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Citations for Thermo Fisher :
2401 - 2450 of 10000+ citations for 6 TRIFLUOROMETHYL 1 2 3 4 TETRAHYDRO ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... 3-4 days post-nucleofection (depending on cell confluency) selection was started using 400 ng/mL Puromycin (ThermoFisher A1113802) in FM (PFM) ...
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bioRxiv - Biochemistry 2023Quote: ... incubated for 30 min at 4 °C and analyzed on 3-12% gradient Bis-Tris native gels (Life Technologies) at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: BODIPY FL C16 (4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Hexadecanoic Acid, Invitrogen D3821) was used to assess the ability of 3T3s to transport fatty acids into the cell from their surroundings ...
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bioRxiv - Neuroscience 2024Quote: ... Mouse cochleae were fixed in 4% PFA for 3 hours and microdissected in cold 20% RNAlater (Thermo Fisher Scientific) in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... dried and cover slipped under glycerol:TBS (3:1) with Hoechst 33342 (1:1000; ThermoFisher Scientific). Slides were imaged at 10X magnification on a slide scanning microscope Olympus VS120 slide scanning microscope (Olympus ...
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bioRxiv - Microbiology 2023Quote: ... 3 μL of 1 mg mL−1 was vitrified using a Mark IV Vitrobot (Thermofisher). Prior to sample vitrification ...
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bioRxiv - Neuroscience 2020Quote: ... and pMD2G—at a 2:2:1 ratio using Lipofectamine 2000 (Thermo-Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... with 2 Units of S1 enzyme (ThermoFisher, 2 uL of a 1:100 dilution) and 1X S1 Buffer ...
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bioRxiv - Cell Biology 2020Quote: We seeded 2×105 cells / well into 6 well dishes and replaced media with 5% KSR (Knockout Serum Replacement) (Gibco) containing media the next day ...
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bioRxiv - Genetics 2021Quote: Live myofibers in the 6-well plate were stained with 2 µL of 5mg/mL of Hoechst (Molecular Probes, H1399) in 2 mL of 1X PBS for 5 minutes in a 37 °C with 5% CO2 incubator ...
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bioRxiv - Neuroscience 2019Quote: ... proliferating NSCs were plated on matrigel-coated 6- or 12-well plates and cultured in Neurobasal medium supplemented with B27 without vitamin A (2%, ThermoFisher), PenStrep (1% ...
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bioRxiv - Bioengineering 2020Quote: ... Primary fibroblast passages 2–6 were achieved in culture in 37°C in a 5% CO2 incubator with DMEM High Glucose (Gibco) with 10% FBS to 80% confluence in 100 mm Petri dishes ...
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bioRxiv - Neuroscience 2021Quote: ... samples were washed 5-6 times over 2 hours in PBT at room temperature and incubated with secondary antibodies (Invitrogen) diluted in NGS-PBT for 24 hours at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... for 6 min and seeding cells at a density of 2×106 cells/T75 flask (Nunc; Thermo Fisher Scientific, Singapore).
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bioRxiv - Microbiology 2021Quote: ... for 6 min and seeding cells at a density of 2×106 cells/T75 flask (Nunc; Thermo Fisher Scientific, Singapore).
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bioRxiv - Cell Biology 2021Quote: ... 2 µg DNA was mixed with 6 µL of Fugene HD in 100 µL of Opti-MEM (Thermo Fisher Scientific) and incubated at room temperature for 30 min ...
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bioRxiv - Biophysics 2022Quote: ... Purified Syb2 (29–96, S61C) was labeled with 6× molar excess BODIPY FL N-(2-aminoethyl)-maleimide (BDPY) (Molecular Probes) and SN25 FL (1–206 ...
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bioRxiv - Molecular Biology 2022Quote: ... Staining of nuclei with 4ʹ,6-diamidino-2-phenylindole (DAPI) was performed with mounting solution (ProLong Diamond Antifade Mountant with DAPI (ThermoFisher)) or 50% glycerol in 1×PBS.
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bioRxiv - Biochemistry 2021Quote: ... the cells were seeded on a 6-well plate with 2 ml of DMEM medium supplemented with 5% horse serum (Gibco). At 24 hours after transfection ...
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bioRxiv - Molecular Biology 2019Quote: ... Total RNA from HEK 293T cells (N = 2 biological replicates) or whole mouse brain (N = 6 biological replicates) was extracted using TRIzol (ThermoFisher) and treated with RNase-free DNase I (Promega) ...
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bioRxiv - Cancer Biology 2019Quote: The human myeloma cell line KJON (19) was cultured in RPMI with 5 % heat inactivated human serum (HS) and 2 ng/mL interleukin (IL)-6 (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... Transient transfections of wild-type or mutated MCM10-expressing plasmids were performed by transfecting 2×106 cells with 6 μg of DNA using Lipofectamine 2000 according to manufacturers’ instructions (Invitrogen).
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bioRxiv - Genomics 2020Quote: ... cells were harvested and grown for 5-6 passages in NPC expansion medium (selection medium with the addition of 2% B27 [GIBCO] ...
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bioRxiv - Systems Biology 2019Quote: ... for 30 minutes at 37C and plated with 2 million C57Bl/6 mouse embryonic fibroblasts as feeder cells (Gibco A34960) and with serum+LIF media composing of ...
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bioRxiv - Biophysics 2022Quote: ... Fractions containing S15 were consolidated and concentrated to ∼2 mg/mL using a 6 mL 3K molecular weight cutoff centrifugal concentrator (ThermoFisher), before being aliquoted and flash-frozen in liquid nitrogen for long-term storage at -80°C.
