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Citations for Thermo Fisher :
2351 - 2400 of 10000+ citations for 2' 3' cGAMP STING based FRET Detection Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... adding 3 mL HPLC grade methanol and 2 mL HPLC grade chloroform (C297-4, Thermo Fisher Scientific), then shaking at 22°C for 1 hr ...
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bioRxiv - Genomics 2023Quote: ... and CRISPRmap library plasmid (2:3:4 ratio by mass) using Lipofectamine 3000 (Thermo Fisher Scientific L3000001) in lentiviral packaging medium (Opti-MEM (Thermo Fisher Scientific 31-985-070 ...
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bioRxiv - Biochemistry 2023Quote: ... cells were incubated with 1.2mM 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT, Thermo Fisher Scientific) solution ...
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bioRxiv - Systems Biology 2023Quote: ... or F-12K medium (Caisson; Hela-S3 and PC-3) supplemented with 2 mM L-glutamine (Gibco), 100 U/mL penicillin ...
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bioRxiv - Molecular Biology 2023Quote: U2OS 2-6-3 cells (Shanbhag et al., 2010) were cultured in McCoy’s 5A (Modified) Medium (Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... incubated for 2–3 minutes (mins) at room temperature in 0.05% (v/v) trypsin (Gibco 25300-054), and tapped to release ...
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bioRxiv - Cell Biology 2023Quote: ... after the first fast centrifugation step platelets were incubated with 3 µM Fura-2 AM (#F1221, Invitrogen) and 0.2% Pluronic F-127 (#P3000MP ...
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bioRxiv - Molecular Biology 2023Quote: ... An Acclaim PepMap 100 trap column (75 μm × 2 cm, C18, 3 μm, 100 Å, Thermo Scientific) was used together with an analytical PepMap RSLC C18 column (150 μm × 15 cm ...
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bioRxiv - Cell Biology 2023Quote: ... DAPI (4’,6-Diamidino-2-henylindole, dihydrochloride) was obtained from Invitrogen (Catalog: D1306, CAS:28718-90-3).
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bioRxiv - Neuroscience 2023Quote: ... The growth medium was changed every 2–3 days and consisted of DMEM/F12 (Fisher Scientific, 11330032) supplemented with 10% fetal bovine serum (Fisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: Total RNAs (2–3 μg) were extracted from tobacco leaves using PureLink Plant RNA Reagent (Invitrogen, #12322012) and used for cDNA synthesis with M-MLV Reverse Transcriptase (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: Proliferation assays were performed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Thermo Fisher Scientific) assay ...
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bioRxiv - Cell Biology 2022Quote: ... Potential intra- and intermolecular hybridization of each potential probe sequence were evaluated using the Multiple Primer Analyzer web tool from Thermo Fisher (sensitivity for dimer detection = 3). The resulting candidate probes ...
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bioRxiv - Cancer Biology 2022Quote: ... To count the apoptotic events cells were treated with a fluorescent dye detecting Casp3/7 activation (CellEvent™ Caspase-3/7 Green Detection Reagent, Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Pathology 2021Quote: ... gondii using 5X MagMAX viral Isolation kit (Applied Biosystems AMB1836-5). Primers and probes for real-time PCR were designed according to the protocol of De Craeye et al ...
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bioRxiv - Developmental Biology 2022Quote: ... and 5 μl of APC-conjugated anti-c-KIT (Invitrogen, CD11705) antibodies for 20 minutes at room temperature with rotation at 10 revolutions per minutes (rpm ...
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bioRxiv - Cell Biology 2023Quote: ... 5 minutes) using a TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems) using specific primers (Applied Biosystems) ...
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bioRxiv - Neuroscience 2020Quote: ... TMT labelled peptides were subjected to TiO2-based phosphopeptide enrichment according to the manufacturer’s instructions (High-Select™ TiO2 Phosphopeptide Enrichment Kit, Thermo Fisher Scientific, A32993). The flow-through and washes from the TiO2 enrichment step were combined ...
