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Citations for Thermo Fisher :
2051 - 2100 of 10000+ citations for Very Low Density Lipoprotein Receptor VLDLR Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Low-passage E14 ESCs were transfected with pX458 plasmid and the corresponding ssODN donor using Lipofectamine 2000 or 3000 (Invitrogen). After GFP fluorescence-based FACS sorting of transfected single cells ...
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bioRxiv - Neuroscience 2021Quote: ... Two-round T7-RNA polymerase-mediated linear amplification was performed according to optimized protocols for low-input RNA amounts (Small Sample Target Labeling Assay Version II, Affymetrix). Biotin-labeled second-round aRNA was generated with an NTP-mix containing Biotin-11-CTP and Biotin-16-UTP (PerkinElmer ...
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bioRxiv - Neuroscience 2021Quote: ... Hybridomas were maintained in DMEM high glucose supplemented with 10% Ultra-low IgG Fetal Bovin Serum (FBS) and Penicillin/Streptomycin (Gibco) and filtered through a 0.2μm filter ...
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bioRxiv - Microbiology 2020Quote: ... and HEK293S GnTI-/- cells (ATCC CRL-3022) in Freestyle 293 Expression Medium supplemented with 2% ultra-low IgG FBS (Gibco). We maintained ...
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bioRxiv - Cancer Biology 2022Quote: Peritoneal lavage fluids were concentrated by ultrafiltration through a low-adsorption polyethersulfonate (PES) membrane (mol. mass. cutoff 3kDa, concentrator Pierce PES 3K, Thermofisher). The average concentration factor was 4.2 with a range from 3.1– 5 and it was used to calculate non-concentrated levels ...
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bioRxiv - Cell Biology 2022Quote: ... and placed into the standard colony assay at a concentration of 1 cell/unit per well in a low-attachment Nunclon 96-well plate (ThermoFisher). Colonies were counted 2 weeks after plating ...
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bioRxiv - Biophysics 2022Quote: U2OS NUP96-SNAP cells (23) were cultured in DMEM growth medium with low glucose and without phenol red (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... Liver spheres were collected and sorted in low glucose DMEM (1g/L D-Glucose/L-Glutamine, Pyruvate, Gibco #31885-023). The same filtering approach was used for Crohn’s disease biopsies with a lower size strainer of 200 μm and a higher size strainer of 500 μm resulting in a suspension of spheres between 200-500 μm ...
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bioRxiv - Genomics 2022Quote: ... Liver dissociation buffer was a mix of low glucose DMEM (1g/L D-Glucose/L-Glutamine, Pyruvate, Gibco #31885-023), 15mM HEPES (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... Plasmid DNA (5 μg) was added to 1.5 × 106 viable hBMSCs-derived cells in 0.2-ml DMEM low glucose medium (Thermo Fisher Scientific) before electrical pulsing.
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bioRxiv - Neuroscience 2022Quote: Larval fish (4-7 dpf) were mounted in a small drop of 2% low melting point agarose (ThermoFisher Scientific 16520) in the center of the uncoated side of a mirror galvanometer (Thorlabs GVS0111) ...
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bioRxiv - Microbiology 2022Quote: ... Vector propagation was performed in 1.5 mL LB (low salt + Zeocin 75 μg mL−1) and GeneJET PCR Purification Kit (ThermoFisher, Germany). P ...
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bioRxiv - Neuroscience 2022Quote: ... Worms were immobilized with 20 mM sodium azide and mounted in a 1% low-melting-point agarose gel (UltraPure, Invitrogen). The TagBFP ...
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bioRxiv - Microbiology 2022Quote: ... The samples from each species were pooled and filter-sterilized through a 0.22 µm low-binding PES membrane using Rapid Flow Rapid-Flow Sterile Disposable Filter Units (Nalgene/Thermo Scientific). The samples were frozen at −80 °C and freeze dried ...
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bioRxiv - Microbiology 2022Quote: ... rinsed with PBS and kept at 37°C in low-glucose (1 g/L) DMEM (Gibco, Thermo Fisher Scientific Inc.) without serum until processing ...
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bioRxiv - Microbiology 2022Quote: ... rinsed with PBS and kept at 37°C in low-glucose (1 g/L) DMEM (Gibco, Thermo Fisher Scientific Inc.) without serum until processing ...
