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Citations for Thermo Fisher :
2451 - 2500 of 10000+ citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade D UG28R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... Then chromatin was pre-cleared with 10 μl of 1:1 protein A:protein G Dynabeads (Thermo Fisher Scientific, 10001D and 10003D). The precleared chromatin sample was incubated with 0.5 μg of anti-H3K4me3 (Millipore 17-614 ...
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bioRxiv - Genomics 2023Quote: The MNase digested chromatin solution of each sample was pre-cleared with 100μl Dynabeads Protein A/G 1:1 mix magnetic beads (Invitrogen 10001D, 10003D) and rotated at 4℃ for 2h ...
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bioRxiv - Immunology 2024Quote: ... or LAMP-1/CD107a monoclonal antibody specific for the lysosomal-associated membrane protein 1 (LAMP-1) of the parasitophorous vacuole (rat IgG2a FITC, Invitrogen, CA). Revelation was performed with 1.5 μg/ml streptavidin conjugated to Texas Red (Molecular Probes ...
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bioRxiv - Microbiology 2021Quote: ... Induction of genes on plasmids was accomplished by the addition of 0.5 mM Isopropyl β- D-1-thiogalactopyranoside (IPTG) (Thermo Fisher), 0.2% arabinose (Sigma) ...
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bioRxiv - Molecular Biology 2021Quote: ... The beads were washed five times with lysis buffer containing 1 M NaCl and incubated with 25 µl of biotin elution buffer (lysis buffer with 5 mM D-biotin [Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... and cultured in minimal medium containing 1 part of DMEM (4.5 g/L D-Glucose, Pyruvate, L-Glutamine; Life Technologies; 41966029): 1 part DMEM/F12 (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... Additional primary antibodies used in this study for the brain were: mouse anti-HuC/D (1:200, Thermo Fisher Scientific, #A21271), mouse anti-NeuN (1:200 ...
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bioRxiv - Immunology 2022Quote: Total RNA was prepared from one lung lobe collected at 3 dpi using lysing matrix D (MP Biomedical) containing 1 ml of TRIzol reagent (Ambion, Life technologies) and homogenization at 20 s at 4.0 m/s twice using MP Biomedical Fastprep 24 Tissue Homogenizer ...
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bioRxiv - Microbiology 2022Quote: ... 1 μg of RNA was reverse transcribed using SuperScript II Reverse Transcriptase with oligo d(T) primers (cat#12574026, Thermofisher Scientific), and cDNA was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR ...
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bioRxiv - Cell Biology 2020Quote: ... Eggs were washed extensively in D-PBSTB and incubated with secondary antibodies for 2h at RT - donkey anti-rabbit Alexa 555 (dilution 1:1000; Molecular Probes), goat anti-rat Alexa 488 (dilution 1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... cells either stably expressing eGFP or NOSTIC-1 were seeded on poly-D-lysine coated cover slips (Thermo Fisher, Waltham, MA). When these cells were about 60% confluent ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated on ice for 15 min and induction was initiated with the addition of 0.4 mM isopropyl ≥-D-1-thiogalactopyranoside (IPTG) (Fisher Scientific; BP1755). Proteins were produced for 16-18 hr at 18°C.
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bioRxiv - Cell Biology 2022Quote: ... coding sequences of CMV MPs and CmVPS41s were PCR amplified using specific primers (Supplemental table 1) and cloned into plasmid pENTR/D-TOPO (Thermo Scientific by Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... coding sequence were PCR-amplified from maize leaf cDNA with the appropriate primers (Supplementary Table 1) and cloned into the pENTR/D-TOPO entry vector (Thermo Scientific). Clones were recombined with the vectors pSITE-2CA and pSITE- 1NB using LR Clonase Mix II (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... that had been coated overnight with 100 μg/ mL poly-D-lysine at 37 °C and for 1 h at 37 °C with 10 μg/mL laminin (Life Technologies). After 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... were maintained in DMEM with 1g/L of D-glucose supplemented with 8% Fetal Bovine Serum/FBS and 1% Pen/Strip (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: The expression of NPP-19 [1-301] fragment N-terminally fused to a 6xHis tag was induced by the addition of 1 mM of isopropyl β-D-thiogalactopyranoside (IPTG) to 1 L Luria Broth cultures of exponentially growing Escherichia coli BL21 DE3 pLysS (Invitrogen) strain (OD = 0.6) ...
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bioRxiv - Cell Biology 2023Quote: The expression of NPP-10N N-terminally fused to MBP was induced by the addition of 1 mM of isopropyl β-D-thiogalactopyranoside (IPTG) to 1 L Luria Broth cultures of exponentially growing Escherichia coli BL21 DE3 pLysS (Invitrogen) strain (OD = 0.6) ...
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bioRxiv - Cell Biology 2023Quote: The expression of NPP-19 full length N-terminally fused to GST was induced by the addition of 1 mM of isopropyl β-D-thiogalactopyranoside (IPTG) to 1 L Luria Broth cultures of exponentially growing Escherichia coli BL21 DE3 pLysS (Invitrogen) strain (OD = 0.6) ...
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bioRxiv - Neuroscience 2023Quote: ... into low passage HEK-293T cells plated at 1×106 cells/well of a poly-D-lysine-coated 6-well plate using Lipofectamine 3000 (Life Technologies). The next day ...
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bioRxiv - Immunology 2024Quote: ... washed and 1×105 cells in 50 µL of ATP assay media were plated per well on a Poly-D-lysine (Gibco, A3890401) coated XF96 microplate (Agilent ...
