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Citations for Thermo Fisher :
2251 - 2300 of 10000+ citations for Mouse IL 17D ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... IL-21 cDNA transcript sequences were amplified by TaqMan probe based qPCR reactions (ThermoFisher, 4331182) using the Applied Biosystems TaqMan Fast Advanced Master Mix (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... Whole-cell protein lysates were prepared in M-PER buffer (Thermo Scientific, Rockford, IL, USA) with 1x Protease & Phosphatase Inhibitor Cocktail (Thermo Scientific ...
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bioRxiv - Microbiology 2024Quote: ... The ligation products (5 μl) were transformed into DH10B Competent Cells (Thermo Scientific, Rockford, IL) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were mounted on gelatin-coated glass slides (SuperFrost Plus, ThermoFisher Scientific, IL, US), rehydrated ...
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bioRxiv - Neuroscience 2023Quote: ... CA) and Supersignal West pico PLUS Chemiluminescent Substrate was purchased from Thermo Scientific (Rockford, IL). Aurum Total RNA Mini Kit ...
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bioRxiv - Cell Biology 2023Quote: ... Poland) and by Western blotting using Pierce High Sensitivity-streptavidin-HRP (Thermo Scientific, Rockford, IL) diluted 1:40,000 in 3% BSA/TBST.
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bioRxiv - Neuroscience 2023Quote: ... pH 7.5) added with a protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific, IL, USA). Protein samples were separated by 10% SDS-polyacrylamide gel electrophoresis (20 µg/lane) ...
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bioRxiv - Microbiology 2023Quote: IL-33 (residues 112–270) and its R169A mutant were cloned into vector pET28a (Invitrogen) and were expressed with a TEV protease cleavable N-terminal His6 tag ...
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bioRxiv - Microbiology 2023Quote: ... Cells were lysed with Pierce Radioimmunoprecipitation (RIPA) buffer (cat. no. 89901, Thermo Scientific, Rockford, IL) with protease and phosphatase inhibitors ...
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bioRxiv - Neuroscience 2023Quote: ... with or without recombinant IL-1β (50ng/ml)(Invitrogen; Thermo Fisher Scientific, Waltham, MA, USA) for 24hr [45 ...
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bioRxiv - Immunology 2023Quote: ... in the presence of 250U Pierce Universal Nuclease for Cell Lysis (ThermoFisher Scientific, Rockford, IL), cells counted on Beckman Vi-Cell XR Cell Counter ...
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bioRxiv - Cell Biology 2023Quote: ... IL) were cultured in Dulbecco’s minimum essential medium (DMEM) high glucose with 10% FBS (Thermofisher) and 100 μg/ml penicillin/streptomycin (Invitrogen ...
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bioRxiv - Genetics 2023Quote: ... and Cas9 (PNA Biosciences Cas9 Protein with NLS, NC1279639, Fisher Scientific, LLC, Chicago, IL, USA) were injected into 240 embryos (240 separate embryos per gRNA ...
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bioRxiv - Immunology 2023Quote: Ectocervical biopsies were fixed for 48 hours in 4% formaldehyde (Thermo Fisher Scientific, Rockford, IL) and paraffin-embedded to generate 5 μm sections used for hematoxylin and eosin (H&E ...
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bioRxiv - Genomics 2023Quote: ... Gels were stained with the GelCode® Blue stain reagent (Thermo Scientific, Rockford, IL, USA) after fixation using 50% methanol (v/v ...
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bioRxiv - Microbiology 2023Quote: ... Nonspecific binding was blocked by Starting Block T20 PBS Blocking Buffer (Thermo Scientific, Rockford, IL) for 1 hour followed by primary antibodies targeting EV membrane protein ...
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bioRxiv - Cell Biology 2024Quote: ... and mounted with a coverslip using ProLong Gold antifade mounting media (Thermo Fisher, Rockford, IL). Images were acquired using a Nikon A1 Confocal microscope (Nikon TE2000 inverted microscope provided by the UAB Microscopy Core).
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bioRxiv - Cancer Biology 2024Quote: ... + 500 U/mL Gibco™ recombinant human (rh) IL-2 (Thermo Fisher Scientific, cat# PHC0021). For primary NK cells ...
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bioRxiv - Bioengineering 2024Quote: ... and 100U/ml PeproGMP™ Human IL-2 Recombinant Protein (Thermo Fisher; 200-02-1MG). The T cell culture was transferred to G-Rex bioreactor vessels (Wilson Wolf ...
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bioRxiv - Biochemistry 2024Quote: Pierce LC-MS grade water and formic acid were purchased from Thermo Scientific (Rockford, IL). LC-MS grade acetonitrile and 2-propanol were purchased from Honeywell (Charlotte ...
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bioRxiv - Neuroscience 2024Quote: ... Bound cytokine was detected using a biotinylated IL-6 detection antibody (ThermoFisher Scientific, MP5-32C11), streptavidin horseradish peroxidase (strep-HRP) ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-ZO-1 (mouse monoclonal, Invitrogen 33-9100, 1:500), rabbit anti-aPKC (rabbit polyclonal ...
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bioRxiv - Developmental Biology 2023Quote: ... Mouse aggregation medium (mouse AM) consisted of 1x DMEM (GIBCO-41965) supplemented with 20% FBS (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-©tubulin (mouse monoclonal, MilliporeSigma T6557, 1:200) and mouse anti-ZO-1 (Life Technologies, 1:300). Rabbit anti-Pard3 (rabbit polyclonal ...
