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Citations for Thermo Fisher :
2301 - 2350 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Plasmid concentration was measured using Qubit (Thermofisher, catalogue number Q32850). The primers for cloning were ...
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bioRxiv - Cancer Biology 2020Quote: ... Plasmids were transfected into cells using Lipofectamine (ThermoFisher, Waltham, Massachussetts). Plasmids psPAX2 and pMD2.G ...
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bioRxiv - Genetics 2020Quote: ... or Myc-Exosc2-G198D (pAC3465) plasmid using Lipofectamine 2000 (Invitrogen) and cells were collected 48 hr after transfection ...
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bioRxiv - Cancer Biology 2019Quote: Cells were transfected with plasmid vectors using Lipofectamine 2000 (Invitrogen). For stable overexpression of FLAG tagged PITX1 ...
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bioRxiv - Cancer Biology 2019Quote: ... or PureLink HiPure Plasmid Midiprep Kit (Thermo Fisher, Waltham, MA). These were transfected into Tet-On HEK-293TLDcells [10] by incubating 3 µg plasmid DNA with 9 µL Fugene HD (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid transfections were performed with 1.5 μl Lipofectamine 2000 (Invitrogen) per 100 μL Opti-MEM media (Invitrogen) ...
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bioRxiv - Genetics 2020Quote: ... We subcloned this region into the pENTR-1A plasmid (ThermoFisher) using KpnI and XhoI restriction sites on either side of the promoter ...
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bioRxiv - Microbiology 2020Quote: ... Maxipreped plasmids were transiently transfected into 293-F cells (Thermofisher) and the cells were further cultured in suspension for 6 days before harvesting antibody-containing supernatant ...
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bioRxiv - Systems Biology 2020Quote: ... The GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific, Waltham, USA) was used for plasmid isolation ...
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bioRxiv - Microbiology 2020Quote: ... Spike expression plasmids were transfected with Lipofectamine LTX (Life Technologies) following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... Plasmid DNA concentration was measured by fluorometric quantification (Qubit, ThermoFisher) and copy number was normalized between samples in the same experimental condition according to the M.W ...
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bioRxiv - Microbiology 2020Quote: The plasmid pMR-RQ-Bb/SA11g72AFLUnaG was obtained from ThermoFisher and contained a modified synthetic cDNA of SA11 segment 7 (NSP3) ...
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bioRxiv - Biochemistry 2020Quote: ... 1 μg plasmid DNA was added with Lipofectamine 3000 (Invitrogen), 50 μl/ml OPTI-MEM (Gibco)/DMEM ...
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bioRxiv - Genomics 2021Quote: ... Plasmids and 60 µl Lipofectamine 2000 reagent (Thermo Fisher Scientific) were each diluted in 1 ml of OptiMEM ...
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bioRxiv - Immunology 2021Quote: Plasmids were transfected into Expi293F cells (ThermoFisher Scientific, Waltham, MA) in a 1:1 ratio of heavy chain:light chain using Expifectamine according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... and isolated using a GeneJET plasmid miniprep kit (ThermoFisher, K0503). Sequencing was performed by MCLAB (Molecular Cloning Laboratories).
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bioRxiv - Cell Biology 2019Quote: ... The control GFP-NLS plasmid was from Invitrogen (V821-20). To knock down expression of RTN4 ...
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bioRxiv - Cell Biology 2019Quote: ... Plasmid mistures were diluted in 3 ml OptiMEM (Life Technologies) and supplemented with 60 μl Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Genetics 2021Quote: ... Parallel transfection with an eGFP expression plasmid (240 ng; Invitrogen) was used to determine nucleofection frequencies (>90%) ...
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bioRxiv - Genetics 2020Quote: The TyrgRNA-Tomato plasmid was purchased from GenScript and prepared using PureLink™ HiPure Plasmid Midiprep Kit (Invitrogen). Plasmid (10 ng/µL) ...
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bioRxiv - Cell Biology 2021Quote: ... miniprepped DNAs using PureLink™ Quick Plasmid Miniprep Kit (Invitrogen) were analyzed by Sanger sequencing.
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bioRxiv - Cell Biology 2021Quote: ... Plasmid transfection was performed using Lipofectamine reagent (Thermo Fisher Scientific) according to the manufacturer’s protocol.
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bioRxiv - Physiology 2021Quote: ... the plasmid was transfected using Lipofectamine 2000 (Thermo Fisher Scientific) into BHK-21 cells grown in GMEM with 2% FBS and 10% TPB at 37°C with 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... PCR product was mixed with the donor plasmid pDONR207 (Invitrogen), and subjected to a recombination reaction with Invitrogen Gateway BP Clonase™ ...
