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Citations for Thermo Fisher :
2051 - 2100 of 10000+ citations for Recombinant Human High Mobility Group Box 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... The resultant FLAG-tagged STAT3 constructs were transfected into 293T using lipofectamine 3000 (Thermo Fisher Scientific). 48 hours after transfection cells were lysed (50mM Tris ...
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bioRxiv - Genetics 2019Quote: ... Proteins tagged with the V5 epitope (Hxk2, Reg1) were detected with the Anti-V5 probe (Invitrogen) diluted 1:1,000 ...
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bioRxiv - Plant Biology 2022Quote: ... GST and GST-tagged SAMS were incubated with 40 μl GST magnetic beads (Thermo Fisher Scientific) for 1 h at 4°C and then these beads were washed three times with lysis buffer ...
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bioRxiv - Microbiology 2023Quote: Mono-Fc tagged PvDBP-RII used for SAXS experiments was expressed in Expi293 cells (Thermo Scientific) as described above and purified using the CaptureSelect C-tagXL Affinity Matrix (Thermo Scientific) ...
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bioRxiv - Genetics 2023Quote: ... Flag-tagged proteins were immunoprecipitated with anti-Flag magnetic agarose (Pierce Anti-DYKDDDDK Magnetic Agarose, ThermoFisher), according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... Hexa-histidine tagged mTHSD7A fragments were purified under native conditions using Ni-NTA resin (Thermo Scientific) applying the batch method according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Rab7a and LAMP1 wt and mutant molecules tagged with EGFP using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... WRN Halo-tagged HCT-116 cells were seeded in FluoroBrite DMEM (Thermo Fisher, cat. no. 1896701) supplemented with 10% FBS (Corning) ...
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bioRxiv - Immunology 2024Quote: ... the cells were probed with donkey anti-mouse secondary antibody tagged with Alexa Fluor 568 (Invitrogen), for 1 h in dark ...
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bioRxiv - Molecular Biology 2024Quote: ... Tagged gDNA was enriched using Dynabeads™ MyOne™ Streptavidin C1 (Thermo Fisher Scientific, Cat. 65001). The beads were allowed to room temperature while being resuspended on a HulaMixer™ Sample Mixer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: Samples were analyzed by a high-resolution/high-accuracy LCMS/MS (Lumos Fusion, Thermo Fisher). Peptides were desalted using ZipTips (Sigma Millipore ...
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bioRxiv - Genomics 2023Quote: ... were quantified using Qubit dsDNA High Sensitivity and RNA High Sensitivity Assay kits (ThermoFisher Scientific) and stored in aliquots at -20°C.
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bioRxiv - Immunology 2023Quote: Samples were analyzed by high resolution/high accuracy LC-MS/MS (Lumos Fusion, Thermo Fisher). Peptides were separated using direct loading onto a packed-in-emitter C18 column (75um ID/12cm ...
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bioRxiv - Neuroscience 2021Quote: ... were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with high glucose (1:1 v/v, Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10 % fetal bovine serum ...
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bioRxiv - Physiology 2023Quote: ... cells were resuspended in plating medium (DMEM high glucose #1-26P02-K, M199 EBS #1-21F01-I; both from BioConcept, 10% horse serum (#16050130; Thermo Fisher Scientific), 5% fetal calf serum (#10270-106 ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mL min-1) and identity of peptides confirmed by high-resolution mass (Q Exactive Orbitrap LC-MS/MS (Thermo Fisher Scientific).
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bioRxiv - Genomics 2023Quote: ... coated dishes in N2 medium (high-glucose DMEM, 1% N-2, 2% B-27 and 1% penicillin/streptomycin, from Thermo Fisher Scientific) at a density of 2×105 cells/cm2 ...
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bioRxiv - Microbiology 2020Quote: ... we used human monoclonal antibodies produced recombinantly in human Expi293F cells (Life Technologies) as described before (Fang et al ...
