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Citations for Thermo Fisher :
2151 - 2200 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Luminex assay was performed using ProcartaPlex Human Basic Kit (Invitrogen) and a panel of ProcartaPlex Human Simplex (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... and 20 ng/ml human bFGF (PHG0021, Thermo Fisher Scientific)) ...
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bioRxiv - Synthetic Biology 2024Quote: Rabbit polyclonal anti-Human ASGR1 antibody (Thermo Fisher, PA5-80356), 1:1,000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant Human EGF (Invitrogen, Cat#PHG0313, 100 µg/500 mL), Hydrocortisone (Sigma ...
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bioRxiv - Neuroscience 2024Quote: Gibco Human Astrocytes (cat. nr. K1884, Lot nr. 802268, Invitrogen) were sourced from a 20 weeks old male donor ...
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bioRxiv - Immunology 2024Quote: ... goat anti human IgG (H+L) HRP (Invitrogen, Cat#31412) was added to wells at a 1:10,000 dilution for one hour at room temperature in the dark ...
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bioRxiv - Neuroscience 2024Quote: ... or a human NT3 ELISA kit (Thermo Fisher Scientific, EHNTF3). Both ELISAs were performed as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... then washed twice with human wash buffer (DMEM/F12 (Gibco), 10 mM HEPES ...
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bioRxiv - Cancer Biology 2024Quote: ... human neutrophils were labeled with calcein AM (Thermo Fisher Scientific) and left to migrate for 2 hours before termination of the experiment ...
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bioRxiv - Bioengineering 2024Quote: ... Research-grade DynabeadsTM Human T-Expander CD3/CD28 beads (Gibco) were washed using DynaMag-2 magnet (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... CAFs were kept in Human Plasma-Like Medium (HPLM, Gibco) with ascorbic acid for 11 days ...
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bioRxiv - Cancer Biology 2024Quote: ... and 0.2 Units/mL human recombinant insulin (Gibco, #12585-014), seeded on plates coated with rat tail collagen (BD Biosciences) ...
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bioRxiv - Immunology 2024Quote: ... Dynabeads Human T-Activator CD3/CD28 (11161D; Gibco; 1:100) or plate bound with anti-CD3 and anti-CD28 antibodies (0.5µg/ml each ...
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bioRxiv - Immunology 2024Quote: ... or anti-human IgG Alexa Fluor 647 conjugate (Invitrogen, A21445) or Streptavidin Alexa Fluor 647 conjugate (Invitrogen ...
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bioRxiv - Physiology 2024Quote: Human islets were cultured in CMRL medium (Gibco 11530-037) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... and 5 ng/mL recombinant human EGF (Gibco Cat# PHG0311).(22 ...
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bioRxiv - Cell Biology 2024Quote: ... siRNAs were purchased from Life Technologies (for human cell lines) and from Horizon Discovery (for human PCLS) ...
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bioRxiv - Cancer Biology 2024Quote: ... recombinant human EGF (Thermo Fisher Scientific, #PHG0311, 50 ng/ml), N-acetylcysteine (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2020Quote: ... The following day cells were co-transfected with 2 µg of Fluc plasmid DNA and 20 ng of Rluc control plasmid (pRL) per well using Lipofectamine 2000 (Thermo Fisher, 116688-019) and incubated for 16 h at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... HEK293T/17 cells at sub-confluence in 6-well plates or 100 mm dishes were transfected with HLA-DRA plasmid or HLA-DRB1 plasmid or a 1:1 combination of both using the Lipofectamine 3000 transfection reagent (Thermo Fisher Scientific, UK) according to manufacturer instructions ...
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bioRxiv - Biochemistry 2019Quote: ... lentivector plasmids were transfected into HEK293T cells together with packaging and envelope plasmids (psPAX2 and pMD2.G) using Lipofectamine 3000 (Thermo Fisher Scientific, L3000008). Three days after transfection ...
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bioRxiv - Cell Biology 2021Quote: The human MISP sequence harbored in a pCMV-SPORT plasmid (Harvard PlasmID Database; HsCD00326629) was subcloned by PCR and TOPOcloned into a pCR™8 Gateway entry vector (Invitrogen; 46-0899). In-frame sequence insertion was confirmed by sequencing ...
