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Citations for Thermo Fisher :
2001 - 2050 of 10000+ citations for IL 1beta Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... mouse anti-paxillin (MA5-13356, Thermofisher), mouse anti-vimentin (ab8069 ...
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bioRxiv - Biophysics 2021Quote: ... Subcultures were grown at a 1:10 ratio of subculture aliquot to Middlebrook 7H9 media (Fisher Scientific, Hanover Park, IL) until an OD of approximately 0.5 was attained ...
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bioRxiv - Biophysics 2021Quote: ... Total protein concentration in the BAL fluid was measured using the Pierce™ BCA assay kit (Thermo Scientific, Rockford, IL), as previously described.6
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bioRxiv - Cancer Biology 2021Quote: ... a drop of ProLong Gold Antifade reagent with Dapi was added to each section (Thermo Fisher Scientific, Rockford, IL, USA). Images of these slides were compiled and analyzed in ImageJ.
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bioRxiv - Immunology 2021Quote: ... mice were administered 150 μg of anti-IL-13 (eBio1316H cat# 16-7135-85) or IgG (eBRG1 cat# 16430185) from Thermofisher. For anti-CCR2 cell depleting treatment ...
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bioRxiv - Immunology 2021Quote: Enzyme-linked immunosorbent assay (ELISA) was performed for IFNγ and IL-2 using uncoated kits (respectively 88-7316-88 and 88-7025-88, ThermoFisher) following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Concentrations of IFN-λ1 in basolateral medium samples from WD-PNECs were measured using a human IFN-λ1/IL-29 ELISA (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and IL-1β in all of samples were determined using the appropriate ELISA kits (Thermo Fisher Scientific, Waltham, MA, USA), according to the manufactures’ protocols ...
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bioRxiv - Bioengineering 2020Quote: ... All T cells were expanded in complete T-cell medium and fed interleukin (IL)-2 (50 U/mL; Life Technologies) and IL-15 (1 ng/mL ...
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bioRxiv - Immunology 2021Quote: Supernatants from MSC and T-cell cocultures were analyzed using IL-10 Ready SET-Go! ELISA kits kits (Affymetrix, eBioscience) for secretion of IL-10 as previously described [31] ...
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bioRxiv - Plant Biology 2020Quote: ... Isobaric labeling of the samples was performed using 10-plex tandem mass tag (TMT) reagents (Thermo Fisher Scientific, Rockford, IL). All individual and pooled samples were labeled according to the TMT 10-plex reagent kit instructions ...
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bioRxiv - Microbiology 2021Quote: ... Supernatants were assayed for IL-2 or IFNγ by enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s instructions (Thermo fisher).
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bioRxiv - Bioengineering 2021Quote: ... IL-8 concentrations in medium samples from the basal compartment were measured using the IL-8 Human Uncoated ELISA Kit (88-8086-88, Invitrogen). Assays were performed in Nunc MaxiSorp (tm ...
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bioRxiv - Bioengineering 2020Quote: The supernatants were collected after cytotoxicity assay for cytokine analysis by using IFN-γ and IL-2 ELISA kits (Invitrogen). Cytokine levels were analyzed by comparing the values obtained from samples to a standard curve generated from known concentrations of the cytokines.
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bioRxiv - Bioengineering 2020Quote: ... and media for M(IL4/IL13) stimulation consisted of RPMI 1640 with 20 ng/ml interleukin 4 (IL-4; Invitrogen) and 20 ng/ml interleukin 13 (IL-13 ...
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bioRxiv - Bioengineering 2020Quote: ... T cells were cultured in RPMI-1640 supplemented with 10% HI-FBS and fed with recombinant human IL-2 (ThermoFisher) and IL-15 (Miltenyi ...
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bioRxiv - Cancer Biology 2020Quote: ... Supernatant of cell lysates was assayed for total protein content using the BCA protein assay (Thermo Fisher Scientific, Rockford, IL), and 70 μg of total protein per sample was separated in a 7.5% polyacrylamide gel (Invitrogen) ...
