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Citations for Thermo Fisher :
2201 - 2250 of 3282 citations for HSD17B4 siRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: CSE knockdown was performed using human siRNA targeting CTH (Ambion-Life Technologies, ID: s3710 and s3712). The control siRNA (siCtrl ...
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bioRxiv - Cell Biology 2024Quote: ... siRNA (72 nM) was transfected using Lipofectamine 3000 reagent as per the manufacturer’s protocol (Invitrogen #L3000001). The pcDNA5 FRT/TO MycLAP-STIL plasmids was ordered from Addgene (#80266) ...
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bioRxiv - Physiology 2024Quote: ... or an equivalent concentration of a scrambled control siRNA (#4390844, Silencer Select Negative Control #1, Ambion) using Lipofectamine RNAimax (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... si-rab27 and sc-rab27 were synthesized using the Silencer siRNA construction kit (Thermo Fisher Scientific) using the primers in Table S1 ...
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bioRxiv - Molecular Biology 2023Quote: Silencing RNA for NCL (human NCL silencer select) and siRNA controls were obtained from Thermo Fisher. For siRNA-mediated silencing ...
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bioRxiv - Molecular Biology 2023Quote: ... siRNAs were reverse transfected at a final concentration of 5nM using Lipofectamine RNAiMAX reagent (Life Technologies). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... The following probesets were used: PPIB (siRNA target, SA-100 03, Invitrogen™, Thermo Fisher Scientific), HPRT ...
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bioRxiv - Cell Biology 2023Quote: ... Vero cells were transfected in 24-well plates with 10 pmol siRNA using Lipofectamine 3000 (Invitrogen). After 2 hours ...
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bioRxiv - Cell Biology 2023Quote: All siRNA-based depletions were performed at 20 nM final concentration using Lipofectamine RNAiMAX (Life Technologies) transfection reagent according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Knockdown experiments were transfected with 8 pmol siRNA (Scramble: AM4635, PLD1: 4390824, ThermoFisher, Waltham, MA, USA) and 0.4 µL Lipofectamine 2000 per well for 72 hours prior to imaging.
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bioRxiv - Cell Biology 2023Quote: All cells were transfected with small interfering RNAs (siRNAs) using Lipofectamine™ RNAiMAX Transfection Reagent (Invitrogen) according to the manufacturer’s instructions for 3 days ...
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bioRxiv - Cancer Biology 2023Quote: Cells were seeded in 96-well plate and transfected with siRNA using lipofectamine RNAi MAX (Invitrogen) as described in the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... hVSMCs were transfected with 60nM of siRNA or miRNA mimic/inhibitor using RNAiMAX transfection reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... plasmids and siRNA were transfected in mammalian cells at 70% confluency using Lipofectamine 3000 (Invitrogen, #L3000001). PLK4 siRNA and CEP152 siRNA oligonucleotides (Merck ...
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bioRxiv - Cancer Biology 2023Quote: ... and ABL2 ON-TARGETplus siRNA (Horizon Discovery) were introduced using Lipofectamine™ RNAiMAX reagent (ThermoFisher Scientific). Pooled knockout lines were generated by infection with lentiviruses expressing CAS9 and appropriate sgRNAs ...
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bioRxiv - Cell Biology 2023Quote: ... and E2F1 mRNA were depleted using pre-designed Silencer® Select siRNAs (Ambion, Life Technologies, USA). The negative control siRNA (siCTL ...
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bioRxiv - Cell Biology 2023Quote: ... and E2F1 mRNA were depleted using pre-designed Silencer® Select siRNAs (Ambion, Life Technologies, USA). The negative control siRNA (siCTL ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were seeded and transfected with 2.5 nM siRNA the next day using Lipofectamine2000 (Thermo Fisher) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... NCL siRNA or a scrambled control at 25 nM were transfected using DharmaFECT 4 (Thermo Fisher). 24h post transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... The following probesets were used: PPIB (siRNA target, SA-100 03, Invitrogen™, Thermo Fisher Scientific), HPRT ...
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bioRxiv - Immunology 2023Quote: ... siRNA-treated target NK or T cells were labelled with 2 uM CellTrace Violet (Thermo Fisher), treated with 1 uM Concanamycin A (Santa Cruz ...
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bioRxiv - Cell Biology 2024Quote: ... siRNA-mediated ATG7-silencing was performed in HUVECs with 5mM of siATG7 or scrambled control (Ambion) and the Dharmafect-4 transfection reagent (Dharmacon ...
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bioRxiv - Molecular Biology 2024Quote: ... Silencer Select small interfering RNA (siRNAs) for LOX-1 and AT1a (Thermo Fisher Scientific, MA, USA) were transfected into cells in medium without serum or antibiotics using Lipofectamine RNAiMAX (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: Human primary Müller cells (P3) after siRNA treatments were incubated with JC-1 dye (#T3168, Invitrogen) at a concentration of 1.0 μg/mL in DMEM at 37 °C for 30 minutes to measure the mitochondrial membrane potential ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were infected with packaged lentivirus or transfected with siRNA using Lipofectamine 3000 (Invitrogen, USA) when they were cultured to 60%-70% confluence.