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bioRxiv - Cell Biology 2021Quote: ... For lentivirus particle production these plasmids were recombined with pCDH-EF1a-Gateway-IRES-BLAST plasmids as described in [51] using LR Clonase II (Invitrogen/Thermo Fisher Scientific).
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bioRxiv - Genomics 2022Quote: ... LINC000881 overexpression was achieved by lipofectamine based transfection of LINC00881 plasmid versus bacterial alkaline phosphatase control plasmid using RNAiMAX reagent (ThermoFisher Scientific, Waltham, MA). iPSCs were harvested at 48-72 hours following transfection for functional and gene expression studies ...
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bioRxiv - Microbiology 2021Quote: ... jejuni PglB was amplified by PCR from the plasmid pACYCpgl with the pTac promoter and LacIq repressor from plasmid pEXT20 as a template using Pfx Polymerase (Thermo Fisher Scientific UK) with (SEQ ID 15 ...
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bioRxiv - Immunology 2022Quote: ... The cells were co-transfected with 650 μg of SOSIP expressing plasmid DNA and 150 μg of furin expressing plasmid DNA complexed with 293Fectin (ThermoFisher Cat No. 12347019). On day 6 cell culture supernatants were harvested by centrifugation of the cell culture for 30 min at 3500 rpm ...
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bioRxiv - Molecular Biology 2019Quote: To generate linear plasmids without telomeric ends, the circular plasmids were amplified with primers (BP1204, BP1205; Table 2) using Phusion polymerase (Thermo Fisher Scientific, USA). Cycling conditions were ...
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bioRxiv - Synthetic Biology 2022Quote: ... we developed a vacuum-based plasmid extraction protocol named “TeVacS” on Fluent using the PureLink™ Pro Quick96 plasmid purification Kit (K211004A) (Invitrogen, Carlsbad, CA). Our customized protocol features the use of vacuum manifold for efficient removal of washing buffer and final elution of plasmid DNA into a 96-well plate placed on a 3D-printed holder ...
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bioRxiv - Developmental Biology 2022Quote: ... 90 ng of ASD library plasmids and 30 ng of control library plasmids were used for electroporation of MegaX DH10B T1R Electrocomp™ Cells (Thermofisher, cat.no. C640003) following the manufacturer’s guide ...
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bioRxiv - Physiology 2022Quote: ... HPAECs were transfected with either control plasmids (empty vector) or 1μg of HA-ubiquitin plasmids (HA-tagged Ubi) using Lipofectamine 3000 (Thermo fisher Scientific, PA, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... a TMPRSS2 plasmid and S plasmids from SARS-CoV-2 variants into 293T cells using Lipofectamine 3000 transfection reagent (L3000-001, ThermoFisher Scientific, Asheville, NC) 39,40 ...
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bioRxiv - Microbiology 2022Quote: ... we isolated fresh plasmid DNA for use in transfection experiments by using the PureLinkTM HiPure Plasmid Maxiprep Kit (Invitrogen, Thermo Fisher Scientific Corp.). This method minimizes lipopolysaccharide contamination in plasmid DNA extracted from Escherichia coli and reduces non-specific PRR pathway activation in plasmid DNA transfections of mammalian cells ...
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bioRxiv - Microbiology 2022Quote: ... we isolated fresh plasmid DNA for use in transfection experiments by using the PureLinkTM HiPure Plasmid Maxiprep Kit (Invitrogen, Thermo Fisher Scientific Corp.). This method minimizes lipopolysaccharide contamination in plasmid DNA extracted from Escherichia coli and reduces non-specific PRR pathway activation in plasmid DNA transfections of mammalian cells ...
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bioRxiv - Genetics 2024Quote: ... A subset of the saturated culture was used to extract purified plasmid DNA using a GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific, K0503).
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bioRxiv - Biophysics 2022Quote: ... 2 μg total plasmid DNA (725 ng CagFbFP-Q148V plasmid + 1275 mg MTS-CagFbFP plasmid) and 2 μl PLUS Reagent were diluted in 100 μl Opti-MEM (Thermo Fisher Scientific, USA), and 4 μl Lipofectamine LTX were diluted in 100 μl Opti-MEM ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293T cells were transfected by transfer plasmid and packaging plasmids psPAX2 (addgene #12260) and pMD2.G (addgene #12259) using Lipofectamine 3000 reagent (Thermo Fisher Scientific # L3000015). Viral particles were concentrated using Lenti-X Concentrator (Takara Bio ...