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bioRxiv - Cancer Biology 2019Quote: ... for IL-6) for 2 hours at RT and followed by incubating in 1:1000 DAPI (62248, Thermo Fisher Scientific) in PBS for 1min ...
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bioRxiv - Systems Biology 2021Quote: ... Serum TNFα and IL-1β concentrations were assayed using commercial ELISA kits according to the manufacturer’s instructions (ThermoFisher Scientific, UK).
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bioRxiv - Molecular Biology 2020Quote: ... all the enzymes were buffer exchanged against 1 mol·L−1 ammonium acetate at pH 7.5 using microcentrifuge gel-filtration columns (Zeba 0.5 mL, Thermo Scientific, Rockford, IL). Protein concentration was determined with a NanoDrop™ spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2021Quote: ... Isolated RNA concentration was measured and RNA quality was tested by NanoDrop-1000 spectrophotometer (Thermo Fisher Scientific, Vernon Hills, IL). Two micrograms of RNA were used for cDNA transcription (Applied Biosystems ...
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bioRxiv - Cell Biology 2021Quote: Serial skeletal muscle cross sections (7μm) were sectioned onto room temperature uncoated glass slides (ThermoFisher SuperFrost+, Fisher Scientific, Rockford, IL) and air-dried to remove excess water ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained (10 μL/1.5×105 cells) with anti-IL-1 alpha (Thermo Fisher Scientific; Cat.# 11-7118-81) and anti IgG1 kappa (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... embedded in OCT and stored at −80C until 16 μm cryostat sections were collected (Lecia, Buffalo Grove, IL) onto Superfrost plus glass slides (Invitrogen). For both tissues and cells ...
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bioRxiv - Cancer Biology 2021Quote: ... Supernatant was collected for 7 days and the antibody was purified using a protein A column (ThermoFisher Scientific, 89960; IL). The original murine anti-PD1 monoclonal antibody (63 ...
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bioRxiv - Neuroscience 2022Quote: ... Serum TNF and IL-1β concentrations were determined by using a rat TNF alpha Uncoated ELISA and a rat IL-1β ELISA Kit (all from Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... were assessed using the following ELISA kits per manufacturer protocols: (IgG; Euroimmun - EI2606-9601G, IgA; Euroimmun - EI2606-9601A, IL-6; Invitrogen - 88-7066.22 ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were washed and incubated in a DNA staining solution (1:10,000 Hoechst 33342, Thermo Scientific, Rockford, IL, USA) for 3-6 minutes ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T helper cell subsets were identified using the antibodies listed above and the addition of anti-IL-5 (for Th2) and anti-IFNγ (for Th1) after permeabilization (Invitrogen). Gates were set according to Fluorescence Minus One (FMO ...
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bioRxiv - Microbiology 2019Quote: ... the complexes were encapsulated by adding 15 µl of protein transfection reagent (PTR) Pro-Ject™ (Thermo Scientific, Rockford, IL). The sample was mixed by pipetting and then incubated for 30 min ...
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bioRxiv - Microbiology 2019Quote: ... The protein concentrations of the lysates were then quantified using a bicinchoninic acid protein kit (Thermo Fisher Scientific, Rockford, IL). Aliquots containing 12 µg of total cellular lysates for each sample were denatured at 100 °C for 5 min and separated using 8% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Physiology 2019Quote: ... The protein concentration was measured with the reducing agent compatible Pierce BCA Protein Assay Kit (Thermo Scientific, Rockford, IL, USA).
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bioRxiv - Immunology 2019Quote: ... and IL-18 were determined using the Platinum ELISA kit for the quantitative detection of the three cytokines (ThermoFisher SCIENTIFIC Invitrogen, Vienna ...
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bioRxiv - Cell Biology 2019Quote: ... The colorimetric reaction was then measured at 540 nm using a Multiskan Go microplate reader (Thermo Scientific, Rockford, IL, USA).
