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Citations for Thermo Fisher :
2001 - 2050 of 4555 citations for Dnp pro leu gly cys me his ala D arg nh2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... AMR Inc.) and applied directly into a hybrid linear ion trap mass spectrometer (LTQ Orbitrap Velos Pro; Thermo Fisher Scientific) with Advanced Captive Spray SOURCE (AMR Inc. ...
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bioRxiv - Microbiology 2020Quote: ... was on-line coupled to an Orbitrap Velos Pro (NTM samples) or Orbitrap Fusion Lumos instrument (Mtb sample) (Thermo Scientific). MS data were acquired in a data-dependent strategy selecting the fragmentation events based on the precursor abundance in the survey scan (275–1850 Th for NTM samples and 375–1700 Th for the Mtb sample) ...
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bioRxiv - Cancer Biology 2020Quote: ... The Ba/F3 pro-B cell line was obtained from Leibniz-Institute DSMZ and grown in RPMI-1640 (Life Technologies) with 10% FBS (Life technologies) ...
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bioRxiv - Biochemistry 2021Quote: ... IL3-dependent murine pro-B cell (Ba/F3 cells, ATCC) were maintained in Roswell Park Memorial Institute (RPMI) media (Thermofisher) supplemented with 10% FBS and IL-3 and 1% penicillin/streptomycin ...
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bioRxiv - Molecular Biology 2020Quote: ... Peptides were eluted from the column and electrosprayed into a Velos Pro Linear Ion Trap Mass Spectrometer (Thermo Fisher Scientific). Data was acquired using data-dependent acquisition (DDA ...
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bioRxiv - Microbiology 2021Quote: ... Samples were run at 120V for 1.5h and were stained using the Pro-Q Emerald 300 Lipopolysaccharide Gel Stain Kit (Molecular Probes) following manufacturer directions ...
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bioRxiv - Immunology 2021Quote: ... tissue was homogenized in 1000 μL of PBS with 4 homogenizing beads (Pro200H Series homogenizer; Pro Scientific) and the implants underwent sonication in 500 μL 0.3% Tween-80 (ThermoFisher Scientific) in TSB ...
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bioRxiv - Immunology 2020Quote: ... DNA was stained for 5 minutes in PBS with 1 ug/ml of DAPI and coverslips mounted with Pro-Long Gold (Invitrogen). Imaging was performed using a Leica SPE2 confocal microscope using LAS X version 3.3.0 software (Leica Microsystems ...
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bioRxiv - Immunology 2020Quote: ... or homogenized with a Bio-Gen PRO200 homogenizer with an attached 5mm X 75mm flat bottom generator probe (Pro-Scientific) and then loaded into 4-12% Bolt Bis-Tris gels (Invitrogen) for gel electrophoresis ...
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bioRxiv - Microbiology 2021Quote: ... and glycol-proteins and the DNase/Proteinase K treated matrix samples with the Pro-Q Emerald 300 glycoprotein stain (ThermoFisher).
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bioRxiv - Systems Biology 2022Quote: ... Mass spectrometry was performed with a high-field asymmetric waveform ion mobility spectrometry (FAIMS) Pro-equipped Orbitrap Eclipse (ThermoFisher Scientific) in positive ion mode using data-dependent acquisition with 2 second top speed cycles ...
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bioRxiv - Biochemistry 2022Quote: ... supernatants and cell extracts were analysed with an Agilent 1100 HPLC connected to an Orbitrap LTQ Velos Pro (Thermo Fisher). The column a Hilicon iHILIC prototype (100 × 2.1 mm ...
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bioRxiv - Immunology 2022Quote: ... gels were rehydrated in 3% acetic acid and successively stained using Pro-Q Emerald 300 Lipopolysaccharide Gel Stain Kit (Invitrogen) and Coomassie Brilliant Blue R-250 ...
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bioRxiv - Physiology 2022Quote: ... The gel was first stained for phosphorylated proteins using Pro-Q™ Diamond Phosphoprotein Gel Stain (Molecular Probes® P33301) with PeppermintStick™ Phosphoprotein Molecular Weight Standards (Molecular Probes® ...
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bioRxiv - Physiology 2024Quote: ... TaqMan-based real time quantitative polymerase chain reactions (qRT-PCR) were then performed using a QuantStudio 7 Pro Real-Time PCR System (ThermoFisher). The housekeeping gene Vinculin was used as an internal control for cDNA quantification and normalization of gene amplified products.
