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Citations for Thermo Fisher :
2201 - 2250 of 4555 citations for Dnp pro leu gly cys me his ala D arg nh2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... were separated using NuPAGE Bis-Tris gels (4 – 12 %) with 3-(N-morpholino)propanesulfonic acid (MOPS) or 2-(N-morpholino)ethanesulfonic acid (MES) running buffer (Thermo Fisher Scientific) followed by transferring to polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2022Quote: ... Pellets expressing SKA1/2 and GFP-His-SKA2 proteins were combined and the cells were lysed by sonication on ice in lysis buffer (20 mM Tris pH 8.0, 500 mM NaCl, 2 mM 𝛽-ME, supplemented with 1X protease Inhibitor (Thermo Scientific, #AA32965). Lysate was then clarified by centrifuge at 58387 × g ...
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bioRxiv - Cell Biology 2022Quote: ... cell lysates were subjected to SDS-PAGE electrophoresis using NuPAGE 4-12% Bis-Tris gels and MES running buffer (Thermo Fisher Scientific) according the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... All contrast agents were activated for bioconjugation with purified L-PGDS using by addition of 100mM EDC and 250mM sulfo-NHS dissolved in amine- and carboxyl-free MES buffer (Thermo Scientific™) with pH adjusted to 6.0 to a final ratio of 1:5 [50] ...
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bioRxiv - Microbiology 2022Quote: ... ME) using the EasySep™ mouse T cell isolation kit (StemCell, Cambridge, MA) and labeled with carboxyfluoroscein succinimidyl ester (CFSE; Life Technologies) as described previously [18] ...
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bioRxiv - Microbiology 2022Quote: ... 4-12% or 12% NuPAGE® Bis-Tris mini gel in a XCell SureLock® Mini-Cell filled with either 1x MES or 1x MOPs SDS running buffer (Invitrogen, USA) was used and run at 140 V for 90 min ...
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bioRxiv - Molecular Biology 2022Quote: ... was placed in the electrophoretic tank and the tank was filled with 1x NuPAGE® MES Running Buffer (ThermoFisher Scientific, Paisley, UK) (diluted from the 20x stock in ultrapure water) ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then loaded into NuPAGE Novex 4-12 % Bis-Tris Protein Gels in MOPs or MES SDS running buffer (both Thermo Fisher Scientific) and SDS-PAGE was conducted by the application of 200 V for 50 minutes or 120 V for 1.5 hours ...
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bioRxiv - Neuroscience 2023Quote: ... SDS-PAGE was conducted by running samples on NuPAGE Novex 4-12 % Bis-Tris Protein Gels in MOPs or MES SDS running buffer (both Thermo Fisher Scientific) at 120 V for 1.5 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples of extracts were mixed with sample buffer and DTT and denatured at 70 °C for 10 min before being subjected to SDS-PAGE (60 µg protein/sample lane on NuPAGE 12% Bis-Tris Gel) for ∼4 minutes at 200V 218 mA (∼44 W) in NuPAGE™ MES SDS Running Buffer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were run on NuPage™ Novex™ 4-12% Bis-Tris protein gels in MES buffer or on E-PAGE 8% 48-well gels (ThermoFisher). For native running conditions ...
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bioRxiv - Molecular Biology 2023Quote: ... was added to the ribosome samples followed by separation on 8% (w/v) Bolt Bis-Tris gel in MES buffer (Thermo Fisher Scientific), followed by transfer onto a 0.45 μm PVDF Immobilon-P membrane (Bio-Chapter 691Rad) ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins present in supernatant were solubilized with Laemmli sample buffer and 40 µl of each sample were resolved by NuPAGE 4-12% Bis-Tris gel (MES buffer, Thermo Scientific, WG1401) and transferred onto nitrocellulose membranes ...
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bioRxiv - Cell Biology 2024Quote: ... SDS-PAGE was performed using NuPAGE 4–12% Bis-Tris gradient gel and a compatible MES SDS buffer system (both from Thermo Fisher Scientific). Resolved proteins were electrotransferred to a nitrocellulose membrane ...
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bioRxiv - Molecular Biology 2024Quote: ... The loaded hAgo2 RNPs were captured using a biotinylated antisense oligonucleotide (2’-O-Me RNA, IDT) immobilized on high capacity neutravidin agarose (Thermo Fisher Scientific), washed with 3 different buffers (first ...
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bioRxiv - Molecular Biology 2024Quote: SDS–PAGE electrophoresis was performed with TSPs (10 μg) and purified proteins (1.5 μg) using NuPAGETM 1X MES SDS Running Buffer (Invitrogen, Carlsbad, CA, USA) and NuPAGETM 4–12% Bis/Tris gels (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... 4-12% Bis-Tris gels (NuPage) were run at 120-150V in MOPS or MES SDS running buffer (Invitrogen #NP0002 and #NP000202). Gels were transferred to nitrocellulose membranes (BioRad #1704158 ...
