Labshake search
Citations for Thermo Fisher :
2451 - 2500 of 4555 citations for Dnp pro leu gly cys me his ala D arg nh2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... masses were verified via matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (Applied Biosystems - Voyager-DE™ PRO) (Figure S1 ...
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bioRxiv - Cancer Biology 2022Quote: Dried peptides were resuspended in 0.1% aqueous formic acid and subjected to LC– MS/MS analysis using an Orbitrap Eclipse Tribrid Mass Spectrometer fitted with Ultimate 3000 nano system and a FAIMS Pro interface (all Thermo Fisher Scientific) and a custom-made column heater set to 60°C ...
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bioRxiv - Plant Biology 2022Quote: ... The entry vectors containing the sgRNAs were then cloned into the destination vector by the LR recombination reaction using the MultiSite Gateway Pro kit (Thermo Fisher Scientific). The resulting vector was transferred to Agrobacterium tumefaciens strain GV3101 and transformed into Col-0 ...
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bioRxiv - Microbiology 2022Quote: ... O-glycans were released from the mucins by reductive β-elimination and subjected to PGC-LC-ESI-MS/MS analysis according to a previously established protocol [66] using a Dionex Ultimate 3000 RS HPLC system coupled to a Thermo LTQ Velos Pro (Thermo Scientific, USA). O-glycan compositions were calculated from the masses using GlycoMod (https://web.expasy.org/glycomod/ ...
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bioRxiv - Systems Biology 2023Quote: ... The N-glycans were profiled in negative polarity mode on a Velos Pro linear ion trap connected to a Dionex Ultimate-3000 HPLC (Thermo Fisher Scientific) using an established PGC-LC-MS/MS method14 ...
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bioRxiv - Neuroscience 2024Quote: ... Mass spectrometry using LC-MS/MS was performed using Ultimate UHPLC system coupled to an Exploris 480 mass spectrometer with a FAIMS Pro interface (Thermo Fisher Scientific) The FAIMS compensation voltages were −45 and −65 V ...
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bioRxiv - Microbiology 2024Quote: The fractions were analyzed on an Orbitrap Fusion Lumos Tribrid mass spectrometer equipped with a FAIMS Pro ion mobility system and interfaced with an Easy-nLC1200 liquid chromatography system (all Thermo Fisher Scientific). Peptides were trapped on an Acclaim Pepmap 100 C18 trap column (100 μm x 2 cm ...
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bioRxiv - Microbiology 2023Quote: ... The samples were analysed by the Vanquish UHPLC system coupled with a Dual-Pressure Ion Trap Mass Spectrometer (Velos Pro, Thermo Fisher Scientific). Jasmonoyl-L-isoleucine (JA-Ile ...
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bioRxiv - Physiology 2024Quote: ... or phenylephrine positive control (10 μM) for 1 hour at the same time as Yo-Pro-1 dye (Thermo Fisher Scientific Y3603) (1μM ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting cDNA was then used for real-time qPCR with the Taq Pro Universal SYBR qPCR Master Mix (Vazyme, Q712-02) using the Quant Studio™ 6 Flex Real-Time PCR system (Thermo Fisher). The mRNA levels of GAPDH or actin were used as an internal control to normalize the mRNA levels of genes of interest ...
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bioRxiv - Molecular Biology 2024Quote: ... This system ran in-line with a Thermo Orbitrap Velos Pro hybrid mass spectrometer equipped with a nano-electrospray source (Thermo Fisher Scientific), and all data were collected in CID mode ...
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bioRxiv - Neuroscience 2024Quote: ... the blots were developed using Western Lightning enhanced chemiluminescence Pro (Revvity #NEL122001EA) or SuperSignal West Dura Extended Duration Substrate (ThermoFisher Scientific #37071) and were either exposed to HyBlot CL X-ray film (Thomas Scientific #1141J52 ...
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bioRxiv - Cell Biology 2024Quote: ... Liquid chromatography (LC)-coupled tandem MS/MS analysis was performed using a hybrid timed ion mobility-time of light mass spectrometer (timsTOF Pro 2, Bruker) coupled to a nanoflow chromatography system (Ultimate nanoSRLC, Thermo Fisher Scientific) equipped with a reversed phase-C18 column (Aurora Elite 250×0.075 mm ...
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bioRxiv - Cell Biology 2024Quote: ... The qPCR was conducted using the Taq Pro Universal SYBR qPCR Master Mix (Vazyme Biotech, China) in the ABI 7500 Fast Real-Time PCR System (Applied Biosystems, USA). The 2−△△CT method was used to calculate the relative expression (Livak & Schmittgen ...
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bioRxiv - Cell Biology 2024Quote: ... Mass spectrometric analysis of phosphorylated GST-yCTD was carried out in an AB Voyager-DE PRO MALDI-TOF (Brunker Corporation) with the 1:1 DHB matrix (Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2024Quote: ... The scaffolds were mounted on stubs using a carbon tape and imaged using an SEM (Phenom Pro X, Thermo Scientific, MA, USA).
