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Citations for Thermo Fisher :
2301 - 2350 of 10000+ citations for 6 Benzothiazolamine 2 1 methylpropyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... HEK293T cells were plated in 5mL DMEM supplemented with 10% FBS and 1% PSG in one well of a 6-well plate (Fisher Scientific) for each validation ...
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bioRxiv - Neuroscience 2023Quote: ... 500,000 cells were plated in 6-well dishes and 1 μg of DNA was transfected when cells reached 75-85% confluency with Lipofectamine 2000 (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... PBS was replaced with a 1:6 (weight: volume) solution of 0.5 M acetic acid (984303; Thermo Fisher, Waltham, MA, USA) with 1 mg/ml pepsin (20343 ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by 40 cycles of 95°C for 15 s and 60°C for 1 min (Applied Biosystems QuantStudio 6 Flex). For testing of U7 snRNA level in U7 KD cells ...
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bioRxiv - Biochemistry 2023Quote: ... 0.1 μM 6-JOE-conjugated reverse primer (5’-6-JOE-GATGATCTCCACCTTGCCGT-3’) was extended with 1 pmol of RNA as template using Superscript III (Thermo Fisher) as reverse transcriptase ...
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bioRxiv - Immunology 2023Quote: ... and 1 × 10^6 were processed using Click-iT EdU Pacific Blue Flow Cytometry Assay Kit (Thermo Fisher Scientific Cat. #C10418). The other 1 × 10^6 were subjected to anti-CD3 crosslinking and pERK staining as described below ...
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bioRxiv - Biochemistry 2023Quote: ... Bacmid DNA containing the construct of interest was transfected into 2mL of Sf9 cells at a density of 1×106 cells/mL on a 6-well plate using ExpiFectamine Sf Transfection Reagent (Life Technologies) and following the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... Cultures were passaged every 5-6 days as small clumps by dissociation with a buffer containing 1 mg per ml Collagenase IV (ThermoFisher Scientific), 0.025% Trypsin (ThermoFisher Scientific) ...
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bioRxiv - Bioengineering 2023Quote: ... Sigma-Aldrich 7722-84-1) and then slowly injecting 0.03 % w/v sodium dithionite (ACROS Organics 7775-14-6, in PBS) using a syringe pump (10 µL min−1 ...
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bioRxiv - Biophysics 2023Quote: ... 400 μg of calmodulin and myosin-5 expression vectors (at a 1:6 ratio) were mixed with 15 ml FreeStyle 293 media (Thermo Fisher) and 1.2 ml of PEI (Polysciences ...
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bioRxiv - Neuroscience 2023Quote: ... into low passage HEK-293T cells plated at 1×106 cells/well of a poly-D-lysine-coated 6-well plate using Lipofectamine 3000 (Life Technologies). The next day ...
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bioRxiv - Cell Biology 2023Quote: ... cell pellets corresponding to two wells of a 6-well plate (double-well pellet with same treatment conditions) were lysed in 1% (v/v) TritonX-100 buffer (Fisher Scientific) in DPBS supplemented with 1% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: Tumors were engrafted by inoculating 1×106 Nalm-6 cells expressing firefly luciferase and GFP intravenously in 100 μL of PBS (Gibco, USA). Mice were randomly assigned to 2 experimental groups and 1 control group ...
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bioRxiv - Biochemistry 2024Quote: ... wash buffers were removed and replaced with a ∼7.6 pH nigericin buffer standard (25 mM HEPES, 80 mM KCl, 1 mM MgCl2) supplemented with 10 μM nigericin (Invitrogen, N1495). Cells were incubated at 37°C for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... Intact spheres were subsequently diluted 1:10 in PBS and placed in a 6 well plate (Fisher Scientific, #07-000-646). Using a standard tissue culture microscope and a 20 uL pipet tip ...
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bioRxiv - Biochemistry 2024Quote: IL-6 secretion from THP-1-derived macrophages was quantified in the growth media using enzyme-linked immunosorbent assay (ELISA; Invitrogen, #BMS213INST) following the manufacturer’s protocol.
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bioRxiv - Biochemistry 2024Quote: ... The wells were then washed 6 times with wash buffer and subsequently incubated with 100 µL per well of 1:10,000 streptavidin-HRP (Thermo Scientific 21130) in dilution buffer for 1 hr at room temperature ...
