Labshake search
Citations for Thermo Fisher :
2151 - 2200 of 10000+ citations for 6 Benzothiazolamine 2 1 methylpropyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2024Quote: ... plasmid DNA into 1 × 106 HEK293 cells plated per well of a 6-well plate using Lipofectamine 2000 transfection reagent (Invitrogen). Each well was transfected with 2 µg of total plasmid DNA (50 ng Cg4-puro ...
-
bioRxiv - Immunology 2023Quote: ... One day prior to the co-culture 70- and 20-4_1BBζ cells were defrosted and cultured in a density of 1 × 10^6 cells/mL in RPMI containing1X GlutaMAX (Gibco), 1X P/S and 10% FCS supplemented with 10 ng/mL IL7 and IL15 overnight ...
-
bioRxiv - Molecular Biology 2023Quote: ... Samples that were subjected to RNase A digestion to detect nuclease-resistant disomes were incubated for 1 h at 25 °C with 15 μl of RNase A (1:1,000 dilution) then treated with 6 μl of SUPERase-In RNase Inhibitor (Thermo Fisher). Sucrose gradients of 10-50% were prepared using a Gradient Master 108 (Biocomp ...
-
bioRxiv - Microbiology 2023Quote: ... around 3 to 5 μg total RNA from samples were loaded onto a 6% formaldehyde-containing 1% agarose gel in the fume hood with RNA Millenium Marker (Ambion) as RNA ladder ...
-
bioRxiv - Molecular Biology 2023Quote: ... HEK293T cells were seeded in 6-well plates and transfected with a total of 1 µg of plasmid DNA per well using Lipofectamine 2000 (Invitrogen) as transfecting reagent ...
-
bioRxiv - Plant Biology 2023Quote: ... for 40 cycles (15 seconds at 95°C, 1 minute at 60°C) in a QuantStudio 6 Flex instrument (ThermoFisher). Percentage of input was calculated as 100* e(CtIN – log2(DF ...
-
bioRxiv - Cell Biology 2023Quote: 6-well plates were coated with a solid base of 1 mL of agar (0.8% in complete DMEM) (Thermo Fisher). On top of this solid base ...
-
bioRxiv - Neuroscience 2024Quote: ... in 6-well tissue culture plates containing 1 ml prewarmed culture medium (42% MEM, 25% Basal Medium Eagle; Thermo Scientific), 25% Normal Horse Serum ...
-
bioRxiv - Microbiology 2021Quote: ... Vero E6 cells were inoculated with 10-fold dilutions of SARS-CoV-2 for 1 h and overlaid with 0.8% (w/v) Avicel medium (1 x MEM Temin’s modification (Gibco), 0.8% (w/v ...
-
bioRxiv - Cancer Biology 2020Quote: ... The HDFC was dissolved with a 1:1 mixture of 2 mg/ml collagenase type2 and trypsin (Gibco), and cell numbers were quantified using hemocytometry ...
-
bioRxiv - Microbiology 2019Quote: ... Inoculum was removed and 2 mL of overlay media (MEM containing 1% methylcellulose, 10% FBS, 1% GlutaMAX (Gibco), 1% HEPES ...
-
bioRxiv - Neuroscience 2022Quote: ... and then switched to neural induction medium (Advanced DMEM/F12 (1:1) supplemented with 2 mM GlutamaxTM (Gibco), 2% B27 without vitamin A (Life technologies) ...
-
bioRxiv - Molecular Biology 2020Quote: ... were similarly cultured but in DMEM:Ham’s F12 (1:1) medium with 2 mM glutamine and 10 % fetal-calf serum (Invitrogen). FugeneHD (Promega ...
-
bioRxiv - Molecular Biology 2021Quote: ... Thawed CM aliquots were mixed 1:1 with basal media (BM) consisting of 2% B-27 (Gibco 12587001), 10 mM Nicotinamide (Sigma-Aldrich N0636) ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were fixed for 1 minute in 1 ml PBS 2% formaldehyde (Thermo-Fisher Scientific, Cat. No. 28906) before being fixed for a further 14 minutes in fresh PBS 2% formaldehyde ...
-
bioRxiv - Neuroscience 2024Quote: ... Wisteria floribunda lectin (1:1000, B-1355-2, Vector) was visualized using Streptavidin 568 (1:500, S11226, Invitrogen).