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bioRxiv - Cell Biology 2024Quote: Each fraction was analyzed on an Orbitrap Lumos Tribrid mass spectrometer equipped with a FAIMS Pro ion mobility system interfaced with an nLC 1200 liquid chromatography system (all from Thermo Fisher Scientific). Peptides were trapped on an Acclaim Pepmap 100 C18 trap column (100 μm × 2 cm ...
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bioRxiv - Microbiology 2023Quote: ... or a Orbitrap Fusion Lumos equipped with a FAIMS Pro interface at 300 nl/minute via the analytical column using a Dionex Ultimate 3000 UPLC (Thermo Fisher Scientific). Ninety-five-minute analytical runs were undertaken by altering the buffer composition from 2% Buffer B (0.1% formic acid ...
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bioRxiv - Microbiology 2023Quote: ... Gene expression was quantified on QuantStudio 6 PRO Real-Time PCR System using PowerUp™ SYBR™ Green Master Mix (Applied Biosystems) and gene-specific primers (Table S4) ...
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bioRxiv - Systems Biology 2023Quote: ... ion source (ion spray voltage - 1.75V, heating temperature 285 °C) were analyzed using a Velos Pro Orbitrap mass spectrometer (Thermo Fisher Scientific, USA). In DDA-MS ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed using the Pro Taq HS premix Probe qPCR kit (Accurate Biology, China) and the QuantStudio5 system (Applied Biosystems, USA). The targeting gene for amplification of the ASFV genome was the conserved B646L gene segment ...
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bioRxiv - Systems Biology 2022Quote: LC-MS/MS analyses were performed on a TimsTOF Pro (Bruker) equipped with an UltiMate™ 3000 RSLCnano System (Thermo Fisher Scientific) run with an Aurora C18 column (Ion Opticks) ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 and LTQ-Orbitrap Velos Pro, Thermo Fisher Scientific) using a 124 min gradient ...
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bioRxiv - Systems Biology 2023Quote: Acquisition of single-cell derived peptides was conducted with an Orbitrap Astral mass spectrometer operated in positive mode with the FAIMS Pro interface (Thermo Fisher Scientific) using a compensation voltage set to −50 V ...
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Borrelia PeptideAtlas: A proteome resource of common Borrelia burgdorferi isolates for Lyme researchbioRxiv - Biochemistry 2023Quote: ... B31-Biotin labelled peptides were analyzed using Agilent 1100 nano pump coupled to an LTQ Velos Pro-Orbitrap Elite mass spectrometry (Thermo Scientific, USA). Sample was loaded onto a trap column consisting of a fritted capillary (360 μm o.d. ...
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bioRxiv - Physiology 2023Quote: ... was coupled to an Exploris 480 mass spectrometer equipped with a FAIMS pro interfaces and a Nanospray Flex ion source (all parts Thermo Fisher Scientific). Peptides were loaded onto a trap column (PepMap Acclaim C18 ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted peptides were subjected to electrospray ionization and then entered an LTQ Orbitrap Velos Pro ion-trap mass spectrometer (Thermo Fisher Scientific). Peptide sequences (and hence protein identity ...
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bioRxiv - Cell Biology 2023Quote: Each fraction was analysed on an Orbitrap Lumos Tribrid mass spectrometer equipped with the FAIMS Pro ion mobility system interfaced with an nLC 1200 liquid chromatography system (all from Thermo Fisher Scientific). Peptides were trapped on an Acclaim Pepmap 100 C18 trap column (100 μm × 2 cm ...
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bioRxiv - Microbiology 2023Quote: ... Eluted peptides were subjected to electrospray ionization and entered into an LTQ Orbitrap Velos Pro ion-trap mass spectrometer (Thermo Fisher Scientific). Peptides were detected ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were analyzed on an Orbitrap Exploris 480 connected to an EASY-nLC chromatography system using two compensation voltages applied with a FAIMS Pro Interface (Thermo Fisher Scientific). Proteins were identified in Proteome Discoverer 2.4 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... using a Vanquish Neo UHPLC System coupled to an Orbitrap Eclipse Tribrid Mass Spectrometer with FAIMS Pro Duo interface (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... or a Orbitrap Fusion Lumos equipped with a FAIMS Pro interface both at 300 nl/minute via the analytical columns using a Dionex Ultimate 3000 UPLC (Thermo Fisher Scientific). For samples infused into the Orbitrap Fusion Lumos ...
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bioRxiv - Developmental Biology 2023Quote: ... equipped with a Proxeon nanospray source or to an Orbitrap Eclipse Tribrid mass spectrometer equipped with a FAIMS pro interface and a Nanospray Flex ion source (all parts Thermo Fisher Scientific). Peptides were loaded onto a trap column (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: Rat lower esophageal lysates were prepared by homogenization (PRO Scientific Inc., Oxford, CT) in T-PER® Tissue Protein Extraction Reagent (ThermoFisher Scientific) with cOmplete™ EDTA-free protease inhibitor cocktail and PhosSTOP phosphatase inhibitors (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... was coupled to an Exploris 480 mass spectrometer equipped with a FAIMS pro interface and a Nanospray Flex ion source (Thermo Fisher Scientific). Peptides were loaded onto a trap column (PepMap Acclaim C18 ...