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bioRxiv - Biochemistry 2021Quote: ... Total genomic DNA was isolated from a fresh or frozen pellet of 50 mL culture using a CTAB-chloroform/isoamyl alcohol-based protocol [38] or using the commercial PureLink Genomic DNA Mini Kit (Thermo Fisher Scientific®) or the NZY Plant/Fungi gDNA Isolation kit (NZYTech) ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial numbers were determined by spectrophotometry at 600 nm based on a standard curve generated using the LIVE/DEAD BacLight Bacterial Viability and Counting Kit (Molecular Probes, Eugene, OR), as previously described [20 ...
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bioRxiv - Microbiology 2020Quote: ... pastoris was quantified according to a sensitive fluorescence-based method using Molecular Probes™ EnzChek™ Lysozym-Assay-Kit (ThermoFisher Scientific, Katalognummer: E22013). DQ lysozyme substrate (Micrococcus lysodeikticus ...
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bioRxiv - Neuroscience 2020Quote: ... evaporated to dryness and subjected to Fe-NTA-based phosphopeptide enrichment according to the manufacturer’s instructions (High-Select™ Fe-NTA Phosphopeptide Enrichment Kit, Thermo Fisher Scientific, A32992). Phosphopeptides enriched using TiO2- and Fe-NTA-based methods were combined ...
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bioRxiv - Synthetic Biology 2022Quote: ... we developed a vacuum-based plasmid extraction protocol named “TeVacS” on Fluent using the PureLink™ Pro Quick96 plasmid purification Kit (K211004A) (Invitrogen, Carlsbad, CA). Our customized protocol features the use of vacuum manifold for efficient removal of washing buffer and final elution of plasmid DNA into a 96-well plate placed on a 3D-printed holder ...
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bioRxiv - Neuroscience 2023Quote: ... Sample concentrations were quantified based on the standard curve and normalized to total protein obtained through BCA protein assay kit (Thermo Fisher Scientific, 23225) We performed 5-6 independent experiments with 2-3 technical replicates per experiment that were averaged within each experiment.
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bioRxiv - Bioengineering 2019Quote: ... The bionic corals were transferred to 6-well plates filled with 3 mL of ASW medium30 containing broadband antibiotics (penicillin/streptomycin, Gibco) at a concentration of 1:1000 ...
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bioRxiv - Molecular Biology 2020Quote: ... We then diluted these saturated cultures 1:20 in sterile water and then inoculated 3 µL into 96-well humidified plates (Nunc Edge Plates (Thermo Scientific ...
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bioRxiv - Immunology 2019Quote: ... HEK-293T cells seeded at 2×105/well in 12 well plates and transfected with the indicated miR-722 overexpression and the RAC2 3’UTR reporter plasmids with Lipofectamine 3000 (Invitrogen). Cells were harvested and lysed at 48 h post transfection and luciferase activity was measured with Dual Glo-Luciferase reagent (Promega ...
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bioRxiv - Immunology 2021Quote: ... To obtain Mfs purified monocytes were plated on 24 well plates 3 × 105 cells/well and cultured for 7 days in Macrophage-SFM (Gibco, Life Technologies ...
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A sound strategy for homology modeling-based affinity maturation of a HIF-1α single-domain intrabodybioRxiv - Biochemistry 2020Quote: The HIF-1α-PAS-B domain was coated at 3 μg/ml onto 96-well microtiter plates (Thermo Fisher Scientific) and incubated overnight at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... Following another 3 washes with PBS plates were incubated for 1h with secondary goat anti-guinea pig-AlexaFluor488 (Invitrogen, UK) at 1:500 in PBS-0.5%BSA ...
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bioRxiv - Genomics 2021Quote: Organoids seeded in 6-well plates were harvested after 3-days incubation with WYE-132 in RIPA buffer (Thermo Scientific) supplemented with protease inhibitors (Complete Mini ...
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bioRxiv - Microbiology 2022Quote: ... Samples were run in technical duplicates on 96-well plates using a Quantstudio 3 thermocycler (Applied Biosystems, Foster City, CA). Standards were prepared using linearized plasmids ...
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bioRxiv - Cell Biology 2022Quote: ... organoid fragments collected from 3 wells of a 24 well plate were incubated with TrypLE express cell dissociation enzyme (ThermoFisher) supplemented with Y-27632 (10 µM ...