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bioRxiv - Cell Biology 2021Quote: ... anti-human IgG-Alexa647 and anti-human IgG-Alexa568 were obtained from Invitrogen, anti-acetylated tubulin (T7451 ...
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bioRxiv - Cell Biology 2020Quote: ... and human GAPDH and human KIF18A Taqman probes and primers (Thermo Fisher Scientific) were used for reverse transcription and qRT-PCR ...
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bioRxiv - Neuroscience 2020Quote: ... and subjected to human total tau ELISA (human tau: # KHB0042, Thermo Fisher Scientific) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... slides were washed thrice for 5 min in PBS before incubation with Alexa 594 goat-anti rabbit secondary antibody at 1:1,000 and Alexa Fluor 488 goat anti-human IgG(H+L) at 1:500 (both Invitrogen; in PBS) for 2 h at RT ...
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bioRxiv - Cell Biology 2021Quote: ... EpiLCs were induced by plating 1.5 x 106 ESCs on a 10 cm dish coated with human plasma fibronectin (16.7 mg/ml) in the 1:1 mix of DMEM/F12 (Gibco, 11302-33) and Neurobasal medium containing N2 supplements ...
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bioRxiv - Evolutionary Biology 2020Quote: ... a formulation optimal for human fibroblasts [(Dickson et al., 2000): 1:1 (vol/vol) medium M199 and medium M106 (ThermoFisher Scientific) + 15% BCS + 10 ng/ml EGF + 0.4 ug/ml hydrocortisone + 1% Penicillin-Streptomycin] ...
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bioRxiv - Microbiology 2023Quote: ... and was then incubated (1:1000 dilution) for 1 hour with a mouse anti-human E-Cad ectodomain antibody (HECD-131700, 1:1000 dilution; Life Technologies). After two washes ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies used in this study were all anti-human: STING (1:1000, CST, #13647 or 1:500, Thermo Fisher, MA526030), UBIQUITIN (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 h at room temperature and then exposed to antibodies specific for human PD-1 (ThermoFisher Scientific; 14-9969-82), Iba1 (Wako Chemicals ...
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bioRxiv - Bioengineering 2023Quote: ... primary T cells were activated and stimulated with a 1:1 ratio of anti-human CD3/CD28 magnetic Dynabeads (Thermo Fisher) to cells in X-vivo 15 medium supplemented with 5% human AB serum (Heat inactivated) ...
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bioRxiv - Biophysics 2024Quote: ... primary stained with goat anti-Syndecan-1 antibody and mouse anti-human choriogonadotropin (β-hCG) antibody (#14-6508-82, 1:500 dilution in the buffer solution, ThermoFisher), and secondary stained as previously described ...
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bioRxiv - Microbiology 2022Quote: ... Samples were washed in blocking buffer thrice and stained with 1:1000 dilution of Alpaca anti-Rabbit IgG Nano (VHH) Recombinant Secondary Antibody conjugated with Alexa Fluor™ 647 (Invitrogen SA5-10327), Goat anti-Mouse IgG conjugated with Alexa Fluor™ Plus 488 (5 μg/mL final concentration ...
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bioRxiv - Cancer Biology 2022Quote: One million of BMDCs cultures in the absence or presence of 10 ng/ml of recombinant MDK (indicate provider and origin) were incubated with 1 μg/ml of Ovalbumin-AF594 conjugate (Thermo Fisher Scientific; O34781) for 30 min at 37°C ...
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bioRxiv - Microbiology 2023Quote: ADCP was measured using a flow cytometry-based phagocytosis assay.98 Biotinylated recombinant HIV-1 gp120 from strain CN54 (Acro Biosystems; catalog #GP0-V182E6) was combined with fluorescent NeutrAvidin beads (Life Technologies; catalog #F8776) at a concentration of 1 µg protein per µl bead and incubated overnight at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... the head group-labeled fluorescent dye Texas Red 1,2-dihexadecanoyl 3-phosphoethanolamine (TR-DHPE) (Molecular Probes, Eugene, OR) was first dissolved in chloroform and subsequently added to lipid spreading solutions at 0.5 mol% ...
