Labshake search
Citations for Thermo Fisher :
2151 - 2200 of 10000+ citations for Very Low Density Lipoprotein Receptor VLDLR Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2022Quote: ... The PEAK3-1xFLAG construct was transfected into 30 ml of Expi293F culture at a cell density of 4.0 × 106 cells/ml using ExpiFectamine 293 transfection reagent (Thermo Fisher Scientific Cat #A14524) according to the manufacturer’s protocol at 1 μg DNA / 1 ml ...
-
bioRxiv - Microbiology 2022Quote: ... RAW264.7 cells passaging was achieved by gentle cell scraping and seeding cells at a density of 3×106 cells/T75 flask (Nunc; Thermo Fisher Scientific).
-
bioRxiv - Molecular Biology 2023Quote: 293T-KO-2 cells were inoculated in 12-well culture plates at desired density and cultured in DMEM (11995065, Thermo Fisher, MA, USA) overnight ...
-
bioRxiv - Microbiology 2024Quote: Mid-log LdAQP-GFP cells at a density of approximately 3×106 cells per mL were incubated with Mitotracker (Invitrogen/Molecular Probes, #M7512) at a final concentration of 40 nM during 15 min at 27°C in culture medium without FCS ...
-
bioRxiv - Microbiology 2022Quote: ... activated cells were plated at a density of 3×105 cells/well in 96-well flat-bottom tissue culture plates in RPMI 1640 Media (Thermo Fisher Scientific #11875199) supplemented with 15% Fetal Bovine Serum (Thermo Fisher Scientific #A5256701) ...
-
bioRxiv - Pathology 2023Quote: ... The optical density at 600 nm (OD600) of the bacterial culture was measured by using a NanoDrop 2000c system (Thermo Fisher Scientific, USA) at 600 nm.
-
bioRxiv - Microbiology 2023Quote: ... Hungary) substrate and the optical density of the solution was measured at 450 nm using a Multiscan FC reader (Thermo Fisher Scientific Inc.). Blood samples were centrifuged after collection and the sera were kept at −70°C ...
-
bioRxiv - Microbiology 2022Quote: ... and the signal intensities [the optical density at 450 nm (OD450)] were read with a Multiskan Sky plate reader (Thermo Fisher Scientific, China). Similarly ...
-
bioRxiv - Cell Biology 2023Quote: ... using either a WPA CO8000 Cell Density Meter (Biochrom, Cambridge, UK) or a Multiskan FC microplate reader with a 594 nm filter (Thermofisher, Waltham, MA, USA).
-
bioRxiv - Cancer Biology 2023Quote: THP–1 cells were maintained at a density of 1×106 cells/ml in RPMI 1640 Medium (ThermoFisher, Waltham, MA, Cat. No. 11835055) supplemented with 10% fetal bovine serum (FBS) ...
-
bioRxiv - Neuroscience 2023Quote: ... and sample optical densities at 450 and 570 nm were quantified using a microplate spectrophotometer (Varioskan LUX; Thermo Fisher Scientific, Waltham, MA, USA). The OD value for each diluted sample was calculated by subtracting the measured value at 570 nm from that at 450 nm to correct for optical defects.
-
bioRxiv - Immunology 2023Quote: BMDMs were prepared and seeded into 12-well cell culture plate or 96-well cell culture plate at a density of 1 x 106 cells/mL of RPMI-1640 medium (Thermo scientific, Cat# 61870036) containing 10% of Fetal Bovine Serum (FBS ...
-
bioRxiv - Cell Biology 2022Quote: ... for 3 days at a density of 5 × 104 cells per well in a 96-well tissue culture plate (Thermo Fisher, Nunclon delta). The media was then replaced with fresh PMA-free media and the cells were left to rest for 24 h before the infection protocol ...
-
bioRxiv - Biophysics 2022Quote: ... Cells were seeded at a density of 3· 105/cm2 the day before the experiments in Fluorobrite DMEM (A1896701, by Life Technologies SAS-Thermo Fisher Scientific).
-
bioRxiv - Microbiology 2023Quote: ... which was quantified using both optical density at 600 nm (OD600) and total protein content estimated using a BCA protein assay kit (Thermo Scientific, MA, USA). For quantification of the expression of the arsM gene ...
-
bioRxiv - Microbiology 2023Quote: ... RAW264.7 cells passaging was achieved by gentle cell scraping and seeding cells at a density of 3×106 cells/T75 flask (Nunc; Thermo Fisher Scientific).
-
bioRxiv - Biophysics 2023Quote: FACS-purified MΦTR were seeded onto 15-mm glass coverslips in 24-well plates at a density of 20,000 cells/mL in 1 mL Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal calf serum and 1% penicillin/streptomycin ...
