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Citations for Thermo Fisher :
2051 - 2100 of 10000+ citations for IL 1beta Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... antibodies were buffer exchanged from Tris buffer to PBS buffer using the Zeba spin protocol (ThermoFisher Scientific, Rockford, IL, USA), as amine-containing buffers may interfere with biotinylation ...
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bioRxiv - Immunology 2021Quote: ... TNF-α and IL-6 in culture supernatant was quantified by commercial ELISA kits (Thermo Fisher Scientific, Waltham, MA, USA). The measurement of OD (Optical density ...
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bioRxiv - Immunology 2020Quote: ... submandibular bleeding was performed to collect blood for measurement of serum IL-1β levels by ELISA (Invitrogen 88-7013-22). At 4 hours ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was collected and ELISA was performed to detect IL-6 (8 h post stimulation, Cat # 88-7066-22, Invitrogen) and Lcn2 (24 h post stimulation ...
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bioRxiv - Immunology 2022Quote: One million cells PBMCs were incubated in 24 well plates with 30 IU/mL IL-2 (NCI) and with or without 25 μL of Human T-Activator CD3/CD28 dynabeads (ThermoFisher). Following 48 hours ...
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bioRxiv - Bioengineering 2022Quote: ... resuspended in assay medium (RPMI with 10% FBS and 20 IU/mL IL-2), and centrifuged (300xg, 5 mins) (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Skeletal muscle were stained with Myosin 4 monoclonal antibody (MF20, MyHC-2) (1:200; Thermo Fisher Scientific, Rockford, IL, USA) and Laminin gamma 1 Antibody (A5 ...
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bioRxiv - Plant Biology 2022Quote: ... and dialyzed overnight against water using 3.5 kDa molecular weight cut off Snake Skin dialysis tubing (Thermo Scientific, IL, USA). Dialyzed filtrate was loaded onto a HiPrep SP XL 16/10 cation exchange column (GE Healthcare Piscataway ...
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bioRxiv - Microbiology 2022Quote: ... To measure the protein concentration of IFN-λ1 and -λ2 present in the SN we performed an ELISA following the protocol from the manufacturer: IFN-λ1 (IL-29) from Invitrogen/Thermo Fisher Scientific cat ...
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bioRxiv - Neuroscience 2022Quote: CeA punches were homogenized and assessed for protein concentration using a Pierce Bicinchoninic Acid (BCA) assay (Thermo Scientific, Rockford, IL) per manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: The MMTV sag gene 3’ region was derived from IL-10-/-spleen RNA by conversion to complementary DNA using a SuperScript IV Reverse Transcriptase kit (Invitrogen), and PCR amplification with two separate primer sets ...
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bioRxiv - Neuroscience 2023Quote: ... Lateral striatum was dissected from each hemi-section and placed in HABG media (Hibernate A Media, BrainBits, Springfield, IL, HA500; 2% B27 Supplement, ThermoFisher, Cat #17504044 ...
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bioRxiv - Immunology 2023Quote: ... Primary T lymphocytes stimulated with PHA and IL-2 (2 × 105, 100 µl/well) were resuspended in HBSS buffer (Gibco) containing 0.1% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... Amplification of the cDNA samples was carried out using PowerUpTM SYBRTM Green Mastermix (Thermo Fisher Scientific-A25742, Rockford, IL, USA). The real-time PCR reactions were performed using the QuantStudio™ 3 Real-Time PCR System (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... Astrocytes were washed a further two times in wash buffer before incubation with IL-6-PE (12-7069-82, Invitrogen) and IL-8 FITC (BH0814 ...
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bioRxiv - Immunology 2023Quote: ... penicillin/streptomycin (10 μg/mL) and supplemented with the cytokines IL-4 (500 U/mL) and GMCSF (800 U/mL) (both Gibco) [42] ...
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bioRxiv - Microbiology 2023Quote: ... at the Vienna Research Platform for Metabolomics & Proteomics and analyzed using the Proteome Discoverer v2.2.0.388 (ThermoFisher Scientific, Rockford, IL, USA) at the Life Science Computer Cluster (LiSC ...
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bioRxiv - Cell Biology 2023Quote: ... The LC-MS/MS analysis was performed using an LTQ Orbitrap Elite mass spectrometer (Thermo Scientific, Rockford, IL Waltham, MA) coupled online to an Easy-nLC 1000 in the data-dependent mode ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates were obtained using RIPA buffer with the Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher, Rockford, IL, USA). The conditioned media (1.8 ml ...
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bioRxiv - Immunology 2023Quote: ... and complementary DNA (cDNA) was prepared by using Maxima™ H Minus cDNA Synthesis Master Mix (Thermo Scientific, Rockford, IL). Real-time qPCR (qRT-PCR ...
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bioRxiv - Immunology 2023Quote: 5×105 NIKWT and NIKKO BMDMs were stimulated with 20ng/mL IL-4 for 6 hours before being lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... NIKWT and NIKKO BMDMs were treated with 20ng/mL IL-4 for 5 hours before cells were detached using PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... NIKWT and NIKKO BMDMs were stimulated with IL-4 (20ng/mL) for 24 hours before being lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... NIKWT and NIKKO BMDMs were stimulated with IL-4 (20ng/mL) for 24 hours before lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: 1 × 106 NIKWT and NIKKO BMDMs were stimulated with 20ng/mL IL-4 or PBS for 6 hours before being lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... T cells were expanded in RPMI-1640 media supplemented with 10% heat-inactivated FBS (HI-FBS) as well as recombinant human IL-2 (50 U/mL; ThermoFisher) and IL-15 (1 ng/mL ...
