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Citations for Thermo Fisher :
2251 - 2300 of 10000+ citations for 2' 3' cGAMP STING based FRET Detection Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 800 μL of the supernatant was transferred to a 2 mL vial for detection by GCMS-QP2010 Ultra (Thermo Scientific, Massachusetts, USA). The specific parameters were as follows ...
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bioRxiv - Cancer Biology 2023Quote: ... washed twice with PBS (1X) and incubated for 2 hours at 37°C with secondary detection antibodies (1:500) and phalloidin (1: 3000) (Invitrogen, Waltham, MA) for cytoskeleton staining ...
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bioRxiv - Microbiology 2021Quote: Hemolytic index was measured using 1.5% BHI agar plates supplemented with 5% defibrinated sheep blood (Thermo Scientific). After overnight incubation at 30°C ...
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bioRxiv - Biochemistry 2020Quote: ... The ThT fluorescence signal was measured every 5 min using the Varioskan plate reader (Thermo Scientific, Inc.) or FLUOstar Omega plate reader (BMG Labtech ...
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bioRxiv - Cancer Biology 2022Quote: ... MS-5 cells were seeded into 0.2% gelatin coated 96 well-flat bottom plates (Thermo Fisher, 167008) at a density of 4-5×105 cells per well in Myelocult medium (H5100 ...
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bioRxiv - Microbiology 2021Quote: ... 5∼15 ng DNA per reaction in 384-well Q-PCR plates (Applied Biosystems™ Cat. # 4483285) with total 12 μL reaction ...
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bioRxiv - Molecular Biology 2020Quote: ... samples were desalted using HyperSep Filter Plates with a 5-7 μL bed volume (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Transfection with 5 ug total DNA in a 6 well plate was performed using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... plates were washed thrice for 5 minutes with 200 µL 1X PBS-T (ThermoFisher Scientific, Cat# 28352), and then blocked overnight at 4°C with 200 µL of 2% BSA in PBS-T to prevent non-specific binding ...
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bioRxiv - Microbiology 2023Quote: ... 5 µL of conjugation reactions were pipetted onto a rectangular LB-agar plate (Thermo Fisher Scientific #267060) containing 0.3 mM DAP and incubated at 37 °C for 5 h ...
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bioRxiv - Neuroscience 2023Quote: ... hiPS cells were cultured on plates coated with vitronectin (5 μg ml−1; Thermo Fisher Scientific, A14700) in Essential 8 medium ...
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bioRxiv - Immunology 2024Quote: ... 6-well culture plates) for 8 days at 37°C and 5% CO2 in RPMI1640 (Life technologies) supplemented with 10% FBS ...
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bioRxiv - Immunology 2019Quote: ... Murine IL-2 ELISA’s were performed using the mouse IL-2 ELISA kit (Thermo Fisher Scientific).
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bioRxiv - Immunology 2020Quote: ... IL-2 was measured from culture supernatants using a mouse IL-2 ELISA kit (Thermo Scientific).
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bioRxiv - Immunology 2021Quote: ... plates were washed and blocked for 2 hours with Casein Buffer (Thermo Fisher Scientific, Cat# 37528). Then ...
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bioRxiv - Bioengineering 2021Quote: ... cells were seeded at 2 × 105 cells/well in round-bottom 96-well plates (Fisher Scientific) in IMDM with 10% FBS and 2% penicillin/streptomycin (Life Technologies) ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293 cells were seeded at 2 ×105 cells per well in six well plates (Thermo Fisher) in DMEM/10% FBS ...
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bioRxiv - Evolutionary Biology 2020Quote: All experiments were performed in 96 deep-well plates (maximal volume: 2 ml, Thermo Scientific Nunc) with minimal medium for Azospirillium brasilense (MMAB)48 without biotin and using glucose (5 g l-1 ...