-
bioRxiv - Cell Biology 2024Quote: ... was used to measure collagen in lung lobes following kit instructions and optical density was measured using a Varioskan LUX plate reader (Thermo Fisher Scientific, VL0000D0).
-
bioRxiv - Immunology 2021Quote: ... and gene expression was assayed using quantitative reverse transcription PCR using PerfeCTa SYBR Green Fastmix Low ROX (Quanta) on the QuantStudio 12K Flex (Applied Biosystems) with primers (Integrated DNA Technologies ...
-
bioRxiv - Cell Biology 2019Quote: The ASCs were seeded at a density of 2,5.104 cells/cm2 and after 72 h were induced to differentiate to osteoblasts by adding ODM containing DMEM with low glucose (Thermo Fisher) supplemented with 10% FBS (Internegocios) ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were dissociated and 9×103 cells were plated into each well of a low attachment U-bottom 96-well-plate in 8GMK medium consisting of GMEM (Thermo Fisher), 8% KSR (Thermo Fisher) ...
-
bioRxiv - Developmental Biology 2020Quote: ... All constructs were verified by Sanger sequencing and midipreped for electroporation and transfection using PureLink Fast Low-Endotoxin Midi Kit (Invitrogen, A36227).
-
bioRxiv - Molecular Biology 2021Quote: ... Protein concentration in both low-salt and chromatin nuclear fractions was determined at 280 nm using a Nanodrop spectrophotometer (ThermoFisher Scientific). Thereafter ...
-
bioRxiv - Developmental Biology 2022Quote: ... Embryos were anaesthetized with 0.28 - 0.4μg/μl of MS-222 before imaging and mounted in 100μl of 0.6% ultrapure low melting point (LMP) agarose (Invitrogen REF 16500-500). Fixed and immunostained embryos were mounted in 100μl of 1% ultrapure LMP agarose ...
-
bioRxiv - Genomics 2020Quote: ... we digested about 1μg of high-quality and high-concentration genomic DNA with a low frequency restriction enzyme (‘Sbf1’, Thermo Fisher Scientific). We ligated the digested fragments to a modified Solexa adaptor P1 with a unique 5bp barcode and pooled the DNA fragments from individuals bearing unique barcode identifiers for further fragmentation using a COVARIS S220 Focused-ultrasonicator (Woburn ...
-
bioRxiv - Immunology 2021Quote: ... For markers displaying low surface expression levels unstained and single colour stained UltraComp eBeads (Thermo Fisher Scientific Inc.; #01-2222-42) were used for the calculation of compensation matrixes ...
-
bioRxiv - Microbiology 2019Quote: L-WNT 3A expressing cell lines were used to produce WNT conditioned media as a component of the proliferation media and were propagated in low glucose DMEM (Life technologies), 10% fetal calf serum (FCS) ...
-
bioRxiv - Microbiology 2019Quote: ... the suspension was further diluted 1:50 in growth medium and mixed 1:1 with 1% agarose (TopVision Low Melting Point Agarose, Fisher Scientific). Each chamber in the fluidic chip was loaded with 5 μL bacteria-agarose and incubated at 4 °C for 5 min to solidify the agarose gel ...
-
bioRxiv - Bioengineering 2021Quote: ... differentiation was performed during 40 days in liver differentiation medium (LDM) containing to comprise 500ml total volume: 285 ml of DMEM low glucose (Invitrogen 31885023), 200 ml of MCDB-201 solution in water (Sigma M-6770 ...
-
bioRxiv - Genomics 2021Quote: ... cells were dissociated and 9×103 cells were plated into each well of a low attachment U-bottom 96-well-plate in 8GMK medium consisting of GMEM (Thermo Fisher), 8% KSR (Thermo Fisher) ...
-
bioRxiv - Biochemistry 2020Quote: ... Tissues were finally suspended in 1% (w/v) low-melting agarose at ~40°C mixed with Slowfade Diamond oil (Molecular Probes) on a slide ...
-
bioRxiv - Bioengineering 2020Quote: ... The cells were incubated at 37°C in an atmosphere of 5% CO2 in air in Low-glucose Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco, Invitrogen, Karlsruhe, Germany) supplemented with 10% heat inactivated Fetal Bovine Serum (FBS ...
-
bioRxiv - Neuroscience 2021Quote: ... Brain lysates from transgenic UBQLN2-low mice [22] were pre-cleared with 0.1 volume of a protein A agarose beads slurry (ThermoFisher Cat# 20334) rotating at 4°C for 1 hour ...