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bioRxiv - Immunology 2022Quote: IFNγ mRNA and protein were measured in IL-2 expanded MAIT cells (with or without stimulation with CD3/CD28 Dynabeads (Gibco)) and their culture supernatant by qPCR or ELISA respectively ...
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bioRxiv - Immunology 2022Quote: ... and activated in complete RPMI supplemented with 30 U/mL IL-2 with anti-human CD3/CD28 dynabeads (11131D, ThermoFisher) at a 1:1 cell-to-bead ratio ...
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bioRxiv - Immunology 2023Quote: ... using permeabilization buffer (eBioscience) and stained with the following cytokine antibodies for 30 min at 4°C: IL-17A (eBio17B7, FITC, Invitrogen), IFN-ψ (XMG1.2 ...
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bioRxiv - Biophysics 2023Quote: ... the total protein concentration was determined using the bicinchoninic acid (BCA) protein assay kit (Pierce/Thermo Fisher Scientific; Rockford, IL). 25µg of protein samples were mixed with 4x LDS sample buffer (Life Technologies ...
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bioRxiv - Evolutionary Biology 2023Quote: ... protein concentration of the CF was determined using the Coomassie (Bradford) Protein assay Kit (Thermo Fisher Scientific, Rockford, IL, USA) and bovine serum albumin (BSA ...
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bioRxiv - Evolutionary Biology 2023Quote: ... protein concentration in the supernatant was determined using the Coomassie (Bradford) Protein assay Kit (Thermo Fisher Scientific, Rockford, IL, USA) and bovine serum albumin (BSA ...
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bioRxiv - Immunology 2023Quote: Cell culture supernatants collected at various time intervals from in vitro stimulated T cells of mice or humans were analyzed for IL-2 by ELISA using kits purchased from ThermoFisher Scientific (mouse IL-2 ELISA kit ...
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bioRxiv - Immunology 2023Quote: ... Cell culture supernatants were collected at the end of culture and analyzed for IL-2 by ELISA using a kit (cat # 88-7025-76) purchased from Thermofisher Scientific.
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bioRxiv - Pharmacology and Toxicology 2024Quote: Cell culture supernatants were collected and analyzed for IL-8 release using a commercially available ELISA kit (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2024Quote: PER2::LUC rhythms were monitored for 42 hours prior to the addition of 10 ng/mL of IL-1α (R&D) or matching vehicle (UltraPure DNase/RNase-Free Distilled Water, Invitrogen). Explants were heat shocked immediately or three hours after IL-1α addition.
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bioRxiv - Bioengineering 2024Quote: ... the amount of IL-10 in the HDL-rich fraction and the (V)LDL-rich fraction was quantified with an IL-10 ELISA (Invitrogen) and used to calculate the fraction of bound IL-10 ...
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bioRxiv - Bioengineering 2024Quote: ... the fluorescence intensity of the stained IFNγ and IL-2 antibodies was quantified using an Attune NxT Flow Cytometer equipped with an autosampler (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... The protein content of the resulting lysate was quantified using the BCA Protein Assay Kit (Thermo Scientific, Rockford, IL, USA) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Complete M-PER buffer was prepared through addition of Protease & Phosphatase Inhibitor Cocktail (1X final) (Thermo Scientific, Rockford, IL, USA) to the M-PER buffer ...
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bioRxiv - Cell Biology 2024Quote: ... 20 ng/mL TPO and 40 ng/mL IL-3 from day 1 to day 6 with a media refresh every 2 days (all cytokines from Thermofisher). On day 8 ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse IgGs were stained with Alexa Fluor 568 conjugated donkey anti-mouse IgG (Invitrogen, Cat#A-10037). Panleucocytes were identified using rat anti-mouse CD45 antibodies (BD Pharmingen Inc ...
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bioRxiv - Neuroscience 2021Quote: ... biotinylated horse anti-mouse IgG (H+L) and biotinylated goat anti-mouse IgG2a (Life Technologies, ThermoFisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... biotinylated horse anti-mouse IgG (H+L) and biotinylated goat anti-mouse IgG2a (Life Technologies, ThermoFisher Scientific).
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bioRxiv - Bioengineering 2022Quote: ... Mouse IgG2a Fc Tag (Acro Biosystems) incubation followed with APC Goat anti-mouse IgG2a Fc Antibody (Invitrogen) staining and RFP expression ...
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bioRxiv - Molecular Biology 2020Quote: ... and mouse antibodies were coupled to Dynabeads coated with Pan Mouse IgG antibodies (Thermo Fisher Scientific, 11042). 50 μL of the appropriate Dynabeads pre-coupled with the indicated antibody were added to the chromatin sample and incubated overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... goat anti-mouse Alexa Fluor 568 or goat anti-mouse Alexa Fluor 488 secondary antibody (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... Mouse monoclonal antibodies were detected with a goat anti-mouse IgG HRP-linked antibody (62-6520; Invitrogen) diluted 1:10,000 (v/v) ...
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bioRxiv - Immunology 2020Quote: ... Isotype control and mouse monoclonal antibody to mouse IFNβ (Clone: MAR1-5A3) were purchased from Thermo scientific. SP600125 and C-16 purchased from Calbiochem ...
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bioRxiv - Microbiology 2022Quote: ... CA) or Alexa Fluor 488 anti-mouse antibody (for LP11 and mouse anti-myc; Invitrogen, Waltham, MA) at a 1:250 dilution in FACS medium ...