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bioRxiv - Developmental Biology 2022Quote: ... all cell aggregates were collected and individually transferred to a new 96-well U-bottom plate with a super-low cell attachment surface in 100 µl of E6 medium (A1516401, Gibco) with Normocin (concentration 100 µg/ml) ...
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bioRxiv - Bioengineering 2023Quote: ... EGFP) embryos (48 hpf) were anesthetized in 0.005% tricaine (MS222) and mounted in 1% low melting point agarose (Thermo Scientific) in a glass capillary (50 µl ...
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bioRxiv - Bioengineering 2023Quote: ... Thirty minutes post injections one successfully injected embryo per group was anesthetized in 0.005 % tricaine (MS222) and mounted on a lateral position in 1% low melting point agarose (Thermo Scientific) on a cover-glass bottomed petri-dish (MatTek) ...
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bioRxiv - Neuroscience 2024Quote: ... in PBST at 4 °C over night and mounted in 1.5 % low melting point agarose (Thermo Fisher Scientific, Waltham, USA). In-vivo imaging was performed on larvae embedded in 1.5 % low melting point agarose in E3 medium supplemented with 4.2 g/l MS-222 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: Retroviral particles were produced by transfecting pLPC plasmids into low passage Phoenix cells (ATCC) at 90% confluence using Lipofectamine3000 (ThermoFisher) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The spheroplasted cells were then mixed with an equal volume of molten low-melting point agarose (2%; Thermo Scientific, R0801) in 0.25 M EDTA and 1.2 M sorbitol ...
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bioRxiv - Immunology 2024Quote: ... A blunted 22G needle was then inserted via a tracheostomy and used to inflate the lungs slowly but continuously with 1 mL of 2% low-melting point agarose (ThermoFisher) prepared in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmid DNA (5 μg) was added to 1.5 × 106 viable hMB-MSCs in 0.2-ml DMEM low glucose medium (Thermo Fisher Scientific) before electrical pulsing.
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bioRxiv - Cell Biology 2024Quote: ... 1000 to 5000 cells/well were re- aggregated in low-cell-adhesion Nunclon™ Sphera™ 96 well Microplates (ThermoFisher) that contained serum-free HO growth and differentiation medium ...
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bioRxiv - Genomics 2023Quote: MCF7 cells (>2 × 108 cells/replica for 3 biological replica) were grown for 48 hrs in DMEM (low glucose, pyruvate, no glutamine, and no phenol red) (Gibco) supplemented with 5% Charcoal Striped Serum (Biowest ...
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bioRxiv - Immunology 2023Quote: ... The purified pooled library was then quantified with a gel-based method using the Low- Range Quantitative Ladder (Life Technologies), dsDNA High-Sensitivity Qubit (Life Technologies) ...
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bioRxiv - Developmental Biology 2023Quote: ... the primed neural crest cell suspensions are pelleted at 300xg for 2min and resuspended in melanocyte induction media (MIM) (50% Neurobasal, 30% Low glucose DMEM/F12 (Gibco), 20% MCDB201 base medium (Sigma) ...
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bioRxiv - Immunology 2022Quote: ... Serum was serially diluted in Roswell Park Memorial Institute media supplemented with 4% Ultra-Low IgG FBS (Assay buffer; Gibco). Assay buffer and diluted serum was added to transfected cells ...
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bioRxiv - Cancer Biology 2023Quote: ... the transfected cells were trypsinized and clustered in 96-well Nunclon Sphera U-bottom low adhesion plates (Thermo Scientific, 174925) overnight (1000 cells/well) ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from 14 high-TADS (TADS ≥ 39) and 16 low-TADS (TADS ≤ 10) samples (10 million spermatozoa per sample) by TRIzol LS (Invitrogen) containing tris(2- carboxyethyl)phosphine (TCEP ...
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An updated C. elegans nuclear body muscle transcriptome for studies in muscle formation and functionbioRxiv - Genetics 2023Quote: ... The libraries were depleted of ribosomal RNA contaminants using the Low Input RiboMinus Eukaryotic System v2 (ThermoFisher Scientific Catalog # A15027). The libraries were prepared from the ribosomal RNA depleted samples using the Nextflex Rapid Directional RNAseq Kit (PerkinElmer Catalog # NOVIA-5138-08 ...
