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Citations for Thermo Fisher :
2251 - 2300 of 10000+ citations for Recombinant Human High Mobility Group Box 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 3000 Plp1+ cells from each group (control, D1, D3, D7) were collected into 96-well plates (Life Technologies) coated with BSA (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... All parasite cultures were maintained in blood group O RBCs at 4% hematocrit in RPMI-1640 medium (Gibco) containing 27 nM NaHCO3 (Sigma) ...
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bioRxiv - Cancer Biology 2024Quote: ... Epifluorescent images were captured using an EVOS FL inverted microscope by American Microscope Group (AMG) (Thermo Fisher Scientific) with a 40X oil objective and GFP ...
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bioRxiv - Molecular Biology 2024Quote: ... A 100% lysis rate was set using the A541nm from the group of 0.6% RIPA (Thermo Fisher Scientific) lysis buffer.
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bioRxiv - Molecular Biology 2024Quote: ... in HepG2 medium (Advanced MEM minus L-Glutamine [Gibco] + 10% [v/v] HI-FBS [Gibco] + 1% [v/v] penicillin-streptomycin + 2 mM Glutamax [Gibco] and 0.1% [v/v] amphotericin B). Once the HepG2 or HC-04 cells were grown to the desired concentration ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μg of total RNA was reverse transcribed using a High-Capacity Reverse Transcription Kit (Applied Biosystems) and ultimately re-suspended in 200 µl of water ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μg of RNA was then reverse transcribed using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Quantitative PCR was performed with 10ng of cDNA ...
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bioRxiv - Cancer Biology 2019Quote: ... and 1 μg was reverse transcribed into cDNA using High Capacity cDNA Reverse Transcription Kit (Thermo Fisher). Each sample was amplified in triplicate using custom-designed primers (Sigma ...
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bioRxiv - Systems Biology 2020Quote: ... 0.6-1 μg of total RNA is reverse transcribed using High Capacity cDNA Kit (Applied Biosystems inc.). qRT-PCR was performed using SYBR Green PCR Master Mix (Applied Biosystems inc. ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA was synthesized from 1 μg total RNA using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) using Oligo(dT)15 ...
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bioRxiv - Neuroscience 2020Quote: ... Full-length age-1 cDNA was PCR-amplified using Phusion High-Fidelity DNA Polymerase+GC buffer (Thermofisher) using total cDNA prepared from N2 mRNA (Ampliscribe) ...
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bioRxiv - Microbiology 2021Quote: ... 1 µg of RNA was reverse transcribed using the High-Capacity cDNA Reverse Transcription kit (Applied Biosystems) following the manufacturer’s instructions and gene specific primers against the T3DCD S1 gene segment ([5’-TGGCGAGATTATTCCCTGAC-3’] ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μg of RNA was reverse transcribed using high-capacity cDNA Reverse transcription kit (Applied Biosystems™). Quantitative PCR was performed on 15 ng cDNA using PowerUp SYBR Green Master Mix (Applied Biosystems™ ...
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bioRxiv - Neuroscience 2020Quote: ... 1 μg of RNA was reversed transcribed using the High Capacity RNA-to-cDNA kit (ThermoFisher, #4387406). The All-in-One qPCR Mix (GeneCopoeia ...
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bioRxiv - Bioengineering 2021Quote: ... cDNA was prepared using 1 μg RNA with the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). SYBR green based qPCR was performed in triplicate using human primers to Aggrecan (ACAN) ...
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bioRxiv - Genomics 2022Quote: ... and 1 μg was used for cDNA synthesis using the High-Capacity cDNA Reverse Transcription Kit (ThermoFisher). RT-PCR was done using IQ SYBR green (Biorad ...
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bioRxiv - Synthetic Biology 2019Quote: Luc14 and Gal4-EED/luc HEK293 cells were grown in Gibco DMEM high glucose 1× (Life Technologies) with 10% Tet-free Fetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2019Quote: ... cDNA was synthesised from 1 μg of RNA using High-capacity cDNA Reverse Transcription kit (Applied Biosystems) and gene expression was determined by SYBR-green RT-PCR on the Lightcycler 480 (Roche) ...
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bioRxiv - Neuroscience 2020Quote: ... Up to 1 μg of RNA was converted to cDNA (Applied Biosystems High Capacity cDNA Conversion Kit) and was diluted to 5 ng/μl assuming 100% conversion efficiency.
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bioRxiv - Genetics 2020Quote: ... 1 µg of RNA was reverse transcribed using a High Capacity RNA-to-cDNA Kit (Applied Biosystems) following manufacturer’s protocols ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was prepared from 1 g RNA using a high-capacity RNA-to-cDNA kit (Applied Biosystems), according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... cDNA was synthesized from 1 µg of RNA using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) with random primers.
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bioRxiv - Bioengineering 2022Quote: ... China) were cultured in a high-glucose (4.5g·L-1) Dulbecco’s Modified Eagles’ Medium (DMEM, Gibco, Carlsbad, CA) with a supplementary of 10% FBS and 1% P/S ...
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bioRxiv - Neuroscience 2022Quote: ... Undiluted total RNA (1 ug) was reverse transcribed with the High-Capacity cDNA Reverse Transcription Kit (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were cultured at high density and split every two days using 1-2 min TrypLE (Gibco) incubation ...
