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Citations for Thermo Fisher :
2251 - 2300 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: The backbone plasmid pcDNA6/V5-His was purchased from Invitrogen Inc ...
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bioRxiv - Microbiology 2021Quote: ... plasmid DNA was extracted using HiPure Maxiprep kit (Life TECHNOLOGIES).
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bioRxiv - Cell Biology 2020Quote: ... siRNA and plasmid transfections were performed using Lipofectamine RNAiMAX (Invitrogen) and Effectene (QIAGEN) ...
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bioRxiv - Neuroscience 2020Quote: ... The plasmids were transfected with Lipofectamine 2000 (11668019, Thermo Fisher) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Mammalian expression plasmids were generated using the GatewayTM technology (Invitrogen). Entry clones pDNR-UL44(2-433) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were transfected with the plasmids using Lipofectamine 3000 (Thermofisher) at standard concentrations as suggested in the manufacturer protocols ...
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bioRxiv - Cell Biology 2021Quote: Plasmids were transfection into HEK293Tcells with lipofectamine 2000 reagent (Invitrogen) according to manufacturer’s instruction ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were propagated in Stbl3 chemically competent E.coli (ThermoFisher C737303). Plasmid sequences were verified by Sanger sequencing (GENEWIZ Inc).
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bioRxiv - Cancer Biology 2021Quote: All expression plasmids were generated with the Gateway system (Invitrogen). The Entry vector pDONR223-MAGI1 was a gift from William Hahn (Addgene plasmid #23523 ...
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bioRxiv - Molecular Biology 2022Quote: ... This plasmid was transformed into Escherichia coli TOP10 cells (Invitrogen). Cells were grown at 37 °C to log phase at an OD600 of 1.7-1.9 in Terrific-Broth (TB) ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmid transfections were performed with lipofectamine 3000 (Thermo Fisher Scientific) in HEK293 SPTLC1-KO cells ...
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bioRxiv - Cancer Biology 2022Quote: ... isolated with the Gene JET Plasmid Miniprep Kit (Thermo Scientific) and approved by Sanger sequencing (Starseq) ...
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bioRxiv - Molecular Biology 2022Quote: Plasmid constructs were PEI or Lipofectamine 3000 (Invitrogen, L3000-008) transiently transfected into HEK293T cells for 12-16h in 96 ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μg GFP plasmid and 2 μl Lipofectamine 2000 (Thermofisher) with DNA:reagent ratio 1:2 were added into 25 μl of DMEM media in separate with DNA:reagent ratio 1:2 were added into 25 μl of DMEM media in separatetubes and incubated for 5 min at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids were complexed with PEI in Opti-MEM medium (Thermofisher) at a 1:3 ratio ...
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bioRxiv - Developmental Biology 2022Quote: ... Ligated plasmids were transformed into DH5α cells (FEREC0111, Fisher Scientific). Colonies were picked and sequenced via Sanger sequencing by Eurofins Genomics to verify insert ...
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bioRxiv - Genetics 2022Quote: ... these were transfected with 0.5pmol plasmid using Lipofectamine 3000 (Invitrogen). 48h after transfection ...
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bioRxiv - Microbiology 2022Quote: HeLa cells were transfected with plasmid using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2022Quote: ... Plasmids were transfected using Lipofectamine 3000 Reagent (Thermo-Fisher Scientific). Luciferase activities were measured and plotted relative to lysate protein concentrations using the Promega Dual Luciferase Reporter (Promega ...
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bioRxiv - Cancer Biology 2022Quote: ... Plasmid and siRNA transfections were performed using Lipofectamine 3000 (Invitrogen) or FuGENEHD transfection Reagent (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... All plasmid constructs were verified by sequencing (BigDye; Applied Biosystems) and/or restriction digestion.
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bioRxiv - Immunology 2022Quote: ... both plasmids were diluted in OptiPROTM SFM (ThermoFisher Scientific 12309050). Transfected cells were incubated at 37 °C with 5% CO2 on a Digital Orbital Shaker (Southwest Science SBT300 ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmid transfection was performed by Lipofectamine LTX (Thermo Fisher Scientific) as described in the manufacturer’s manual ...
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bioRxiv - Neuroscience 2021Quote: ... Transfections of plasmid constructs were performed using Lipofectamine 2000 (Invitrogen) for HEK293T and Lipofectamine LTX (Invitrogen ...
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bioRxiv - Plant Biology 2021Quote: Plasmids were constructed using Multisite Gateway cloning (Thermo Fisher Scientific). The list of primers used for cloning are presented in Table S1 ...
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bioRxiv - Cell Biology 2021Quote: ... from GENEWIZ or luciferase plasmid (pCMV-Luc) from Invitrogen (50 μg each) was then mixed with the microbubbles (5×105 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The resulting plasmids were screened by SacI (Thermo Scientific, ER1131) restriction digest and further Sanger sequenced to check for mutations ...