-
bioRxiv - Immunology 2024Quote: ... mixing with 2 million CD45.1+ T cells at 1:1 ratio and labeling with CellTrace Violet dye (Invitrogen), into Rag1−/− mice ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were resuspended at 2 x 106 cells/ml in assay medium containing DMEM/F12 (1:1, Gibco) supplemented with 5% horse serum (Invitrogen) ...
-
bioRxiv - Developmental Biology 2023Quote: ... and plated 1×106 and 2×106 cells on Geltrex (Gibco, 1:100 DMEM/F12, Thermo Fisher Scientific)- coated 60 mm and 10 cm plates respectively ...
-
bioRxiv - Neuroscience 2024Quote: ... Alexa Fluor (AF) 647-conjugated streptavidin (1:300 diluted from 2 mg ml−1 stock solution, Invitrogen S32357) was added ...
-
bioRxiv - Microbiology 2022Quote: ... purified CD4+ T cells were maintained for two days in complete RPMI 1640 medium containing recombinant interleukin-2 (IL-2) (300 IU/ml) and phytohemagglutinin (1 μg/ml, Thermo Fisher Scientific), followed by 3 days in the presence of IL-2 alone ...
-
bioRxiv - Microbiology 2021Quote: ... cells were fixed with 4% PFA for 30 minutes and stained for intracellular ENV with 1A1D-2 (1/500) monoclonal antibody and anti-mouse 647-conjugated antibody (2 μg/ml, Invitrogen Cat# A21235).
-
bioRxiv - Molecular Biology 2019Quote: Digested peptides were dissolved in 40 μL of 0.1% formic acid containing 2% (v/v) acetonitrile and 2 μL were injected into an Easy-nLC 1000 system (Thermo Fisher Scientific). Peptides were separated on an Acclaim PepMap™ RSLC column (15 cm × 50 μm inner diameter ...
-
bioRxiv - Immunology 2020Quote: ... for 2 days, then passaged 1:2 and cultured overnight in R10 medium (1X Roswell Park Memorial Institute (RPMI, (Life Technologies SAS), 10% fetal calf serum ...
-
bioRxiv - Immunology 2020Quote: ... 25mM HEPES (2(−4-(2-hydroxyethyl)-1-piperanzinyl) ethansulfonacid) and supplemented with 10% (v/v) fetal bovine serum (FBS; Gibco, ThermoFisher Scientific), 10.000 U/mL (v/v ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... 5 mM DTT, 50 mM HEPES, 3 mM MgCl2, 2 mM PMSF, 1 Pierce™ protease inhibitor mini tablet/2 L; Thermo Scientific). The lysate was sonicated then incubated with 500 units/1 L culture Benzonase Nuclease (Sigma-Aldrich ...
-
bioRxiv - Biophysics 2021Quote: ... and HA-mUNC79 plasmids or their various mutants (in a modified BacMam expression vector 27) at a ratio of 2:1:1:1 using Lipofectamine 3000 (Thermo Fisher Scientific) and incubated for 24 hours before recording ...
-
bioRxiv - Immunology 2019Quote: ... in a 1:1 beat to T-cell ratio and cultured in 2 ml (24WP) or 1 ml (48WP) RPMI 1640 (Thermo Fisher, 21875) supplemented with 10% Fetal Bovine Serum ...
-
bioRxiv - Cell Biology 2022Quote: HUVECs expressing PIK3CA p.H1047R or PIK3CA wt were transfected with a Silencer Select siRNA targeting to TGFA (ID: s14053) or negative control siRNAs (#1 and #2 mixed in ratio 1:1; all siRNA oligonucleotides from Thermo Fisher Scientific) as previously described (58) ...
-
bioRxiv - Physiology 2023Quote: ... Cells were grown on DMEM/Hams F12 with 2% fetal bovine serum and standard cell culture hormones (Dexamethasone 1 1 mM; mM, T3 1 μM, ITS 100X Gibco cat # 41400045) on 6-well ...
-
bioRxiv - Immunology 2022Quote: ... 0.5% 2-mercaptoethanol (2-ME) (GIBCO) and 1% HEPES (GIBCO) ...
-
bioRxiv - Developmental Biology 2021Quote: ... with 50-100 nl of 1 mM 5-ethynyl-2’-deoxyuridine (EdU, Invitrogen) at stage 41 ...