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bioRxiv - Molecular Biology 2024Quote: ... the complexes were TCA precipitated and associated proteins were identified by LC-MS/MS at the Taplin Mass Spectrometry Facility (Harvard Medical School) using an LTQ Orbitrap Velos Pro ion-trap mass spectrometer (ThermoFisher) and Sequest software46 ...
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bioRxiv - Microbiology 2024Quote: ... We run SDS-PAGE on each gel and stain the separated LPS bands using Pro-Q Emerald 300 Lipopolysaccharide Gel Stain Kit (Thermofisher) according to the manufacturer’s instructions ...
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Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: ... amino acids 23-429) or pro- BMP10 (NP_055297.1, amino acids 22-424) was cloned into pcDNA3.1+ (Invitrogen/Thermo Fisher Scientific) downstream of the rat serum albumin signal peptide ...
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bioRxiv - Cell Biology 2024Quote: ... siRNA-treated cells were washed twice with 37°C M1 and replaced with M1 containing 1 μM TO-PRO-3 iodide (ThermoFisher) or washed once with 37°C M2 containing 5 mM ethylene glycol-bis(2-aminoethylether)-N ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 µL of 1 mM TO-PRO-3 Iodide in DMSO (Thermo) was added to stain dead cells and 1uL of DRAQ5 (Invitrogen) was added to stain viable cells ...
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bioRxiv - Biochemistry 2023Quote: To study the effect of PfRKIP on the transphosphorylation activity of PfCDPK1 we used Pro-QTM diamond stain (ThermoFisher Scientific). PfCDPK1 was incubated with different concentrations PfRKIP (2 μg-16 μg ...
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bioRxiv - Cell Biology 2023Quote: ... according to the published protocol 38optimized for higher proteome depth and 16-plex multiplicity using Tandem Mass Tag (TMT) pro (ThermoFisher) and using a gradient for thermal denaturation as described previously61,62.
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bioRxiv - Developmental Biology 2023Quote: ... Dissociated cells were quantified in a Burker chamber and then plated in 24-multiwell plates (Falcon) at 100 - 300 cells/uL, in pro-proliferative medium (1:1 DMEM-F12, 1X Glutamax (Gibco), 1X N2 supplement (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... using a nanoACQUITY (Waters Corporation, Milford, MA) online coupled with an Orbitrap Velos Pro hybrid ion trap mass spectrometer (ThermoFisher). The precursor ions were selected automatically by the instrument ...
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bioRxiv - Biophysics 2023Quote: ... the tissue was incubated for 5 min with 1 µM TO-PRO-3 iodide (Invitrogen, Life Technologies Corporation, Oregon, USA) followed by washing with 1x DPBS (nuclei data not shown) ...
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bioRxiv - Neuroscience 2023Quote: ... Mass spectrometry was performed with a high-field asymmetric waveform ion mobility spectrometry (FAIMS) Pro-equipped Eclipse Orbitrap mass spectrometer (ThermoFisher) in positive ion mode using data-dependent acquisition (DDA ...
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bioRxiv - Microbiology 2023Quote: ... We visualized samples using 15 μL of LPS preparation on a 4%-20% Tris-glycine mini gel using the Pro-Q Emerald 300 Lipopolysaccharide Gel Stain Kit (ThermoFisher) (73).
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bioRxiv - Neuroscience 2023Quote: ... Peptides were eluted from the tip of the column and nano electrosprayed directly into an Elite or Velos Pro mass spectrometer (ThermoFisher) by application of 2.5kV voltage at the back of the column ...
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bioRxiv - Molecular Biology 2024Quote: ... RT-PCR was performed for both microRNA and genes using the QuantStudio 7 Pro Real-Time PCR System (Applied Biosystems) and TaqMan Gene Expression Assays (Applied Biosystems ...
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bioRxiv - Microbiology 2024Quote: ... The marker proteins were determined by an LTQ Velos Pro mass spectrometer equipped with a Nano ESI source (Thermo Scientific). The samples were loaded onto a trap column (Acclaim PepMap RSLC 75 μm × 50 cm ...
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bioRxiv - Microbiology 2024Quote: ... RNA was extracted using Invitrogen PureLink Pro 96 Viral RNA/DNA Purification Kits on an EpiMotion or a MagMAX viral/pathogen nucleic acid purification kit (ThermoFisher) on a Kingfisher Machine ...
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bioRxiv - Microbiology 2024Quote: ... The samples were separated on 12% or 20% (w/v) SDS-PAGE and stained using a Pro-Q Emerald 300 Lipopolysaccharide Gel Stain Kit (Invitrogen) for imaging.
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bioRxiv - Microbiology 2024Quote: ... pellets re-suspended in minimal volume and mounted onto glass slides with Pro Long Gold Antifade (Invitrogen, Cat No: P36930) with coverslips ...
