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Citations for Thermo Fisher :
2501 - 2550 of 10000+ citations for Mouse IL 17D ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... IL-6 (assay ID: HS00985639_m1) and TNF-α: (assay ID: HS00174128_m1) following manufacture’s protocol (Thermo Fisher Scientific). 18s (assay ID ...
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bioRxiv - Cell Biology 2020Quote: ... secondary antibody (diluted 1:10,000) and SuperSignal® West Femto Maximum Sensitivity Substrate (Thermo Scientific, Rockford, IL).
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bioRxiv - Biophysics 2020Quote: ... or Zeba micro spin™ desalting colums 0.5 ml (7000 MWCO, Thermo Fisher Scientific, Rockford, IL, USA) were used ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was reverse transcribed with the SuperScript IV First-Strand Synthesis System (Thermo Fisher Scientific, Rockford, IL). cDNA was PCR amplified using the mEX11F and mEX13R primers and subjected to Sanger sequencing.
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bioRxiv - Biochemistry 2021Quote: ... 0.1% SDS) supplemented with 1X Halt protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific, Rockfold, IL, USA) and 1mM phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Molecular Biology 2020Quote: Chromatographic separation was achieved on an Accucore C30 column (2.6µm, 3mm x 150mm, Thermo Scientific, Rockford, IL) with linear gradient elution consisting of mobile phases A (water/acetonitrile = 40:60 ...
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bioRxiv - Cancer Biology 2020Quote: ... Calibration was performed before each analysis using a positive calibration mix (Piercenet – Thermo Fisher, Rockford, IL, USA). Limits of detection (LOD ...
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bioRxiv - Synthetic Biology 2019Quote: The optical density was measured at 600 nm using a spectrophotometer (GENESYS 30, Thermo Scientific, IL, USA). The dry cell mass was obtained by multiplication of the optical density of culture broth with a pre-determined conversion factor ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were homogenized and resuspended in radioimmunoprecipitation assay (RIPA) lysis buffer (Thermo Fisher Scientific, Rockford, IL, USA) containing a cocktail of protease inhibitors (Roche ...
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bioRxiv - Physiology 2019Quote: ... Gels were washed and proteins were stained with Imperial Protein Stain (Thermo Fisher Scientific, Rockford, IL, USA) for 2 hours ...
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bioRxiv - Immunology 2019Quote: ... IL-2 mRNA amounts were determined with a SYBR Green PCR master mix from Life Technologies (4344463) relative to mRNA for β-2 microglobulin on a DNA Engine Opticon II System (Bio-Rad ...
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bioRxiv - Physiology 2019Quote: ... The quantity of the RNA was determined on a Nanodrop ND-1000 (Thermo Scientific, Rockford, IL, USA). Reverse-transcriptase PCR was performed using the Multiscribe Reverse Transcriptase method (Applied Biosystems ...
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bioRxiv - Cell Biology 2019Quote: ... and eluted with imidazole (gradient 10–200 mM) and dialyzed (prod. no. 68100, Thermo Scientific, Rockford, IL). In the case of Sec14 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Total protein content in the homogenate was estimated using the BCA assay (Thermo Fisher Scientific, Rockford, IL), according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: ... The blots were then developed using Super Signal West Pico Chemiluminescent Substrate (Thermo Scientific, Rockford, Il, USA) and signals were captured by using high sensitive CCD camera-based imager (BioSpectrum Imaging System) ...
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bioRxiv - Physiology 2021Quote: ... The total protein was inoculated with anti-HA magnetic beads at 4 C (Thermo Scientific, Rockford, IL) overnight ...
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bioRxiv - Synthetic Biology 2021Quote: The optical density was measured at 600 nm using a spectrophotometer (GENESYS 30, Thermo Scientific, IL, USA). The dry cell mass was obtained by multiplication of the optical density of culture broth with a pre-determined conversion factor ...
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bioRxiv - Pathology 2021Quote: ... hMADS adipocytes were differentiated in 2-well Lab-Tek Chamber Slides (Nalge Nunc International, Naperville, IL, USA), washed with PBS pH 7.4 and fixed with 10% paraformaldehyde in PBS for 10 min at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... blocked and permeabilized in a 10% BSA solution with Triton- X (Thermo Fisher Scientific, Rockford, IL, USA). Subsequently ...
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bioRxiv - Bioengineering 2022Quote: Samples were denatured and reduced using 5× Lane Marker Reducing Sample Buffer (Thermo Scientific, Rockford, IL, US), boiled at 95°C for 10 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... Heat denatured protein samples were separated using a 4–12% NuPAGE gel (Thermo Scientific, Rockford, IL, USA). Proteins were transferred to PVDF membranes at 30 volts for 90 minutes ...
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bioRxiv - Microbiology 2022Quote: ... Eight drops of the suspension (2 µl) were placed on Polysine slides (Thermo Scientific/Pierce, Rockford, IL), dried ...
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bioRxiv - Physiology 2022Quote: ... and then stained for markers at 5 μg/ml for IL-17A-FITC (Thermofisher, 11-7177-81) and IFNg-PE (Thermofisher ...
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bioRxiv - Bioengineering 2024Quote: ... T-cell cultures were supplemented with 200 U/mL IL-2 (Cat # 200-02, Peprotech (Thermo Fisher), Cranbury ...
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bioRxiv - Neuroscience 2024Quote: ... cells were fixed and immunostained for Iba-1 and cleaved IL- 1β (1:100, PA5-105048, Invitrogen) or CD68 and cleaved caspase 1 (1:200 ...