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bioRxiv - Cancer Biology 2023Quote: ... trypsinized and subjected to MS analysis to identify individual proteins using liquid chromatography-Tandem Mass Spectrometry LC-MS/MS (Orbitrap Velos Pro mass spectrometer, Thermo Fisher Scientific). The mass spectrometry analysis of posttranslational modification uses the same method and instrument.
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bioRxiv - Cell Biology 2023Quote: ... Eluted peptides were subjected to electrospray ionization (ESI) and then allowed to enter an LTQ Orbitrap Velos Pro ion-trap mass spectrometer (Thermo Fisher Scientific). Peptides were detected ...
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bioRxiv - Evolutionary Biology 2023Quote: The fractions were analyzed on an Orbitrap Eclipse Tribrid mass spectrometer equipped with the FAIMS Pro ion mobility system and interfaced with an Easy-nLC1200 liquid chromatography system (both Thermo Fisher Scientific). Peptides were trapped on an Acclaim Pepmap 100 C18 trap column (100 µm x 2 cm ...
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bioRxiv - Biochemistry 2023Quote: ... The reaction mixtures were heated from 10°C to 70°C for approximately 1 hr by a QuantStudio 7 Pro Real-Time PCR System (Thermo Fisher Scientific). Approximately 770 evenly spaced measurements of fluorescence against the temperature gradient were recorded and processed by QuantStudio V1.3 (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2023Quote: ... The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 and LTQ-Orbitrap Velos Pro, Thermo Fisher Scientific) using a 30 min gradient ...
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bioRxiv - Neuroscience 2023Quote: ... Neuron-cancer cocultures were quantified for neuronal outgrowth by identical image generation and processing with additional pre-incubation (3 drops/mL for 90 min at 37◦C) of NucRed Dead 647 ReadyProbes™ Reagent (TO-PRO-3 iodide) (Invitrogen, #R37113) for dead cells and NucBlue Live ReadyProbes™ Reagent (Hoechst 33342 ...
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bioRxiv - Neuroscience 2024Quote: ... At the end of each qPCR amplifications plots and melt curves (ΔRn vs cycle per well) were automatically calculated by the Quant Studio 7 Pro qPCR instrument (Thermo Fisher Scientific). Relative circRNA levels were quantified directly from the Ct average’s as shown before.17,20
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bioRxiv - Plant Biology 2024Quote: ... were used to amplify a 1:16 dilution of cDNA in triplicate in a Quantstudio™6 PRO real-time thermal cycler (ThermoFisher, Inc.) with a 61°C annealing temperature ...
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bioRxiv - Plant Biology 2024Quote: A mixture of standards (50 ng/µl of each standard in methanol) was analyzed using the Vanquish UHPLC system coupled with a Dual-Pressure Ion Trap Mass Spectrometer (Velos Pro, Thermo Fisher Scientific). Each compound was separated by an HSS T3 column (Waters ACQUITY HSS T3 100Å ...
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bioRxiv - Cell Biology 2024Quote: Both total proteome and phospho-enriched samples were injected multiple times with different methods and either with a normal nanospray ion source or a Field Asymmetric Waveform Ion Mobility Spectrometry interface (FAIMS pro, Thermo Fisher Scientific). For analyses with the normal nanoLC interface ...
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bioRxiv - Developmental Biology 2024Quote: ... to an Orbitrap Eclipse Tribrid mass spectrometer equipped with a FAIMS pro interface and a Nanospray Flex ion source (all parts Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2024Quote: ... was coupled to an Orbitrap ExplorisTM 480 mass spectrometer equipped with a FAIMS pro interface and a Nanospray Flex ion source (all parts Thermo Fisher Scientific).
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bioRxiv - Microbiology 2024Quote: ... Mass spectrometry using LC-MS/MS was performed using Ultimate UHPLC system coupled to an Exploris 480 mass spectrometer with a FAIMS Pro interface (Thermo Fisher Scientific) The FAIMS compensation voltages were-45 and-65 V ...
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bioRxiv - Microbiology 2024Quote: ... and then infused into an Orbitrap Fusion Lumos equipped with a FAIMS Pro interface at 300 nL/min via the analytical columns using a Dionex Ultimate 3000 UPLCs (Thermo Fisher Scientific). 125-minute analytical runs were undertaken by altering the buffer composition from 2% Buffer B (0.1% formic acid ...
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bioRxiv - Microbiology 2024Quote: ... the mass spectrometer was run with the HRMS1 method (Xuan et al., 2020) preceded by the FAIMS Pro Interface (Thermo Fisher Scientific) with a compensation voltage (CV ...