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bioRxiv - Microbiology 2020Quote: ... ELISA plates were blocked using PBS supplemented with 3% bovine serum albumin (PBSA; Thermo Fisher Scientific, Waltham, MA). THL-treated virus was added to blocked KZ52-coated plates and allowed to adsorb at 37°C for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed five times before incubation with 1x TMB ELISA substrate solution (eBioscience; Thermo Fisher Scientific, Inc.) and the reaction was stopped with 1 M H2SO4 ...
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bioRxiv - Neuroscience 2020Quote: ... Human phospho-tau [pT181] was measured using a commercially available ELISA per manufacturer’s instructions (Thermo Fisher Scientific, KHO0631). Guanidine extracted supernatants of hippocampus homogenates described above were utilized for the pTau ELISA.
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bioRxiv - Neuroscience 2020Quote: The level of Aβ in the tissue was assessed by ELISA as per the manufacturer’s instructions (KHB3442, Invitrogen). The resulting measurements were normalized for tissue weight.
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bioRxiv - Microbiology 2022Quote: Headless HA ELISAs were performed using 96-well Immunlon 4HBX flat-bottom microtiter plates (no. 3855; Thermo Fisher) coated with 0.5 ug/well of streptavidin (no ...
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bioRxiv - Neuroscience 2021Quote: ... were prepared for ELISAs by coating each well with 50ul of 1.5ug/ml HT7 monoclonal antibody (Thermo Fisher) the night before use ...
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bioRxiv - Neuroscience 2020Quote: ... whole brain lysates were prepared as for ELISA and protein concentration determined using a BCA assay (Thermo Fisher). Wells were loaded with 30 µg of total protein which was resolved by SDS-PAGE under reducing conditions and transferred onto polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2019Quote: ELISA was performed using anti-GST coated strips (Pierce™ Anti-GST Coated Clear Strip Plates, Thermo Scientific). A fixed amount of 10 pmol GST-tagged PA1217 was diluted in 200 µl PBS (137 mM NaCl ...
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bioRxiv - Neuroscience 2019Quote: ... and results of the ELISA were measured by a colorimetric microplate reader (VarioSkan 2.2; Thermo Scientific Inc., USA) at 450nm ...
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bioRxiv - Pathology 2021Quote: ... After staining with 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific, San Jose, CA, US), mean absorbances in duplicates were read at 450nm and ratio of HAA/PNA was calculated for each sample in order to measure changes of glycosylation ...
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bioRxiv - Immunology 2022Quote: ... and the assay was developed with 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo Fisher, Cat#34029) and blocked with 2N H2S04 solution before reading ...
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bioRxiv - Immunology 2022Quote: ... 200 ng/ml) and medium was collected for analysis of the amount of secreted TNFα by ELISA (Invitrogen).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The concentration of Aß40 and Aß42 in the culture medium was quantified by ELISA (Thermo Scientific, Waltham, MA). Briefly ...
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bioRxiv - Immunology 2022Quote: ... supernatants were collected and cytokines quantified by ELISA according to manufacturers’ instructions (R&D Systems, Biolegend of Invitrogen), except antibody and sample volumes were halved.
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bioRxiv - Neuroscience 2024Quote: ... The concentrations of human α-synuclein were measured using a human α-syn-specific ELISA (ThermoFisher Scientific #KHB0061) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... the plate was developed using 50 µl of 1-StepTM Turbo-TMB-ELISA Substrate Solution (Thermo Fisher Scientific) per well ...
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bioRxiv - Neuroscience 2023Quote: ... the substrate of the peroxidase (100µL, 3,3’,5,5’-tetramentylbenzidine solution; 1-StepTM Ultra TMB-ELISA, 34029, Thermo Scientific) was added in each well for 10 to 25 minutes at RT and in darkness ...
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bioRxiv - Immunology 2023Quote: ELISA plates (Microtitre) were coated with 50 µl/well of 5 µg/ml NeutrAvidin (Thermo Fisher Scientific, #31000) for 1 h at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... 2ug/mL of SARS CoV2 BA.1 RBD protein were coated on 96-well ELISA plates (Nunc Maxisorp) and left overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: Total IgA and IgG ELISAs were performed using 384-well flat-bottom MaxiSorp plates (ThermoFisher, Cat No. 464718) coated with either an unconjugated anti-human IgA (Novusbio ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Concentrations of IL33 and periostin in culture supernatants were measured by ELISA according to the manufacturer’s instructions (Invitrogen, cat # ...
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bioRxiv - Microbiology 2023Quote: ... Plates were developed with 1-Step ultra TMB-ELISA substrate solution (Thermo Fisher Scientific, Waltham, MA, USA, #34028) and incubated at room temperature for 15-30 minutes ...
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bioRxiv - Immunology 2023Quote: ... the ELISA plates were incubated with horseradish peroxidase (HRP)-conjugated goat anti-human IgG antibody (1:5000, Invitrogen) for 1 hour at 37°C ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were then enriched via negative selection using Dynabeads Untouched Mouse CD4 T cells Kit (Thermo Fisher Scientific) according to manufacturer protocol.
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bioRxiv - Immunology 2021Quote: ... mice which were maintained on tamoxifen diet for 4-5 wks and from CD45.1 mice (as described above) which were subjected to T cell depletion using Dynabeads FlowComp Mouse Pan T (CD90.2) Kit (ThermoFisher Scientific). BM cells from WT or FL mice were mixed with the BM of the competitor donor at 1:1 ratio and then transplanted by intravenous injection into lethally irradiated CD45.1 mice (900 rad) ...