independent Pathway In Endothelial CellsbioRxiv - Developmental Biology 2019Quote: ... Claudin-5 (1:100, ref.34-1600, Thermo Scientific), cCasp-3 (1:100 ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 µL of Streptavidin C-1 beads (Thermo Fisher) were washed with Tween Wash Buffer (5 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-claudin 5 (1:200, ThermoFisher, 34-1600), rabbit anti-Sox2 (1:300 ...
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bioRxiv - Cancer Biology 2020Quote: ... incubated 5-10 minutes in DRAQ5 (1/1000, Thermofisher) for DNA staining and finally washed in PBS ...
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bioRxiv - Developmental Biology 2019Quote: ... mouse anti-Claudin 5 (Cldn5, 1:500, Invitrogen, 352588), rabbit anti-collagen IV (COL4 ...
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bioRxiv - Developmental Biology 2019Quote: ... and blocked with 5% BSA (BP1600-1, Fisher Scientific)/PBS ...
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bioRxiv - Cancer Biology 2019Quote: ... supplemented with 5% FBS and 1% sodium pyruvate (Invitrogen). A375 cells were grown in Dulbecco’s modified eagle medium with 4.5 g/l D-glucose ...