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Citations for Thermo Fisher :
2351 - 2400 of 10000+ citations for 6 TRIFLUOROMETHYL 1 2 3 4 TETRAHYDRO ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... and after immobilization of HER2- Nb and HER2-Nb-GEPII 1.0 was assessed using 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) reagent (ThermoFisher). After immobilization of HER2-Nb and HER2-Nb-GEPII 1.0 on the cell surface ...
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bioRxiv - Microbiology 2022Quote: ... All cells were passaged 2-3 times per week with 0.25% Trypsin/0.02% EDTA (Gibco Cat#25200056). Cells used in all experimental set-ups were between passage 5 and 30.
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bioRxiv - Biochemistry 2021Quote: ... 2′-(or-3′)-O-(N-Methylanthraniloyl) adenosine 5′-triphosphate (mant-ATP, Thermo-Fisher Scientific, Waltham, MA, USA) was serially diluted to concentrations of 5 -15 μM in assay buffer and added to the myosin at a final concentration of 1X -1.2X the final concentration of myosin ...
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bioRxiv - Cell Biology 2022Quote: ... All the LUVs contained 2 mol% of 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (DHPE)-tRed (Molecular Probes). Lipids were mixed in chloroform ...
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bioRxiv - Cancer Biology 2021Quote: ... antagomir 2 from Exiqon (miRCURY LNA microRNA Power Inhibitor; 4100464-002) and antagomir 3 from Thermo Scientific Dharmacon (miRIDIAN hairpin inhibitor ...
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bioRxiv - Biochemistry 2021Quote: ... Peptides were injected into a trap column (nanoViper C18, 3 μm, 75 μm × 2 cm, Thermo Scientific) with 12 μL of solvent A (0.1% formic acid ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid DNA (2-3 μg) was diluted in 400 μL Opti-MEM (11058021, Thermo Fisher Scientific, USA) spiked with 2-3 μL Plus reagent (15338100 ...
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bioRxiv - Genomics 2020Quote: ... the skin samples were washed 2-3 times with buffer saline (1X PBS; Gibco, Thermo Fisher Scientific). The adipose tissue and associated blood vessels were removed ...
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bioRxiv - Genomics 2020Quote: ... the skin samples were washed 2-3 times with buffer saline (1X PBS; Gibco, Thermo Fisher Scientific). The adipose tissue and associated blood vessels were removed ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were routinely passaged after 2-3 days or after reaching ∼80 % confluency using TrypLE Express (Gibco) for the detachment of cells from culture flasks ...
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bioRxiv - Immunology 2022Quote: ... 2 μm paraffin-embedded kidney sections were incubated with an anti-cleaved caspase-3 antibody (ThermoFisher Scientific). After incubation with goat anti-rabbit-HRP secondary antibody (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... in the reverse phase using a preconcentration column (75 μm DI × 2 cm, 3 μm, Thermo Scientific) and an analytical column (Acclaim PepMap C18 ...
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bioRxiv - Genetics 2022Quote: ... Cells were passaged at 70-80% confluence every 2-3 days using Trypsin 0.25% EDTA (Life Technologies). mESCs were plated at a density of 1 x 105 cells/ml ...
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bioRxiv - Microbiology 2022Quote: PBMCs were thawed according to standard procedure and rested 2-3 h in IMDM (Gibco/ThermoFisher Scientific) containing 5% human AB serum (SAB ...
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bioRxiv - Microbiology 2022Quote: PBMCs were thawed according to standard procedure and rested 2-3 h in IMDM (Gibco/ThermoFisher Scientific) containing 5% human AB serum (SAB ...
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bioRxiv - Molecular Biology 2023Quote: ... incubated for 2–3 minutes (mins) at room temperature in 0.05% (v/v) trypsin (Gibco 25300-054), and tapped to release ...
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bioRxiv - Molecular Biology 2023Quote: ... An Acclaim PepMap 100 trap column (75 μm × 2 cm, C18, 3 μm, 100 Å, Thermo Scientific) was used together with an analytical PepMap RSLC C18 column (150 μm × 15 cm ...
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bioRxiv - Cell Biology 2023Quote: ... after the first fast centrifugation step platelets were incubated with 3 µM Fura-2 AM (#F1221, Invitrogen) and 0.2% Pluronic F-127 (#P3000MP ...
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bioRxiv - Systems Biology 2023Quote: ... or F-12K medium (Caisson; Hela-S3 and PC-3) supplemented with 2 mM L-glutamine (Gibco), 100 U/mL penicillin ...
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bioRxiv - Biochemistry 2023Quote: ... cells were incubated with 1.2mM 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT, Thermo Fisher Scientific) solution ...
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bioRxiv - Systems Biology 2023Quote: Total RNAs (2–3 μg) were extracted from tobacco leaves using PureLink Plant RNA Reagent (Invitrogen, #12322012) and used for cDNA synthesis with M-MLV Reverse Transcriptase (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... The growth medium was changed every 2–3 days and consisted of DMEM/F12 (Fisher Scientific, 11330032) supplemented with 10% fetal bovine serum (Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... The supernatant was collected on day 3 and analyzed by human IL-2 ELISA Kit (Thermo Fisher) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2024Quote: Proliferation assays were performed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Thermo Fisher Scientific) assay ...
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bioRxiv - Cell Biology 2020Quote: ... BN-PAGE was performed using NativePAGE 3-12% or 4-16% Bis-Tris gels and associated running buffer (Invitrogen). The cathode buffer was supplemented with 0.002% (w/v ...