-
bioRxiv - Cell Biology 2020Quote: ... F(ab’)2 goat anti-mouse Alexa Fluor 350 (Molecular Probes 1:1000) and F(ab’)2 goat anti-mouse Alexa Fluor 633 (Molecular Probes ...
-
bioRxiv - Neuroscience 2021Quote: ... The 2° antibody used was anti-chicken Alexa 488 (1:500, Life Technologies). Confocal image stacks were acquired on a Leica SP8 ...
-
bioRxiv - Neuroscience 2021Quote: ... We used a MAP-2 polyclonal antibody (PA5-17646 Invitrogen, 1:1000 dilution) to stain for dendrites ...
-
bioRxiv - Biophysics 2022Quote: ... NPE-caged ATP (adenosine 5’-triphosphate, P3- (1-(2-nitrophenyl ethyl) ester) (Invitrogen) dissolved in attachment buffer was photolyzed in the AFM chamber using an UV light source at 340 nm ...
-
bioRxiv - Cell Biology 2022Quote: ... (B-5-1-2 coupled to Alexa-488, Thermo Fisher or DM1A, Sigma), rat anti-tyrosinated tubulin (YL1/2 ...
-
bioRxiv - Immunology 2022Quote: ... 1-2 million PBMCs were plated into 12 well plate (Nunc, Thermo Fisher) at 37°C and 5% CO2 and allowed to adhere for two hours in 1 mL of RPMI 1640 (Hyclone ...
-
bioRxiv - Immunology 2022Quote: ... 1-2 million PBMCs were plated into 12 well plate (Nunc, Thermo Fisher) at 37°C and 5% CO2 and allowed to adhere for two hours in 1 mL of RPMI 1640 (Hyclone ...
-
Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: ... gene using RNase P probe purchased from Applied Biosystems and RNase P primer 1 and RNase P primer 2 purchased from Invitrogen. The final concentration of primers was 500 nM each and probe was 250 nM ...
-
bioRxiv - Immunology 2020Quote: ... mouse CD68 (Thermo Fisher Scientific, USA, 1:100, 2 h at 37°C) for macrophages and pDC’s were identified by co-staining of PE conjugated mouse anti-human CD123 (BD Biosciences ...
-
bioRxiv - Bioengineering 2021Quote: ... were maintained in a 2:1 mix of FreeStyle293:Expi293 Expression medium (ThermoFisher) and grown at 37 °C and 8% CO2 while shaking at 120 rpm ...
-
bioRxiv - Bioengineering 2021Quote: DNA sizes were first confirmed by running 1-2% Agarose E-Gel (Invitrogen). Then ...
-
bioRxiv - Microbiology 2021Quote: ... containing 100µl Schneider’s media (Experimental Replicates 1 and 2) or RPMI (ThermoFisher Scientific) (Experimental Replicates 3 and 4 ...
-
bioRxiv - Neuroscience 2020Quote: ... 2 mM L-glutamine and 1% MEM non-essential amino acids (ThermoFisher Scientific). Cells were collected 72 h after transfection with 125 pmol Stealth siRNA using Lipofectamine® 2000 (Invitrogen ...
-
bioRxiv - Cancer Biology 2021Quote: Recombinant aFGF/FGF-1 and bFGF/FGF-2 were purchased from Thermo Fisher Scientific (13241-103 and PHG0024) ...
-
bioRxiv - Cancer Biology 2019Quote: ... or mouse monoclonal antibody against HK-2 (1:100; Thermo Fisher, MA5-15679). After repeated washes ...
-
Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... 2 mM GlutaMAX and 1 % antibiotic-antimycotic (all Thermo Fisher Scientific, Epsom, UK). SH-SY5Y cells stably expressing YFP-alpha-synuclein were obtained by lentiviral transfection using 3rd generation lentiviruses (Addgene constructs ...
-
bioRxiv - Molecular Biology 2021Quote: ... 10 mmol/l 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Gibco, Germany #15630056), 2.5 ng/ml human fibroblast growth factor-basic (hFGF2 ...
-
bioRxiv - Microbiology 2020Quote: ... 1 mL of culture was mixed with 2 volumes of RNAlater (Invitrogen, USA) for five minutes at room temperature ...