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bioRxiv - Biochemistry 2023Quote: ... Samples containing low amount of miRNA were precipitated by using isopropanol in presence of glycoblue co-precipitant (Thermofisher Scientific, USA). cDNA was prepared by taking 100-200ng of RNA from cellular RNA samples or by taking equal volume of RNA isolated from in vitro assay samples or EV samples by using a Taqman reverse transcription kit (Applied Biosystems) ...
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bioRxiv - Biochemistry 2023Quote: ... FZD3 and FZD6 protein were thawed and washed with a low-salt buffer (10 mM HEPES pH 7.5, 20 mM KCl, 10 mM MgCl2, protease inhibitor cocktail (Thermo Fisher)) ...
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bioRxiv - Cell Biology 2023Quote: ... The gotten red blood cells were seeded in DMEM low glucose with 10% FBS (Gibco, Life Technologies Ltd, Paisley, UK) at a density of 160,000/cm2 ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentrations were measured by BCA and samples were diluted to 1 mL of 0.5 mg/mL in RIPA buffer in low protein binding collection tubes (Thermo Fisher) containing 25 μL of NanoLink® magnetic streptavidin beads ...
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bioRxiv - Microbiology 2023Quote: ... or in a semi-defined medium (SDM) supplemented with low (1.7uM) or high (75uM) concentrations of MnSO4•H2O (Acros Organics). S ...
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bioRxiv - Cancer Biology 2023Quote: ... For all other cell lines spheroid growth was assessed in ultra-low attachment 96-well round bottomed plates (Corning Costar #7007, S-BIO PrimeSurface #MS-9096UZ, or Nunc Nucleon Sphera microplates ThermoFisher # 174929) ...
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bioRxiv - Developmental Biology 2023Quote: ... and 2000 cells in 150 µl of media were plated in each well of 96-well ultra-low attachment plates (174929, Thermofisher). Cells were centrifuged at 100g for 3 min and incubated at 37°C (in 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... The gotten red blood cells were seeded in DMEM low glucose with 10% FBS (Gibco, Life Technologies Ltd, Paisley, UK) at a density of 160,000/cm2 ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: Psychometric verification of labyrinthectomy was performed by mounting each larva in 2% low-melting point agarose (Thermo Fisher Scientific 16520) on the unmirrored side of a galvonometer (GVS011 ...
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bioRxiv - Bioengineering 2023Quote: ... supplemented with Low Serum MSC Growth Kit for adipose MSCs (ATCC PCS-500-040) and Antibiotic-Antimycotic (A-A; Invitrogen) resulting in final complete MSC media concentrations of 2% Fetal Bovine Serum (FBS) ...
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bioRxiv - Bioengineering 2023Quote: ... The cells self-aggregate and were differentiated for 21 days in a serum-free chondrogenic medium (CM) containing low glucose DMEM (Gibco) supplemented with 1% antibiotic– antimycotic (Invitrogen) ...
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bioRxiv - Cancer Biology 2023Quote: ... The patient-derived low-passage MM cell lines (a gift from G.-E. Ghanem) were grown in F-10 (Gibco BRL Invitrogen), supplemented with 10% FBS (Gibco BRL Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... all the samples were minced immediately and digested in type I collagenase (1mg/ml, Gibico) and type II collagenase (1mg/ml, Gibico) diluted in low-glucose DMEM (Gibco) solution at 37 °C for 30-40 min ...
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bioRxiv - Cell Biology 2023Quote: ... Bio-techne) seeded on 12-well Greiner Cell STAR multiwell low retention plates with ENR medium (Advanced DMEM/F12 (Gibco), 10 mM Hepes (15630056) ...
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bioRxiv - Bioengineering 2023Quote: ... KTB21 human mammary basal epithelial cell line was cultured in epithelial cell growth medium (DMEM [low glucose]: Ham’s F12 [1:3] medium supplemented with 5% FBS [Thermo Fisher Scientific] ...
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bioRxiv - Cancer Biology 2023Quote: ... MSCs were passaged twice a week in DMEM low glucose/pyruvate/HEPES medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 15% fetal calf serum (FCS ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 mL of liquid was placed in the center of a Petri dish containing 30 mL of 3 % Low Melting Point agarose (LMP agarose, Invitrogen). LMP agarose plates with hydrated individuals (approx ...
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bioRxiv - Neuroscience 2023Quote: ... and then embedded in a 30% sucrose solution at low temperature in a cryostat (Microm HM525, Thermo Scientific, MA, USA). Using the Allen mouse brain atlas as reference ...