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Citations for Thermo Fisher :
2151 - 2200 of 10000+ citations for 6 TRIFLUOROMETHYL 1 2 3 4 TETRAHYDRO ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... 1.2 μl 1:4 (Sequenase:Sequenase dilution buffer (Thermo Fisher)) diluted Sequenase was added to reactions and incubated at 37°C for 8 minutes and 94°C for 8 minutes prior to transfer to ice.
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bioRxiv - Developmental Biology 2022Quote: ... diluted in 1 x PBS (Fisher Scientific, BP399-4). After 24 h of fixation ...
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bioRxiv - Bioengineering 2021Quote: ... Fluo-4 AM (10 μg ml−1, Life technologies) was added to the cell culture media for 30 min at 37°C.Scaffolds were then transferred in Tyrode’s buffer and videos were recorded either with no stimulation or while pacing at frequency of 1 and 1.5 Hz using c-PACE EM pace (IONOPTIX) ...
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bioRxiv - Bioengineering 2020Quote: ... 4% horse serum and 1% penicillin and streptomycin (Gibco). These cells consisted of an enriched population of beating cardiomyocytes ...
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bioRxiv - Microbiology 2022Quote: ... HRP (ThermoFisher; 1:5000, 60 min at 4°C). Detection was achieved with Pierce™ ECL Western Blotting Substrate using a ChemDoc XR+ (BioRad).
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bioRxiv - Developmental Biology 2019Quote: ... anti-Claudin 4 (Life Technologies, 36-4800, 1:200), anti-β5t (MBL ...
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bioRxiv - Neuroscience 2021Quote: ... and 1 µM Fluo-4 (F14201, Thermo Fisher Scientific) were pre-incubated for 30 minutes prior to image acquisition ...
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bioRxiv - Microbiology 2020Quote: ... 4 mM L-glutamine and 1% penicillin-streptomycin (Invitrogen) in 24-well plates for 7 days ...
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bioRxiv - Genomics 2020Quote: ... with 1 mg/mL Collagenase 4 (Fisher Scientific, #NC9836075), and 10-20 μg/mL DNAse I (StemCell Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... and anti-IL-4 (1:500; Thermo Fisher Scientific). Brain sections were then washed 4 × 5 min with PBS followed by overnight incubation at 4°C with the following secondary antibody dilutions in blocking solution ...
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bioRxiv - Cancer Biology 2022Quote: ... 4) Opal 620/anti-TdT (1:8, SEN28, Invitrogen), 5 ...
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bioRxiv - Microbiology 2023Quote: ... 1 cm into a 4-12% NuPage gel (Invitrogen) before staining with Coomassie blue (GelCode Blue Safe Protein Stain ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1 mL of ethyl acetate (E196-4, Fisher Scientific) was added to the culture medium and mixed for 1 hour at 28 °C ...
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bioRxiv - Bioengineering 2023Quote: ... and 4 μM Ethidium Homodimer-1 (Thermo Fisher Scientific) and imaged using a Zeiss Axio Observer ...
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bioRxiv - Microbiology 2023Quote: ... 1 cm into a 4-12% NuPage gel (Invitrogen) before staining with Coomassie blue (GelCode Blue Safe Protein Stain ...
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bioRxiv - Neuroscience 2023Quote: ... incubated overnight at 4° C (MAP2 1:1000 Invitrogen #MA5-12826 ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mL of ethyl acetate (E196-4, Fisher Scientific) was added to the culture medium and mixed for 1 hour at 28 °C ...
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bioRxiv - Immunology 2021Quote: ... EBM-2/EGM-2 medium was supplemented with puromycin (1 mg/ml, Gibco) for 3 d ...
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bioRxiv - Cell Biology 2022Quote: Transient genetic silencing of HOIP and FLOT1/2 was performed by reverse transfection of HT-29 cells with the following siRNAs: human siHOIP (RNF31) (Silencer® Select siRNA #1 s30108, #2 s30109, #3 s30110, 40 nM) (Thermo Fisher Scientific, Waltham, MA, USA), human FLOT1 (ON-TARGETplus Human FLOT1 siRNA SMART POOL ...