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bioRxiv - Cell Biology 2021Quote: ... PShuttle-CMV plasmids were then digested overnight with MssI (ThermoFisher) and linearized pShuttle-CMV plasmids were transformed into the final viral backbone using electrocompetent AdEasier-1 cells ...
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bioRxiv - Cell Biology 2020Quote: ... coli using a PureLink HiPure Plasmid Miniprep Kit (Thermo Fisher) and used to transfect Sf9 cells using Cellfectin II transfection reagent (Thermo Fisher) ...
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bioRxiv - Biochemistry 2021Quote: ... and GeneJET Plasmid Miniprep Kit were bought from Thermo Fisher Scientific.
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bioRxiv - Cell Biology 2021Quote: ... Plasmid DNA was transformed into OneShot Top10 competent cells (Invitrogen). Oligonucleotides were synthesized by Integrated DNA Technologies.
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bioRxiv - Synthetic Biology 2021Quote: ... The pT7-CFE-GFP plasmid was purchased from Thermo Fisher Scientific and prepared at different concentrations.
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bioRxiv - Neuroscience 2021Quote: Plasmid and Si-RNAs were transfected with Lipofectamine 2000 (Invitrogen) and RNAi max (Invitrogen ...
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bioRxiv - Genetics 2020Quote: ... subcloned into the pCR™2.1-TOPO® plasmid (Thermofisher), and 5–7 clones were sequenced in both directions by Sanger sequencing at Retrogen® and/or Genewiz® to identify both alleles in a each fly ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmids were isolated using a mini prep kit (Thermo Fisher). DNA concentration was measured on a NanoDrop (Thermo Fisher ...
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bioRxiv - Biophysics 2020Quote: ... Cells were transfected with plasmids using Lipofectamine 3000 (Life Technologies) according to the manufacturer’s protocol.
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bioRxiv - Bioengineering 2021Quote: ... and 50 ng of attD plasmid using Lipofectamine 2000 (Invitrogen). 2-3 days after transfection of cells with all three plasmids ...
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bioRxiv - Microbiology 2021Quote: ... remove plasmid DNA template by treating with Turbo DNAse (Ambion), subsequently subject to phenol-chloroform ...
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bioRxiv - Microbiology 2021Quote: ... plasmids were linearized by digestion with AatII (FD0994, ThermoFisher Scientific) and in vitro transcribed using the MegaScriptTM T7 Transcription Kit (AMB1334 ...
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bioRxiv - Immunology 2021Quote: ... plasmids were transfected for 24h using Lipofectamine 3000 (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... coli clones using a GeneJET plasmid miniprep kit (Thermo Fisher), and DNA was sequenced using a Sanger sequencer ...
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bioRxiv - Cancer Biology 2021Quote: ... and the expression plasmid were combined in Opti-MEM (Invitrogen). Trans-IT transfection reagent (Mirus ...
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bioRxiv - Cell Biology 2020Quote: ... Human RAB34 cDNA was obtained from GE Healthcare (MHS6278-202807876) and cloned into Gateway pENTR plasmid pDONR221 (Invitrogen); RAB34 mutants were prepared by site-directed mutagenesis of pENTR-Rab34 ...
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bioRxiv - Cell Biology 2021Quote: All constructs were sub-cloned into pcDNA 3.1 plasmid (Invitrogen). The plasmids pcDNA3.1-F36M-FKBP(Fm)-emGFP-hFt and pcDNA3.1–Fm–hFt were previously described32 ...
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bioRxiv - Genetics 2020Quote: Plasmid constructs were generated performing Multi-Site Gateway cloning (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Plasmids were constructed using two-slot Gateway Cloning system (Invitrogen) and confirmed by restriction digest and/or sequencing as appropriate ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1.5 µg target plasmid DNA using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Assembled plasmids were transformed into Mach1 competent cells (Invitrogen #C862003) for amplification and purified with miniprep (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: Plasmid transfections were performed using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Each synonymous plasmid mixed with turbofect reagent (12331863, Fisher scientific) was added in each well and cells were sampled at day 2 (Trypsin-EDTA ...
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bioRxiv - Cell Biology 2022Quote: ... The plasmids were digested with EcoRI (ThermoFisher, Scientific, Waltham, MA) and RNA was in vitro transcribed using mMESSAGE mMACHINE Sp6 Transcription Kit ...
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bioRxiv - Cell Biology 2022Quote: Plasmid transfections were performed using Lipofectamine 2000 (Thermo Fisher Scientific) and siRNA transfections were performed using Lipofectamine RNAiMAX (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... For further processing the GeneJET Plasmid Miniprep Kit (Thermo Fisher) was utilized as instructed ...