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bioRxiv - Immunology 2024Quote: ... Reactions were run in triplicates and analyzed on a QuantStudio™ 6 Pro sequence detection system (Applied Biosystems, Waltham, MA) equipped with a 384-well block ...
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bioRxiv - Plant Biology 2024Quote: ... coupled to an Orbitrap Exploris 480 mass spectrometer with a FAIMS (Field asymmetric ion mobility separation) Pro interface (Thermo Fisher). Peptides were pre-concentrated on an Acclaim PepMap 100 pre-column (Thermo Fisher ...
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bioRxiv - Genetics 2024Quote: ... ELISA for pro-inflammatory cytokines IL-6 and IL-8 were performed in technical triplicate according to manufacturer’s protocol (Thermo Fisher KHC0061 and Thermo Fisher KHC0081 ...
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bioRxiv - Cell Biology 2024Quote: ... No mycoplasma were detected in cultures by 4′,6-diamidino-2-phenylindole (DAPI) or TO-PRO-3 (Thermo Fisher Scientific) staining ...
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bioRxiv - Immunology 2024Quote: ... gels were digested and assessment of cell viability was performed by staining with 20 nM TO-PRO-3 (Thermo Scientific). The number of alive cells recovered from culture was quantified by flow cytometry ...
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bioRxiv - Bioengineering 2024Quote: ... were used to detect mRNA transcript levels on a QuantStudio™ 6 Pro Real-Time PCR System (Applied Biosystems; A43180). Results were analyzed using the ΔΔCt methodology with GAPDH used as a housekeeping gene ...
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bioRxiv - Biochemistry 2024Quote: ... TaqMan-based real time quantitative polymerase chain reactions (qRT-PCR) were then performed using a QuantStudio 7 Pro Real-Time PCR System (ThermoFisher). The housekeeping gene Vinculin was used as an internal control for cDNA quantification and normalization of gene amplified products.
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bioRxiv - Cancer Biology 2024Quote: ... qPCR reactions were performed using QuantStudio 7 Pro applied biosystems and Fast SYBR-Green Mastermix (cat # 4385610, Thermo Fisher Scientific). For qPCR analysis ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative polymerase chain reaction (qPCR) was performed using QuantStudio Pro 6 Real-Time PCR System (Thermo-Fisher Scientific, Waltham, MA). Relative quantification of target genes was calculated by the ΔΔCt method with Hprt and/or 18S rRNA as internal controls ...
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bioRxiv - Cell Biology 2020Quote: Total cellular mRNA was isolated from cells grown in 2-D and 3-D culture conditions using the GeneJET RNA purification kit (Thermo Fisher Scientific). For analysis of germ layer markers ...
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bioRxiv - Synthetic Biology 2020Quote: ... cells from 2-D or 3-D cultures were harvested from one well of a 6-well plate and dissociated with Accutase (Thermo Fisher #A1110501), washed three times with 1x PBS ...
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bioRxiv - Immunology 2020Quote: ... D-PBS (D-PBS without Ca++ and Mg++) supplemented with 2% FBS and 3 mM cell culture grade EDTA (Life Technologies; Thermofisher) prior to monocyte isolation ...
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bioRxiv - Plant Biology 2023Quote: HEK293T cells were seeded into 96 well plates and incubated in D-MEM/Ham’s F-12 in D-MEM/Hams F-12 (FisherScientific, Pittsburgh, USA) supplemented with 10% FBS (Fisher Scientific,Pittsburgh, USA) until reaching about 80% confluency ...
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bioRxiv - Neuroscience 2024Quote: ... Cryo-EM data of D-TLKIVWR and D-TLKIVWS fibrils were collected on a Titan Krios transmission electron microscope (Thermo Fisher Scientific) located at the National Center for Cryo-EM Access and Training ...
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bioRxiv - Developmental Biology 2021Quote: ... The ORF of red fluorescent protein gene (RFP)-fused Antp was inserted into a pIZ/V5-His vector (Invitrogen) driven by the OpIE2 promoter ...
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bioRxiv - Molecular Biology 2021Quote: ... and the C-terminal foldon trimerization motif followed by an 8×His-tag was cloned into the pcDNA3.1(+) expression vector (Invitrogen). Furthermore ...
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bioRxiv - Biochemistry 2020Quote: ... His-tagged Gβγ was expressed in Sf9 cells for 2 days following baculovirus infection (pFastBacDual, Bac-to-Bac, Invitrogen). Cells were harvested by centrifugation and lysed by sonication in 20 mM Tris-HCl pH 8.0 ...