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bioRxiv - Neuroscience 2022Quote: ... CHO cells were plated at 1 x 106 cells/well in a 6-well plate and cultured for 24 hr in 2 ml of F12 medium (Gibco) supplemented with 10% FBS and 1% Glutamax (Gibco ...
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bioRxiv - Biochemistry 2023Quote: Doxycycline-inducible C2C12 cells were seeded for most treatments at 0.1X106 cells per well in 6-well plates with 2 ml of DMEM [high glucose DMEM with pyruvate (Gibco, 12800), 3.7 g/L NaHCO3 ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µg DNA was mixed with 6 µL of FuGENE HD in 100 µL of Opti-MEM (Thermo Fisher Scientific) and incubated at RT for 10 min before being added to HeLa cells grown in 35-mm glass-bottomed dishes (AGC Technology Solutions ...
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bioRxiv - Biophysics 2023Quote: ... Adherent semiconfluent HEK293SG cells cultured in DMEM:F12 medium supplemented with 10% FBS were transfected in 6-well plates with 2 μg/well of plasmid DNA complexed with Lipofectamine 2000 reagent (Invitrogen). The cells were incubated for 48 hrs at 37 °C under 7% CO2 ...
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bioRxiv - Plant Biology 2023Quote: ... Protein pellets were resuspended in urea buffer (6 M urea, 2 M thiourea, 100 mM ammonium bicarbonate, pH 8.0) and quantified using EZQ (Invitrogen/Life Technologies).
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bioRxiv - Cell Biology 2023Quote: The ROS dye assay was performed using Di(Acetoxymethyl Ester) (6-Carboxy-2’,7’-Dichlorodihydrofluorescein Diacetate) ROS dye (Invitrogen, D23844), a fluorogenic dye that is converted to 6-CarboxyFluorescein ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells at ∼50% confluency in 6-well plates were incubated with 2 mL Opti-MEM-I medium (Gibco, 31985062) supplied with 6 µL Lipofectamine 2000 transfection reagent (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... Cells were transfected in 6-well plates with 2 µg of the indicated constructs in the presence of Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... DNA was stained with 4′,6-diamidino-2-phenylindole (DAPI) in phosphate buffered saline (PBS) and cells were mounted with ProLong Gold Antifade reagent (Invitrogen).
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bioRxiv - Bioengineering 2024Quote: ... Hepatic endoderm specification was initiated on differentiation days 6 ─ 10 using RMPI supplemented with 2% B27 and insulin (ThermoFisher, #17504044), 10ng/ml FGF-2 ...
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bioRxiv - Neuroscience 2024Quote: ... with real time quantitative gene expression determined using using Taqman probles (Table 2) and analysed by the Quant Studio 6 Flex Real-Time PCR System (Invitrogen).
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bioRxiv - Molecular Biology 2020Quote: ... Cells were transfected with 4-6 μg linearized targeting vector DNA (ROSA26-SpCas9) using Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific, USA), selected with 1400-1600 μg/mL G418 (Genaxxon Bioscience ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA was isolated from liver tissues of SHR and WKY (4-6 weeks old) using the TRIzol reagent (Invitrogen, Waltham, USA) followed by purification using Nucleospin columns (Machery-Nagel ...
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bioRxiv - Cancer Biology 2021Quote: ... 6 × 105 of isolated MLEC were cultured in a 4-well chamber slide (Nunc Lab-Tek Chamber Slide System, Thermo Fisher Scientific) precoated with Extracellular Matrix Attachment Solution provided in the kit ...
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bioRxiv - Genetics 2020Quote: MEFs were fixed on 6-well glass bottom dishes with 4% PFA (diluted from 16% stock in PBS; ThermoFisher Scientific, Massachusetts, US), washed with PBS ...
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bioRxiv - Cell Biology 2020Quote: ... cells were typically transfected in a 6 well plate format with 20 μM siRNAs and 4 μL Lipofectamine RNAiMAX (Thermo Fisher Scientific); the depletion phenotype was inspected 72 h after transfection unless otherwise indicated in the text or the legends ...
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bioRxiv - Cancer Biology 2022Quote: ... B16F10-TR-shApoe cells were plated into a 6-well dish the day prior to transfection and transfected with 4 μg plasmid and TurboFect transfection reagent (Thermo Scientific, R0533) diluted in serum-free media ...
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bioRxiv - Immunology 2022Quote: ... Bacteria were harvested after 16–18 h at 3000 x g for 15 min at 4°C (Sorvall LYNX 6000 centrifuge, F9-6×1000 LEX rotor, Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... Nuclei was then isolated using a sucrose gradient and ultracentrifugation at 13,000 rcf for 45 mins at 4°C (Sorvall WX-100, F65L-6 x 13.5 rotor, Thermo Fisher, Waltham, MA USA). Isolated nuclei were resuspended in Nuclei PURE Prep kit storage buffer and RNAase inhibitor ...
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bioRxiv - Genetics 2021Quote: ... 6-VIC ® or 6-PET® (Life Technologies Corporation, Carlsbad, USA). During capillary electrophoresis ...
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bioRxiv - Cell Biology 2020Quote: ... in a 3:1 ratio with 1X penicillin/streptomycin (Gibco; 15070) and 5% FBS (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... rat Taste receptor type 1 member 3 (Tas1r3, Rn00590759_g1, Applied Biosystems) and rat Taste receptor ...
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The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: ... in Fibroblast medium (3:1 DMEM: Ham’s F12 Nutrient Mixture, Gibco) supplemented with 10% FCS ...
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bioRxiv - Cancer Biology 2019Quote: ... Sections were counterstained with ToPro-3 (1:1000 dilution; Life Technologies).
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bioRxiv - Cell Biology 2020Quote: ... and washed 3 times with 1x PBS (Fisher Scientific, BP399-1).