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Citations for Thermo Fisher :
2201 - 2250 of 10000+ citations for Recombinant Human High Mobility Group Box 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... GST and GST-tagged SAMS were incubated with 40 μl GST magnetic beads (Thermo Fisher Scientific) for 1 h at 4°C and then these beads were washed three times with lysis buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... Tagged gDNA was enriched using Dynabeads™ MyOne™ Streptavidin C1 (Thermo Fisher Scientific, Cat. 65001). The beads were allowed to room temperature while being resuspended on a HulaMixer™ Sample Mixer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... the cells were probed with donkey anti-mouse secondary antibody tagged with Alexa Fluor 568 (Invitrogen), for 1 h in dark ...
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bioRxiv - Microbiology 2023Quote: Mono-Fc tagged PvDBP-RII used for SAXS experiments was expressed in Expi293 cells (Thermo Scientific) as described above and purified using the CaptureSelect C-tagXL Affinity Matrix (Thermo Scientific) ...
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bioRxiv - Genetics 2023Quote: ... Flag-tagged proteins were immunoprecipitated with anti-Flag magnetic agarose (Pierce Anti-DYKDDDDK Magnetic Agarose, ThermoFisher), according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... Hexa-histidine tagged mTHSD7A fragments were purified under native conditions using Ni-NTA resin (Thermo Scientific) applying the batch method according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Rab7a and LAMP1 wt and mutant molecules tagged with EGFP using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... WRN Halo-tagged HCT-116 cells were seeded in FluoroBrite DMEM (Thermo Fisher, cat. no. 1896701) supplemented with 10% FBS (Corning) ...
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bioRxiv - Molecular Biology 2024Quote: ... Tissues from Knock-in LAP-tagged PAF1C flies were detected with anti-V5 tag (Invitrogen, R96025) 1:500 in BBX incubated overnight at 4°C with nutation ...
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bioRxiv - Microbiology 2024Quote: ... ∼300RU of each Fc-tagged KIR2DL1 and KIR2DS1 were captured via Protein A/G (ThermoFisher Scientific) pre-immobilized on a CM5 sensor (∼1500 RU on each flow cell) ...
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bioRxiv - Cancer Biology 2020Quote: Samples were analyzed by a high-resolution/high-accuracy LCMS/MS (Lumos Fusion, Thermo Fisher). Peptides were desalted using ZipTips (Sigma Millipore ...
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bioRxiv - Immunology 2023Quote: Samples were analyzed by high resolution/high accuracy LC-MS/MS (Lumos Fusion, Thermo Fisher). Peptides were separated using direct loading onto a packed-in-emitter C18 column (75um ID/12cm ...
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bioRxiv - Genomics 2023Quote: ... were quantified using Qubit dsDNA High Sensitivity and RNA High Sensitivity Assay kits (ThermoFisher Scientific) and stored in aliquots at -20°C.
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bioRxiv - Microbiology 2020Quote: ... we used human monoclonal antibodies produced recombinantly in human Expi293F cells (Life Technologies) as described before (Fang et al ...
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bioRxiv - Cell Biology 2021Quote: ... anti-human IgG-Alexa647 and anti-human IgG-Alexa568 were obtained from Invitrogen, anti-acetylated tubulin (T7451 ...
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bioRxiv - Cell Biology 2020Quote: ... and human GAPDH and human KIF18A Taqman probes and primers (Thermo Fisher Scientific) were used for reverse transcription and qRT-PCR ...
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bioRxiv - Neuroscience 2020Quote: ... and subjected to human total tau ELISA (human tau: # KHB0042, Thermo Fisher Scientific) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with high glucose (1:1 v/v, Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10 % fetal bovine serum ...
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bioRxiv - Physiology 2023Quote: ... cells were resuspended in plating medium (DMEM high glucose #1-26P02-K, M199 EBS #1-21F01-I; both from BioConcept, 10% horse serum (#16050130; Thermo Fisher Scientific), 5% fetal calf serum (#10270-106 ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mL min-1) and identity of peptides confirmed by high-resolution mass (Q Exactive Orbitrap LC-MS/MS (Thermo Fisher Scientific).
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bioRxiv - Genomics 2023Quote: ... coated dishes in N2 medium (high-glucose DMEM, 1% N-2, 2% B-27 and 1% penicillin/streptomycin, from Thermo Fisher Scientific) at a density of 2×105 cells/cm2 ...
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bioRxiv - Molecular Biology 2021Quote: ... slides were washed thrice for 5 min in PBS before incubation with Alexa 594 goat-anti rabbit secondary antibody at 1:1,000 and Alexa Fluor 488 goat anti-human IgG(H+L) at 1:500 (both Invitrogen; in PBS) for 2 h at RT ...
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bioRxiv - Cell Biology 2021Quote: ... EpiLCs were induced by plating 1.5 x 106 ESCs on a 10 cm dish coated with human plasma fibronectin (16.7 mg/ml) in the 1:1 mix of DMEM/F12 (Gibco, 11302-33) and Neurobasal medium containing N2 supplements ...
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bioRxiv - Evolutionary Biology 2020Quote: ... a formulation optimal for human fibroblasts [(Dickson et al., 2000): 1:1 (vol/vol) medium M199 and medium M106 (ThermoFisher Scientific) + 15% BCS + 10 ng/ml EGF + 0.4 ug/ml hydrocortisone + 1% Penicillin-Streptomycin] ...
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bioRxiv - Biophysics 2024Quote: ... primary stained with goat anti-Syndecan-1 antibody and mouse anti-human choriogonadotropin (β-hCG) antibody (#14-6508-82, 1:500 dilution in the buffer solution, ThermoFisher), and secondary stained as previously described ...