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bioRxiv - Immunology 2021Quote: ... Excess liquid was blotted for 3 seconds with a force of -4 using a Vitrobot Mark IV (Thermo Fisher) and plunge frozen into liquid ethane ...
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bioRxiv - Neuroscience 2020Quote: ... third instar larvae were dissected and stimulated with a HL-3 solution of 90 mM K+/0.5 mM Ca2+/4 μM fixable FM1-43 (Invitrogen) for 1 min ...
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bioRxiv - Genomics 2020Quote: ... Two TaqMan probes targeting the MLLT3 exon 3-4 and exon 9-10 splice junctions (ThermoFisher, Hs00971092_m1 and Hs00971099_m1) were used with cDNA diluted 1:5 in dH2O to perform qPCR for six replicates per sample on an Applied Biosystems Quant Studio 6 Flex ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were loaded with culture medium containing 3 μM of the fluorescent calcium indicator Fluo-4/AM (Life Technologies) and Pluronic F-127 (0.2% w/v ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibody incubation was performed for 3-4 days and DiD (Thermo Fisher Scientific-Molecular Probes L7781; 1µg/ml) labeling was added from the second day to show the whole brain morphology ...
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bioRxiv - Synthetic Biology 2021Quote: N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine triethylammonium salt (NBD-PE) was supplied by Thermo Fisher Scientific Inc ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were subcultured every 3-4 days as follows: cells were washed with phosphate buffered saline (PBS, Life Technologies) and then incubated with trypsin (Life Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... RT reactions were performed with 3-4 µg total RNA using oligo-dT and SuperScript III RT (ThermoFisher Scientific). QPCR was performed as previously described [20] ...
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bioRxiv - Cell Biology 2019Quote: ... Liposomes containing PtdIns(4)P- or PtdIns(3)P were independently labeled with DiI and DiD (Thermo Fisher Scientific) lipid dyes and detected by excitation at 561 nm and 640 nm ...
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bioRxiv - Bioengineering 2019Quote: ... C12 (4,4-Difluoro-5-Methyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid;D3823, Thermo Fisher Scientific), was added to the culture medium for a duration of 60 min and pumped through the media systems at 80 µl/h via positive pressure provided by a syringe pump ...
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bioRxiv - Molecular Biology 2020Quote: ... heated at 95°C for 3 min and seprated on a Novex WedgeWell 4-12% Tris-Glycine Gel (Invitrogen). Proteins were transferred to an PVDF Transfer Membrane (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... After 3 days the overlay was removed and cell monolayers were fixed with 4% formaldehyde (28906, Thermo Fisher Scientific) at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Uptake of fatty acids was quantified after incubation with 1uM 4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Hexadecanoic acid (Bodipy-FL C16, Thermo Fisher) at 37 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... Media was changed every 3 to 4 days and cells were passaged at nearly 90% confluence using 0.25% trypsin (Gibco). B16F0 cells were verified for mycoplasma contamination at Charles River Laboratories for cell line testing ...
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bioRxiv - Cell Biology 2021Quote: ... or 5 µM BODIPY™ FL C5-Ceramide (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)Sphingosine) (Invitrogen, D3521) together with 5 µM delipidated BSA (as a delivery carrier ...
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bioRxiv - Cell Biology 2021Quote: ... WT and SIRT1 KO mESCs cultured in serum-free ESGRO medium or serum-containing M10 medium were incubated with 5 µM BODIPY™ FL C5-Sphingomyelin (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)Sphingosyl Phosphocholine) (Invitrogen, D3522) or 5 µM BODIPY™ FL C5-Ceramide (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)Sphingosine ...
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bioRxiv - Plant Biology 2022Quote: ... pH 5.5 and 100 μM 3′,5′-dimethoxy-4′-hydroxyacetophenone (acetosyringone) (115540050; Acros Organics, dissolved in dimethyl sulfoxide (DMSO) (D8418 ...
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bioRxiv - Neuroscience 2022Quote: ... The craniotomy was then sealed with two glass coverslips (3 mm and 4 mm diameter, Thermo Fisher Scientific, UK) glued to one another using optical adhesive (Norland ...
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bioRxiv - Physiology 2022Quote: ... Cells were fixed with ice-cold 4% paraformaldehyde solution and cell nuclei were stained with To-Pro-3 (Invitrogen). Samples were mounted with Mowiol (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: BODIPY FL C16 (4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Hexadecanoic Acid, Invitrogen D3821) was used to assess the ability of 3T3s to transport fatty acids into the cell from their surroundings ...
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bioRxiv - Biochemistry 2023Quote: ... SDS-PAGE was carried out on either NuPAGE 4-12% Bis-Tris or 3-8% Tris-Acetate gels (Invitrogen). Western blotting analysis was carried out according to standard protocols with the antibodies listed in Table S5.
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bioRxiv - Cell Biology 2024Quote: ... cells were washed with PBS 3 times then fixed with 4% paraformaldehyde (PFA) in 1x PBS (Thermofisher, cat#043368.9M) at room temperature for 15 min in the dark ...
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bioRxiv - Biochemistry 2022Quote: ... Acly-/- MEFs were maintained at <80% confluence (passaged every 3-4 days) in high glucose DMEM (Gibco #11965-084) with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... cells at passage 3-4 were seeded in to black walled clear bottomed 96 well plates (Pierce, ThermoFisher Scientific) at a density of 7,500 cells per well ...
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bioRxiv - Biochemistry 2023Quote: ... BODIPY-CE (cholesteryl 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3- dodecanoate) was obtained from Life Technologies. Lipids (with the exception of NBD-LPC ...