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bioRxiv - Cell Biology 2024Quote: Protein samples were separated by SDS-PAGE using NuPAGE 4–12% Bis-Tris gels and NuPAGE MES SDS Running Buffer (Thermo Fisher Scientific). Typically ...
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bioRxiv - Developmental Biology 2021Quote: ... The bands were excised from the gel and subjected to a mass spectrometric analysis with Orbitrap Velos Pro (Thermo Fisher Scientific). For protein identification ...
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bioRxiv - Molecular Biology 2020Quote: ... was carried out on an EASY-nLC1000 chromatograph connected to a Velos Pro-Orbitrap Elite hybrid mass spectrometer with nano electrospray ion source (all instruments from ThermoFisher Scientific). The LC-MS/MS samples were separated using a two-column setup ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then rinsed 4 x 15 min in PBS using the nuclear counterstain TO-PRO-3 Iodide (1:2000, Thermo Fisher). For preservation of immunofluorescence ...
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bioRxiv - Biochemistry 2021Quote: ... Resulting peptides were analyzed by online nanoliquid chromatography coupled to tandem MS (UltiMate 3000 and LTQ-Orbitrap Velos Pro, Thermo Scientific). Peptides were sampled on a 300 μm × 5 mm PepMap C18 precolumn and separated on a 75 μm × 250 mm C18 column (PepMap ...
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bioRxiv - Biochemistry 2020Quote: ... Reduced N-glycans were profiled in technical triplicates using porous graphitised carbon liquid chromatography-tandem mass spectrometry (LC-MS/MS) in negative ion polarity on an LTQ Velos Pro ion trap mass spectrometer (Thermo Scientific) (Hinneburg et al. ...
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bioRxiv - Neuroscience 2021Quote: ... and placed in the QuantStudio 6 Pro Real-Time PCR system (all reagents for RT-qPCR were purchased from ThermoFisher Scientific). The PCR cycle protocol was ...
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bioRxiv - Cancer Biology 2020Quote: ... Supernatants were collected and cytokines were measured by either Bio-Plex Pro (BIO RAD, #17AL001M) or ProcartaPlex (Thermo Fisher, PPX-24).
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bioRxiv - Plant Biology 2021Quote: Accumulation of ROS was measured with a luminol based reporter system and a microplate reader (Tecan Infinite F200 PRO or Luminoscan Ascent 2.1, Thermo Fisher Scientific) as describe previously (Ranf et al. ...
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bioRxiv - Microbiology 2020Quote: ... Resulting peptides were analyzed by online nanoliquid chromatography coupled to tandem MS (UltiMate 3000 and LTQ-Orbitrap Velos Pro, Thermo Scientific). Peptides were sampled on a 300 μm x 5 mm PepMap C18 precolumn and separated on a 75 μm x 250 mm C18 column (PepMap ...
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bioRxiv - Cancer Biology 2022Quote: ... was loaded onto EvoTip trapping columns before separation with the EvoSep One nanoLC system (EvoSep, Odense, Denmark) coupled to an Orbitrap Fusion Lumos mass spectrometer with a FAIMS-PRO interface (Thermo Fisher). Peptides were eluted over a 44-min gradient ...
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bioRxiv - Biochemistry 2022Quote: Samples were analyzed on a LC-MS/MS system consisting of an Orbitrap Velos Pro Mass Spectrometer (Thermo Scientific, Waltham, MA) and a Dionex Ultimate3000 nano-LC system (Thermo Scientific ...
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bioRxiv - Neuroscience 2022Quote: LC-MS/MS analyses were conducted using either a QExactive Plus Orbitrap (QE, RNase-digested polysomes) or a Velos Pro Elite Orbitrap (Elite, virus polysomes and HEK293 aggregates) mass spectrometer (Thermo Fisher) coupled online to a nanoAcquity UPLC system (Waters Corporation ...
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bioRxiv - Genomics 2022Quote: Hair cell staining and quantification were performed as described (Pei et al., 2016) using YO-PRO-1 (Life Technologies. Cat#: Y3603). For hair cell regeneration analysis ...
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bioRxiv - Cancer Biology 2020Quote: ... nuclear counterstaining was performed by incubation in TO-PRO™-3 Iodide (642/661; cat. #T3605; Thermo Fisher Scientific, Waltham, MA) diluted in DMSO (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... iNeuron viability was assessed by adding 10 ug/mL Hoechst and 1 μM TO-PRO-3 (ThermoFisher Scientific cat. no. T3605) in DPBS ...