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bioRxiv - Biochemistry 2023Quote: ... Both pDEST-CMV-N-EGFP-wtG3BP2 and pDEST-CMV-N-EGFP-C73SG3BP2 plasmids were amplified and extracted using PureLink™ HiPure Plasmid Maxiprep Kit (Invitrogen™, K210007) before use.
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bioRxiv - Molecular Biology 2024Quote: ... HEK 293T cells were transiently co-transfected with individual expression plasmids encoding full-length RAI2 and CtBP1 and the reporter plasmid using Lipofectamine 2000 (ThermoFisher Scientific, MA, USA). Co-transfection with the pEGFP-N2 plasmid (Takara Bio ...
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bioRxiv - Neuroscience 2024Quote: ... prior to the expansion of resistant colonies in 100 mL of Luria-Bertani (LB) broth and purification of plasmid DNA using a PureLink HiPure Plasmid Filter Midiprep Kit (Thermo Fisher Scientific; #K210014), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Cas9 over expressing cells were transduced with lentiviruses expressing sgRNA targeting human hypoxanthine phosphoribosyltransferase 1 (HPRT1) (LentiArray™ CRISPR Positive Control Lentivirus, human HPRT, Thermo Fisher Scientific Cat # A32829). HPRT1 knockout cells shows resistance to 6-thioguanine (6TG ...
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bioRxiv - Immunology 2022Quote: ... The supernatants of human CD19 CAR T cells or human TCR transfected CD8+ T cells were analyzed by IFNγ Human Uncoated Elisa Kit (Invitrogen, Thermo Fisher Scientific # 88-7316-88). Plates were read on BioRad plate reader at a wavelength 450 nm and subtracted by 595 nm.
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bioRxiv - Molecular Biology 2022Quote: Female human embryonic kidney cells HEK-293FT and male human fibrosarcoma HT1080 cells were cultured in DMEM-GlutaMAX + Pyruvate (Life Technologies SAS, Saint-Aubin, France), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Molecular Biology 2019Quote: ... was performed using the TaqMan gene expression assays (EFHA1/Micu2 rat: s235193; EFHA1/Micu2 human: s47976; Hprt1 rat: Rn01527840; Hprt1 human: Hs99999909, Applied Biosystems, Life Technologies, Carlsbad, CA), using a 7900HT Fast Real-Time System (Applied Biosystems) ...
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bioRxiv - Immunology 2024Quote: ... the blots were probed for the presence of heavy and light chain bands using either horseradish peroxidase (HRP)-conjugated polyclonal goat anti-human IgG Fc antibody or polyclonal goat anti-human kappa chain antibody (Thermo Fisher Scientific, Waltham, MA, USA). Antibody-coupled HRP activity was detected with Supersignal West Dura Extended Duration Substrate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: Human promyelocytic leukemia (HL-60) cells and human umbilical vein endothelial cells (HUVECs) were grown in RPMI-1640 medium (Gibco, Thermo Scientific, MA, United States) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: ... or pMK-RQ (EtIMP1Cit) plasmid with appropriate restriction enzyme sites for cloning into pYD1 yeast display plasmid vector (Invitrogen, Thermofisher Scientific, Waltham, MA, USA). A NotI restriction site was included between each antigen sequence and the 3’citrine tag ...
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bioRxiv - Immunology 2020Quote: ... 1 × 105 cells were transfected with the appropriate plasmid combinations or plasmid/siRNA combinations using the Neon Transfection System (Thermo Fisher, Waltham, MA, USA) and a 10 µL Neon tip at 1000 V ...
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bioRxiv - Immunology 2022Quote: ... 12μg heavy chain plasmid and 12 μg of light chain plasmid were added into 3ml of Opti-MEM™ (Thermo Fisher Scientific cat.# 31985070), after inverting ...
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bioRxiv - Cancer Biology 2024Quote: ... Retrovirus was generated by transfecting oncogenic plasmids and viral packaging plasmid (pCL-Ampho) into phoenix cells with Lipofectamine 2000 (Life Technologies, Grand Island, NY). Viral particles were incubated with prostate cells (70% confluence ...
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bioRxiv - Immunology 2023Quote: ... Sequence-verified Cas9-gRNA-Puro plasmids and pMA donor plasmid were co-transfected into in-house C57BL6/N 6.0 ESC using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA) prior to selection with 2 μg/ml puromycin for 48 hours ...