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bioRxiv - Biochemistry 2024Quote: Depletion of endogenous proteins was achieved through transfection of small interfering RNA (siRNA) with RNAiMAX (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... or a pooled combination of three Cflar-targeting siRNAS (Thermo Fisher #’s 66164, 66073, and 65977) targeting exons 2 ...
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bioRxiv - Microbiology 2024Quote: ... All miRNAs and siRNAs were transfected using the Lipofectamine RNAi-MAX (Invitrogen; Ref. No. 13778-075) as described previously (27) ...
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bioRxiv - Microbiology 2024Quote: ... The cells were then transfected with 50 nM control and ATF4 SilencerSelect siRNA (ThermoFisher Scientific, #s1702) using Lipofectamine RNAi Max transfection reagent (Invitrogen ...
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bioRxiv - Plant Biology 2019Quote: ... siRNAs were then specifically purified by using the mirVana™ miRNA Isolation Kit (Life Technologies, Carlsbad, CA). Each step of this process was followed by gel electrophoresis (TAE 1X ...
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bioRxiv - Cell Biology 2020Quote: ... 6 or 9 µl of 20 µM siRNA and 4 µl of Lipofectamine RNAi MAX (13778150, Invitrogen) were suspended in 100 µl of water (W4502 ...
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bioRxiv - Cell Biology 2020Quote: ... The expression of proteins of interest was suppressed using 100 nM siRNA and lipofectamine 3000 (ThermoFisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were transfected with siRNA duplexes targeting the corresponding protein or scrambled mixes using Lipofectamine RNAiMax (Invitrogen) reagents as per manufactures recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... The Silencer Select negative control siRNA number 1 was used as a negative control (Ambion, ThermoFisher Scientific).
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bioRxiv - Cell Biology 2020Quote: ... The siRNAs targeting porcine USP9X (ENSSSCG00000012251) transcript were designed using BLOCK-iT™ RNAi Designer (ThermoFisher Scientific) and synthesized from Eurofins Genomics ...
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bioRxiv - Cell Biology 2020Quote: ... siRNAs were diluted to a final concentration of 100nM in Opti-MEM Reduced Serum Medium (ThermoFisher, 31985070) before mixing with an equal volume of Optimem:RNAiMax (ThermoFisher ...
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bioRxiv - Microbiology 2020Quote: ... 5 pmol of total siRNA were transfected into macrophages using Lipofectamine RNAiMAX transfection reagent (Thermo Fisher Scientific) following the manufacturer’s protocol ...
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TSG101 Associates with PARP1 and is Essential for PARylation and DNA Damage-induced NF-κB ActivationbioRxiv - Molecular Biology 2021Quote: ... Each screening plate contained several controls such as a non-targeting siRNA (Silencer Negative Control from Ambion), a cell-death inducing siRNA mixture ...
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bioRxiv - Cell Biology 2022Quote: ... All the siRNA silencing and plasmid transfection studies was done with lipofectamine 2000 purchased from Thermo Fisher Scientific.
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bioRxiv - Molecular Biology 2020Quote: siRNA purchased from Eurofins MWG Operon (Supplementary Table 2) were transfected into cells using Lipofectamine RNAiMAX (Invitrogen) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... 79] were thawed and transfected with siRNAs at 5nM final concentration using Lipofectamine RNAiMAX transfection reagent (Invitrogen). Approximately 20,000 cells were plated in each well of a 384-well plate (Greiner Bio-One ...
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bioRxiv - Microbiology 2020Quote: ... the Vero E6 cells were transfected with 100 nM siRNA using Lipofectamine RNAiMAX transfection reagent (Invitrogen, USA), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Transfection of the pooled siRNAs into macrophages was performed using Lipofectamine RNAiMAX transfection reagent (Thermo Fisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... then treated with siRNA or scramble control to final concentration of 20nM with RNAiMax (Invitrogen, Carlsbad, CA) for 12 hours ...
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bioRxiv - Molecular Biology 2019Quote: ... HeLa cells were transfected with a mix containing siRNA (20 nM) and RNAi Max reagent (Life Technologies) according to the reverse transfection protocol then collected 72 h after for analysis ...
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bioRxiv - Biophysics 2019Quote: ... 106 cells plated per well of a 6-well plate were transfected with 150 pmol siRNAs (Ambion: Negative Control #AM4611 ...
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bioRxiv - Cell Biology 2019Quote: ... 3 different siRNAs Silencer per gene were transfected in cells using Lipofectamine 2000 (ThermoFisher Scientifics, 11668-019) at the final concentration of 10 nM ...
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bioRxiv - Cell Biology 2020Quote: ... cells were treated with siRNA (control siFF and siUHRF1) and RNAi Max according to manufacturer’s protocol (Invitrogen). After 48 hours of siRNA treatments ...
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bioRxiv - Cell Biology 2019Quote: ... 40 pmoles of siRNAs were transfected into cells with Lipofectamine RNAiMAX (Thermo Fisher Scientific, cat# 13778-150) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: Transfection of siRNAs was performed in six-well tissue culture plates using Lipofectamine RNAiMAX transfection reagent (Invitrogen). The final concentration of siRNA was 10 nM ...