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bioRxiv - Evolutionary Biology 2020Quote: All experiments were performed in 96 deep-well plates (maximal volume: 2 ml, Thermo Scientific Nunc) with minimal medium for Azospirillium brasilense (MMAB)48 without biotin and using glucose (5 g l-1 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were seeded at 2 = 105/mL in 96 well plates (Thermo scientific 167006 Nunclon delta surface) and incubated for 24 h at 37°C and 5% CO2 (Nuaire ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Caco-2 cells were seeded into Nunclon™ Delta 96-Well MicroWell plates (#167008, ThermoFisher Scientific) at a density of 1.5 x 104 cells/cm2 and were maintained for seven days in complete medium ...
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bioRxiv - Molecular Biology 2021Quote: U-2 OS and HEK293T cells were cultured on adherent plates in DMEM high glucose (Gibco) supplemented with 10% fetal bovine serum (Gibco) ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 μg of reporters were transfected into cells in 6 well plate using lipofectamine 3000 (Invitrogen) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2022Quote: ... Live cultures or thawed cells were either expanded as floor plate progenitors (following Brundin approach; Floor Plate Progenitor Kit expansion protocol (ThermoFisher; A3165801) or terminally differentiated to DA neurons with all prior factors except in neurobasal base medium after day 25.
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bioRxiv - Immunology 2023Quote: ... A 10 μl supernatant sample was mixed with 90 μl substrate solution containing 0.5 mg/ml of 4-methylumbelliferyl N,N’-diacetyl-β-D -chitobioside in an assay buffer provided as part of the kit and in a 96-well plate (black/clear bottom plate, Thermo Fisher). The reaction was incubated at 37 °C for 30 min and then stopped by adding 100 μl stop solution ...
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bioRxiv - Physiology 2021Quote: ... HUVECs were washed 2 × with D-PBS and loaded with DAF-FM™ diacetate (4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate; Molecular Probes, Invitrogen) to a final concentration of 1 µM in KRH buffer and incubated at 37°C for 45 minutes protected from light ...
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bioRxiv - Neuroscience 2022Quote: ... and 12 old (21 months) male C57BL/6 animals (combined over 2 independent experiments) were intraperitoneally injected with 5-ethynyl-2’- deoxyuridine (EdU) (Fisher Scientific, A10044) (resuspended in PBS at 5 mg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were loaded onto a 2 cm pre-column (C18, 5 μm, 100 A°, 100 μ, 2 cm Nano-viper column # 164564, Thermo Scientific) at 5 μl/minute flow rate using loading pump for about 5 minutes and then resolved the peptides on a 50 cm analytical column (C18 ...
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bioRxiv - Cell Biology 2022Quote: ... 2’,7’-Bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM) was purchased from Molecular Probes (Invitrogen, Carlsbad, CA, USA). Fluorescein isothiocyanate (FITC)- and tetramethylrhodamine (TRITC)-conjugated goat anti-mouse and rabbit IgG antibodies were purchased from Jackson ImmunoResearch (West Grove ...
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bioRxiv - Biophysics 2024Quote: ... Cell detachment for cryopreservation or experiments was achieved through 30 minutes of incubation with 5 mM EDTA in PBS without Ca+2 and Mg+2 (PBS-/-) followed by TrypLE Express dissociation agent (Gibco, 12605-010) for five minutes at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: RNA was extracted from 5 pooled larvae aged 5 dpf using the PicoPure RNA Isolation Kit (Applied Biosystems). After quality control ...
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bioRxiv - Neuroscience 2022Quote: ... TaqMan RT-qPCR assays were duplicated for each gene on cDNA samples in 384-well optical plates using an ABI Prism 7900 Sequence Detection system (Applied Biosystems, Life Technologies, Waltham, MA, USA). For each 10μL TaqMan reaction ...