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bioRxiv - Systems Biology 2024Quote: ... Purified CD14+ monocytes were seeded at a density of 1 million cells/well in 6-well plates in RPMI 1640 (Life technologies) with 10% serum and 20μg/ml recombinant human MCSF (Peprotech ...
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bioRxiv - Molecular Biology 2020Quote: ... containing 2 μM Fura-2 acetoxymethyl ester (Fura-2 AM; ThermoFisher Scientific) and 0.01% pluronic acid (Merck ...
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bioRxiv - Genetics 2023Quote: ... neuronal rosetes were passaged as small clusters in either a 1:1 or 1:2 ratio using the StemPro™ EZPassage™ Disposable Stem Cell Passaging Tool (Gibco, #23181010) or via hand-picking ...
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bioRxiv - Bioengineering 2023Quote: ... then incubated at room temperature in the dark for 2 h with secondary antibodies (AlexaFluor 488 goat anti-mouse IgG 1:400 or 1:600) or rhodamine phalloidin (Invitrogen, R415, 1:600) to visualize F-actin ...
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bioRxiv - Synthetic Biology 2021Quote: ... Isolated B cells were activated at 1 x 106 cells mL-1 for 2 days in IMDM medium (ThermoFisher) with 10% fetal bovine serum ...
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bioRxiv - Genomics 2020Quote: ... 0.5-1×106 proliferating TILs were seeded with 100×106 normal donor peripheral blood mononuclear cells irradiated with 5,000 rad in a 1:1 mixture of TIL media and AIM-2 media (ThermoFisher), supplemented with 30 ng/mL OKT3 (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2019Quote: ... The fixed cells were stained with 1 µM 2’-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]-2,5’-bi-1H-benzimidazole trihydrochloride trihydrate (Hoechst 33342, Invitrogen) for 5 minutes ...
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bioRxiv - Genomics 2021Quote: ... After 2 days of transduction hNGN2-eGFP media was replaced with 1:1 ratio of Neurobasal A (ThermoFisher, 10888022) and DMEM/F12 medium containing GlutaMAX ...
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bioRxiv - Biochemistry 2020Quote: ... air-drying overnight and incubating with 2 μM TOTO-1 iodide (1 mM solution in DMSO, Thermo Fisher Scientific) for 15 min.
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bioRxiv - Microbiology 2021Quote: ... Previously pelleted spheroplasts were resuspended in 1 mL 10mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer solution (Gibco) and 100 μl were collected as whole spheroplasts ...
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bioRxiv - Microbiology 2020Quote: ... The monolayers were then overlaid with 1 to 2 ml of a mixture of 1 part 0.6% agarose (Invitrogen) and 1 part complete Eagle Minimal Essential Medium (VWR ...
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bioRxiv - Plant Biology 2019Quote: ... Rh (10 µg L-1) and Ir (5 µg L-1) in 2% trace analysis grade (Fisher Scientific, UK) HNO3 ...
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bioRxiv - Microbiology 2021Quote: ... 800 µl of isolation medium (DMEM, supplemented with 2% FBS, 1% penicillin-streptomycin and 1% amphotericin B, ThermoFisher Scientific) was added to each well ...
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bioRxiv - Physiology 2020Quote: ... the medium was switched to DMEM/F12 (1:1) plus 2% New Zealand fetal bovine serum (Thermo Fisher Scientific), hormones ...
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bioRxiv - Immunology 2021Quote: ... at 1:0.5:2 molar ratio using Lipofectamine™ 2000 (0.6 ul per 1 ug DNA; Invitrogen™, 11668019); growth medium was refreshed 16 h after transfection ...
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kinesin recruitment by adapter SKIP on melanosomes is dynamically controlled by LC3B phosphorylationbioRxiv - Cell Biology 2021Quote: ... The tumor was excised and was washed in homogenization buffer (0.25 M sucrose [Merck, CAS 57–50–1], 10 mM HEPES, 1 mM EDTA, 2% antibiotic and antimycotic [ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... for 1 h at room temperature and stained for 10 min with 4,6-diamidino-2-phenylindole (DAPI; 1 mg/ml stock solution diluted 1:50,000 in PBS; Thermo Fisher) at room temperature with at least four PBS washes in-between ...