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bioRxiv - Immunology 2021Quote: Groups of 10 embryos were lysed and homogenised using a 27-gauge needle in 500 μl Trizol (Invitrogen) for RNA extraction ...
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bioRxiv - Cell Biology 2021Quote: ... the viral supernatants from the test groups were added into the deep well plate (KingFisher™Thermo Scientific) along with a lysis buffer containing the following components - 260 μL ...
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Therapeutic base and prime editing of COL7A1 mutations in recessive dystrophic epidermolysis bullosabioRxiv - Bioengineering 2021Quote: ... equal amounts of proteins from each group were loaded onto Nupage Novex Bis-Tris Gels (Thermo Fisher Scientific), and electrophoresis was performed using an X-cell SureLock Mini-Cell (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2021Quote: ... Endotoxin contamination was assessed for all NP groups using Pierce™ LAL Chromogenic Endotoxin Quantitation Kit (ThermoFisher Scientific) according to manufacturer’s guidelines.
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bioRxiv - Bioengineering 2020Quote: ... PBMCs were seeded on top of cell-free GelMA discs (n = 5 per group) with a density of 2.5 ⨯ 105/ml and cultured in 0.5 ml RPMI-1640 + GlutaMAX (Gibco) supplemented with 10% FBS and 1% P/S for 24 hours in 48-wells plates ...
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bioRxiv - Neuroscience 2021Quote: The HEK293T cells were divided into four groups and transfected with plasmids by Lipofectamine 2000 (11668-027, Invitrogen). The four groups (tTAN ...
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bioRxiv - Genomics 2021Quote: ... 2 × 105 cells from each group were maintained in erythroid differentiation media (IMDM media (Fisher Scientific, Hampton, NH) containing 1 ng/ml hIL-3 ...
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bioRxiv - Immunology 2022Quote: Two groups of PBMCs (5×106 cells/ml) were stimulated with 1μg/ml anti-CD3/CD28 antibodies (Invitrogen) at 37°C under 5% CO2 for 18 hours in RPMI-1640 medium ...
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bioRxiv - Genetics 2020Quote: mRNA was collected from groups of 10 whole larvae (n=3–4 replicates per line) using Trizol (ThermoFisher) and reverse transcribed to cDNA using SuperScript III Reverse Transcriptase (ThermoFisher) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Samples of the visceral yolk sac (n=6/group) were immersed in RNA Later solution (Invitrogen, MA, USA) and frozen at −20°C until further processing ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was extracted from larvae (groups of 10) using TRIzol according to manufacturer’s instructions (Invitrogen; 15596-018). RNA samples were then subjected to DNase treatment according to manufacturer’s instructions (Ambion ...
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bioRxiv - Microbiology 2021Quote: ... The viral supernatants from the test groups were added into the deep well plate (KingFisher™ Thermo Scientific) along with a lysis buffer containing the following components - MagMAX™ Viral/Pathogen Binding Solution ...
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bioRxiv - Immunology 2020Quote: Groups of 10 embryos were lysed and homogenised using a 27-gauge needle in 500 μl Trizol (Invitrogen) and RNA extracted as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Isolated cells pooled from multiple mice per group were processed using an Invitrogen mirVana miRNA Isolation kit (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 484 bp for group 2) was excised using a 2% E-Gel SizeSelect Agarose Gels (Thermo Fisher Scientific). The sequence library was adjusted to 10 pM (assuming 1 bp DNA has a molecular weight of 660 g/mol ...
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bioRxiv - Neuroscience 2023Quote: ... 3000 Plp1+ cells from each group (control, D1, D3, D7) were collected into 96-well plates (Life Technologies) coated with BSA (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... All parasite cultures were maintained in blood group O RBCs at 4% hematocrit in RPMI-1640 medium (Gibco) containing 27 nM NaHCO3 (Sigma) ...