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bioRxiv - Immunology 2020Quote: ... IL-1β ELISA kit (Catalog # 88-7013-22) and TNFα ELISA kit (Catalog # 88-7324-88) were from Invitrogen (America). LDH Cytotoxicity Assay Kit (C00170 ...
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bioRxiv - Cancer Biology 2019Quote: The human myeloma cell line KJON (19) was cultured in RPMI with 5 % heat inactivated human serum (HS) and 2 ng/mL interleukin (IL)-6 (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Pathology 2020Quote: ... cells were first synchronized overnight in 1% FCS DMEM and then cultured for 14 days in 10% FCS DMEM and stimulated daily with IL-1β (1 ng/ml; Invitrogen) or IL-17 at (2.5 ng/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... The cell culture supernatant was collected and used for quantifying IL-8 secretion using the ELISA assay kit (# 88-8086-88, ThermoFisher). The assay was performed following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... The tissue was homogenized for 4 sec twice on ice using a PowerGen 125 grinder (Fisher Scientific, Rockford, IL, USA). The homogenate was transferred to a fresh 2 mL microcentrifuge tube ...
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bioRxiv - Neuroscience 2019Quote: ... anti-rabbit IgG-Alexa Fluor 488 (R37116, RRID:AB_2556544) and anti-chicken IgG-Alexa Fluor 594 (A11042, RRID:AB_142803) (Thermo Fisher Scientific, Rockford, IL).
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bioRxiv - Immunology 2021Quote: - IFNγ and IL-10 levels were measured in the supernatant of the WBA using IFN gamma and IL-10 Human ELISA Kit (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2021Quote: ... and protein disulfide bonds were reduced using 10mM dithiothreitol and alkylated with 25mM iodoacetamide (Thermo Fisher Scientific, Rockford, IL, USA). Proteins were then deglycosylated with PNGaseF (New England Biolabs ...
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bioRxiv - Physiology 2021Quote: ... Peptide concentration was measured with a colorimetric assay (Pierce Quantitative Colorimetric Peptide Assay kit, Thermo Fisher Scientific, Rockford, IL, USA).
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bioRxiv - Physiology 2021Quote: ... and the Y2H library screening was performed using the GAL4-base system following the manufacturer’s instruction (Thermo Scientific, Rockford, IL). The bait vector pDEST22-CaPti1 was co-transformed into yeast strain MaV203 with the prey vector pDEST32-CaERF4 ...
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bioRxiv - Physiology 2021Quote: ... The concentration of PGE2 was normalized by total protein concentration using a BCA assay (#23225, Thermo Scientific, Rockford, IL, USA).
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bioRxiv - Biochemistry 2021Quote: ... supplemented with 0.1% Triton X-100 (Fisher, Waltham, MA, USA) and Halt protease inhibitors (Pierce Thermo Fisher, Rockford, IL, USA) followed by rocking for 30 min at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... DF-1 cells were dissociated and counted using a hemacytometer (1492, Hausser Scientific Partnership, Fisher Scientific, Hanover Park, IL, USA) (Tyler and Hall ...
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bioRxiv - Biophysics 2021Quote: ... was thawed at RT and 100 μl added to 10 ml of Middlebrook 7H9 media (Fisher Scientific, Hanover Park, IL), warmed to 37° C ...
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bioRxiv - Immunology 2020Quote: Cytokines (IL-5 and IL-13) in supernatant of human and murine ILC2 cell cultures were analyzed with ELISA kits from Invitrogen. For human cytokine IL-4 in the supernatant of human ILC2 cell cultures were measured by ELISA kit from BioLegend ...
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bioRxiv - Immunology 2021Quote: ... cells were alternatively pre-activated with IL-2 as above and one of: 1:1 anti-CD3/anti-CD28-coated beads (Gibco Dynabeads Human T-Expander or Human Treg Expander) ...