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bioRxiv - Immunology 2022Quote: ... resuspended in 1 mL nuclei wash buffer (2% BSA and 0.2LULul−1 RNase inhibitor (Thermo Fisher, cat. no. AM2694) in 1X PBS ...
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bioRxiv - Neuroscience 2023Quote: ... p21) and pAAV target plasmid in a 4:1:1:2 molar ratio by use of Lipofectamine 2000 (ThermoFisher) according to company protocol ...
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bioRxiv - Neuroscience 2024Quote: ... The passage was performed twice per week (1:2 or 1:3) using Accutase (Cat #A1110501, Thermo Fisher Scientific) by vigorously breaking pellets to remove neuronal cells ...
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bioRxiv - Biochemistry 2024Quote: ... and p976 were packaged in HEK-293T cells at a ratio of 2:1:1 by Lipofectamine 2000 (Invitrogen). The ratio of liposome to DNA was 2:1 ...
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bioRxiv - Neuroscience 2024Quote: ... pCMVR8.74 and pLVX-EF1α-mitoSRAI-IRES-Puro at a 1:1:2 molar mass ratio using Lipofectamine 3000 (Invitrogen). The next day ...
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bioRxiv - Molecular Biology 2023Quote: ... Inoculated cells were incubated for 1 h at 37°C and then overlaid with a 1:1 mixture of 1.2% Oxoid agar and 2× DMEM (Gibco) with 10% (vol/vol ...
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bioRxiv - Developmental Biology 2023Quote: ... washed 1 x 30 min at room temperature in PBSTE-1% BSA supplemented with 2% phalloidin-AF488 (Invitrogen A12379), washed 4 x 30 min at room temperature in PBSTE-1% BSA and washed 2×30’ at room temperature in PBSTE ...
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bioRxiv - Immunology 2022Quote: ... OT-I T-cells (∼400,000 in 1 mL growth media) were loaded with 1 μM Fura-2 AM (F1221, ThermoFisher) for 40 min RT ...
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bioRxiv - Molecular Biology 2023Quote: ... for 1-2 h at room temperature (1:1000) for viral titration or an anti-GFP antibody (A11122, Invitrogen) for measurement of oxGFP-positive cells ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged with 80-90% confluence at a split ratio of 1:2-1:4 using TrypLE (Gibco) for 8 min at 37°C ...
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bioRxiv - Physiology 2023Quote: ... myotubes were loaded with Fura-2 QBT by incubation with 1:1 Gibco Opti-MEM (Life Technologies; Carlsbad, CA) and Fura-2 QBT dissolved in modified Krebs-Ringer Buffer with HEPES (mKRBH ...
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bioRxiv - Cancer Biology 2019Quote: ... All TaqMan probes were 5′-6-carboxyfluorescein (FAM) and 3′-6-carboxy-N,N,N′,N′-tetramethylrhodamine (TAMRA) labeled (Applied Biosystems, US) except TATA-binding protein (TBP ...
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bioRxiv - Molecular Biology 2020Quote: ... (G4S)6 Linker-VH/CH1 and (G4S)6 Linker-VH/CH1-(G4S)6 Linker-VH/CH1 sequences were synthesized using GeneArt® gene synthesis service (ThermoFisher Scientific).
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bioRxiv - Genomics 2021Quote: ... cells were seeded in 6-well plates or 6 cm dishes and reverse transfected with 5 nM Silencer Select siRNAs (Thermo Fisher Scientific) using RNAiMAX (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2019Quote: ... iPSC cultures at ~90% confluence in 6-well-plates were treated with 6 μM CHIR-99021 (SelleckChem) in RPMI 1640 medium supplemented with B27 supplements (Thermo Fisher Scientific) for 2 days to induce mesoderm specification ...
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Immunoresolvents Support Skeletal Myofiber Regeneration via Actions on Myeloid and Muscle Stem CellsbioRxiv - Immunology 2020Quote: Bone marrow was collected from tibias and femurs of 4-6 mo female C57BL/6 mice and cultured for 7 days at 37°C and 5% CO2 in DMEM (Gibco,11995-073) supplemented with 10% FBS ...