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Citations for Thermo Fisher :
2301 - 2350 of 10000+ citations for Mouse IL 17D ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed and then stained with triple-washed anti-IgG magnetic beads (Untouched Mouse T Cells Kit, Thermo Fisher) rotating at 4°C for 30 min ...
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bioRxiv - Neuroscience 2022Quote: Using a subcloned and sequenced PCR fragment (162 bp – Table S1) of the mouse 5-HT2AR cDNA (Zero Blunt TOPO PCR Cloning kit, Invitrogen), high specific-activity RNA probes were produced from a linearized plasmid (HindIII ...
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bioRxiv - Microbiology 2022Quote: Chemokine and cytokine profiles of serum and lung samples were assessed using Immune Monitoring 48-plex mouse ProcartaPlex Panel kits (Invitrogen). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... containing a 1/250 dilution of Alexa 488-conjugated anti-Spike antibody (GTX632604 conjugated using the Zenon Alexa Fluor 488 Mouse IgG Labeling Kit, ThermoFisher) and washed 4 times in FACS buffer ...
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bioRxiv - Physiology 2021Quote: ... RNA samples were prepared from the mouse tissues using the PureLink RNA Mini Kit (Invitrogen by ThermoFisher Scientific, catalog #12183025). Briefly ...
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bioRxiv - Immunology 2020Quote: Peripheral T cells were isolated from mouse spleen by a CD4+ T-cell negative (Stem Cell) or positive selection kit (Invitrogen). Enriched CD4+ T cells were further sorted for either YFP+ (Foxp3+ ...
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bioRxiv - Neuroscience 2020Quote: ... were designed against the mouse Auts2 coding sequence using the BLOCK-iT Pol II miR RNAi Expression Vector Kit guidelines (Invitrogen). Auts2 miRNA constructs were subcloned into the pCL20c-trL7 ...
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bioRxiv - Immunology 2020Quote: Splenic T cells from MRL/Lpr mice were purified with Dynabeads™ FlowComp™ Mouse Pan T (CD90.2) Kit and dissocated from beads as per manufacture’s instructions (ThermoFisher). Purified T cells were staind with PE-conjugated anti-CD138 antibody ...
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bioRxiv - Cell Biology 2019Quote: ... For direct labeling of the anti-CC10 antibody Zenon Alexa Fluor 546 mouse IgG1 kit was used according to the manufacturer’s recommendations (Invitrogen, 25004). Stained cryosections were analyzed on an epifluorescence microscope (Zeiss Imager.M2 ...
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bioRxiv - Immunology 2021Quote: ... S98I to the SIRPα domain which was then subcloned and fused to mouse IgG1 N297A via GeneArt Seamless Plus Cloning Assembly Kit (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Barcoded libraries were prepared using the Ion AmpliSeq Transcriptome Mouse Gene Expression Kit as per the manufacturer’s protocol and sequenced using an Ion S5 system (ThermoFisher Scientific). Differential gene expression analysis was performed using the Transcriptome Analysis Console (TAC ...
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bioRxiv - Immunology 2021Quote: MesLNs were collected and CD4+ T cells isolated using the Dynabeads™ Untouched™ Mouse CD4 Cells Kit (Thermo Fisher) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... naïve OT-I and OT-II cells were obtained by negative selection using the Dynabeads Untouched Mouse CD4 cell kit (ThermoFisher). Flow cytometry indicated >85% enrichment in all cases ...
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bioRxiv - Immunology 2021Quote: MRL/Lpr mice splenic T cells were purified with Dynabeads™ FlowComp™ Mouse Pan T (CD90.2) Kit and dissociated from beads according to manufacturer’s instructions (ThermoFisher). After staining purified T cells with PE anti-CD138 antibody ...
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bioRxiv - Immunology 2022Quote: ... CD4+CD25+ natural Tregs were isolated from the splenocytes using the DynabeadsFlowComp Mouse CD4+CD25+ Treg cells Kit (11463D, Invitrogen) following the manufacturer’s instructions and as previously described (37)(38) ...
