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Citations for Thermo Fisher :
2201 - 2250 of 10000+ citations for IL 1beta Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... NIKWT and NIKKO BMDMs were stimulated with IL-4 (20ng/mL) for 24 hours before lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: 1 × 106 NIKWT and NIKKO BMDMs were stimulated with 20ng/mL IL-4 or PBS for 6 hours before being lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... T cells were expanded in RPMI-1640 media supplemented with 10% heat-inactivated FBS (HI-FBS) as well as recombinant human IL-2 (50 U/mL; ThermoFisher) and IL-15 (1 ng/mL ...
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bioRxiv - Immunology 2022Quote: IFNγ mRNA and protein were measured in IL-2 expanded MAIT cells (with or without stimulation with CD3/CD28 Dynabeads (Gibco)) and their culture supernatant by qPCR or ELISA respectively ...
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bioRxiv - Immunology 2022Quote: ... and activated in complete RPMI supplemented with 30 U/mL IL-2 with anti-human CD3/CD28 dynabeads (11131D, ThermoFisher) at a 1:1 cell-to-bead ratio ...
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bioRxiv - Evolutionary Biology 2023Quote: ... protein concentration in the supernatant was determined using the Coomassie (Bradford) Protein assay Kit (Thermo Fisher Scientific, Rockford, IL, USA) and bovine serum albumin (BSA ...
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bioRxiv - Evolutionary Biology 2023Quote: ... protein concentration of the CF was determined using the Coomassie (Bradford) Protein assay Kit (Thermo Fisher Scientific, Rockford, IL, USA) and bovine serum albumin (BSA ...
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bioRxiv - Immunology 2023Quote: ... using permeabilization buffer (eBioscience) and stained with the following cytokine antibodies for 30 min at 4°C: IL-17A (eBio17B7, FITC, Invitrogen), IFN-ψ (XMG1.2 ...
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bioRxiv - Biophysics 2023Quote: ... the total protein concentration was determined using the bicinchoninic acid (BCA) protein assay kit (Pierce/Thermo Fisher Scientific; Rockford, IL). 25µg of protein samples were mixed with 4x LDS sample buffer (Life Technologies ...
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bioRxiv - Immunology 2024Quote: Cell culture supernatants collected at various time intervals from in vitro stimulated T cells of mice or humans were analyzed for IL-2 by ELISA using kits purchased from ThermoFisher Scientific (mouse IL-2 ELISA kit ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... The membranes were then treated with secondary antibodies (biotinylated goat anti-rabbit IgG (#65-6140, Invitrogen, Rockford, IL, United States), biotinylated goat anti-rat IgG (#405428 ...
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bioRxiv - Immunology 2024Quote: ... Untreated 96 flat bottom plates were coated overnight at 4°C with capture antibody for IFNγ or IL-17A from Invitrogen kits (Cat# 88-7314-22 and Cat# 88-7371-88 respectively from Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... Gene expression levels were measured by quantitative real-time PCR using the SYBR Green assay (Thermo Scientific, Rockford, IL, USA)on a StepOne instrument (Thermo Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... in a final volume of 10□µl using an ABI Prism 7000 Sequence Detection System (Thermo Scientific, Rockford, IL, USA). The PCR reactions were initiated with a 10-min incubation at 95□°C followed by 40 cycles of 95□°C for 15□s and 60□°C for 60□s ...
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bioRxiv - Immunology 2024Quote: ... Cell culture supernatants were collected at the end of culture and analyzed for IL-2 by ELISA using a kit (cat # 88-7025-76) purchased from Thermofisher Scientific.
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bioRxiv - Microbiology 2024Quote: ... The proteins were digested into peptides by adding 0.95 µg of MS grade trypsin (Thermo Scientific Pierce, Rockford, IL, USA) solubilized in 40 µl of ABC buffer to the filters and incubating for 16 hours in a wet chamber at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein concentration of the supernatant for immunoprecipitation was measured via the bicinchoninic acid assay (Thermo Scientific, Rockford, IL, USA) (Walker ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse IgGs were stained with Alexa Fluor 568 conjugated donkey anti-mouse IgG (Invitrogen, Cat#A-10037). Panleucocytes were identified using rat anti-mouse CD45 antibodies (BD Pharmingen Inc ...
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bioRxiv - Neuroscience 2021Quote: ... biotinylated horse anti-mouse IgG (H+L) and biotinylated goat anti-mouse IgG2a (Life Technologies, ThermoFisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... biotinylated horse anti-mouse IgG (H+L) and biotinylated goat anti-mouse IgG2a (Life Technologies, ThermoFisher Scientific).
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bioRxiv - Bioengineering 2022Quote: ... Mouse IgG2a Fc Tag (Acro Biosystems) incubation followed with APC Goat anti-mouse IgG2a Fc Antibody (Invitrogen) staining and RFP expression ...
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bioRxiv - Molecular Biology 2020Quote: ... and mouse antibodies were coupled to Dynabeads coated with Pan Mouse IgG antibodies (Thermo Fisher Scientific, 11042). 50 μL of the appropriate Dynabeads pre-coupled with the indicated antibody were added to the chromatin sample and incubated overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... goat anti-mouse Alexa Fluor 568 or goat anti-mouse Alexa Fluor 488 secondary antibody (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... Mouse monoclonal antibodies were detected with a goat anti-mouse IgG HRP-linked antibody (62-6520; Invitrogen) diluted 1:10,000 (v/v) ...
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bioRxiv - Immunology 2020Quote: ... Isotype control and mouse monoclonal antibody to mouse IFNβ (Clone: MAR1-5A3) were purchased from Thermo scientific. SP600125 and C-16 purchased from Calbiochem ...