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bioRxiv - Neuroscience 2022Quote: ... Hippocampal neurons 13-15 days-in-vitro (DIV) were transfected with plasmid (2 h, 2-3 µg per dish) with Lipofectamine 2000 (Invitrogen, #52887, minimum 24 h). For pH imaging experiments ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 and 6 were directly cloned into pCR(tm)2.1-TOPO® using the TOPO® TA Cloning® Kit (Invitrogen) following manufacturer’s instructions ...
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bioRxiv - Zoology 2020Quote: ... Tissues were cut into small pieces with a scalpel and washed twice in ADPBS containing antibiotics prior to placing 4-6 tissue pieces into a 25 cm2 plug-seal tissue culture treated flask (BioLite, Thermo Scientific) containing 1.5 mL establishment medium to cover the tissue pieces ...
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bioRxiv - Cell Biology 2019Quote: ... 50 μg of enriched mitochondria were resolved approximately 6 cm into a pre-cast 4–12% Bis-Tris polyacrylamide gel (ThermoFisher Scientific). The lane was excised and cut in 8 approximately equal chunks and the proteins reduced ...
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bioRxiv - Biophysics 2021Quote: ... cells were transiently transfected with 4 μg mouse Piezo1-pIRES-EGFP in pcDNA3.1(+) or 4 μg YFP 40-48 hours before recording in 6-well plates using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... 4 µg of plasmid was transfected with 4 µL P3000 regent and 6 µL Lipofectamine 3000 as per scaled recommendations in the manufacturer’s protocol (Thermo Fisher Scientific). Medium containing lipid complexes was removed 4 h post transfection and replaced with exosome-depleted phenol red-free DMEM + 10% FBS + 2 mM L-glutamine (Thermo Fisher) ...
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bioRxiv - Bioengineering 2021Quote: ... 4 µg of plasmid was transfected with 4 µL P3000 regent and 6 µL Lipofectamine 3000 as per scaled recommendations in the manufacturer’s protocol (Thermo Fisher Scientific). Medium containing lipid complexes was removed 4 h post transfection and replaced with exosome-depleted phenol-free DMEM + 10% FBS + 2 mM L-glutamine ...
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bioRxiv - Plant Biology 2020Quote: ... Sections were cut at 4-6 μm and dried from a drop of dH2O onto Probe On Plus slides (ThermoFisher Scientific). Tissues sections were stained with 0.01% aqueous Toluidine Blue O ...
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bioRxiv - Cell Biology 2022Quote: ... the cerebral cortices of 4 to 6 P0 rats were dissociated and chopped in Ca++/Mg++-free Hank’s Balanced Salt Solution (HBSS; Invitrogen, Carlsbad, CA). The tissue pieces were dissociated in phosphate-buffered saline (PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... transferred to 1.6 mL Eppendorf tubes and spun at 2000 rcf for 5 minutes at 4°C in an angle rotor (Thermo Scientific Fisher Heraeus Biofuge Primo R Centrifuge) ...
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bioRxiv - Cell Biology 2019Quote: ... 50 μg of enriched mitochondria were resolved approximately 6 cm into a pre-cast 4–12% Bis-Tris polyacrylamide gel (ThermoFisher Scientific). The lane was excised and cut in 8 approximately equal chunks and the proteins reduced ...
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bioRxiv - Microbiology 2021Quote: ... samples were unfrozen and aliquots of 6 ml per sample were fixed with Electron Microscopy Sciences 4% Paraformaldehyde (5025999, Fisher Scientific), stained with SYBR™ Gold Nucleic Acid Gel Stain (S11494 ...
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bioRxiv - Biochemistry 2020Quote: ... 500 mM 6-aminohexanoic acid) was added to lysates right before loading samples into a NativePAGE Bis-Tris gel 4-16% (Life Technologies). The proteins were separated according to Wittig et al.32 and transferred onto a PVDF membrane using a standard Tris-glycine transfer buffer with 0.05% SDS ...