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bioRxiv - Neuroscience 2022Quote: Cell viability in HeLa cells treated with pro-death stressors was measured by staining cells with annexin V AlexaFluor-488 conjugate (Thermo Fisher) as described previously 39 ...
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bioRxiv - Cell Biology 2022Quote: ... The P1-P4 clones were mixed with the corresponding P4r-P2 and the dexamethasone-inducible destination vector pBAV154 (Vinatzer et al., 2006) in a three-way Gateway LR reaction (MultiSite Gateway® Pro from Invitrogen by Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... and cloned into the pBSDONR P1-P4 (Gu and Innes, 2011) by Gateway BP reaction (MultiSite Gateway® Pro from Invitrogen by Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Then 10 µL of each sample was added to 490 µL of PBS containing 0.1 nM TO-PRO®-3 (Life Technologies) and 0.5 µM DiBAC4(3 ...
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bioRxiv - Developmental Biology 2020Quote: ... An orthogonal 2D-LC-MS/MS analysis was performed with the Dionex Ultimate 3000 UHPLC system coupled with the ultra-high-resolution nano ESI LTQ-Velos Pro Orbitrap Elite mass spectrometer (Thermo Scientific).
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bioRxiv - Immunology 2021Quote: ... and 4 & 105 cells pro well were plated in tissue culture-treated six-well plates (Nunc Multidishes with UpCell Surface, Thermo Fisher) in 4 ml of complete RPMI-1640 medium (10% fetal calf serum (FCS) ...
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bioRxiv - Biochemistry 2021Quote: LC MS/MS was carried out using the Fusion Lumos Orbitrap mass spectrometers with the FAIMS Pro interface (Thermo Fisher, USA) and as described previously [26] ...
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bioRxiv - Neuroscience 2021Quote: ... and immersed in reagent-2 (diluted 1:2 in PBS) for 6-24 h before incubated in reagent-2 containing TO-PRO-3 (1:5,000, Thermo Fisher Scientific) for additional 7-10 days ...
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bioRxiv - Plant Biology 2021Quote: ... Cleared tissue was washed in PBS and then put into a PBS solution containing 0.1% SR2200 and a 1/1000 dilution of the nuclear stain TO-PRO-3 iodide (Thermo Fisher Scientific) for 20 minutes ...
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bioRxiv - Microbiology 2022Quote: ... Protein abundance samples were fractionated with Field Assymetric Ion Mobility Spectrometry (FAIMS) fractionation with a FAIMS Pro device (Thermo Fisher Scientific). Each pooled sample was analyzed four times with four FAIMS compensation voltages (CV ...
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bioRxiv - Biochemistry 2022Quote: ... in an Orbitrap Fusion Lumos equipped with a high-Field Asymmetric Ion Mobility Spectrometry (FAIMS Pro) interface69,70 and coupled to a Proxeon NanoLC 1200 (Thermo Fisher Scientific). A 100 μm capillary column was packed with 35 cm of Accucore 150 resin (2.6 μm ...
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bioRxiv - Biochemistry 2022Quote: ... coupled by a Nanospray Flex ion source to an FAIMS pro interface equipped Obritrap Exploris 480 mass spectrometer (Thermo Fisher Scientific). Operated in data-dependent mode ...
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bioRxiv - Biochemistry 2022Quote: All samples were analyzed using a Vanquish Neo UHPLC operated in direct injection mode and coupled to the Orbitrap Exploris 480 mass spectrometer equipped with a FAIMS Pro interface (ThermoFisher Scientific). Analyte separation was performed using either the new generation prototype 110cm ...
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bioRxiv - Microbiology 2022Quote: ... The virus-containing samples (50 μl) were serially diluted in 450 μl aliquots of serum-free Opti-Pro medium (Gibco, UK), after which 200 μl from each dilution were added in duplicates to the cells ...
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bioRxiv - Microbiology 2022Quote: ... Viral RNA from each time point was purified by using PureLink™ Pro 96 Viral RNA/DNA Purification Kit (Thermo Fisher) and then the viral copy number in each sample was estimated by qRT-PCR using TaqPath™ 1-Step RT-qPCR Master Mix (Thermo Fisher ...
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bioRxiv - Pathology 2022Quote: ... qRT–PCR was performed using qPCRBIO SyGreen Mix (Protech) and the QuantStudio™ 6 Pro Real-Time PCR System (Applied Biosystems/Thermo Fisher Scientific Inc. ...
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bioRxiv - Synthetic Biology 2023Quote: ... The bioluminescent signal was detected with a Tecan Infinite M1000 Pro reader in 384-well Armadillo Plates (BC-3384, Thermo Scientific).