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bioRxiv - Microbiology 2022Quote: A total of 1.5 µg/well of VA1 IVT RNA was transfected into BHK-21 cells in 12-well plates using 3 µL/well of Lipofectamine MessengerMax (Invitrogen) and following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... 2500 Vero-E6 (12 μl/well) or 10000 Calu-3 (12 μl/well) were seeded in 384-well white optical-bottom tissue culture plates (Nunc) with the Multidrop Combi liquid handling instrument (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 mg/l streptomycin and 3% bovine serum albumin (BSA) were pipetted into a 96-well optical bottom plate (Nunc) and centrifuged at 1,000 rpm for 3 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Purified OPCs were then seeded onto poly-D-lysine-coated 12-multiwell plates at a density of 3 × 104/cm2 in OPC proliferation medium composed of Neurobasal medium (Gibco), 2% B21 (Miltenyi biotec) ...
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bioRxiv - Microbiology 2020Quote: ... or 1×104 Calu-3 (12 μl/well) were seeded in 384-well white optical-bottom tissue culture-treated plates (Nunc) with a Multidrop Combi liquid handling instrument (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... 24 hours before transfection 1.5 × 105 cells were seeded in a 6-wells plate with 3 mL of antibiotic-free medium (DMEM (Gibco™) high glucose ...
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bioRxiv - Molecular Biology 2022Quote: ... The Gibson reaction mix was transformed into Mach1 competent cells (4 μl Gibson into 40 μl cells, 288 reactions in 3×96-well plates) following manufacture’s protocol (Invitrogen C862003). After transformation ...
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bioRxiv - Cancer Biology 2022Quote: ... 2.5×105 CAR or NT-T cells were incubated with SSEA-4 and SSEA-3 (#GLY131 and #GLY122, respectively, Elicityl-Oligotech) at 200 μM for 48 h in 24 well plate (Nunc). 5×105 CAR and NT-T cells CFSE-labeled were incubated with or without 1×106 OVCAR4 and Caov-3 cells for 48 h in RPMI-10% FBS-Pen/Strep and L-Glut medium in 10 cm cell culture dishes (Nunc) ...
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bioRxiv - Cell Biology 2022Quote: ... after the last washing step RPMI was replaced with RPMI containing 3% BSA to activate sporozoites and samples were transferred to a 96-well plate with optical bottom (Nunc). To allow sporozoites to attach ...
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bioRxiv - Bioengineering 2023Quote: ... pcDNA-uAb (500 ng) plasmids were transfected into cells as triplicates (3×105/well in a 24-well plate) with Lipofectamine 2000 (Invitrogen) in Opti-MEM (Gibco) ...
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bioRxiv - Neuroscience 2023Quote: ... The plate was sealed with adhesive film and qPCR was performed in a light cycler (Quant Studio 3, ThermoFisher Scientific) using the follow sequence ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was extracted from brain punches as well as HBEC- 5i and bEnd.3 cells in 6-well plates using TRIzol (Invitrogen) homogenization and phase separation with chloroform ...
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bioRxiv - Biochemistry 2023Quote: ... an equivalent quantity of SLO protein or the d3_m construct (ca. 3 ug) was immobilized onto a MaxiSorp plate (Thermo Scientific), which was subsequently incubated with either 0.4 ug nAb (followed by serial half-dilution) ...
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bioRxiv - Biochemistry 2023Quote: ... and SARS-CoV-1 HexaPro S) at 3 µg/mL were placed into 384-well Nunc Maxisorp plates (ThermoFisher, 464718) in 1x TBS and incubated for 1 hour at 37°C followed by slap drying and blocking with 80 µL of Casein for 1 hour at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... The 10µl of cells suspension infected with virus was plate micromass cultures (3-4 spots per 35mm culture dish, NUNC #150318) at a density of 2 x 107 cells/ml ...
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bioRxiv - Immunology 2024Quote: Qualitative IgG binding ELISAs were carried out by coating PvDBP-RII or subdomain 3 on Maxisorp flat-bottom 96-well ELISA plates (Nunc) at 2 µg/mL in 50 µL at 4 °C overnight ...
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bioRxiv - Neuroscience 2024Quote: 3 to 6 dpf-zebrafish larvae were placed in a 48-well plate (one larva per well, Fisher Scientific, USA) filled with embryo medium ...