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bioRxiv - Cell Biology 2023Quote: ... followed by permeabilization for 3 min with 0.15% Surfact-Amps X-100 (28314, Thermofisher Scientific, Rockford, IL) in PBS ...
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bioRxiv - Biophysics 2023Quote: ... human IL-2 (Cat# PHC0026) and CellTrace™ CFSE (Cat# C34570) were purchased from ThermoFisher (Waltham, MA). Bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2023Quote: ... the concentration of peptides was measured using Pierce Quantitative fluorometric peptide assay (ThermoFisher Scientific, Rockford, IL, USA).The resulting peptides were sequenced on a Q-Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... IL-4 and IFN-ᵧ in supernatant was measured according to the manufacturer’s protocol (all Life Technologies).
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bioRxiv - Cell Biology 2023Quote: ... following chemiluminescent detection (SuperSignal® West Pico PLUS and West Femto Chemiluminescent Substrate (Thermo Scientific, Rockford, IL).
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bioRxiv - Cancer Biology 2023Quote: ... ANBL-6 cells were also supplemented with 5 ng/ml IL-6 (Thermo Fisher Scientific, Cat# 206IL010).
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bioRxiv - Physiology 2023Quote: ... membrane was washed and developed using a SuperSignalTM west pico plus chemiluminescent substrate (Thermo Scientific, IL, USA). The blot was developed in iBright 1500 imaging system (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Some of the blots were then stripped (Restore PLUS Western Blot Stripping Buffer; Thermo Scientific, Rockford, IL) and reblotted if needed.
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bioRxiv - Cancer Biology 2024Quote: ... qPCR quantification of miRNA expression was performed using TaqMan MicroRNA® Assays (Thermo Scientific, Rockford, IL, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... The expression of genes was studied using SYBR green method (Finnzymes F410L, Thermo scientific, Rockford, IL, USA). The Ct values were normalized with actin and the fold differences were calculated using 2-ΔΔCt method.
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bioRxiv - Neuroscience 2024Quote: ... 1:400 donkey raised rabbit secondary antibody conjugated with Alexa Fluor 488 (Thermo Fisher Scientific, Rockford, IL) and DAPI were incubated for 24 hours at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... stained with fluorophore-labeled secondary antibodies (Alexa 488 Donkey anti-Mouse, Alexa 555 Donkey anti-Mouse, Alexa 647 Donkey anti-Mouse, Alexa 488 Goat anti-Rabbit; Invitrogen, Carlsbad, CA) and DAPI (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Coronal sections 50 um thick were immunostained for tyrosine hydroxylase (primary: mouse anti-TH, Millipore #MAB318; secondary: goat anti-mouse 647, Invitrogen #A-21236 or goat anti-mouse 405, Invitrogen #A-31553), tryptophan hydroxylase 2 (primary ...
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bioRxiv - Cell Biology 2020Quote: Whole protein extracts were prepared from JJN3 pellets (4°C, 150 x g, 5 min) according to the cell extraction protocol for ELISA sample preparation by ThermoFisher Scientific ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: Serine protease inhibition assays were performed at 37°C by using a thermostated Multiskan GO ELISA plate reader (Thermo Scientific). Serine proteases and their corresponding substrates (in brackets ...
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bioRxiv - Immunology 2021Quote: ... Samples were washed 3 times with PBS-T and then 1-Step Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific) was added for 15 minutes at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... This was followed by the plate wash step and an incubation with 1-Step Ultra TMB-ELISA (Thermo Scientific, SG) at 100 μL/well for 3 min ...
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bioRxiv - Biochemistry 2020Quote: ... 5 washes of TBST were done followed by development using 1-Step Ultra TMB-ELISA Substrate Solution (ThermoFisher Scientific, 43028) and quenching with 1N HCl ...
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bioRxiv - Microbiology 2020Quote: ... all peptides at a final concentration of 0.5 μg/mL in 50 mM pH 9.6 Na2CO3/NaHCO3 buffer were coated on ELISA plates (Nunc, Roskilde, Denmark) and incubated overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... a solution of 1% baculovirus particles in 100mM sodium bicarbonate buffer pH 9.6 was absorbed onto the wells of a 384-well ELISA plate (Nunc Maxisorp) using a Tecan Freedom Evo liquid handling robot ...
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bioRxiv - Microbiology 2021Quote: ... Plates were then washed again three times and developed using a SuperSignal™ ELISA Pico Chemiluminescent Substrate (ThermoFisher; Cat#37069) and the level of luminescence detected using a Cytation 3 image reader luminometer using Gen5 software.
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bioRxiv - Immunology 2021Quote: ... Murine IL-2 ELISA capture (catalog no. 14-7022-85) and detection (catalog no. 13-7021-85) antibody reagents were purchased from Invitrogen/eBioscience ...
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bioRxiv - Microbiology 2020Quote: ... washed thrice in PBST and 50 μL of 1-Step Ultra TMB-ELISA was added (Thermo Fisher Scientific, Cat. #34028). Following a 12 to 15-min incubation ...
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bioRxiv - Immunology 2020Quote: ELISAs were performed using 50 ng of VP1 pentamer/well in an EIA/RIA Polystyrene High Bind Microplate (Fisher Scientific) coated overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... gH/gL or gL (diluted in PBS; 100 µl/well) were coated overnight on Nunc MaxiSorp high protein-binding capacity ELISA plates (ThermoFisher). Subsequently ...