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bioRxiv - Neuroscience 2024Quote: ... slides were subsequently incubated in mouse monoclonal antiserum against the neuronal marker HuC/D (1:2500; ThermoFisher Scientific; A-21271; AB_221448) followed by Cy3-conjugated donkey anti-mouse IgG (1:500 ...
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bioRxiv - Cell Biology 2019Quote: ... The samples were then rinsed with PBS (5 × 3 min) and incubated for 1 h at 37°C with the following secondary antibodies: anti-human Alexa 488 (Thermo Fisher, Waltham, MA, USA; 1:750) or anti-mouse Alexa 488 (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations of the subcellular protein fraction lysates were measured using Pierce BCA Protein Assay Kit (ThermoFisher). Samples were boiled for 10 minutes and the extracts were separated by SDS-polyacrylamide gel electrophoresis using precast 4–20% Precise protein gels (ThermoScientific) ...
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bioRxiv - Neuroscience 2020Quote: ... Rab27b protein levels were normalized to total protein levels determined by SYPRO Ruby protein gel stain (Invitrogen).
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bioRxiv - Cancer Biology 2021Quote: The protein–protein interaction for endogenous protein was studied by using the Co-Immunoprecipitation Kit (Thermo Scientific). For overexpressed HA-tag proteins ...
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bioRxiv - Neuroscience 2023Quote: ... Synaptosomal protein expression levels were normalized to total protein using No-Stain Protein Labeling Reagent (Invitrogen, A44449). Primary antibodies (Anti-Shank3 (N367/62) ...
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bioRxiv - Microbiology 2023Quote: ... antibody-protein complexes were deposited on 25µL of protein A magnetic beads (Dynabeads protein A, Life Technologies) and incubated for 4h at 4°C on the rotator ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were incubated with Protein-A beads (Thermo Fisher Scientific) conjugated with anti-p53 antibody or Mouse IgG at 4 °C overnight with gentle shaking ...
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bioRxiv - Molecular Biology 2019Quote: ... protein was quantified using the Qubit Protein Assay Kit (Invitrogen). Antibodies used were ...
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bioRxiv - Immunology 2021Quote: ... Total protein was measured by Pierce™ Protein Assay (ThermoFisher). Cytokine levels were standardized to total protein content.
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bioRxiv - Microbiology 2019Quote: ... Protein concentration was determined using BCA protein Assay (Thermo Scientific).
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bioRxiv - Neuroscience 2020Quote: ... Protein concentration was determined using a BCA protein assay (ThermoFisher). Samples were then reduced in Laemmli buffer and incubated for 5 min at 95°C ...
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bioRxiv - Cell Biology 2022Quote: ... Total protein quantified using BCA Protein Assay Kit (Thermo Fisher) with bovine serum albumin as a protein standard ...
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bioRxiv - Immunology 2022Quote: ... Protein concentration was determined using EZQ protein quantification kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... Protein concentrations were determined by BCA protein assay (Thermo Fisher) using BSA as a standard ...
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bioRxiv - Developmental Biology 2019Quote: ... Protein concentration was measured via Pierce BCA protein assay (ThermoFisher). Approximately 15 μg supernatant protein was diluted 4:1 in reducing 4X Laemmeli buffer for 1 hour at room temperature and separated at 100V for 1 hour on 10% SDS-PAGE gels ...
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bioRxiv - Neuroscience 2020Quote: ... Protein sample concentrations were determined using Bradford protein assay (ThermoFisher). Protein samples were prepared with 4x LDS (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein concentration was assessed by BCA protein assay (Thermo Scientific). To ensure that equal amount of protein undergoes RBD pulldown ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein concentration was assessed by BCA protein assay (Thermo Scientific).
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bioRxiv - Neuroscience 2020Quote: ... Proteins were coimmunoprecipitated using Protein A Dynabeads (Thermo Fisher Scientific) and Western blots were performed as described above (1:1000 used for both anti-Ryk and anti-Pcdh19 primary antibodies for Western blots).
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bioRxiv - Genetics 2021Quote: ... Protein concentration was measured using BCA Protein Assays (Thermo Fisher). Samples were then digested overnight with rLys-C (Promega) ...
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bioRxiv - Physiology 2021Quote: After determining myocardial protein concentrations (Pierce BCA Protein Assay; ThermoFisher) protein aggregate accrual in the heart was measured using a commercially available kit (Proteostat ...
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bioRxiv - Molecular Biology 2021Quote: ... the protein was quantified with Qubit Protein assay (Life Technologies) and Pierce BCA Protein Assay Kit (Thermo Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were estimated using the BCA protein estimation kit (ThermoFisher) and subsequently separated by 10% SDS-PAGE and transferred onto nitrocellulose membrane (Pall Corporation ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentrations were measured by BCA protein assay kit (ThermoFisher), equal amounts of proteins were analyzed for western blotting ...
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bioRxiv - Microbiology 2021Quote: ... Protein concentration was determined by BCA protein assay (ThermoFisher Scientific) and 20 µg total protein was loaded ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was quantitated with BCA Protein Assay (Thermofisher Scientific) and stored in aliquots at −80 °C until used.
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bioRxiv - Cancer Biology 2020Quote: ... coupled with protein A and protein G beads (Life Technologies) at 4 degrees Celsius overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... Equivalent protein concentrations were immunoprecipitated with Protein G DynabeadsTM (Invitrogen) coupled to a mouse anti-biotin primary antibody ...