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bioRxiv - Cell Biology 2022Quote: ... The C2C12 cells were transfected with 2 μg plasmids (L-Cas9n-EGFP:R-Cas9n-puro=3:1) by electroporation (1650 v/10 ms/3 pulses) with the Neon Transfection System Kit (Thermo Fisher Scientific). The cells were seeded overnight to 24-well plates containing an antibiotic-free complete mediums ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 μl of sample was saved for 3′ -biotin labeling for visualization using Pierce RNA 3′ End Biotinylation Kit (Life Technologies 20160) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Signal detection was performed with SuperSignal™ West Femto Maximum Sensitivity Substrate chemiluminescence detection system (ThermoFisher, France). Densitometric analysis was achieved using ImageJ software ...
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bioRxiv - Molecular Biology 2022Quote: ... x 5 mm 5 μm 100 Å and an Acclaim PepMap RSLC 75 μm x 50 cm 2 μm 100 Å (Thermo Scientific). The columns were installed on an Ultimate 3000 RSLCnano system (Dionex) ...
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bioRxiv - Biochemistry 2024Quote: ... x 5 mm 5 μm 100 Å and an Acclaim PepMap RSLC 75 μm x 50 cm 2 μm 100 Å (Thermo Scientific). The columns were installed on an Ultimate 3000 RSLCnano system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: Total RNA extracted from BoDV-2-infected Vero cells was ligated with either 5’ adaptor oligoRNA (5’-CGACUGGAGCACGAGGACACUGACAUGGACUGAAGGAGUAGAAA (Thermo Fisher Scientific, USA; #L150201)) or 3’ adaptor oligoRNA (5’-GAAGAGAAGGUGGAAAUGGCGUUUUGG ...
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bioRxiv - Physiology 2020Quote: ... 1 h with oxygenated water 3% (Tyramide Superboost Kit, B40922, Thermo Fisher, USA) and ...
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bioRxiv - Cell Biology 2019Quote: Calcium transient was measured with Rhod-3 Calcium Imaging Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... mid-log cultures (3×109 cells) using the RiboPure™-Bacteria Kit (Ambion) and treated with DNase (Ambion ...
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bioRxiv - Cancer Biology 2022Quote: ... using the Pierce RNA 3′ End Desthiobiotinylation kit (Pierce/Thermo Fisher Scientific, 20163), and were purified using the RNA cleanup protocol (QIAGEN) ...
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bioRxiv - Plant Biology 2022Quote: Using Pierce RNA 3’ end desthiobiotinylation kit (Thermo Fisher Scientific, Waltham, MA, USA), 50 pmol of PSTVd dimer RNA was labeled following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Calcium transient was measured with Rhod-3 Calcium Imaging Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... oligonucleotides labeled with biotin by Biotin 3’ DNA labeling kit (Thermo Fisher, 89818) were used to detect precursor rRNA ...
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bioRxiv - Cancer Biology 2024Quote: ... Following fixative treatment then staining with the Hema 3 kit (Thermo Fisher Scientific), quantity of cells that invaded through the VitroGel and across the Boyden chamber membrane was quantified ...
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bioRxiv - Plant Biology 2019Quote: ... the full-length GAD2 was also amplified using primer set GAD2_forward2 (5’-CACCATGGTTTTGACAAAAACCGC-3’) and GAD2_reverse and cloned into pENTR/D-TOPO vector via directional cloning (Invitrogen), followed by a LR reaction recombinant into pMDC3240 ...
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bioRxiv - Immunology 2021Quote: ... RT-PCR was performed on QuantStudio™ 3 and 5 Real-Time PCR System (Thermo Fisher Scientific, USA) using KAPA SYBR®Fast qPCR Master Mix (Kapa Biosystems).
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed 3 x 5 min and incubated for 10 min with DAPI (1/2000, Invitrogen #D1306) diluted in PBT + 0.5% BSA ...
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bioRxiv - Cancer Biology 2022Quote: ... for 5 minutes followed by a wash with chilled PBS and blocked with 3% goat serum (Thermo Scientific) for 1h at room temperature ...