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bioRxiv - Molecular Biology 2023Quote: ... Fab were generated from monoclonal antibodies using the Pierce Mouse IgG1 Fab and F(ab’)2 Preparation Kit (Thermo Fisher). Antibodies were first digested into Fab in a Zeba Desalt Spin Column (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: Total RNA from isolated HeLa nucleoli was depleted of rRNA by the RiboMinus™ Human/Mouse Transcriptome Isolation Kit (Invitrogen). RNA was converted to double stranded cDNA using random hexamer with the NEBNext Ultra RNA First Strand and NEBNext Ultra RNA 2nd Strand Synthesis Kits (NEB) ...
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bioRxiv - Pathology 2024Quote: ... A mouse anti-human CD11b activated labeled with FITC (clone CBRM1/5, eBioscience) and elastin (EnzChek Elastase Assay Kit, ThermoFisher) were used in association with a rat anti-mouse LY6G antibody for the evaluation of neutrophils activation in cytometry ...
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bioRxiv - Immunology 2024Quote: ... naïve CD4+ T cells were enriched from homozygous TCR-transgenic OT-II (CD45.1/.2) donors using the Dynabeads Untouched™ Mouse CD4 Cells kit (Invitrogen). 1 x 105 OT-II cells were transferred intravenously (i.v. ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from either cultured neurons (>1.5×106) or mouse spinal cord tissue (10-20 mg) using the PureLink RNA Mini kit (Thermo Scientific) and treated with DNase I (NEB ...
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bioRxiv - Cancer Biology 2023Quote: 20 μg protein per sample was used for the IFN-gamma mouse ProcartaplexTM Simplex High Sensitivity kit (Thermo Fisher Scientific) and the protocol was followed as per the product manual ...
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bioRxiv - Physiology 2023Quote: ... was incubated with 5 µl Zenon component A solution for five minutes and then with 5 µl Zenon component B solution (ZenonTM Mouse IgG1 Labeling Kit Z-25006, Alexa Fluor 568, Invitrogen). PBS with 0.1% triton was added to the Zenon-labeled antibody mix to a final 1:75 dilution ...
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bioRxiv - Cell Biology 2023Quote: A PCR product encoding mouse RAB18 was subcloned into an intermediate TOPO vector using a TOPO PCR Cloning Kit (ThermoFisher) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... both following manufacturer’s suggested protocols for the Ion AmpliSeq Transcriptome Mouse Gene Expression Kit (Thermo Fisher Scientific, San Jose, CA). Differentially expressed genes (DEGs ...
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bioRxiv - Physiology 2023Quote: First and second-order mesenteric arteries from each mouse were pooled and placed into lysis buffer from the RNAqueous Micro Kit with DNase from Ambion Inc ...
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bioRxiv - Immunology 2023Quote: ... Mouse FM expression of IL1B and KC was measured by RT-qPCR using the SuperScript II kit (Invitrogen; Waltham, MA) and the Kapa SYBR Fast qPCR kit (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... We followed the kit protocol except for conjugating 5µg of anti-total RNAPII to sheep anti-mouse dynabeads (Life Technologies). Sequencing libraries were generated using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina.
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bioRxiv - Cell Biology 2024Quote: We established mouse iPSC lines (CD1 iPSC or ntdTomato iPSC) using Sendai virus-mediated reprogramming kit: CytoTune2.0 (Thermo Fisher, A16517) and followed manufactured protocol as described before24 ...
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bioRxiv - Immunology 2024Quote: Cytokine profiling of plasma and brain tissue homogenates was performed using the Th1/Th2 Cytokine & Chemokine 20-Plex Mouse ProcartaPlex kit (EPX200-26090-901; Invitrogen) and measured Th1/Th2 ...
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bioRxiv - Immunology 2021Quote: ... containing the cytokines IL-4 (500 U/mL) and GM-CSF (800 U/mL) (both Gibco) for differentiation into DCs ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein concentration was evaluated using the Modified Lowry Protein Assay (Thermo Fisher Scientific, Rockford, IL, USA). Samples were prepared for SDS-PAGE in Laemmli sample buffer (Bio-Rad ...