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bioRxiv - Microbiology 2022Quote: ... CA) or Alexa Fluor 488 anti-mouse antibody (for LP11 and mouse anti-myc; Invitrogen, Waltham, MA) at a 1:250 dilution in FACS medium ...
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bioRxiv - Neuroscience 2024Quote: ... BV-2 cells were fixed and sequentially incubated with Mouse-on-Mouse IgG Blocking Solution (Invitrogen R37621) and Navenci blocking solution at 37°C for 60 min each in a pre-heated humidified chamber ...
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bioRxiv - Cancer Biology 2023Quote: ... Mouse IL21 protein levels in BM supernatant were determined using an IL21 Mouse ELISA kit (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... Alexa Fluor 488 goat anti-mouse and anti-rabbit IgG (Invitrogen, Waltham, MA; catalog # A-11001 (mouse) and A-11008 (rabbit) ...
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bioRxiv - Neuroscience 2023Quote: Mouse samples: total RNA from mouse blood samples was extracted using the RiboPure RNA Purification Kit (Invitrogen). Total RNA from brain regions was extracted ...
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bioRxiv - Neuroscience 2024Quote: ... and 647 (1:500 for all; PIA32773-mouse; A21202-mouse; PIA32731TR-rabbit; PIA32794-rabbit; A21448-sheep, Invitrogen). The sections were examined using an AX-R Confocal Microscope coupled to a fully automated Ti2-E system (Nikon Instruments Inc ...
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bioRxiv - Microbiology 2024Quote: ... and amastigotes were labeled with mouse anti-P8 and A488-conjugated goat anti-mouse secondary antibody (Invitrogen); samples were co-stained with DAPI ...
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bioRxiv - Microbiology 2024Quote: ... and amastigotes were relabeled with mouse anti-P8 and A488-conjugated goat anti-mouse secondary antibody (Invitrogen); samples were co-stained with DAPI ...
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bioRxiv - Molecular Biology 2021Quote: ... Primary (anti-flag mouse 1:200, Cell Signalling) and secondary antibody (anti-mouse Alexa 488 1:500, Thermofisher) were diluted in blocking buffer solution and incubated overnight at 4°C or 1 hour at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were incubated with secondary anti-mouse antibody conjugated to HRP (goat anti-mouse-HRP, 32430; ThermoFisher Scientific) at 1:20,000 in 5% milk/1xTBST for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... or with unconjugated 4.3.1.3 mouse MAb and secondary detection with goat anti-mouse Alexa-Fluor 647 (A-21235; Life Technologies). Immunofluorescence was used to boost GFP signal in TgBAC(lck:gfp)vcc6 specimens with a chicken anti-GFP primary (Abcam ...
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bioRxiv - Neuroscience 2022Quote: Total mouse tau ELISA measurements were carried out using a total mouse tau ELISA kit (Thermo Fisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2019Quote: Cells were obtained from mouse spinal cord and/or cultured mouse embryonic fibroblasts resuspended in Trizol (Thermo Fisher). RNA was extracted using miRNeasy Mini Kit (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... mouse anti-human IgG3 (HP6050) or mouse anti-human IgM (clone HP6083) at 1/1000 (Thermo Fisher Scientific) for 1 hour at RT ...
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bioRxiv - Genomics 2022Quote: ... anti-mouse 800 (only used in Figure 8E,F to detect mouse anti- FLAG) (1:5000, Invitrogen A32730). Anti-Sloth1 and Anti-Sloth2 antibodies (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... and with LIN (anti-mouse TER119-FITC, Thermo Fisher, 11-5921-82; anti-mouse CD31-FITC, Thermo Fisher, 11-0311-85 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Total RNA was extracted from mouse skin tissue or mouse/human EpSCs using Trizol reagent (Invitrogen, Massachusetts, USA), and used as a template for cDNA synthesize using HiScript II reverse transcriptase (Vazyme Biotech ...
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bioRxiv - Immunology 2024Quote: CLP was performed on WT and Plk3−/− mice following retro orbital injection (0.1μg/g mouse) of anti-mouse GPIX (Emfret) conjugated to AlexaFluor-750 (ThermoFisher) as previously described 21 ...
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bioRxiv - Neuroscience 2023Quote: ... goat anti-mouse IgG Alexa546-labeled monoclonal antibody goat anti-mouse IgG Alexa633-labeled monoclonal antibody (ThermoFisher Scientific); DAPI ...
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bioRxiv - Immunology 2023Quote: ... The secondary antibody reactive to mouse IgG (anti-Mouse IgG (H+L)-AF647) was purchased from Thermo Fisher Scientific.
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bioRxiv - Microbiology 2023Quote: ... mouse primary antibody was visualised with 1:1000 Donkey α Mouse Alexa Fluor™ Plus 647 (Invitrogen, A32787) or 1:1000 Donkey α Mouse Alexa Fluor™ Plus 555 (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... mouse anti-human IgG3 (HP6050) or mouse anti-human IgM (clone HP6083) at 1/1000 (Thermo Fisher Scientific) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... or RGS14 (n=3, 1:500, mouse anti-RGS14 #75-170, Neuromab)/goat anti-mouse IgG2a (Life Technologies).
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bioRxiv - Cancer Biology 2021Quote: ... mouse anti-KRT 14 (1:10, Invitrogen); rabbit anti-KRT 19 (1:10 ...
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bioRxiv - Cancer Biology 2021Quote: ... and Mouse anti-CD31 (ThermoFisher; 1:100). Secondary antibodies were Goat and Donkey anti-IgG and usually either Alexa Fluor-488 ...