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bioRxiv - Immunology 2023Quote: ... a total of 6 – 9 consecutive 10-µm coronal sections were taken from of 4 – 6 equally distanced regions along the length of the spinal cord using a Shandon Finesse 325 microtome (Thermo Scientific). Sections were mounted on Superfrost Plus microscope slides (Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... The isolated primary mouse hepatocytes were plated onto collagen-coated 6-well plates at a density of 500,000 cells/well in 4 ml of DMEM (Thermo Fisher Scientific) plus 10% fetal bovine serum ...
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bioRxiv - Molecular Biology 2023Quote: ... Grids were blotted for 6 s with a blotting force of 6 at 4 °C and 100% humidity and plunged into liquid ethane using a FEI Vitrobot Mark IV (Thermo Fisher). The data were collected with a Glacios Cryo-TEM (Thermo Fisher ...
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bioRxiv - Genomics 2023Quote: ... for at least 6 hrs at 4 °C with rotation in 0.5 % BSA (Jackson Immunology, cat# 001-000-161) in PBS (Gibco, cat# 14190250), followed by blocking with 1% BSA in PBS for 1 hr at 4°C with rotation ...
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bioRxiv - Neuroscience 2024Quote: ... The lentiviral construct of interest was co-transfected with the packaging constructs pCMV-VSV-G and pCMV Gag/Pol at a ratio of 10 µg: 4 µg: 6 µg respectively using Lipofectamine 2000 (60 µL, Invitrogen 11668027). After 4 h ...
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bioRxiv - Immunology 2022Quote: ... Each antibody (2-4 µg per reaction) and IgG control were incubated with Dynabeads (Invitrogen) for at least 4 h at 4C with rotation ...
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bioRxiv - Neuroscience 2019Quote: Freshly dissected mouse brains were quickly frozen in a 2-methylbutane (Fisher Scientific #O3551-4) dry ice bath ...
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bioRxiv - Biochemistry 2020Quote: ... the DNA was digested by addition of 2 µL/4 U Turbo DNase (Ambion, AM2238) for 15 min at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... A 4’-5-diamidina-2-phenylindole (DAPI)-containing mounting medium was used (ThermoFisher, cat. 62248). Images were acquired using a spectral Zeiss LSM800 confocal microscope or Confocal Zeiss LSM980 - AiryScan and analyzed with Fiji software (version 2.0.0) ...
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bioRxiv - Cell Biology 2021Quote: ... A 4’-5-diamidina-2-phenylindole (DAPI)-containing mounting medium was used (ThermoFisher, cat. 62248). Images were acquired using a spectral Zeiss LSM800 confocal microscope and analyzed with Fiji software (version 2.0.0) ...
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bioRxiv - Molecular Biology 2020Quote: ... fixed using 4% paraformaldehyde and blocked with PBS containing 2% bovine serum albumin (BSA) (Invitrogen) and 0.1% normal goat serum (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: ... MCF7 cells were treated with 400 µM 4-thiouridine for 2 hours (4sU, Fisher Scientific). After labeling ...
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bioRxiv - Microbiology 2023Quote: ... The crosslinked membrane was prehybridized at 62°C for 2-4 hours in ULTRAhyb (Ambion) buffer and then labeled probe was added for overnight incubation ...
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bioRxiv - Cell Biology 2023Quote: ... at 4°C containing 2% v/v antibiotic-antimycotic (anti-anti, 15240, Thermo Fisher Scientific). The bone part was cut perpendicular to the longitudinal axis using a bench saw (KS230 ...
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bioRxiv - Neuroscience 2023Quote: Freshly dissected mouse brains were quickly frozen in a 2-methylbutane (Fisher Scientific #O3551-4) dry ice bath ...
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bioRxiv - Biochemistry 2023Quote: ... at 4°C before centrifugation and bead removal using a DynaMag™-2 Magnet (Invitrogen). Supernatants mixed 1:1 with Co-IP elution buffer (2X NuPAGE® LDS ...
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bioRxiv - Systems Biology 2023Quote: ... and incubated at 4°C for 45 min with 2 μg/mL Alexa647 (Life Technologies)-conjugated M1 anti-FLAG (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with following primary antibodies and/or labelling agents: anti-GFP (Invitrogen, #11122, 1:800, 3 days or Invitrogen, #10262, 1:800, 3 days), anti-V5 (Invitrogen ...