-
bioRxiv - Cell Biology 2021Quote: ... Droplets were incubated at 37°C for 20 mins followed by addition of MEL medium (50% neurobasal, 30% low glucose DMEM (Life Technologies), 20% MCDB201 (Sigma) ...
-
bioRxiv - Neuroscience 2020Quote: Skin fibroblasts of patients or healthy controls were cultured at low passage number and maintained with Dulbecco’s modified eagle medium (41965-062, Thermo Fisher Scientific) supplemented with 15% fetal bovine serum (10270106 ...
-
bioRxiv - Molecular Biology 2020Quote: ... with the following modifications for likely partially degraded samples with an expected low target viral content: 50uL Dynabeads® M-270 Streptavidin beads (Invitrogen) instead of 30 uL Dynabeads® MyOne™ Streptavidin C1 (Invitrogen) ...
-
bioRxiv - Bioengineering 2021Quote: MDCK.2 wild type canine kidney epithelial cells (ATCC) were cultured in customized media consisting of low-glucose (1 g/L) DMEM with phenol red (Gibco, USA), 1 g/L sodium bicarbonate ...
-
bioRxiv - Immunology 2020Quote: ... Tubes containing the swab and VTM were pulse-vortexed three times and 1.5mL aliquots were collected onto low protein binding tubes (ThermoFisher cat# 88379). Tubes were then centrifuged at 4⁰C for 10 minutes at 1500g ...
-
bioRxiv - Immunology 2021Quote: ... were lysed by 2 cycles of freeze-thaw and cellular debris pelleted by low speed centrifugation at 2000 x g (Sorvall Legend XTR, Thermo Scientific) for 10 minutes at 4°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... MSCs were cultured in GM made of low-glucose Roswell Park Memorial Institute (RPMI-1640) supplemented with GlutaMAX and HEPES (both Life Technologies), 10% FBS and 1× Ab-Am ...
-
bioRxiv - Microbiology 2020Quote: ... The wild type and derivative bteA alleles were cloned downstream from the GAL1 promoter in frame with the C-terminal GFP gene in a low copy centromeric vector pYC2-CT (Invitrogen, USA) carrying the URA3 selection marker ...
-
bioRxiv - Biochemistry 2022Quote: ... Each resolubilized sample was then transferred to 1.5 mL eppendorf low-adhesion tubes containing 1 mL PBS with 30 µL high-capacity streptavidin resin (Thermo Scientific 20357) to give a final SDS concentration of 0.2% ...
-
bioRxiv - Developmental Biology 2022Quote: ... Viral supernatant was collected and concentrated after 48 hours and transduced directly into hiPSCs at low to mid-confluency along with 8 μg/mL polybrene (Fisher Scientific). Because all plasmids contained puromycin-resistance cassettes ...
-
bioRxiv - Cell Biology 2022Quote: ... For markers displaying low surface expression levels unstained and single colour stained UltraComp eBeads (Thermo Fisher Scientific Inc.; #01-2222-42) were used for the calculation of compensation matrixes ...
-
bioRxiv - Molecular Biology 2022Quote: βTC-6 cells (43) (ATCC, Manassas, VA) were cultured in low glucose DMEM (Dubelco’s Modified Eagle’s Medium) (Fisher Scientific, Waltham, MA) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Cancer Biology 2022Quote: ... with the following modifications (1) antigen retrieval was performed using low pH IHC Ag Retrieval Solution (Thermo Fisher, 00-4955-58) and subjected to heat using a steamer for 40 minutes ...
-
bioRxiv - Biophysics 2022Quote: ... H2B-Halo-SNAP U2OS cells (kind gift from the Tjian-Darzacq laboratory, UC Berkeley, CA) were grown in low glucose DMEM medium (ThermoFisher #10567014) supplemented with 10% FBS ...
-
bioRxiv - Genomics 2022Quote: ... Crosslinks were reversed by incubating at 65°C overnight and Low Melting Point Agarose were digested by Agarase (Thermo Fisher Scientific) 1 hr at 42°C ...
-
bioRxiv - Cell Biology 2022Quote: ... transferred in media containing either low arginine concentrations (1%) or fully reconstituted supplemented with 10% dialyzed FBS (Gibco cat. 26400-044) (50×106 cells/condition ...
-
bioRxiv - Cancer Biology 2022Quote: ... The dried samples were reconstituted in 0.1% TFA and separated using a second dimension low pH gradient using a nanoflow liquid chromatography (Thermo Easy nLC 1000, Thermo Scientific) coupled to a mass spectrometer at 300 nl/min ...