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bioRxiv - Cell Biology 2021Quote: ... For simultaneous transfection of siRNA and plasmid DNA Lipofectamin2000 (Invitrogen) transfection reagent was applied according to manufacturer protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... and the XhoI sites of pCMV-SPORT6-hACVR1 plasmid (Invitrogen). The R26NALK2 knock-in plasmid ...
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bioRxiv - Neuroscience 2021Quote: ... The plasmid was made using the Gateway system (ThermoFisher, 12538120) with combinations of entry and destination plasmids as follows ...
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bioRxiv - Microbiology 2020Quote: ... plasmid p5522 using the Gateway cloning technology (m Fisher Scientific) (Fig ...
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bioRxiv - Neuroscience 2020Quote: ... Transfections of plasmid DNA were performed using Lipofectamine 2000 (Invitrogen) according to manufacturer’s instructions or for HEK293 cells by polyethylenimine (PEI ...
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bioRxiv - Neuroscience 2021Quote: ... Plasmids were transfected into HeLa cells using Lipofectamine 2000 (Invitrogen) at a ratio of donor plasmid to acceptor plasmid of 1:3 for ITRACK and 1:2 for IDOCKS ...
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bioRxiv - Neuroscience 2021Quote: ... neurons were transfected with plasmids using Lipofectamine 2000 (Thermo Fisher) according to manufacturer’s instructions at days in vitro (DIV ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid DNA was grown in Top10 chemical component cells (ThermoFisher), digested with the corresponding enzymes ...
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bioRxiv - Cancer Biology 2021Quote: ... was cloned into plasmid pcDNA5/FRT/TO (Thermo Fisher Scientific) bearing an mCherry and mNeon tandem fluorescent tag (tFT ...
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bioRxiv - Microbiology 2021Quote: ... and was subcloned in the Thymidine kinase Cypridina plasmid (Thermofisher) in-frame with the Cypridina luciferase gene ...
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bioRxiv - Neuroscience 2020Quote: ... Plasmids encoding MARK4 was transfected with Lipofectamine 2000 (Thermo Fisher) according to the manufacturer’s protocol.
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bioRxiv - Bioengineering 2020Quote: ... The plasmid was extracted with GeneJET Extraction Kit (Fisher Scientific). The identity of the clones was confirmed by sequencing at Macrogen.
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bioRxiv - Bioengineering 2020Quote: ... The plasmid was extracted with GeneJET Extraction Kit (Fisher Scientific), and sequenced at Macrogen for confirmation of the mutation.
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bioRxiv - Developmental Biology 2022Quote: ... we created recombinant pCRTM4-TopoTM plasmids (Invitrogen, Carlsbad, CA, USA) containing 18S and 28S ribosome RNA PCR amplicons ...
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bioRxiv - Microbiology 2022Quote: ... For subcloning of plasmid pET302/ply006 purified pET302 vector (Invitrogen) and codon optimized synthetic DNA (Gene Art DNA Strings ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Humanized anCas were cloned into pcDNA3.1 plasmid expression vector (ThermoFisher). The sgRNA target sequences were designed with Breaking-Cas web tool62 and cloned into MLM3636 plasmid vector (Addgene #43860 ...
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bioRxiv - Immunology 2022Quote: ... The plasmid was transiently transfected into freeStyle293F cells (Thermo Fisher). Protein was purified from filtered cell culture supernatant by Ni-NTA affinity column first and then by a StrepTactin (IBA ...
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bioRxiv - Cell Biology 2022Quote: ... The resulting plasmids were transiently transfected into HEK293F cells (ThermoFisher). Cells were maintained in FreeStyle 293 medium and transfected using the PEI Max reagent (Polysciences Inc. ...
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bioRxiv - Cell Biology 2022Quote: ... EXOC6-HALO and EXOC7-HALO plasmids (Lipofectamine 2000; 11668019; Invitrogen) according to manufacturers’ instructions ...
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bioRxiv - Biophysics 2019Quote: ... The Wizard SV Gel and PCR Clean-Up System were purchased from Promega while the GeneJet Plasmid Midiprep Kit was purchased from ThermoFisher Scientific (Waltham ...
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bioRxiv - Plant Biology 2019Quote: ... Plasmids were then extracted following manufacturer’s instructions from Thermo Scientific GeneJET Plasmid Miniprep Kit ...
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bioRxiv - Neuroscience 2019Quote: All plasmids were made using the Gateway system (ThermoFisher, 12538120) with combinations of entry and destination plasmids as follows ...
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bioRxiv - Cancer Biology 2019Quote: ... The plasmids were propagated in DH5α bacterial cells (Life Technologies). The constructs were verified by sequencing ...