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bioRxiv - Pathology 2022Quote: Total RNA was extracted from the heart using TRIzol reagent (Thermo Fisher Scientific, Rockford, IL, USA) as reported previously.8 Total RNA was reverse transcribed into cDNA using random primers (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... The protein band was detected using Super Signal West Pico Chemiluminescent Substrate (Thermo Scientific, Rockford, IL).
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bioRxiv - Molecular Biology 2021Quote: The LysoTracker Red assay was used to follow the manufacturer’s protocol (LifeTechnology, ThermoFisher, Rockford, IL, USA). LysoTracker Red (50 nM ...
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bioRxiv - Molecular Biology 2021Quote: ... 5% sodium dodecyl sulfate (SDS) in 50 mM triethylammonium bicarbonate (TEAB, Thermo Fisher Scientific, Rockford, IL), pH=8.0) ...
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bioRxiv - Cell Biology 2022Quote: ... Cell lysates were prepared in the modified RIPA buffer containing protease inhibitors (Thermo Scientific, Rockford, IL). Equal amounts of proteins ...
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bioRxiv - Biochemistry 2019Quote: Primary enriched CM and hiPSC-CM were lysed in 1X Invitrosol (Thermo Fisher Scientific, IL, USA), 100 mM ammonium bicarbonate (NH4HCO3) ...
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bioRxiv - Immunology 2019Quote: ... The protein concentrations were determined by the bicinchoninic acid assay (BCA, Pierce Biotechnology, Thermo Scientific, IL). Further processing was adapted from a filter-aided sample preparation (FASP ...
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bioRxiv - Physiology 2020Quote: ... The PCR amplifications were performed with SYBR green qPCR master mix (#K0252, Thermo Scientific, Rockford, IL). Total RNA was prepared from the frozen whole uterine and placental tissues ...
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bioRxiv - Biophysics 2019Quote: ... proteins were purified using HisPur Cobalt Resin according to the manufacturer’s protocol (Thermo Scientific, Rockford, IL.) and Ulp-1 protease (Ubl-specific protease 1 ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were collected and used for cytokine measurement with IL-18 (Invitrogen; sensitivity 19.0 pg/mL), IL-1β (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... Gels were transferred onto Biodyne B nylon membranes (0.45 μm; Thermo Fisher Scientific, Rockford, IL, USA) in 0.5X TBE buffer (60V for 30 min) ...
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bioRxiv - Biochemistry 2021Quote: ... that contained 1mg/ml Texas Red-dextran (10,000 MW, Lysine Fixable, Thermo Fisher Scientific, Rockford, IL) at 37°C for 6-24 hr ...
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bioRxiv - Cell Biology 2021Quote: ... M1 tri-Ub was purified to homogeneity by Ni2+-NTA affinity column (Thermo Scientific, Rockford, IL) chromatophraphy ...
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bioRxiv - Cell Biology 2021Quote: ... Protein content was determined using the bicinchoninic acid assay (BCA) (Thermo Fisher Scientific, Rockford, IL, USA).
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration was determined by the Bicinchoninic acid method (Pierce, ThermoFisher Scientific Fisher, Rockford, IL, USA). Aliquots of lysates containing 30 μg of protein were separated on SDS-PAGE and transferred to Immobilon-P membranes ...
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bioRxiv - Cell Biology 2021Quote: Samples were analyzed on Orbitrap Fusion Lumos Plus mass spectrometers (Thermo Fisher Scientific, Rockford, IL, USA) coupled with an Easy-nLC 1000 nanoflow LC system (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were stained with LIVE/DEADTM fixable Aqua dead cell stain (Thermo Fisher Scientific, Rockford, IL) for 30 min at 4oC ...
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bioRxiv - Biochemistry 2022Quote: ... Using Slide –A-Lyzer –Minidialysis units with a 10,000 Dalton MWCO(Thermo Scientific, Rockford, IL, USA) proteins were renatured by dialyzing out urea in a step wise manner from 6 M in steps of two at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... in a 1:1 ratio and maintained in R10 + IL-2 [RPMI 1640 (Thermo Fisher Scientific) supplemented with 10% heat-inactivated FBS (Thermo Fisher Scientific) ...