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bioRxiv - Microbiology 2023Quote: ... and was then incubated (1:1000 dilution) for 1 hour with a mouse anti-human E-Cad ectodomain antibody (HECD-131700, 1:1000 dilution; Life Technologies). After two washes ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies used in this study were all anti-human: STING (1:1000, CST, #13647 or 1:500, Thermo Fisher, MA526030), UBIQUITIN (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 h at room temperature and then exposed to antibodies specific for human PD-1 (ThermoFisher Scientific; 14-9969-82), Iba1 (Wako Chemicals ...
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bioRxiv - Bioengineering 2023Quote: ... primary T cells were activated and stimulated with a 1:1 ratio of anti-human CD3/CD28 magnetic Dynabeads (Thermo Fisher) to cells in X-vivo 15 medium supplemented with 5% human AB serum (Heat inactivated) ...
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bioRxiv - Biophysics 2020Quote: ... the head group-labeled fluorescent dye Texas Red 1,2-dihexadecanoyl 3-phosphoethanolamine (TR-DHPE) (Molecular Probes, Eugene, OR) was first dissolved in chloroform and subsequently added to lipid spreading solutions at 0.5 mol% ...
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bioRxiv - Immunology 2021Quote: Groups of 10 embryos were lysed and homogenised using a 27-gauge needle in 500 μl Trizol (Invitrogen) for RNA extraction ...
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bioRxiv - Cell Biology 2021Quote: ... the viral supernatants from the test groups were added into the deep well plate (KingFisher™Thermo Scientific) along with a lysis buffer containing the following components - 260 μL ...
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Therapeutic base and prime editing of COL7A1 mutations in recessive dystrophic epidermolysis bullosabioRxiv - Bioengineering 2021Quote: ... equal amounts of proteins from each group were loaded onto Nupage Novex Bis-Tris Gels (Thermo Fisher Scientific), and electrophoresis was performed using an X-cell SureLock Mini-Cell (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2021Quote: ... Endotoxin contamination was assessed for all NP groups using Pierce™ LAL Chromogenic Endotoxin Quantitation Kit (ThermoFisher Scientific) according to manufacturer’s guidelines.
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bioRxiv - Bioengineering 2020Quote: ... PBMCs were seeded on top of cell-free GelMA discs (n = 5 per group) with a density of 2.5 ⨯ 105/ml and cultured in 0.5 ml RPMI-1640 + GlutaMAX (Gibco) supplemented with 10% FBS and 1% P/S for 24 hours in 48-wells plates ...
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bioRxiv - Neuroscience 2021Quote: The HEK293T cells were divided into four groups and transfected with plasmids by Lipofectamine 2000 (11668-027, Invitrogen). The four groups (tTAN ...
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bioRxiv - Genomics 2021Quote: ... 2 × 105 cells from each group were maintained in erythroid differentiation media (IMDM media (Fisher Scientific, Hampton, NH) containing 1 ng/ml hIL-3 ...
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bioRxiv - Immunology 2022Quote: Two groups of PBMCs (5×106 cells/ml) were stimulated with 1μg/ml anti-CD3/CD28 antibodies (Invitrogen) at 37°C under 5% CO2 for 18 hours in RPMI-1640 medium ...
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bioRxiv - Genetics 2020Quote: mRNA was collected from groups of 10 whole larvae (n=3–4 replicates per line) using Trizol (ThermoFisher) and reverse transcribed to cDNA using SuperScript III Reverse Transcriptase (ThermoFisher) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Samples of the visceral yolk sac (n=6/group) were immersed in RNA Later solution (Invitrogen, MA, USA) and frozen at −20°C until further processing ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was extracted from larvae (groups of 10) using TRIzol according to manufacturer’s instructions (Invitrogen; 15596-018). RNA samples were then subjected to DNase treatment according to manufacturer’s instructions (Ambion ...
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bioRxiv - Microbiology 2021Quote: ... The viral supernatants from the test groups were added into the deep well plate (KingFisher™ Thermo Scientific) along with a lysis buffer containing the following components - MagMAX™ Viral/Pathogen Binding Solution ...
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bioRxiv - Immunology 2020Quote: Groups of 10 embryos were lysed and homogenised using a 27-gauge needle in 500 μl Trizol (Invitrogen) and RNA extracted as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Isolated cells pooled from multiple mice per group were processed using an Invitrogen mirVana miRNA Isolation kit (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 484 bp for group 2) was excised using a 2% E-Gel SizeSelect Agarose Gels (Thermo Fisher Scientific). The sequence library was adjusted to 10 pM (assuming 1 bp DNA has a molecular weight of 660 g/mol ...
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bioRxiv - Evolutionary Biology 2024Quote: Scans for all 48 species were segmented in Amira 3D (version 2021.1, Visualization Science Group, Thermo Fisher Scientific) and the digital models outputted were cleaned and further processed in MeshLab (28) ...
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bioRxiv - Cell Biology 2023Quote: ... All parasite cultures were maintained in blood group O RBCs at 4% hematocrit in RPMI-1640 medium (Gibco) containing 27 nM NaHCO3 (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... 3000 Plp1+ cells from each group (control, D1, D3, D7) were collected into 96-well plates (Life Technologies) coated with BSA (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: RNAs (10-50 μM) with a 5’ terminal hydroxyl group were incubated with T4 polynucleotide kinase (Thermo Scientific) and 𝛾-32P-ATP (10 mCi/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... Epifluorescent images were captured using an EVOS FL inverted microscope by American Microscope Group (AMG) (Thermo Fisher Scientific) with a 40X oil objective and GFP ...