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bioRxiv - Neuroscience 2024Quote: ... 1-2µg of RNA was used for cDNA synthesis using High-capacity reverse transcriptase kit (ThermoFisher Scientific). Real-time qRT-PCR was performed on the QuantStudio 6 Flex thermocycler using PowerUp SYBR green master mix (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was generated from 1 µg RNA using the High-Capacity RNA-to-cDNA kit (Applied Biosystems) as per manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... Passage 1 of viruses was used to express the proteins in High Five™ Cells (Thermo Fisher). The cells harvested 48 hours post inoculation were lysed in 50 mM Tris.HCl pH 8 ...
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bioRxiv - Cell Biology 2024Quote: ... 1 μg of total RNA was reverse transcribed with High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Real-time quantitative PCR was performed to analyze relative gene expression levels using SYBR Green Master mix (Applied Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... Hepa 1-6 and HuH-7 were grown in Dulbecco’s modified Eagle’s medium–high glucose (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Neuroscience 2023Quote: ... 1 µg of RNA was reverse transcribed to cDNA using High-capacity reverse transcription kit (Applied Biosystems). RT-PCR was carried out using the following primers ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 μg of total RNA was reverse transcribed using a High-Capacity Reverse Transcription Kit (Applied Biosystems). RT-PCR was performed in triplicate using SYBR Green PCR Master Mix (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... cDNA was generated from 1 μg of RNA with High-capacity cDNA Reverse Transfection kit (Applied Biosystems). Real-time qPCR was performed with Syber Green I Master kit (Roche ...
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bioRxiv - Microbiology 2024Quote: ... an additional step was included using Pierce high-capacity endotoxin removal spin 1 ml column (Thermo Scientific) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... cDNA was prepared from 1 µg RNA using a high-capacity RNA-to-cDNA kit (Applied Biosystems), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... HeLa cells were grown in DMEM high glucose with GlutaMax and 1 mM pyruvate (ThermoFisher Scientific, 31966021), supplemented with penicillin-streptomycin (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Cell pellets were incubated with a 1:200 dilution of human cTnT primary mouse antibody (ThermoFisher) overnight at 4°C ...
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bioRxiv - Immunology 2019Quote: ... cells were stained with a PE-conjugated anti-human IgG1(Fc) secondary antibody (1:50) (ThermoFisher) for additional 15min ...
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bioRxiv - Neuroscience 2019Quote: ... or 10 μM of freshly prepared human synthetic amyloid-β peptide 1-42 (Aβ42, Life technologies) for 24 h as described (Kiyota et al. ...
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bioRxiv - Microbiology 2020Quote: The human acute leukemia monocyte cell line (THP-1) was cultivated in RPMI 1640 medium (Invitrogen) supplemented with 10% heat-inactivated FBS (Thermo Scientific Hyclone ...
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bioRxiv - Biochemistry 2022Quote: ... The ORF of human MYBL2/B-Myb isoform 1 (NM_002466.4) was cloned into pcDNA3.1+ (ThermoFisher Scientific) and fused with an N-terminal Flag tag ...
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bioRxiv - Molecular Biology 2022Quote: ... Mouse-anti-human H3K9/14/18/23/27ac (Thermo Fisher Scientific, Carlsbad, CA, USA; 1:500) was used to detect this histone modification in HPdLF and goat-anti-Mouse-Cy5 (Jackson ImmunoResearch ...
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bioRxiv - Microbiology 2021Quote: ... Alexa488- or Rhodamine-conjugated goat anti-human secondary antibody was added (1/500 dilution) (Thermo Fisher), and incubated for 2~3 hr at room temperature in the dark ...
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bioRxiv - Molecular Biology 2021Quote: ... Immortalized human fibroblasts were cultured in DMEM (sigma) supplemented with 1% of penicillin and streptomycin (Gibco) and 10% fetal bovine serum (Corning ...
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bioRxiv - Immunology 2020Quote: ... plates were incubated with goat polyclonal anti-human IgG HRP-conjugate (1/1000; Thermo Fisher Scientific) for 1 hour at RT ...
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bioRxiv - Immunology 2021Quote: ... plates were incubated with a goat anti-human IgG HRP-conjugate (1/1000; Thermo Fisher Scientific). For IgG subclasses and IgM detection ...
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bioRxiv - Immunology 2021Quote: Human THP-1 cells (ATCC® TIB-202™) were cultured in RPMI media (22400089, ThermoFisher) supplemented with 1% (v/v ...
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bioRxiv - Immunology 2021Quote: ... diluted 1:3000 in blocking buffer or HRP-labelled goat anti-human IgM (Thermo Fisher Scientific) diluted 1:4000 at 37°C for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... and 1:5,000 diluted goat anti-human-IgG Fc horseradish peroxidase (HRP) conjugated secondary antibodies (Invitrogen) were added ...
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bioRxiv - Developmental Biology 2022Quote: ... CD31-APC with human specificity for HUVEC experiments (1:500, Thermo Fisher Scientific, 17-0319-42) together with its IgG1 kappa APC-Isotype control (1:500 ...